pycom11g03710

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001622
Physical Location & Seq
Forward (+)
2879255 .. 2882055
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1329 bp
ATGTCCCATTACGCTGGTTATGTGGAGGATGAATACGAAATGGAAGATGTAGATGATGATATGGATGATGAGTTTCGCGGTAGAGAAATGGGCGGCTCAGATTCTGATGTTGATGAATATGACTACTCGAATAGTAAAGTAGCTGATACAACTGCTGCTCAAGCAAGAAAAGGAAAAGACATCCAAGGTATTCCTTGGGATAGACTTAGCATTACTAGAGAGAAATACCGACAGACTAGGTTAGAACAATACAAGAATTATGAGAATATCCCTAATTCCGGAGAAGGATCAGGGAAGGATTGCAAAGATACAAAGAAAGGATCTGTATATTATCAGTTCAGACGAAATGCTAGATCAGTGAAATCAACCATTCTTCATTTTCAGTTGCGTAACTTGGTCTGGGCTACTTCCAAGCATGATGTCTACCTGATGTCACATTTTTCTGTCATTCATTGGTCTTCATTAAGTTGCACAAAGTCTGAGGTTCTCAATGTTTCTGGACATGTGGCACCATCTGAGAAACATCCTGGAAGTCTCTTGGAGGGATTTACGCAGACTCAAGTTAGTACTCTTGCTGTAAAAGATAAGTTGCTAGTTGCTGGAGGATTCCAGGGTGAACTTATTTGCAAGCATCTTGATCGGCCTGGAATCAGCTTTTGTTCAAGGACAACATATGACGAAAATGCCATAACAAATGCTGTTGAGATTTATGACAGTCCCAGCGGGGCAGTTCATTTTACAGCCTCTAATAATGACTGTGGAGTCAGAGATTTTGACATGGAGAAATTTCAGCTTACTAAGCATTTCCGTTACCCTTGGCCTGTGAATCATACTTCTCTGAGTCCTGATGGTAAACTTCTTGTGATCGTTGGAGACAATCCGGAAGGGATGTTGGTAAACTCTCAAACTGGAAAGACGGTGATGTCTTTAAGTGGGCACTTGGATTTCTCCTTTGCATCAGCATGGCATCCTGATGGTATGACCTTTGCTACTGGGAACCAGGACAAAACCTGCCGGATTTGGGATGTTCGAAACCTTTCCAAGTCGGTTGCTGTTTTGAAGGGGAACCTTGGAGCTATTCGGTCAATCCGGTATACTTCTGATGGTCAGTATATGATGATGGCAGAGCCCGCAGACTTTGTGCATGTCTATGATGTGAAAAGTGGGTACGAAAAAGAACAGGAAATTGATTTCTTTGGTGAGATATCTGGCATATCATTCAGTCCGGACACGGAGTCTCTTTTTATTGGAGTGTGGGATCGTACATACGGTAGTCTTCTTGAGTATGGCCGAAGTCGGAACTACTCTTACCTTGATTCTCTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2415 WD40

Protein Analysis

443

Amino Acids

49.81

Weight (kDa)

5.35

Isoelectric Point (pI)

29.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 192 - 389 3.8e-16 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 238 - 330 4.7e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 239 - 422 4.5e-16 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 240 - 392 1.6e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 241 - 347 9.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 246 - 373 5.3e-06 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 249 - 379 3.1e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 275 - 421 7.5e-11 WDR3 first beta-propeller domain
WDR55 PF24796 277 - 389 1.1e-07 WDR55
WD40_CDC20-Fz PF24807 278 - 414 5e-08 CDC20/Fizzy WD40 domain
WD40 PF00400 304 - 342 1.1e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 322 - 421 3.8e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 761, 922
Acc36I ACCTGC 1 cut(s) 1019
AccB1I GGYRCC 1 cut(s) 508
AccI GTMKAC 2 cut(s) 423, 1094
AccII CGCG 1 cut(s) 78
AccIII TCCGGA 3 cut(s) 278, 880, 1225
AciI CCGC 4 cut(s) 78, 93, 723, 1131
AclWI GGATC 3 cut(s) 295, 328, 1266
AcoI YGGCCR 1 cut(s) 1288
AcsI RAATTY 1 cut(s) 785
AfaI GTAC 3 cut(s) 568, 1169, 1264
AfiI CCNNNNNNNGG 2 cut(s) 278, 1021
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 2 cut(s) 663, 1060
AhdI GACNNNNNGTC 1 cut(s) 1234
AjnI CCWGG 4 cut(s) 526, 609, 643, 999
AluBI AGCT 4 cut(s) 143, 654, 793, 1076
AluI AGCT 4 cut(s) 143, 654, 793, 1076
Alw26I GTCTC 3 cut(s) 539, 867, 1242
AlwI GGATC 3 cut(s) 295, 328, 1266
AlwNI CAGNNNCTG 1 cut(s) 104
Aor13HI TCCGGA 3 cut(s) 278, 880, 1225
AoxI GGCC 3 cut(s) 641, 818, 1288
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 785
Asp700I GAANNNNTTC 1 cut(s) 1036
AsuHPI GGTGA 3 cut(s) 626, 931, 1211
AsuII TTCGAA 1 cut(s) 1030
BaeGI GKGCMC 1 cut(s) 939
BanI GGYRCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 1131
BbsI GAAGAC 2 cut(s) 450, 1268
BbvI GCAGC 1 cut(s) 142
BccI CCATC 5 cut(s) 520, 842, 968, 1097, 1114
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BciT130I CCWGG 4 cut(s) 528, 611, 645, 1001
BcoDI GTCTC 3 cut(s) 539, 867, 1242
BfaI CTAG 4 cut(s) 216, 237, 351, 593
BfuAI ACCTGC 1 cut(s) 1019
BisI GCNGC 2 cut(s) 94, 156
BlsI GCNGC 2 cut(s) 95, 157
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 4 cut(s) 528, 611, 645, 1001
BmeRI GACNNNNNGTC 1 cut(s) 1234
BmiI GGNNCC 3 cut(s) 510, 998, 1067
BmrFI CCNGG 4 cut(s) 528, 611, 645, 1001
BmrI ACTGGG 1 cut(s) 1002
BmsI GCATC 3 cut(s) 640, 965, 976
BmuI ACTGGG 1 cut(s) 1002
BpiI GAAGAC 2 cut(s) 450, 1268
BpmI CTGGAG 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 1030
BpuEI CTTGAG 3 cut(s) 144, 543, 1301
BsaJI CCNNGG 5 cut(s) 184, 194, 610, 815, 1069
BsaWI WCCGGW 4 cut(s) 278, 880, 1089, 1225
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 1021
Bse1I ACTGG 2 cut(s) 913, 997
BseAI TCCGGA 3 cut(s) 278, 880, 1225
BseBI CCWGG 4 cut(s) 528, 611, 645, 1001
BseDI CCNNGG 5 cut(s) 184, 194, 610, 815, 1069
BseGI GGATG 7 cut(s) 34, 70, 180, 523, 894, 967, 1030
BseLI CCNNNNNNNGG 2 cut(s) 278, 1021
BseMII CTCAG 4 cut(s) 111, 471, 507, 830
BseNI ACTGG 2 cut(s) 913, 997
BseSI GKGCMC 1 cut(s) 939
BseXI GCAGC 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 719
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 3 cut(s) 643, 820, 1290
BshNI GGYRCC 1 cut(s) 508
BsiSI CCGG 5 cut(s) 279, 881, 1015, 1090, 1226
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 2 cut(s) 278, 1021
BsmAI GTCTC 3 cut(s) 539, 867, 1242
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 3 cut(s) 643, 820, 1290
Bsp119I TTCGAA 1 cut(s) 1030
Bsp1286I GDGCHC 2 cut(s) 939, 1131
Bsp13I TCCGGA 3 cut(s) 278, 880, 1225
Bsp143I GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
BspACI CCGC 4 cut(s) 78, 93, 723, 1131
BspANI GGCC 3 cut(s) 643, 820, 1290
BspCNI CTCAG 4 cut(s) 110, 472, 508, 831
BspEI TCCGGA 3 cut(s) 278, 880, 1225
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 3 cut(s) 510, 998, 1067
BspMI ACCTGC 1 cut(s) 1019
BspPI GGATC 3 cut(s) 295, 328, 1266
BspT104I TTCGAA 1 cut(s) 1030
BspT107I GGYRCC 1 cut(s) 508
BsrI ACTGG 2 cut(s) 913, 997
BssECI CCNNGG 5 cut(s) 184, 194, 610, 815, 1069
BssMI GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
BssNAI GTATAC 1 cut(s) 1095
BssT1I CCWWGG 4 cut(s) 184, 194, 815, 1069
Bst1107I GTATAC 1 cut(s) 1095
Bst2UI CCWGG 4 cut(s) 528, 611, 645, 1001
Bst4CI ACNGT 4 cut(s) 716, 758, 919, 1271
BstBI TTCGAA 1 cut(s) 1030
BstC8I GCNNGC 2 cut(s) 629, 1131
BstDEI CTNAG 6 cut(s) 97, 206, 480, 516, 798, 839
BstF5I GGATG 7 cut(s) 34, 70, 180, 523, 894, 967, 1030
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 6 cut(s) 290, 323, 356, 640, 867, 1261
BstMAI GTCTC 3 cut(s) 539, 867, 1242
BstMBI GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
BstMWI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
BstNI CCWGG 4 cut(s) 528, 611, 645, 1001
BstNSI RCATGY 2 cut(s) 506, 1148
BstSCI CCNGG 4 cut(s) 526, 609, 643, 999
BstSLI GKGCMC 1 cut(s) 939
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 2 cut(s) 450, 1268
BstX2I RGATCY 1 cut(s) 320
BstXI CCANNNNNNTGG 1 cut(s) 14
BstYI RGATCY 1 cut(s) 320
BstZ17I GTATAC 1 cut(s) 1095
BsuRI GGCC 3 cut(s) 643, 820, 1290
BtsCI GGATG 7 cut(s) 34, 70, 180, 523, 894, 967, 1030
BtsIMutI CAGTG 1 cut(s) 363
BveI ACCTGC 1 cut(s) 1019
Cac8I GCNNGC 2 cut(s) 629, 1131
CaiI CAGNNNCTG 1 cut(s) 104
Csp6I GTAC 3 cut(s) 567, 1168, 1263
CviAII CATG 5 cut(s) 416, 503, 778, 963, 1145
CviQI GTAC 3 cut(s) 567, 1168, 1263
DdeI CTNAG 6 cut(s) 97, 206, 480, 516, 798, 839
DpnI GATC 6 cut(s) 289, 322, 355, 639, 866, 1260
DpnII GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
DrdI GACNNNNNNGTC 2 cut(s) 761, 922
DriI GACNNNNNGTC 1 cut(s) 1234
DseDI GACNNNNNNGTC 2 cut(s) 761, 922
EaeI YGGCCR 1 cut(s) 1288
Eam1105I GACNNNNNGTC 1 cut(s) 1234
Eco130I CCWWGG 4 cut(s) 184, 194, 815, 1069
Eco24I GRGCYC 1 cut(s) 1131
Eco32I GATATC 1 cut(s) 1206
EcoRII CCWGG 4 cut(s) 526, 609, 643, 999
EcoRV GATATC 1 cut(s) 1206
EcoT14I CCWWGG 4 cut(s) 184, 194, 815, 1069
EcoT38I GRGCYC 1 cut(s) 1131
ErhI CCWWGG 4 cut(s) 184, 194, 815, 1069
FaeI CATG 5 cut(s) 419, 506, 781, 966, 1148
FaqI GGGAC 1 cut(s) 702
FatI CATG 5 cut(s) 415, 502, 777, 962, 1144
FauI CCCGC 2 cut(s) 716, 1138
FauNDI CATATG 1 cut(s) 673
FblI GTMKAC 2 cut(s) 423, 1094
Fnu4HI GCNGC 2 cut(s) 94, 156
FokI GGATG 7 cut(s) 41, 77, 167, 510, 901, 954, 1037
FriOI GRGCYC 1 cut(s) 1131
Fsp4HI GCNGC 2 cut(s) 94, 156
FspBI CTAG 4 cut(s) 216, 237, 351, 593
GluI GCNGC 2 cut(s) 94, 156
GsaI CCCAGC 1 cut(s) 723
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 3 cut(s) 643, 820, 1290
HapII CCGG 5 cut(s) 279, 881, 1015, 1090, 1226
Hin1II CATG 5 cut(s) 419, 506, 781, 966, 1148
HinfI GANTC 9 cut(s) 101, 556, 606, 648, 762, 826, 841, 1235, 1316
HpaII CCGG 5 cut(s) 279, 881, 1015, 1090, 1226
HphI GGTGA 3 cut(s) 626, 931, 1211
Hpy166II GTNNAC 5 cut(s) 424, 617, 854, 898, 1095
Hpy188I TCNGA 9 cut(s) 100, 106, 341, 481, 517, 767, 840, 1102, 1299
Hpy188III TCNNGA 8 cut(s) 279, 498, 635, 845, 881, 971, 1226, 1280
Hpy8I GTNNAC 5 cut(s) 424, 617, 854, 898, 1095
HpyAV CCTTC 4 cut(s) 278, 289, 878, 1054
HpyCH4III ACNGT 4 cut(s) 716, 758, 919, 1271
HpyCH4V TGCA 5 cut(s) 303, 471, 627, 956, 1144
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
HpyF3I CTNAG 6 cut(s) 97, 206, 480, 516, 798, 839
Hsp92II CATG 5 cut(s) 419, 506, 781, 966, 1148
Kpn2I TCCGGA 3 cut(s) 278, 880, 1225
Kzo9I GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
LmnI GCTCC 1 cut(s) 1073
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 3 cut(s) 640, 965, 976
MaeI CTAG 4 cut(s) 216, 237, 351, 593
MaeIII GTNAC 3 cut(s) 389, 432, 809
MalI GATC 6 cut(s) 289, 322, 355, 639, 866, 1260
MboI GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
MboII GAAGA 4 cut(s) 56, 365, 450, 1268
MflI RGATCY 1 cut(s) 320
MhlI GDGCHC 2 cut(s) 939, 1131
MluCI AATT 4 cut(s) 256, 274, 785, 1185
MlyI GAGTC 4 cut(s) 550, 771, 850, 1244
MmeI TCCRAC 2 cut(s) 850, 1277
MnlI CCTC 5 cut(s) 19, 475, 535, 596, 754
MroI TCCGGA 3 cut(s) 278, 880, 1225
MroXI GAANNNNTTC 1 cut(s) 1036
MseI TTAA 2 cut(s) 464, 929
MslI CAYNNNNRTG 3 cut(s) 961, 972, 1149
MspA1I CMGCKG 1 cut(s) 723
MspI CCGG 5 cut(s) 279, 881, 1015, 1090, 1226
MspR9I CCNGG 4 cut(s) 528, 611, 645, 1001
MvaI CCWGG 4 cut(s) 528, 611, 645, 1001
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 161, 799, 1130
NdeI CATATG 1 cut(s) 673
NdeII GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
NlaIII CATG 5 cut(s) 419, 506, 781, 966, 1148
NlaIV GGNNCC 3 cut(s) 510, 998, 1067
NmuCI GTSAC 1 cut(s) 432
NspI RCATGY 2 cut(s) 506, 1148
NspV TTCGAA 1 cut(s) 1030
PciI ACATGT 1 cut(s) 502
PdmI GAANNNNTTC 1 cut(s) 1036
PfeI GAWTC 5 cut(s) 101, 606, 648, 826, 1316
PfoI TCCNGGA 1 cut(s) 526
PkrI GCNGC 2 cut(s) 95, 157
PleI GAGTC 4 cut(s) 550, 770, 849, 1243
PpsI GAGTC 4 cut(s) 550, 770, 849, 1243
PscI ACATGT 1 cut(s) 502
Psp6I CCWGG 4 cut(s) 526, 609, 643, 999
PspFI CCCAGC 1 cut(s) 719
PspGI CCWGG 4 cut(s) 526, 609, 643, 999
PspN4I GGNNCC 3 cut(s) 510, 998, 1067
PsrI GAACNNNNNNTAC 2 cut(s) 1292, 1324
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 320
RsaI GTAC 3 cut(s) 568, 1169, 1264
RsaNI GTAC 3 cut(s) 567, 1168, 1263
RseI CAYNNNNRTG 3 cut(s) 961, 972, 1149
SaqAI TTAA 2 cut(s) 464, 929
SatI GCNGC 2 cut(s) 94, 156
Sau3AI GATC 6 cut(s) 287, 320, 353, 637, 864, 1258
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 4 cut(s) 550, 771, 850, 1244
ScrFI CCNGG 4 cut(s) 528, 611, 645, 1001
SduI GDGCHC 2 cut(s) 939, 1131
SfaNI GCATC 3 cut(s) 640, 965, 976
SfuI TTCGAA 1 cut(s) 1030
SmiMI CAYNNNNRTG 3 cut(s) 961, 972, 1149
SmlI CTYRAG 3 cut(s) 159, 558, 1280
SmoI CTYRAG 3 cut(s) 159, 558, 1280
Sse9I AATT 4 cut(s) 256, 274, 785, 1185
SsiI CCGC 4 cut(s) 78, 93, 723, 1131
SspMI CTAG 4 cut(s) 216, 237, 351, 593
StyD4I CCNGG 4 cut(s) 526, 609, 643, 999
StyI CCWWGG 4 cut(s) 184, 194, 815, 1069
TaaI ACNGT 4 cut(s) 716, 758, 919, 1271
TaqI TCGA 2 cut(s) 128, 1030
TaqII GACCGA 1 cut(s) 1071
TasI AATT 4 cut(s) 256, 274, 785, 1185
TatI WGTACW 1 cut(s) 566
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 5 cut(s) 101, 606, 648, 826, 1316
Tru1I TTAA 2 cut(s) 464, 929
Tru9I TTAA 2 cut(s) 464, 929
TscAI CASTG 1 cut(s) 363
TseFI GTSAC 1 cut(s) 432
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 1 cut(s) 432
TspDTI ATGAA 6 cut(s) 45, 129, 365, 440, 450, 722
TspGWI ACGGA 2 cut(s) 797, 1247
TspRI CASTG 1 cut(s) 363
XapI RAATTY 1 cut(s) 785
XceI RCATGY 2 cut(s) 506, 1148
XmiI GTMKAC 2 cut(s) 423, 1094
XmnI GAANNNNTTC 1 cut(s) 1036
XspI CTAG 4 cut(s) 216, 237, 351, 593
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.