Rroxscaffold_3G00274340

RNA-binding protein NOB1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001896
Physical Location & Seq
Forward (+)
66048267 .. 66050698
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274340.1

Sequence Viewer

Length: 690 bp
ATGGAGCCCTCCCCTGAATCCCGCGACAAAGTTGAAAAGTTTGCGAGGGCTACTGGGGATTTCCAGTCACTGTCAGAGGTTGATATTCGTCTTATTGCTATGACATATACACTGGAGGCTCAGATACATGGCACTGAGCACCTCAGGGACTGTCCTCCGCCGGTGCATACTGTCAATGTCAGGAGGTTGCCGGAGAAGGACTTGCCCGGCTGGGAAAATAATGTCCCCAATTTGGAAGAGTGGGAAGCGCTGGAAAACAAAGCGGAGGATAAGAGCAACTCTGGTTCGAGGATTCTTCCGTTGAAAGATATCAACTTGAATGTTTCTGCGGTGGAAATTAGAAGTGATGTTCATTCTGAGAATCAGGAGGGTGGTGAGGGCATTCAGGGGAGATATAGGAGATATTTTCCAAAGAAGAAAGAGATAAATATTGAAGGGAAGATGGTTTCTGATGGAATTGATGCGTCCCAAGGACAAGTTGATGATAATACTGGTGACTGGATGCCTGCTGTTAGTCGGAGTACACATAGAAGGTTTTTGAGAAGAAAAGCTAGACGTGAGTCTTACGAGGCGGCATCAGCTGATAATGATCCCGCTTTGCTAATATTCGTGCTTCAAGGTTTTCTGAAACACCCTGTGCATAAGATGATGTTTTATGAAGATGACTTGCATATAAGTTGCTTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
NOB1_Zn_bind PIN_6

Protein Analysis

229

Amino Acids

26.11

Weight (kDa)

5.54

Isoelectric Point (pI)

66.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIN_6 PF17146 2 - 38 3e-10 PIN domain of ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 24
AciI CCGC 6 cut(s) 22, 158, 263, 329, 572, 594
AclWI GGATC 1 cut(s) 584
AdeI CACNNNGTG 1 cut(s) 637
AfaI GTAC 1 cut(s) 523
AfeI AGCGCT 1 cut(s) 249
AfiI CCNNNNNNNGG 1 cut(s) 232
AgsI TTSAA 5 cut(s) 35, 304, 319, 434, 617
AjiI CACGTC 1 cut(s) 557
AluBI AGCT 2 cut(s) 551, 581
AluI AGCT 2 cut(s) 551, 581
Alw21I GWGCWC 1 cut(s) 141
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 2 cut(s) 70, 150
Aor51HI AGCGCT 1 cut(s) 249
AspLEI GCGC 1 cut(s) 250
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 386, 506
AxyI CCTNAGG 1 cut(s) 143
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 141
BccI CCATC 2 cut(s) 436, 446
BcnI CCSGG 1 cut(s) 207
BfaI CTAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 251
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 1 cut(s) 207
BmgBI CACGTC 1 cut(s) 557
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 207
BmrI ACTGGG 1 cut(s) 63
BmsI GCATC 3 cut(s) 451, 492, 584
BmuI ACTGGG 1 cut(s) 63
BoxI GACNNNNGTC 1 cut(s) 559
BpmI CTGGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 1 cut(s) 469
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 5 cut(s) 58, 64, 117, 496, 503
Bse21I CCTNAGG 1 cut(s) 143
Bse8I GATNNNNATC 1 cut(s) 588
BseDI CCNNGG 1 cut(s) 469
BseGI GGATG 1 cut(s) 507
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 4 cut(s) 126, 134, 157, 348
BseNI ACTGG 5 cut(s) 58, 64, 117, 496, 503
BseYI CCCAGC 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 24
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 3 cut(s) 161, 191, 207
BslFI GGGAC 3 cut(s) 161, 209, 451
BslI CCNNNNNNNGG 1 cut(s) 232
BsmFI GGGAC 3 cut(s) 161, 209, 451
BsmI GAATGC 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 9, 141
Bsp143I GATC 1 cut(s) 589
BspACI CCGC 6 cut(s) 22, 158, 263, 329, 572, 594
BspCNI CTCAG 4 cut(s) 127, 133, 156, 349
BspFNI CGCG 1 cut(s) 24
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 584
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 5 cut(s) 58, 64, 117, 496, 503
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 469
BssMI GATC 1 cut(s) 589
BssT1I CCWWGG 1 cut(s) 469
Bst4CI ACNGT 3 cut(s) 72, 152, 172
Bst6I CTCTTC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 507
BstDEI CTNAG 4 cut(s) 120, 135, 143, 357
BstF5I GGATG 1 cut(s) 507
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 1 cut(s) 592
BstMBI GATC 1 cut(s) 589
BstMWI GCNNNNNNNGC 1 cut(s) 578
BstPAI GACNNNNGTC 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 205
BstUI CGCG 1 cut(s) 24
Bsu36I CCTNAGG 1 cut(s) 143
BtrI CACGTC 1 cut(s) 557
BtsCI GGATG 1 cut(s) 507
BtsIMutI CAGTG 3 cut(s) 68, 110, 132
Cac8I GCNNGC 1 cut(s) 507
CaiI CAGNNNCTG 2 cut(s) 70, 150
CfoI GCGC 1 cut(s) 250
Cfr10I RCCGGY 1 cut(s) 160
CseI GACGC 1 cut(s) 453
Csp6I GTAC 1 cut(s) 522
CviAII CATG 1 cut(s) 128
CviJI RGCY 6 cut(s) 7, 50, 119, 210, 551, 581
CviKI_1 RGCY 6 cut(s) 7, 50, 119, 210, 551, 581
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 4 cut(s) 120, 135, 143, 357
DpnI GATC 1 cut(s) 591
DpnII GATC 1 cut(s) 589
DraIII CACNNNGTG 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 1 cut(s) 147
Eco130I CCWWGG 1 cut(s) 469
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 310
Eco47III AGCGCT 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 143
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 1 cut(s) 131
FaqI GGGAC 3 cut(s) 161, 209, 451
FatI CATG 1 cut(s) 127
FauI CCCGC 2 cut(s) 29, 601
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 1 cut(s) 514
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 1 cut(s) 552
GlaI GCGC 1 cut(s) 249
GluI GCNGC 1 cut(s) 573
GsaI CCCAGC 1 cut(s) 214
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 251
HapII CCGG 3 cut(s) 161, 191, 207
HgaI GACGC 1 cut(s) 453
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 131
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 4 cut(s) 17, 292, 361, 560
HpaII CCGG 3 cut(s) 161, 191, 207
HphI GGTGA 2 cut(s) 386, 506
Hpy166II GTNNAC 1 cut(s) 524
Hpy188I TCNGA 6 cut(s) 76, 123, 358, 451, 519, 627
Hpy188III TCNNGA 2 cut(s) 181, 365
Hpy8I GTNNAC 1 cut(s) 524
HpyAV CCTTC 3 cut(s) 190, 428, 525
HpyCH4III ACNGT 3 cut(s) 72, 152, 172
HpyCH4IV ACGT 1 cut(s) 556
HpyCH4V TGCA 3 cut(s) 166, 640, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 578
HpyF3I CTNAG 4 cut(s) 120, 135, 143, 357
HpySE526I ACGT 1 cut(s) 556
Hsp92II CATG 1 cut(s) 131
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 1 cut(s) 589
LmnI GCTCC 1 cut(s) 4
LweI GCATC 3 cut(s) 451, 492, 584
MaeI CTAG 1 cut(s) 552
MaeII ACGT 1 cut(s) 556
MaeIII GTNAC 2 cut(s) 66, 494
MalI GATC 1 cut(s) 591
MboI GATC 1 cut(s) 589
MboII GAAGA 6 cut(s) 248, 287, 427, 451, 555, 671
MhlI GDGCHC 2 cut(s) 9, 141
MluCI AATT 3 cut(s) 229, 336, 456
MlyI GAGTC 1 cut(s) 569
MmeI TCCRAC 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 581
MspI CCGG 3 cut(s) 161, 191, 207
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 381
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 578
NciI CCSGG 1 cut(s) 207
NdeII GATC 1 cut(s) 589
NlaIII CATG 1 cut(s) 131
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 66, 494
PctI GAATGC 1 cut(s) 381
PfeI GAWTC 3 cut(s) 17, 292, 361
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
PshAI GACNNNNGTC 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 2 cut(s) 70, 150
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 1 cut(s) 589
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 2 cut(s) 9, 141
SetI ASST 8 cut(s) 81, 144, 188, 536, 553, 559, 583, 622
SfaNI GCATC 3 cut(s) 451, 492, 584
SgrAI CRCCGGYG 1 cut(s) 160
Sse9I AATT 3 cut(s) 229, 336, 456
SsiI CCGC 6 cut(s) 22, 158, 263, 329, 572, 594
SspI AATATT 2 cut(s) 430, 606
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 3 cut(s) 72, 152, 172
TaiI ACGT 1 cut(s) 559
TaqI TCGA 1 cut(s) 287
TasI AATT 3 cut(s) 229, 336, 456
TatI WGTACW 1 cut(s) 521
TauI GCSGC 1 cut(s) 575
TfiI GAWTC 3 cut(s) 17, 292, 361
TscAI CASTG 3 cut(s) 75, 117, 139
TseFI GTSAC 2 cut(s) 66, 494
Tsp45I GTSAC 2 cut(s) 66, 494
TspDTI ATGAA 2 cut(s) 341, 672
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 3 cut(s) 75, 117, 139
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.