pycom10g26580

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000635
Physical Location & Seq
Reverse (-)
27881364 .. 27883183
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26580.1

Sequence Viewer

Length: 1380 bp
ATGATTAGAGATCAAAACATGGTTAATTTCCCTAGTGTTCCGAAAACAATCTCTCTTTTTTTGCTTTGTTTTCTCATCACCCTCCTTTTTGATCTTGCTTATTCTGATCCTGATCCTCCATACAAGTTGTGCTCAGATGGACGCGGTTATGTCGAAAATAGTACGTTTCAGAAAAACCTCCATAGCCTGCTCAATTCATTGCCTTCAATTGCTTCTGTCTCCAAGCTGTACAATACTTCCGCCGGAATTGAACCAGATAGAATTTATGGCCTTTACATGTGCCTCAATTATGTTACGAATGAGATATGTCACAACTGCATCAACACCGCGCATTTAGACATTGTTAGGATTTGTCCCAACTCAACAGAATCAGTTTTGTGGGAGGAAACGTGCCAACTGCGCTACTCCAATGTGAAGTTCTATGGTGAATTGAATGTGGCAGACAACATTGTGTCCGCTAACAAGAAAAACGTTTCAGACCCAAAAAAGTTTGAGAATACCGTAAAGGAGAAGCTGAGGGAGCTTACGAAGCAAGCGGCATATAATGCTTCAGCGAAAATGTATGCTACTGGAGATGTACAATTTGAAGATAAAGTGATATATGCTCTTGTGCAGTGCACTAGAGACTTATCTGGAGATGACTGTGACAAATGCCTTCGGCACGCCATAGAAGACGTTTTGAGAAAGTATTATTTCTCCATTGGTGCGCGGCTTATGAGCCGGAGTTGCTATCTGAGGTACGAGTTGTATGGCTTCTATAATGGTGCAACTAATGCTTCTATTTCCAGCTCTCCAAATAACAACAAAGGGGACGGTAGAGGATGGCGGATCGGAATCCTTACGGTTGTGTCAGCATGTCTAGCCATACTACTTTTGGGTTCCTGTGTCTATCTTGCAATAAGAAAAAGAAACAAAAAGGGGAGTAGTGAGATTTTAAGACAACATGTATTGTTCCCGGACCGAAGTTTTCAAGGCAGAAACCCGGAAGCTGAGGAATACCCCTATATCAGTTTGGCATCTATAAATGCAGCTACGAATAAATTTTCTGATTCAAGTAAGCTCGGAGAAGGTGGTTTTGGGCCTGTTTACAAGGGTGTACTGAGTGACGGAAAAGAAGTGGCGATCAAGAGGCTTTCAAGCTGTTCTGAGCAAGGCTCAGTGGAATTCACGAATGAGGTTCGGCTGCTAATGAAGCTTCAACATAAAAATCTTGTTCAGCTTTTGGGTTTTTGTGTTGAGGGAGAGGAAAAACTGCTTGTCTATGAATACCTGCCAAACAGCAGCCTAAATGTCTTCCTCTTTGATTCAGAGAAACGAGCGCAGCTTGATTGGAGCAGGCGTGTAAACATTATAAGTGGAATTGCAAGGGGAATTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3403 Pkinase_Tyr Stress-antifung

Protein Analysis

460

Amino Acids

51.59

Weight (kDa)

8.37

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 44 - 137 5.7e-20 Salt stress response/antifungal
Stress-antifung PF01657 156 - 249 1.8e-13 Salt stress response/antifungal
Pkinase PF00069 349 - 457 6.8e-14 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 352 - 458 1.1e-19 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g02731 FvH4_3g02732 FvH4_3g02732 FvH4_3g02732 FvH4_3g02751 FvH4_3g02752 FvH4_3g02770 FvH4_3g02770 FvH4_3g02770 FvH4_3g02771
malus_domestica MD10G1249000.v1.1 MD10G1249100.v1.1 MD10G1313300.v1.1 MD10G1313400.v1.1 MD10G1313500.v1.1 MD10G1313600.v1.1 MD10G1313800.v1.1
prunus_persica Prupe.4G027200_v2.0.a1 Prupe.6G140300_v2.0.a1 Prupe.6G140400_v2.0.a1 Prupe.6G140400_v2.0.a1
pyrus_communis pycom10g26540 pycom10g26560 pycom10g26580 pycom10g26600
rosa_chinensis RchiOBHm_Chr5g0004141 RchiOBHm_Chr5g0004151 RchiOBHm_Chr5g0004161
rosa_laevigata RLG00000031205 RLG00000031206 RLG00000031208 RLG00000031209 RLG00000031210
rosa_multiflora Rmu_co8256217.1_g000001 Rmu_co8309209.1_g000001 Rmu_sc0000547.1_g000034 Rmu_sc0004964.1_g000028 Rmu_sc0007868.1_g000001 Rmu_sc0010028.1_g000001 Rmu_sc0017938.1_g000001 Rmu_sc0027606.1_g000001 Rmu_sc0027892.1_g000001
rosa_roxburghii Rroxscaffold_1G00071540 Rroxscaffold_1G00071550 Rroxscaffold_1G00071610 Rroxscaffold_1G00071620 Rroxscaffold_1G00071640 Rroxscaffold_1G00071650 Rroxscaffold_1G00071790 Rroxscaffold_1G00071800 Rroxscaffold_1G00071810 Rroxscaffold_1G00071850 Rroxscaffold_1G00071860 Rroxscaffold_1G00071880 Rroxscaffold_1G00071890
rosa_rugosa Rorug04G0410500 Rorug04G0410500 Rorug04G0410500 Rorug04G0410600
rosa_samantha Rh5AG037700 Rh5AG037800 Rh5AG037900 Rh5AG038000 Rh5BG037000 Rh5BG037100 Rh5BG037200 Rh5CG040400 Rh5CG040500 Rh5CG040600 Rh5CG040700 Rh5DG036400 Rh5DG036600 Rh5DG036800
rosa_wichuraiana Rw5G003410 Rw5G003420 Rw5G003590 Rw5G003600 Rw5G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1352
Acc36I ACCTGC 1 cut(s) 1278
AccII CGCG 3 cut(s) 144, 329, 709
AciI CCGC 7 cut(s) 144, 240, 327, 456, 536, 709, 826
AclI AACGTT 1 cut(s) 471
AclWI GGATC 3 cut(s) 101, 107, 836
AcsI RAATTY 4 cut(s) 261, 1040, 1163, 1371
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 5 cut(s) 163, 230, 579, 740, 1098
AflIII ACRYGT 2 cut(s) 276, 943
AgsI TTSAA 8 cut(s) 207, 251, 433, 587, 971, 1053, 1137, 1199
AjuI GAANNNNNNNTTGG 4 cut(s) 401, 433, 1059, 1091
Alw21I GWGCWC 2 cut(s) 134, 620
Alw26I GTCTC 2 cut(s) 223, 618
Alw44I GTGCAC 1 cut(s) 616
AlwI GGATC 3 cut(s) 101, 107, 836
AoxI GGCC 2 cut(s) 268, 1079
ApaLI GTGCAC 1 cut(s) 616
ApeKI GCWGC 4 cut(s) 1028, 1183, 1281, 1321
ApoI RAATTY 4 cut(s) 261, 1040, 1163, 1371
AspLEI GCGC 4 cut(s) 331, 402, 709, 1321
AspS9I GGNCC 2 cut(s) 958, 1079
AsuC2I CCSGG 2 cut(s) 956, 983
AsuHPI GGTGA 2 cut(s) 70, 437
AvaII GGWCC 1 cut(s) 958
BaeGI GKGCMC 1 cut(s) 620
BbsI GAAGAC 2 cut(s) 678, 1285
Bbv12I GWGCWC 2 cut(s) 134, 620
BbvCI CCTCAGC 2 cut(s) 515, 990
BbvI GCAGC 4 cut(s) 1040, 1170, 1293, 1333
BccI CCATC 2 cut(s) 131, 816
BcnI CCSGG 2 cut(s) 956, 983
BcoDI GTCTC 2 cut(s) 223, 618
BfaI CTAG 3 cut(s) 33, 621, 860
BfuAI ACCTGC 1 cut(s) 1278
BisI GCNGC 6 cut(s) 537, 710, 1029, 1184, 1282, 1322
BlsI GCNGC 6 cut(s) 538, 711, 1030, 1185, 1283, 1323
Bme1390I CCNGG 2 cut(s) 956, 983
Bme18I GGWCC 1 cut(s) 958
BmgT120I GGNCC 2 cut(s) 958, 1079
BmiI GGNNCC 1 cut(s) 880
BmrFI CCNGG 2 cut(s) 956, 983
BmsI GCATC 2 cut(s) 327, 1025
BpiI GAAGAC 2 cut(s) 678, 1285
BplI GAGNNNNNCTC 2 cut(s) 1139, 1171
BpmI CTGGAG 2 cut(s) 591, 654
Bpu10I CCTNAGC 2 cut(s) 515, 990
BpuMI CCSGG 2 cut(s) 956, 983
BsaBI GATNNNNATC 2 cut(s) 111, 833
BsaXI ACNNNNNCTCC 4 cut(s) 374, 404, 1324, 1354
Bse1I ACTGG 1 cut(s) 574
Bse3DI GCAATG 1 cut(s) 197
Bse8I GATNNNNATC 2 cut(s) 111, 833
BseGI GGATG 1 cut(s) 827
BseJI GATNNNNATC 2 cut(s) 111, 833
BseMI GCAATG 1 cut(s) 197
BseMII CTCAG 7 cut(s) 147, 506, 725, 981, 1091, 1137, 1170
BseNI ACTGG 1 cut(s) 574
BseSI GKGCMC 1 cut(s) 620
BseXI GCAGC 4 cut(s) 1040, 1170, 1293, 1333
BsgI GTGCAG 1 cut(s) 632
Bsh1236I CGCG 3 cut(s) 144, 329, 709
BshFI GGCC 2 cut(s) 270, 1081
BsiHKAI GWGCWC 2 cut(s) 134, 620
BsiSI CCGG 4 cut(s) 243, 721, 956, 983
BslFI GGGAC 2 cut(s) 339, 824
BsmAI GTCTC 2 cut(s) 223, 618
BsmFI GGGAC 2 cut(s) 339, 824
BsnI GGCC 2 cut(s) 270, 1081
Bsp1286I GDGCHC 2 cut(s) 134, 620
Bsp1407I TGTACA 2 cut(s) 228, 577
Bsp143I GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
BspACI CCGC 7 cut(s) 144, 240, 327, 456, 536, 709, 826
BspANI GGCC 2 cut(s) 270, 1081
BspCNI CTCAG 7 cut(s) 146, 507, 726, 982, 1092, 1138, 1169
BspFNI CGCG 3 cut(s) 144, 329, 709
BspLI GGNNCC 1 cut(s) 880
BspMI ACCTGC 1 cut(s) 1278
BspPI GGATC 3 cut(s) 101, 107, 836
BsrDI GCAATG 1 cut(s) 197
BsrGI TGTACA 2 cut(s) 228, 577
BsrI ACTGG 1 cut(s) 574
BssMI GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
Bst4CI ACNGT 4 cut(s) 502, 644, 815, 844
BstAPI GCANNNNNTGC 2 cut(s) 545, 773
BstAUI TGTACA 2 cut(s) 228, 577
BstC8I GCNNGC 4 cut(s) 188, 534, 663, 1337
BstDEI CTNAG 7 cut(s) 133, 515, 734, 990, 1100, 1146, 1156
BstF5I GGATG 1 cut(s) 827
BstFNI CGCG 3 cut(s) 144, 329, 709
BstHHI GCGC 4 cut(s) 331, 402, 709, 1321
BstKTI GATC 6 cut(s) 13, 94, 109, 115, 831, 1125
BstMAI GTCTC 2 cut(s) 223, 618
BstMBI GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
BstMWI GCNNNNNNNGC 8 cut(s) 399, 520, 529, 545, 726, 773, 860, 1192
BstNSI RCATGY 3 cut(s) 280, 858, 947
BstSCI CCNGG 2 cut(s) 954, 981
BstSLI GKGCMC 1 cut(s) 620
BstUI CGCG 3 cut(s) 144, 329, 709
BstV1I GCAGC 4 cut(s) 1040, 1170, 1293, 1333
BstV2I GAAGAC 2 cut(s) 678, 1285
BsuRI GGCC 2 cut(s) 270, 1081
BtsCI GGATG 1 cut(s) 827
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 2 cut(s) 620, 1164
BveI ACCTGC 1 cut(s) 1278
Cac8I GCNNGC 4 cut(s) 188, 534, 663, 1337
CfoI GCGC 4 cut(s) 331, 402, 709, 1321
Cfr13I GGNCC 2 cut(s) 958, 1079
CpoI CGGWCCG 1 cut(s) 958
CseI GACGC 1 cut(s) 150
Csp6I GTAC 5 cut(s) 162, 229, 578, 739, 1097
CspI CGGWCCG 1 cut(s) 958
CviAII CATG 4 cut(s) 19, 277, 855, 944
CviQI GTAC 5 cut(s) 162, 229, 578, 739, 1097
DdeI CTNAG 7 cut(s) 133, 515, 734, 990, 1100, 1146, 1156
DpnI GATC 6 cut(s) 12, 93, 108, 114, 830, 1124
DpnII GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
EciI GGCGGA 2 cut(s) 229, 841
Eco47I GGWCC 1 cut(s) 958
Eco57I CTGAAG 1 cut(s) 534
EcoRI GAATTC 2 cut(s) 1163, 1371
FaeI CATG 4 cut(s) 22, 280, 858, 947
FaqI GGGAC 2 cut(s) 339, 824
FatI CATG 4 cut(s) 18, 276, 854, 943
Fnu4HI GCNGC 6 cut(s) 537, 710, 1029, 1184, 1282, 1322
FokI GGATG 1 cut(s) 834
Fsp4HI GCNGC 6 cut(s) 537, 710, 1029, 1184, 1282, 1322
FspBI CTAG 3 cut(s) 33, 621, 860
GlaI GCGC 4 cut(s) 330, 401, 708, 1320
GluI GCNGC 6 cut(s) 537, 710, 1029, 1184, 1282, 1322
GsuI CTGGAG 2 cut(s) 591, 654
HaeIII GGCC 2 cut(s) 270, 1081
HapII CCGG 4 cut(s) 243, 721, 956, 983
HgaI GACGC 1 cut(s) 150
HhaI GCGC 4 cut(s) 331, 402, 709, 1321
Hin1II CATG 4 cut(s) 22, 280, 858, 947
Hin6I GCGC 4 cut(s) 329, 400, 707, 1319
HinP1I GCGC 4 cut(s) 329, 400, 707, 1319
HindIII AAGCTT 1 cut(s) 1193
HinfI GANTC 4 cut(s) 368, 834, 1049, 1304
HpaII CCGG 4 cut(s) 243, 721, 956, 983
HphI GGTGA 2 cut(s) 70, 437
Hpy166II GTNNAC 4 cut(s) 618, 1087, 1097, 1345
Hpy188III TCNNGA 4 cut(s) 110, 633, 1126, 1168
Hpy8I GTNNAC 4 cut(s) 618, 1087, 1097, 1345
HpyAV CCTTC 3 cut(s) 213, 665, 1061
HpyCH4III ACNGT 4 cut(s) 502, 644, 815, 844
HpyCH4IV ACGT 4 cut(s) 164, 389, 471, 675
HpyCH4V TGCA 7 cut(s) 318, 613, 618, 767, 896, 1028, 1364
HpyF10VI GCNNNNNNNGC 8 cut(s) 399, 520, 529, 545, 726, 773, 860, 1192
HpyF3I CTNAG 7 cut(s) 133, 515, 734, 990, 1100, 1146, 1156
HpySE526I ACGT 4 cut(s) 164, 389, 471, 675
Hsp92II CATG 4 cut(s) 22, 280, 858, 947
HspAI GCGC 4 cut(s) 329, 400, 707, 1319
Kzo9I GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
LmnI GCTCC 2 cut(s) 520, 1332
Lsp1109I GCAGC 4 cut(s) 1040, 1170, 1293, 1333
LweI GCATC 2 cut(s) 327, 1025
MaeI CTAG 3 cut(s) 33, 621, 860
MaeII ACGT 4 cut(s) 164, 389, 471, 675
MaeIII GTNAC 4 cut(s) 292, 308, 644, 1103
MalI GATC 6 cut(s) 12, 93, 108, 114, 830, 1124
MboI GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
MboII GAAGA 3 cut(s) 599, 683, 1285
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 134, 620
MseI TTAA 2 cut(s) 24, 935
MspI CCGG 4 cut(s) 243, 721, 956, 983
MspR9I CCNGG 2 cut(s) 956, 983
MunI CAATTG 1 cut(s) 207
MvnI CGCG 3 cut(s) 144, 329, 709
MwoI GCNNNNNNNGC 8 cut(s) 399, 520, 529, 545, 726, 773, 860, 1192
NciI CCSGG 2 cut(s) 956, 983
NdeII GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
NlaIII CATG 4 cut(s) 22, 280, 858, 947
NlaIV GGNNCC 1 cut(s) 880
NmuCI GTSAC 3 cut(s) 308, 644, 1103
NspI RCATGY 3 cut(s) 280, 858, 947
PciI ACATGT 2 cut(s) 276, 943
PfeI GAWTC 4 cut(s) 368, 834, 1049, 1304
PfoI TCCNGGA 1 cut(s) 954
PkrI GCNGC 6 cut(s) 538, 711, 1030, 1185, 1283, 1323
PscI ACATGT 2 cut(s) 276, 943
PsiI TTATAA 1 cut(s) 1352
Psp1406I AACGTT 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 880
PspPI GGNCC 2 cut(s) 958, 1079
RsaI GTAC 5 cut(s) 163, 230, 579, 740, 1098
RsaNI GTAC 5 cut(s) 162, 229, 578, 739, 1097
Rsr2I CGGWCCG 1 cut(s) 958
RsrII CGGWCCG 1 cut(s) 958
SaqAI TTAA 2 cut(s) 24, 935
SatI GCNGC 6 cut(s) 537, 710, 1029, 1184, 1282, 1322
Sau3AI GATC 6 cut(s) 10, 91, 106, 112, 828, 1122
Sau96I GGNCC 2 cut(s) 958, 1079
ScrFI CCNGG 2 cut(s) 956, 983
SduI GDGCHC 2 cut(s) 134, 620
SfaNI GCATC 2 cut(s) 327, 1025
SinI GGWCC 1 cut(s) 958
SsiI CCGC 7 cut(s) 144, 240, 327, 456, 536, 709, 826
SspMI CTAG 3 cut(s) 33, 621, 860
StyD4I CCNGG 2 cut(s) 954, 981
TaaI ACNGT 4 cut(s) 502, 644, 815, 844
TaiI ACGT 4 cut(s) 167, 392, 474, 678
TaqI TCGA 1 cut(s) 153
TaqII GACCGA 1 cut(s) 975
TatI WGTACW 3 cut(s) 228, 577, 1096
TauI GCSGC 2 cut(s) 539, 712
TfiI GAWTC 4 cut(s) 368, 834, 1049, 1304
Tru1I TTAA 2 cut(s) 24, 935
Tru9I TTAA 2 cut(s) 24, 935
TscAI CASTG 2 cut(s) 620, 1164
TseFI GTSAC 3 cut(s) 308, 644, 1103
TseI GCWGC 4 cut(s) 1028, 1183, 1281, 1321
Tsp45I GTSAC 3 cut(s) 308, 644, 1103
TspDTI ATGAA 3 cut(s) 186, 1205, 1278
TspGWI ACGGA 1 cut(s) 1122
TspRI CASTG 2 cut(s) 620, 1164
VneI GTGCAC 1 cut(s) 616
VpaK11BI GGWCC 1 cut(s) 958
XapI RAATTY 4 cut(s) 261, 1040, 1163, 1371
XceI RCATGY 3 cut(s) 280, 858, 947
XcmI CCANNNNNNNNNTGG 1 cut(s) 871
XspI CTAG 3 cut(s) 33, 621, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.