pycom16g10580

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001652
Physical Location & Seq
Reverse (-)
7251810 .. 7254139
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGGCGGCTCCGTTGACCACCCTGTCAAATGTTGGTTCGCTTCCGGCTTCTCGCAGCGTGTCTCAGATCAGAGTTTTGACCCCTACCTGTTCTTCGGTCACAATCCCATCGTCGTCTAACAAAACGGTGTCGTTGTCCTCCAGCTTATTCTCCAGGTCGTCGCGCTTGTCCTCTCTGACATTCTCTTCGAACCCTAGTTCCCGGAAAAGGACCAGTCTTGCTGGCTCAGTCCGCGCCAGTGCTGAGGAAGCAACTTTGCAATCAAAAGTGACCCATAAGGTGTATTTTGATATTAGCATTGGGAACCCAGTTGGGAAGCTTGCTGGAAGGATTGTGATTGGATTGTATGGTGATGATGTGCCTCAAACCGTGGAGAATTTCCGTGCGCTTTGCACAGGTGATAAAGGCTTCGGGTTCAAGGGGTCCGCATTCCATCGTGTGATCAAGGATTTCATGATTCAAGGAGGGGACTTCGATAAAGGCAATGGCACTGGAGGTAAAAGCATTTACGGACGTACTTTCAAGGATGAGAACTTCAAGTTATCTCATACTGGACCAGGAGTTGTTAGCATGGCCAATGCAGGGCCCAATACCAATGGGAGCCAGTTCTTCATATGCACGGTCAAGACACCGTGGCTAGACAACAGGCATGTCGTTTTTGGGCAAGTGGTGGAAGGCCTGGACGTTGTTAAACTAATCGAGTCTCAAGAGACAGACAGAGGTGATCGTCCTACAAAGAAGGTGGTGATTGCCGACTGCGGAGAGCTGCCGGTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pro_isomerase

Protein Analysis

260

Amino Acids

27.53

Weight (kDa)

9.54

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 102 - 254 3.5e-51 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccII CGCG 2 cut(s) 163, 234
AciI CCGC 4 cut(s) 5, 232, 426, 759
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 1 cut(s) 376
AfaI GTAC 1 cut(s) 517
AfiI CCNNNNNNNGG 2 cut(s) 207, 582
AgsI TTSAA 4 cut(s) 418, 461, 523, 538
AjnI CCWGG 3 cut(s) 152, 556, 678
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
AluBI AGCT 3 cut(s) 144, 319, 766
AluI AGCT 3 cut(s) 144, 319, 766
Alw26I GTCTC 3 cut(s) 66, 704, 708
AoxI GGCC 4 cut(s) 573, 584, 676, 774
ApaI GGGCCC 1 cut(s) 588
ApeKI GCWGC 2 cut(s) 54, 766
ApoI RAATTY 1 cut(s) 376
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AspLEI GCGC 3 cut(s) 165, 236, 388
AspS9I GGNCC 5 cut(s) 210, 423, 554, 584, 585
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 4 cut(s) 362, 410, 734, 757
AsuII TTCGAA 1 cut(s) 188
AvaII GGWCC 3 cut(s) 210, 423, 554
BaeGI GKGCMC 1 cut(s) 588
BalI TGGCCA 1 cut(s) 575
BanII GRGCYC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 2 cut(s) 66, 753
BccI CCATC 2 cut(s) 115, 441
BciT130I CCWGG 3 cut(s) 154, 558, 680
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 3 cut(s) 66, 704, 708
BfaI CTAG 2 cut(s) 195, 638
BisI GCNGC 3 cut(s) 6, 55, 767
BlsI GCNGC 3 cut(s) 7, 56, 768
Bme1390I CCNGG 4 cut(s) 154, 202, 558, 680
Bme18I GGWCC 3 cut(s) 210, 423, 554
BmgT120I GGNCC 5 cut(s) 210, 423, 554, 584, 585
BmiI GGNNCC 5 cut(s) 9, 305, 424, 586, 602
BmrFI CCNGG 4 cut(s) 154, 202, 558, 680
BmrI ACTGGG 1 cut(s) 302
BmuI ACTGGG 1 cut(s) 302
BpmI CTGGAG 3 cut(s) 124, 136, 513
Bpu10I CCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 690
BpuMI CCSGG 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 369, 632
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 582
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 6 cut(s) 213, 237, 308, 496, 556, 604
Bse3DI GCAATG 1 cut(s) 490
BseBI CCWGG 3 cut(s) 154, 558, 680
BseDI CCNNGG 2 cut(s) 369, 632
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 2 cut(s) 207, 582
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 3 cut(s) 77, 234, 240
BseNI ACTGG 6 cut(s) 213, 237, 308, 496, 556, 604
BseSI GKGCMC 1 cut(s) 588
BseXI GCAGC 2 cut(s) 66, 753
Bsh1236I CGCG 2 cut(s) 163, 234
BshFI GGCC 4 cut(s) 575, 586, 678, 776
BsiSI CCGG 3 cut(s) 44, 202, 770
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 2 cut(s) 207, 582
BsmAI GTCTC 3 cut(s) 66, 704, 708
BsmFI GGGAC 1 cut(s) 482
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 4 cut(s) 575, 586, 678, 776
Bsp119I TTCGAA 1 cut(s) 188
Bsp120I GGGCCC 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 588
Bsp143I GATC 3 cut(s) 66, 441, 724
BspACI CCGC 4 cut(s) 5, 232, 426, 759
BspANI GGCC 4 cut(s) 575, 586, 678, 776
BspCNI CTCAG 3 cut(s) 76, 235, 239
BspFNI CGCG 2 cut(s) 163, 234
BspHI TCATGA 1 cut(s) 453
BspLI GGNNCC 5 cut(s) 9, 305, 424, 586, 602
BspT104I TTCGAA 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 490
BsrFI RCCGGY 1 cut(s) 769
BsrI ACTGG 6 cut(s) 213, 237, 308, 496, 556, 604
BssAI RCCGGY 1 cut(s) 769
BssECI CCNNGG 2 cut(s) 369, 632
BssMI GATC 3 cut(s) 66, 441, 724
Bst2UI CCWGG 3 cut(s) 154, 558, 680
Bst4CI ACNGT 4 cut(s) 127, 370, 622, 633
Bst6I CTCTTC 1 cut(s) 190
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 223, 321
BstDEI CTNAG 3 cut(s) 63, 226, 243
BstDSI CCRYGG 2 cut(s) 369, 632
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 2 cut(s) 163, 234
BstHHI GCGC 3 cut(s) 165, 236, 388
BstKTI GATC 3 cut(s) 69, 444, 727
BstMAI GTCTC 3 cut(s) 66, 704, 708
BstMBI GATC 3 cut(s) 66, 441, 724
BstMWI GCNNNNNNNGC 2 cut(s) 231, 248
BstNI CCWGG 3 cut(s) 154, 558, 680
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 4 cut(s) 152, 200, 556, 678
BstSLI GKGCMC 1 cut(s) 588
BstUI CGCG 2 cut(s) 163, 234
BstV1I GCAGC 2 cut(s) 66, 753
BsuRI GGCC 4 cut(s) 575, 586, 678, 776
BtgI CCRYGG 2 cut(s) 369, 632
BtsCI GGATG 1 cut(s) 532
BtsIMutI CAGTG 2 cut(s) 244, 489
Cac8I GCNNGC 2 cut(s) 223, 321
CciI TCATGA 1 cut(s) 453
CfoI GCGC 3 cut(s) 165, 236, 388
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 5 cut(s) 210, 423, 554, 584, 585
Csp6I GTAC 1 cut(s) 516
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 3 cut(s) 454, 571, 650
CviQI GTAC 1 cut(s) 516
DdeI CTNAG 3 cut(s) 63, 226, 243
DpnI GATC 3 cut(s) 68, 443, 726
DpnII GATC 3 cut(s) 66, 441, 724
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco147I AGGCCT 1 cut(s) 678
Eco24I GRGCYC 1 cut(s) 588
Eco47I GGWCC 3 cut(s) 210, 423, 554
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 3 cut(s) 152, 556, 678
EcoT38I GRGCYC 1 cut(s) 588
FaeI CATG 3 cut(s) 457, 574, 653
FaiI YATR 8 cut(s) 276, 348, 455, 549, 572, 614, 616, 651
FaqI GGGAC 1 cut(s) 482
FatI CATG 3 cut(s) 453, 570, 649
FauNDI CATATG 1 cut(s) 614
FbaI TGATCA 1 cut(s) 441
Fnu4HI GCNGC 3 cut(s) 6, 55, 767
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 3 cut(s) 6, 55, 767
FspBI CTAG 2 cut(s) 195, 638
GlaI GCGC 3 cut(s) 164, 235, 387
GluI GCNGC 3 cut(s) 6, 55, 767
GsuI CTGGAG 3 cut(s) 124, 136, 513
HaeIII GGCC 4 cut(s) 575, 586, 678, 776
HapII CCGG 3 cut(s) 44, 202, 770
HhaI GCGC 3 cut(s) 165, 236, 388
Hin1II CATG 3 cut(s) 457, 574, 653
Hin6I GCGC 3 cut(s) 163, 234, 386
HinP1I GCGC 3 cut(s) 163, 234, 386
HincII GTYRAC 1 cut(s) 15
HindII GTYRAC 1 cut(s) 15
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 2 cut(s) 457, 701
HpaII CCGG 3 cut(s) 44, 202, 770
HphI GGTGA 4 cut(s) 362, 410, 734, 757
Hpy166II GTNNAC 1 cut(s) 15
Hpy188I TCNGA 3 cut(s) 66, 71, 177
Hpy188III TCNNGA 3 cut(s) 454, 625, 707
Hpy8I GTNNAC 1 cut(s) 15
Hpy99I CGWCG 2 cut(s) 115, 163
HpyAV CCTTC 3 cut(s) 321, 668, 733
HpyCH4III ACNGT 4 cut(s) 127, 370, 622, 633
HpyCH4IV ACGT 2 cut(s) 514, 684
HpyCH4V TGCA 4 cut(s) 259, 393, 581, 618
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 248
HpyF3I CTNAG 3 cut(s) 63, 226, 243
HpySE526I ACGT 2 cut(s) 514, 684
Hsp92II CATG 3 cut(s) 457, 574, 653
HspAI GCGC 3 cut(s) 163, 234, 386
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 3 cut(s) 66, 441, 724
LmnI GCTCC 2 cut(s) 13, 600
Lsp1109I GCAGC 2 cut(s) 66, 753
MaeI CTAG 2 cut(s) 195, 638
MaeII ACGT 2 cut(s) 514, 684
MaeIII GTNAC 2 cut(s) 97, 268
MalI GATC 3 cut(s) 68, 443, 726
MboI GATC 3 cut(s) 66, 441, 724
MboII GAAGA 3 cut(s) 84, 177, 601
MhlI GDGCHC 1 cut(s) 588
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 1 cut(s) 376
MluNI TGGCCA 1 cut(s) 575
MlyI GAGTC 1 cut(s) 710
MnlI CCTC 7 cut(s) 148, 181, 238, 372, 458, 488, 713
Mox20I TGGCCA 1 cut(s) 575
MscI TGGCCA 1 cut(s) 575
MseI TTAA 1 cut(s) 690
Msp20I TGGCCA 1 cut(s) 575
MspI CCGG 3 cut(s) 44, 202, 770
MspR9I CCNGG 4 cut(s) 154, 202, 558, 680
Mva1269I GAATGC 1 cut(s) 428
MvaI CCWGG 3 cut(s) 154, 558, 680
MvnI CGCG 2 cut(s) 163, 234
MwoI GCNNNNNNNGC 2 cut(s) 231, 248
NciI CCSGG 1 cut(s) 202
NdeI CATATG 1 cut(s) 614
NdeII GATC 3 cut(s) 66, 441, 724
NlaIII CATG 3 cut(s) 457, 574, 653
NlaIV GGNNCC 5 cut(s) 9, 305, 424, 586, 602
NmuCI GTSAC 2 cut(s) 97, 268
NspI RCATGY 1 cut(s) 653
NspV TTCGAA 1 cut(s) 188
PagI TCATGA 1 cut(s) 453
PceI AGGCCT 1 cut(s) 678
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 457
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 3 cut(s) 7, 56, 768
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
Psp6I CCWGG 3 cut(s) 152, 556, 678
PspGI CCWGG 3 cut(s) 152, 556, 678
PspN4I GGNNCC 5 cut(s) 9, 305, 424, 586, 602
PspOMI GGGCCC 1 cut(s) 584
PspPI GGNCC 5 cut(s) 210, 423, 554, 584, 585
RsaI GTAC 1 cut(s) 517
RsaNI GTAC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 3 cut(s) 6, 55, 767
Sau3AI GATC 3 cut(s) 66, 441, 724
Sau96I GGNCC 5 cut(s) 210, 423, 554, 584, 585
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 4 cut(s) 154, 202, 558, 680
SduI GDGCHC 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 188
SinI GGWCC 3 cut(s) 210, 423, 554
SmlI CTYRAG 1 cut(s) 705
SmoI CTYRAG 1 cut(s) 705
Sse9I AATT 1 cut(s) 376
SseBI AGGCCT 1 cut(s) 678
SsiI CCGC 4 cut(s) 5, 232, 426, 759
SspMI CTAG 2 cut(s) 195, 638
StuI AGGCCT 1 cut(s) 678
StyD4I CCNGG 4 cut(s) 152, 200, 556, 678
TaaI ACNGT 4 cut(s) 127, 370, 622, 633
TaiI ACGT 2 cut(s) 517, 687
TaqI TCGA 3 cut(s) 188, 474, 699
TaqII GACCGA 1 cut(s) 85
TasI AATT 1 cut(s) 376
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 2 cut(s) 244, 496
TseFI GTSAC 2 cut(s) 97, 268
TseI GCWGC 2 cut(s) 54, 766
Tsp45I GTSAC 2 cut(s) 97, 268
TspDTI ATGAA 2 cut(s) 442, 601
TspGWI ACGGA 2 cut(s) 371, 525
TspRI CASTG 2 cut(s) 244, 496
VpaK11BI GGWCC 3 cut(s) 210, 423, 554
XapI RAATTY 1 cut(s) 376
XceI RCATGY 1 cut(s) 653
XspI CTAG 2 cut(s) 195, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.