Rroxscaffold_1G00071800

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000635
Physical Location & Seq
Reverse (-)
92932460 .. 92934095
1636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00071800.1

Sequence Viewer

Length: 972 bp
ATGGGAGGTTTCATGCCAGCTGCGCTACTCAATGAGAATTTCTTTGGCAGATTAAATGTATCGGGCAACGTTGTTCAAGTCAATAAACAGAGTATTTCAGAGCCAGAAAAGTTTAAGTCTGTTGTGAATCAAAAGCTGAGTGAACTCGCTAAGAAAGCGGCTTATAATGATTCTCTTCCAAAGATGTATGCTACTGGAGAAGCGCAATTTGGAGATAAAGTGATACATGCTCTTGTGCAGTGTACTACCGATTTATCTGAAGGTGACTGTGACAAATGCCTTCAGAGAGCCAGAGAAGACGTTTTAAGAGAATATTTCTTCGCGATGGGTGCAAGACTTTTCAGTCGTAGTTGTTTCCTGAGGTATGAGTTATACTCATTCTATGATGATGCAACACAAGCTTCTAAGGACAATAGTGTAATTTTAGATTTACCTACACTAGTCCATGAAAACCAATTTATAGGAAGGAACAACCTTAAAGCTCAGGAATACCGCAATGTCAGCTTTGCATCTATACGAGCAGCTACTTCCAACTTCTCTGAGTCCAATAAACTTGGGGAAGGTGGTTTTGGTCCTGTTTACAAGGGTATCATGAGTGATGGAAAGGAAGTGGCGATAAAGAGGCTTTCATGCTGTTCCGAGCAAGGTTCAGAGGAATTCACAACTGAGGTTCAACTGATAATGAATCTTCAACATAAAAATCTTGTCAGGCTTTTGGGTTATTGTGTAGATGGAGAGGAAAAGCTTCTTGTATATGAATACATGCCAAATAGCAGCCTAGATGTCATTCTTTTTGGTGGAATTCTCTATTTGCACGAGGACTCTCGACTTAGAATCATCCACAGAGACTTGAAAGCTAGCAATGTATTACTGGACAATGAGATGAACCCAAAGATCTCAGATTTTGGCATGGCCAGGATCTTTGCAGGAAGTGAAGGACAAGCTAATACTGCCATAATAGTTGGGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3403 Pkinase_Tyr Stress-antifung

Protein Analysis

323

Amino Acids

36.05

Weight (kDa)

5.54

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 30 - 124 3.3e-12 Salt stress response/antifungal
Pkinase PF00069 180 - 313 4.3e-25 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 182 - 308 6.4e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000635)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g02731 FvH4_3g02732 FvH4_3g02732 FvH4_3g02732 FvH4_3g02751 FvH4_3g02752 FvH4_3g02770 FvH4_3g02770 FvH4_3g02770 FvH4_3g02771
malus_domestica MD10G1249000.v1.1 MD10G1249100.v1.1 MD10G1313300.v1.1 MD10G1313400.v1.1 MD10G1313500.v1.1 MD10G1313600.v1.1 MD10G1313800.v1.1
prunus_persica Prupe.4G027200_v2.0.a1 Prupe.6G140300_v2.0.a1 Prupe.6G140400_v2.0.a1 Prupe.6G140400_v2.0.a1
pyrus_communis pycom10g26540 pycom10g26560 pycom10g26580 pycom10g26600
rosa_chinensis RchiOBHm_Chr5g0004141 RchiOBHm_Chr5g0004151 RchiOBHm_Chr5g0004161
rosa_laevigata RLG00000031205 RLG00000031206 RLG00000031208 RLG00000031209 RLG00000031210
rosa_multiflora Rmu_co8256217.1_g000001 Rmu_co8309209.1_g000001 Rmu_sc0000547.1_g000034 Rmu_sc0004964.1_g000028 Rmu_sc0007868.1_g000001 Rmu_sc0010028.1_g000001 Rmu_sc0017938.1_g000001 Rmu_sc0027606.1_g000001 Rmu_sc0027892.1_g000001
rosa_roxburghii Rroxscaffold_1G00071540 Rroxscaffold_1G00071550 Rroxscaffold_1G00071610 Rroxscaffold_1G00071620 Rroxscaffold_1G00071640 Rroxscaffold_1G00071650 Rroxscaffold_1G00071790 Rroxscaffold_1G00071800 Rroxscaffold_1G00071810 Rroxscaffold_1G00071850 Rroxscaffold_1G00071860 Rroxscaffold_1G00071880 Rroxscaffold_1G00071890
rosa_rugosa Rorug04G0410500 Rorug04G0410500 Rorug04G0410500 Rorug04G0410600
rosa_samantha Rh5AG037700 Rh5AG037800 Rh5AG037900 Rh5AG038000 Rh5BG037000 Rh5BG037100 Rh5BG037200 Rh5CG040400 Rh5CG040500 Rh5CG040600 Rh5CG040700 Rh5DG036400 Rh5DG036600 Rh5DG036800
rosa_wichuraiana Rw5G003410 Rw5G003420 Rw5G003590 Rw5G003600 Rw5G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 165
AasI GACNNNNNNGTC 1 cut(s) 342
AccII CGCG 1 cut(s) 323
AciI CCGC 2 cut(s) 158, 493
AclI AACGTT 1 cut(s) 69
AclWI GGATC 1 cut(s) 926
AcoI YGGCCR 1 cut(s) 912
AcsI RAATTY 3 cut(s) 37, 656, 801
AcuI CTGAAG 2 cut(s) 266, 279
AfaI GTAC 1 cut(s) 244
AgsI TTSAA 4 cut(s) 77, 674, 692, 853
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 1 cut(s) 914
AjuI GAANNNNNNNTTGG 4 cut(s) 192, 224, 552, 584
AluBI AGCT 9 cut(s) 20, 136, 401, 482, 504, 524, 745, 857, 944
AluI AGCT 9 cut(s) 20, 136, 401, 482, 504, 524, 745, 857, 944
Alw26I GTCTC 1 cut(s) 840
AlwI GGATC 1 cut(s) 926
AoxI GGCC 1 cut(s) 912
ApeKI GCWGC 3 cut(s) 20, 521, 774
ApoI RAATTY 3 cut(s) 37, 656, 801
Asp700I GAANNNNTTC 1 cut(s) 744
AspLEI GCGC 2 cut(s) 25, 205
AspS9I GGNCC 1 cut(s) 572
AsuHPI GGTGA 1 cut(s) 275
AsuNHI GCTAGC 1 cut(s) 857
AvaII GGWCC 1 cut(s) 572
AxyI CCTNAGG 1 cut(s) 359
BaeI ACNNNNGTAYC 2 cut(s) 571, 604
BalI TGGCCA 1 cut(s) 914
BauI CACGAG 1 cut(s) 815
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 7, 533, 786
BccI CCATC 3 cut(s) 319, 593, 725
BciT130I CCWGG 1 cut(s) 916
BcoDI GTCTC 1 cut(s) 840
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 3 cut(s) 440, 779, 858
BglII AGATCT 1 cut(s) 894
BisI GCNGC 4 cut(s) 21, 159, 522, 775
BlsI GCNGC 4 cut(s) 22, 160, 523, 776
Bme1390I CCNGG 1 cut(s) 916
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 572
BmrFI CCNGG 1 cut(s) 916
BmsI GCATC 2 cut(s) 379, 518
BmtI GCTAGC 1 cut(s) 861
BpiI GAAGAC 1 cut(s) 303
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 1 cut(s) 216
Bpu10I CCTNAGC 1 cut(s) 483
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bse1I ACTGG 2 cut(s) 199, 876
Bse21I CCTNAGG 1 cut(s) 359
Bse3DI GCAATG 2 cut(s) 502, 868
BseBI CCWGG 1 cut(s) 916
BseGI GGATG 1 cut(s) 837
BseMI GCAATG 2 cut(s) 502, 868
BseMII CTCAG 6 cut(s) 128, 350, 497, 531, 657, 912
BseNI ACTGG 2 cut(s) 199, 876
BseXI GCAGC 3 cut(s) 7, 533, 786
BsgI GTGCAG 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 323
BshFI GGCC 1 cut(s) 914
BsmAI GTCTC 1 cut(s) 840
BsnI GGCC 1 cut(s) 914
Bsp143I GATC 2 cut(s) 894, 918
Bsp68I TCGCGA 1 cut(s) 323
BspACI CCGC 2 cut(s) 158, 493
BspANI GGCC 1 cut(s) 914
BspCNI CTCAG 6 cut(s) 129, 351, 496, 532, 658, 911
BspFNI CGCG 1 cut(s) 323
BspHI TCATGA 1 cut(s) 591
BspOI GCTAGC 1 cut(s) 861
BspPI GGATC 1 cut(s) 926
BsrDI GCAATG 2 cut(s) 502, 868
BsrI ACTGG 2 cut(s) 199, 876
BssMI GATC 2 cut(s) 894, 918
BssSI CACGAG 1 cut(s) 815
Bst2BI CACGAG 1 cut(s) 815
Bst2UI CCWGG 1 cut(s) 916
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 18, 859
BstF5I GGATG 1 cut(s) 837
BstFNI CGCG 1 cut(s) 323
BstHHI GCGC 2 cut(s) 25, 205
BstKTI GATC 2 cut(s) 897, 921
BstMAI GTCTC 1 cut(s) 840
BstMBI GATC 2 cut(s) 894, 918
BstMWI GCNNNNNNNGC 6 cut(s) 22, 155, 329, 398, 501, 950
BstNI CCWGG 1 cut(s) 916
BstNSI RCATGY 2 cut(s) 230, 766
BstSCI CCNGG 1 cut(s) 914
BstUI CGCG 1 cut(s) 323
BstV1I GCAGC 3 cut(s) 7, 533, 786
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 2 cut(s) 894, 918
BstYI RGATCY 2 cut(s) 894, 918
Bsu36I CCTNAGG 1 cut(s) 359
BsuRI GGCC 1 cut(s) 914
BtgZI GCGATG 1 cut(s) 338
BtsCI GGATG 1 cut(s) 837
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 245
BtuMI TCGCGA 1 cut(s) 323
Cac8I GCNNGC 2 cut(s) 18, 859
CciI TCATGA 1 cut(s) 591
CfoI GCGC 2 cut(s) 25, 205
Cfr13I GGNCC 1 cut(s) 572
Csp6I GTAC 1 cut(s) 243
CviAII CATG 7 cut(s) 13, 227, 446, 592, 630, 763, 910
CviQI GTAC 1 cut(s) 243
DpnI GATC 2 cut(s) 896, 920
DpnII GATC 2 cut(s) 894, 918
DrdI GACNNNNNNGTC 1 cut(s) 342
DseDI GACNNNNNNGTC 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 912
Eam1104I CTCTTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 572
Eco57I CTGAAG 2 cut(s) 266, 279
Eco81I CCTNAGG 1 cut(s) 359
EcoRI GAATTC 2 cut(s) 656, 801
EcoRII CCWGG 1 cut(s) 914
FaeI CATG 7 cut(s) 16, 230, 449, 595, 633, 766, 913
FatI CATG 7 cut(s) 12, 226, 445, 591, 629, 762, 909
Fnu4HI GCNGC 4 cut(s) 21, 159, 522, 775
FokI GGATG 1 cut(s) 824
Fsp4HI GCNGC 4 cut(s) 21, 159, 522, 775
FspBI CTAG 3 cut(s) 440, 779, 858
GlaI GCGC 2 cut(s) 24, 204
GluI GCNGC 4 cut(s) 21, 159, 522, 775
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 1 cut(s) 914
HhaI GCGC 2 cut(s) 25, 205
Hin1II CATG 7 cut(s) 16, 230, 449, 595, 633, 766, 913
Hin6I GCGC 2 cut(s) 23, 203
HinP1I GCGC 2 cut(s) 23, 203
HindIII AAGCTT 2 cut(s) 399, 743
HinfI GANTC 6 cut(s) 127, 170, 542, 685, 821, 834
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 3 cut(s) 143, 243, 580
Hpy188I TCNGA 7 cut(s) 100, 259, 285, 541, 640, 652, 901
Hpy188III TCNNGA 5 cut(s) 322, 358, 485, 592, 825
Hpy8I GTNNAC 3 cut(s) 143, 243, 580
HpyAV CCTTC 5 cut(s) 254, 290, 459, 554, 929
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 2 cut(s) 69, 300
HpyCH4V TGCA 6 cut(s) 238, 332, 392, 509, 814, 926
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 155, 329, 398, 501, 950
HpySE526I ACGT 2 cut(s) 69, 300
Hsp92II CATG 7 cut(s) 16, 230, 449, 595, 633, 766, 913
HspAI GCGC 2 cut(s) 23, 203
Kzo9I GATC 2 cut(s) 894, 918
Lsp1109I GCAGC 3 cut(s) 7, 533, 786
LweI GCATC 2 cut(s) 379, 518
MaeI CTAG 3 cut(s) 440, 779, 858
MaeII ACGT 2 cut(s) 69, 300
MaeIII GTNAC 2 cut(s) 263, 269
MalI GATC 2 cut(s) 896, 920
MboI GATC 2 cut(s) 894, 918
MboII GAAGA 4 cut(s) 167, 308, 310, 680
MflI RGATCY 2 cut(s) 894, 918
MlsI TGGCCA 1 cut(s) 914
MluCI AATT 6 cut(s) 37, 206, 420, 455, 656, 801
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 2 cut(s) 551, 815
MmeI TCCRAC 1 cut(s) 555
MnlI CCTC 6 cut(s) 354, 615, 646, 661, 730, 811
Mox20I TGGCCA 1 cut(s) 914
MroXI GAANNNNTTC 1 cut(s) 744
MscI TGGCCA 1 cut(s) 914
MseI TTAA 4 cut(s) 53, 114, 305, 477
Msp20I TGGCCA 1 cut(s) 914
MspA1I CMGCKG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 916
MvaI CCWGG 1 cut(s) 916
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 6 cut(s) 22, 155, 329, 398, 501, 950
NdeII GATC 2 cut(s) 894, 918
NheI GCTAGC 1 cut(s) 857
NlaIII CATG 7 cut(s) 16, 230, 449, 595, 633, 766, 913
NmuCI GTSAC 2 cut(s) 263, 269
NruI TCGCGA 1 cut(s) 323
NspI RCATGY 2 cut(s) 230, 766
PagI TCATGA 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 744
PfeI GAWTC 4 cut(s) 127, 170, 685, 834
PkrI GCNGC 4 cut(s) 22, 160, 523, 776
PleI GAGTC 2 cut(s) 550, 815
PpsI GAGTC 2 cut(s) 550, 815
PsiI TTATAA 1 cut(s) 165
Psp1406I AACGTT 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 914
PspGI CCWGG 1 cut(s) 914
PspPI GGNCC 1 cut(s) 572
PsuI RGATCY 2 cut(s) 894, 918
PvuII CAGCTG 1 cut(s) 20
RruI TCGCGA 1 cut(s) 323
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
SaqAI TTAA 4 cut(s) 53, 114, 305, 477
SatI GCNGC 4 cut(s) 21, 159, 522, 775
Sau3AI GATC 2 cut(s) 894, 918
Sau96I GGNCC 1 cut(s) 572
SchI GAGTC 2 cut(s) 551, 815
ScrFI CCNGG 1 cut(s) 916
SfaNI GCATC 2 cut(s) 379, 518
SinI GGWCC 1 cut(s) 572
SpeI ACTAGT 1 cut(s) 439
Sse9I AATT 6 cut(s) 37, 206, 420, 455, 656, 801
SsiI CCGC 2 cut(s) 158, 493
SspI AATATT 1 cut(s) 314
SspMI CTAG 3 cut(s) 440, 779, 858
StyD4I CCNGG 1 cut(s) 914
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 2 cut(s) 72, 303
TaqI TCGA 1 cut(s) 826
TasI AATT 6 cut(s) 37, 206, 420, 455, 656, 801
TatI WGTACW 1 cut(s) 242
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 4 cut(s) 127, 170, 685, 834
Tru1I TTAA 4 cut(s) 53, 114, 305, 477
Tru9I TTAA 4 cut(s) 53, 114, 305, 477
TscAI CASTG 1 cut(s) 245
TseFI GTSAC 2 cut(s) 263, 269
TseI GCWGC 3 cut(s) 20, 521, 774
Tsp45I GTSAC 2 cut(s) 263, 269
TspDTI ATGAA 5 cut(s) 462, 618, 698, 771, 899
TspRI CASTG 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 572
XapI RAATTY 3 cut(s) 37, 656, 801
XceI RCATGY 2 cut(s) 230, 766
XmnI GAANNNNTTC 1 cut(s) 744
XspI CTAG 3 cut(s) 440, 779, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.