Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001363

Physical Location & Seq

Genomic Coordinates Reverse (-)

11227135 .. 11228982

Gene Structure 1848 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGTACTTCAAATGCGGCGATGAGGTTGATGCACGCAAGGTGTTTGATAAAATGTTTGTGAGGAATTTGTACTCGTGGAACAATATGTTATCTGGGCATGTGAAGATGAGGAATCTGAAGGAGGCCCGGAGCTTGTTTGATAAAATGCCTGAGAGGGATGTTGTGTCCTGGAACACCATGGTGATTGGTTATGCCCAGAGTGGGGATTGTAATGAGGCTTTGAGGTTTTATAGAGAGTTGCGGAGGTCGCCGGTTGGGTTGAATGAGTTTAGTTTTGCAGGTGTTTTGACTGTGTGTGTGAAGATGAGGGAATGGAAGCTTATTAGGCAGGCTCATGGGCAGGTTTTGGTTGCTGGGTTTTTGTCGAATGTGGTGCTTTCGAGTTCGGTGGTGGATGCTTATGCCAAGTGTGGAGAGATGGTAGATGCTAGGAGATTGTTCGATGAGATGGCCGTGAGGGATGTTCTTGCTTGGACCACACTGGTTTCGGGATATGCCAAATGGGGTGATATGAAATCAGCTAGCGAGTTGTTTGATCGGATGCCTGAGAAGAACCCGGTGTCATGGACATCTATGATTTCGGGGTATGCTAGGAATGGATTGGGGCATGAAGCGCTTGCATTGTTTGCAGAGATGATGCTGTTTCAAGTCAGGCCTGATCAGTTTACCTTTAGTAGTTGCCTTTGTGCTTGTGCTAGTATAGCTTCGCTTAAGCATGGTAAACAAATTCATGCCTCTTTAATACGAAATAATTTAAGACCCAACATGATTGTTGTGAGTTCTCTTATCGACATGTATTCCAAATGCGGCAGTTTGGGGGCCGGAAGGCAGGTTTTCAAGCTCATGGGTGATAAGCAAGACATCGTGTTATGGAACACAATGATGTCTGCCTTAGCACAGCATGGTCATGGTATAGAGGCATTGCAGATGTTTGAAGACATGGTCAGTTCAGGTGTAAAGCCGGTTACGACCACCTTTGTTGTCATTCTCAATGCTTGTAGTCATTCTGGTCTAGTGCAGGAAGGGCGTAGGATTTTCAAGTCCATGACTGATGATTATGGCATTGTTCCTGATGAGGAACATTATGCATGCTTAATTGATCTCTTGGGTCGAGCTGGATGTTTTGATGAGTTGATGAACCAGCTCGAAAATATGCCATGTAAAGCTGGCGGTCAAGTTTGGAATGCATTACTTGGTGTTTGTAGAATCCATGGAAATACAGAGCTGGGAAGAAAAGTGGCCGAACACCTTATTGAGTTGGAGCCTCAATCTTCTGCTCCCTATGTTTTGCTTTCGAGTATATATGCTGAAGAAGGTAGATGGGAGCTGGTAGAGAAGGTGAGACGGCTTATGGATGAGAGACATGTGAGGAAAGAGCGGGCCATTAGTTGGGTAGAAGTTGAAAGTAGAGTGCATGCTTTCACTGTATCAGACCGGCTGCATCCTCTGCAAGAGGAAATATACTCAGTTTTGAAACAGTTAGCTGACCAGATGGATGAAGATGCTTCAGTAACTAACGCCGAGAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

509

Amino Acids

57.45

Weight (kDa)

6.19

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR	PF01535	24 - 53	9.9e-07	PPR repeat
PPR_2	PF13041	52 - 101	7.3e-07	PPR repeat family
PPR	PF01535	55 - 83	3.3e-06	PPR repeat
PPR	PF01535	157 - 185	7.5e-07	PPR repeat
PPR_2	PF13041	185 - 232	8.9e-11	PPR repeat family
PPR	PF01535	187 - 213	2.5e-08	PPR repeat
PPR_2	PF13041	287 - 334	3.8e-11	PPR repeat family
PPR	PF01535	289 - 319	6.7e-08	PPR repeat
E_motif	PF20431	404 - 466	6.4e-16	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001363)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21090
fragaria_vesca	FvH4_1g26660
malus_domestica	MD05G1117900.v1.1 MD10G1120900.v1.1
prunus_persica	Prupe.8G162400_v2.0.a1
pyrus_communis	pycom05g11190 pycom10g10410 pycom10g10420
rosa_chinensis	RchiOBHm_Chr1g0323421 RchiOBHm_Chr1g0323511 RchiOBHm_Chr1g0323621 RchiOBHm_Chr1g0323681 RchiOBHm_Chr1g0323771
rosa_laevigata	RLG00000030297 RLG00000030299 RLG00000030300 RLG00000030302 RLG00000030317 RLG00000030324
rosa_multiflora	Rmu_sc0000252.1_g000001 Rmu_sc0000252.1_g000002 Rmu_sc0000252.1_g000009 Rmu_sc0000252.1_g000012 Rmu_sc0000252.1_g000023 Rmu_sc0000252.1_g000030 Rmu_sc0000804.1_g000006 Rmu_sc0002634.1_g000016 Rmu_sc0002634.1_g000019 Rmu_sc0008902.1_g000002 Rmu_sc0036879.1_g000001 Rmu_sc0036879.1_g000002
rosa_roxburghii	Rroxscaffold_4G00326420 Rroxscaffold_4G00326490 Rroxscaffold_4G00326520 Rroxscaffold_4G00326590
rosa_rugosa	Rorug01G0038100 Rorug01G0038200 Rorug01G0038300 Rorug01G0039100.1 Rorug01G0039200 Rorug01G0039500
rosa_samantha	Rh1AG055800 Rh1AG055900 Rh1AG056300 Rh1AG056400 Rh1AG056500 Rh1AG056700 Rh1BG047700 Rh1CG056700 Rh1CG058000 Rh1DG061800
rosa_wichuraiana	Rw1G004810 Rw1G004820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	269
Acc36I	ACCTGC	3 cut(s)	269, 331, 820
AccB7I	CCANNNNNTGG	1 cut(s)	1389
AccBSI	CCGCTC	1 cut(s)	1378
AciI	CCGC	5 cut(s)	15, 241, 807, 1170, 1378
AcoI	YGGCCR	2 cut(s)	450, 1239
AcsI	RAATTY	2 cut(s)	64, 726
AcuI	CTGAAG	3 cut(s)	137, 1329, 1491
AfaI	GTAC	2 cut(s)	5, 71
AfeI	AGCGCT	1 cut(s)	615
AfiI	CCNNNNNNNGG	3 cut(s)	201, 202, 1389
AflII	CTTAAG	1 cut(s)	710
AflIII	ACRYGT	2 cut(s)	792, 1363
AgsI	TTSAA	8 cut(s)	10, 262, 647, 838, 935, 1039, 1403, 1474
AjnI	CCWGG	1 cut(s)	167
AleI	CACNNNNGTG	1 cut(s)	179
Alw26I	GTCTC	2 cut(s)	1336, 1354
Aor51HI	AGCGCT	1 cut(s)	615
AoxI	GGCC	6 cut(s)	123, 450, 653, 819, 1239, 1380
ApeKI	GCWGC	1 cut(s)	1438
ApoI	RAATTY	2 cut(s)	64, 726
AspLEI	GCGC	1 cut(s)	616
AspS9I	GGNCC	4 cut(s)	124, 474, 819, 1380
AsuC2I	CCSGG	2 cut(s)	127, 557
AsuHPI	GGTGA	4 cut(s)	193, 518, 860, 1351
AsuNHI	GCTAGC	1 cut(s)	521
AvaII	GGWCC	1 cut(s)	474
BauI	CACGAG	1 cut(s)	73
BbsI	GAAGAC	1 cut(s)	942
BbvI	GCAGC	1 cut(s)	1425
BccI	CCATC	4 cut(s)	412, 442, 1314, 1486
BceAI	ACGGC	2 cut(s)	437, 1361
BcgI	CGANNNNNNTGC	2 cut(s)	355, 389
BciT130I	CCWGG	1 cut(s)	169
BclI	TGATCA	1 cut(s)	658
BcnI	CCSGG	2 cut(s)	127, 557
BcoDI	GTCTC	2 cut(s)	1336, 1354
BfaI	CTAG	6 cut(s)	429, 522, 591, 696, 1013, 1528
BfoI	RGCGCY	1 cut(s)	617
BfrI	CTTAAG	1 cut(s)	710
BfuAI	ACCTGC	3 cut(s)	269, 331, 820
BisI	GCNGC	3 cut(s)	16, 808, 1439
BlsI	GCNGC	3 cut(s)	17, 809, 1440
Bme1390I	CCNGG	3 cut(s)	127, 169, 557
Bme18I	GGWCC	1 cut(s)	474
BmgT120I	GGNCC	4 cut(s)	124, 474, 819, 1380
BmiI	GGNNCC	2 cut(s)	820, 1263
BmrFI	CCNGG	3 cut(s)	127, 169, 557
BmsI	GCATC	7 cut(s)	19, 385, 415, 531, 627, 1450, 1492
BmtI	GCTAGC	1 cut(s)	525
BpiI	GAAGAC	1 cut(s)	942
Bpu10I	CCTNAGC	1 cut(s)	892
BpuMI	CCSGG	2 cut(s)	127, 557
BsaJI	CCNNGG	2 cut(s)	177, 1210
BsaXI	ACNNNNNCTCC	2 cut(s)	405, 435
Bsc4I	CCNNNNNNNGG	3 cut(s)	201, 202, 1389
Bse118I	RCCGGY	3 cut(s)	250, 961, 1434
Bse1I	ACTGG	1 cut(s)	486
Bse3DI	GCAATG	1 cut(s)	920
BseBI	CCWGG	1 cut(s)	169
BseDI	CCNNGG	2 cut(s)	177, 1210
BseGI	GGATG	8 cut(s)	163, 400, 466, 546, 1124, 1360, 1441, 1501
BseLI	CCNNNNNNNGG	3 cut(s)	201, 202, 1389
BseMI	GCAATG	1 cut(s)	920
BseMII	CTCAG	3 cut(s)	141, 537, 1479
BseNI	ACTGG	1 cut(s)	486
BseXI	GCAGC	1 cut(s)	1425
BseYI	CCCAGC	2 cut(s)	353, 1225
BsgI	GTGCAG	1 cut(s)	1037
BshFI	GGCC	6 cut(s)	125, 452, 655, 821, 1241, 1382
BsiSI	CCGG	6 cut(s)	127, 251, 557, 822, 962, 1435
BslI	CCNNNNNNNGG	3 cut(s)	201, 202, 1389
BsmAI	GTCTC	2 cut(s)	1336, 1354
BsmBI	CGTCTC	1 cut(s)	1336
BsmI	GAATGC	1 cut(s)	1189
BsnI	GGCC	6 cut(s)	125, 452, 655, 821, 1241, 1382
Bsp143I	GATC	3 cut(s)	535, 658, 1099
Bsp19I	CCATGG	2 cut(s)	177, 1210
BspACI	CCGC	5 cut(s)	15, 241, 807, 1170, 1378
BspANI	GGCC	6 cut(s)	125, 452, 655, 821, 1241, 1382
BspCNI	CTCAG	3 cut(s)	142, 538, 1478
BspLI	GGNNCC	2 cut(s)	820, 1263
BspMI	ACCTGC	3 cut(s)	269, 331, 820
BspOI	GCTAGC	1 cut(s)	525
BspTI	CTTAAG	1 cut(s)	710
BsrBI	CCGCTC	1 cut(s)	1378
BsrDI	GCAATG	1 cut(s)	920
BsrFI	RCCGGY	3 cut(s)	250, 961, 1434
BsrI	ACTGG	1 cut(s)	486
BssAI	RCCGGY	3 cut(s)	250, 961, 1434
BssECI	CCNNGG	2 cut(s)	177, 1210
BssMI	GATC	3 cut(s)	535, 658, 1099
BssSI	CACGAG	1 cut(s)	73
BssT1I	CCWWGG	2 cut(s)	177, 1210
Bst2BI	CACGAG	1 cut(s)	73
Bst2UI	CCWGG	1 cut(s)	169
Bst4CI	ACNGT	3 cut(s)	292, 1426, 1479
BstAFI	CTTAAG	1 cut(s)	710
BstAPI	GCANNNNNTGC	2 cut(s)	626, 1447
BstC8I	GCNNGC	8 cut(s)	34, 330, 523, 618, 1090, 1168, 1380, 1416
BstDEI	CTNAG	4 cut(s)	150, 546, 892, 1465
BstDSI	CCRYGG	2 cut(s)	177, 1210
BstF5I	GGATG	8 cut(s)	163, 400, 466, 546, 1124, 1360, 1441, 1501
BstH2I	RGCGCY	1 cut(s)	617
BstHHI	GCGC	1 cut(s)	616
BstKTI	GATC	3 cut(s)	538, 661, 1102
BstMAI	GTCTC	2 cut(s)	1336, 1354
BstMBI	GATC	3 cut(s)	535, 658, 1099
BstMWI	GCNNNNNNNGC	7 cut(s)	247, 325, 613, 626, 701, 1024, 1447
BstNI	CCWGG	1 cut(s)	169
BstNSI	RCATGY	5 cut(s)	101, 796, 1092, 1367, 1418
BstSCI	CCNGG	3 cut(s)	125, 167, 555
BstV1I	GCAGC	1 cut(s)	1425
BstV2I	GAAGAC	1 cut(s)	942
BsuRI	GGCC	6 cut(s)	125, 452, 655, 821, 1241, 1382
BtgI	CCRYGG	2 cut(s)	177, 1210
BtgZI	GCGATG	1 cut(s)	33
BtsCI	GGATG	8 cut(s)	163, 400, 466, 546, 1124, 1360, 1441, 1501
BtsIMutI	CAGTG	2 cut(s)	479, 1422
BveI	ACCTGC	3 cut(s)	269, 331, 820
Cac8I	GCNNGC	8 cut(s)	34, 330, 523, 618, 1090, 1168, 1380, 1416
CfoI	GCGC	1 cut(s)	616
Cfr10I	RCCGGY	3 cut(s)	250, 961, 1434
Cfr13I	GGNCC	4 cut(s)	124, 474, 819, 1380
Csp6I	GTAC	2 cut(s)	4, 70
CspCI	CAANNNNNGTGG	2 cut(s)	961, 996
CviQI	GTAC	2 cut(s)	4, 70
DdeI	CTNAG	4 cut(s)	150, 546, 892, 1465
DpnI	GATC	3 cut(s)	537, 660, 1101
DpnII	GATC	3 cut(s)	535, 658, 1099
EaeI	YGGCCR	2 cut(s)	450, 1239
Eco130I	CCWWGG	2 cut(s)	177, 1210
Eco147I	AGGCCT	1 cut(s)	655
Eco47I	GGWCC	1 cut(s)	474
Eco47III	AGCGCT	1 cut(s)	615
Eco57I	CTGAAG	3 cut(s)	137, 1329, 1491
EcoRII	CCWGG	1 cut(s)	167
EcoT14I	CCWWGG	2 cut(s)	177, 1210
EcoT22I	ATGCAT	2 cut(s)	1090, 1189
ErhI	CCWWGG	2 cut(s)	177, 1210
Esp3I	CGTCTC	1 cut(s)	1336
FauI	CCCGC	1 cut(s)	1371
FbaI	TGATCA	1 cut(s)	658
Fnu4HI	GCNGC	3 cut(s)	16, 808, 1439
FokI	GGATG	8 cut(s)	170, 407, 473, 553, 1131, 1367, 1428, 1508
Fsp4HI	GCNGC	3 cut(s)	16, 808, 1439
FspBI	CTAG	6 cut(s)	429, 522, 591, 696, 1013, 1528
GlaI	GCGC	1 cut(s)	615
GluI	GCNGC	3 cut(s)	16, 808, 1439
GsaI	CCCAGC	2 cut(s)	357, 1229
HaeII	RGCGCY	1 cut(s)	617
HaeIII	GGCC	6 cut(s)	125, 452, 655, 821, 1241, 1382
HapII	CCGG	6 cut(s)	127, 251, 557, 822, 962, 1435
HhaI	GCGC	1 cut(s)	616
Hin6I	GCGC	1 cut(s)	614
HinP1I	GCGC	1 cut(s)	614
HindIII	AAGCTT	1 cut(s)	317
HinfI	GANTC	2 cut(s)	112, 1206
HpaII	CCGG	6 cut(s)	127, 251, 557, 822, 962, 1435
HphI	GGTGA	4 cut(s)	193, 518, 860, 1351
Hpy166II	GTNNAC	2 cut(s)	666, 722
Hpy188I	TCNGA	3 cut(s)	117, 540, 1432
Hpy188III	TCNNGA	2 cut(s)	489, 1070
Hpy8I	GTNNAC	2 cut(s)	666, 722
HpyAV	CCTTC	5 cut(s)	112, 819, 1016, 1307, 1330
HpyCH4III	ACNGT	3 cut(s)	292, 1426, 1479
HpyF10VI	GCNNNNNNNGC	7 cut(s)	247, 325, 613, 626, 701, 1024, 1447
HpyF3I	CTNAG	4 cut(s)	150, 546, 892, 1465
HspAI	GCGC	1 cut(s)	614
Ksp22I	TGATCA	1 cut(s)	658
Kzo9I	GATC	3 cut(s)	535, 658, 1099
LmnI	GCTCC	4 cut(s)	129, 1261, 1282, 1324
Lsp1109I	GCAGC	1 cut(s)	1425
LweI	GCATC	7 cut(s)	19, 385, 415, 531, 627, 1450, 1492
MaeI	CTAG	6 cut(s)	429, 522, 591, 696, 1013, 1528
MaeIII	GTNAC	2 cut(s)	964, 1510
MalI	GATC	3 cut(s)	537, 660, 1101
MbiI	CCGCTC	1 cut(s)	1378
MboI	GATC	3 cut(s)	535, 658, 1099
MboII	GAAGA	8 cut(s)	115, 313, 562, 947, 1242, 1263, 1322, 1511
MluCI	AATT	4 cut(s)	64, 726, 751, 1095
MmeI	TCCRAC	1 cut(s)	1239
Mph1103I	ATGCAT	2 cut(s)	1090, 1189
MseI	TTAA	4 cut(s)	711, 740, 755, 1094
MslI	CAYNNNNRTG	3 cut(s)	179, 881, 906
MspCI	CTTAAG	1 cut(s)	710
MspI	CCGG	6 cut(s)	127, 251, 557, 822, 962, 1435
MspR9I	CCNGG	3 cut(s)	127, 169, 557
Mva1269I	GAATGC	1 cut(s)	1189
MvaI	CCWGG	1 cut(s)	169
MwoI	GCNNNNNNNGC	7 cut(s)	247, 325, 613, 626, 701, 1024, 1447
NciI	CCSGG	2 cut(s)	127, 557
NcoI	CCATGG	2 cut(s)	177, 1210
NdeII	GATC	3 cut(s)	535, 658, 1099
NheI	GCTAGC	1 cut(s)	521
NlaIV	GGNNCC	2 cut(s)	820, 1263
NsiI	ATGCAT	2 cut(s)	1090, 1189
NspI	RCATGY	5 cut(s)	101, 796, 1092, 1367, 1418
OliI	CACNNNNGTG	1 cut(s)	179
PaeI	GCATGC	2 cut(s)	1092, 1418
PaqCI	CACCTGC	1 cut(s)	269
PceI	AGGCCT	1 cut(s)	655
PciI	ACATGT	2 cut(s)	792, 1363
PctI	GAATGC	1 cut(s)	1189
PfeI	GAWTC	2 cut(s)	112, 1206
PflFI	GACNNNGTC	1 cut(s)	941
PflMI	CCANNNNNTGG	1 cut(s)	1389
PfoI	TCCNGGA	1 cut(s)	167
PkrI	GCNGC	3 cut(s)	17, 809, 1440
PscI	ACATGT	2 cut(s)	792, 1363
Psp6I	CCWGG	1 cut(s)	167
PspFI	CCCAGC	2 cut(s)	353, 1225
PspGI	CCWGG	1 cut(s)	167
PspN4I	GGNNCC	2 cut(s)	820, 1263
PspPI	GGNCC	4 cut(s)	124, 474, 819, 1380
PsyI	GACNNNGTC	1 cut(s)	941
RsaI	GTAC	2 cut(s)	5, 71
RsaNI	GTAC	2 cut(s)	4, 70
RseI	CAYNNNNRTG	3 cut(s)	179, 881, 906
SaqAI	TTAA	4 cut(s)	711, 740, 755, 1094
SatI	GCNGC	3 cut(s)	16, 808, 1439
Sau3AI	GATC	3 cut(s)	535, 658, 1099
Sau96I	GGNCC	4 cut(s)	124, 474, 819, 1380
ScrFI	CCNGG	3 cut(s)	127, 169, 557
SfaNI	GCATC	7 cut(s)	19, 385, 415, 531, 627, 1450, 1492
SinI	GGWCC	1 cut(s)	474
SmiMI	CAYNNNNRTG	3 cut(s)	179, 881, 906
SmlI	CTYRAG	1 cut(s)	710
SmoI	CTYRAG	1 cut(s)	710
SphI	GCATGC	2 cut(s)	1092, 1418
Sse9I	AATT	4 cut(s)	64, 726, 751, 1095
SseBI	AGGCCT	1 cut(s)	655
SsiI	CCGC	5 cut(s)	15, 241, 807, 1170, 1378
SspMI	CTAG	6 cut(s)	429, 522, 591, 696, 1013, 1528
StuI	AGGCCT	1 cut(s)	655
StyD4I	CCNGG	3 cut(s)	125, 167, 555
StyI	CCWWGG	2 cut(s)	177, 1210
TaaI	ACNGT	3 cut(s)	292, 1426, 1479
TaqI	TCGA	7 cut(s)	365, 380, 441, 789, 1111, 1146, 1295
TasI	AATT	4 cut(s)	64, 726, 751, 1095
TatI	WGTACW	2 cut(s)	3, 69
TauI	GCSGC	2 cut(s)	18, 810
TfiI	GAWTC	2 cut(s)	112, 1206
Tru1I	TTAA	4 cut(s)	711, 740, 755, 1094
Tru9I	TTAA	4 cut(s)	711, 740, 755, 1094
TscAI	CASTG	2 cut(s)	486, 1429
TseI	GCWGC	1 cut(s)	1438
TspDTI	ATGAA	5 cut(s)	527, 624, 719, 1151, 1512
TspRI	CASTG	2 cut(s)	486, 1429
Tth111I	GACNNNGTC	1 cut(s)	941
Van91I	CCANNNNNTGG	1 cut(s)	1389
Vha464I	CTTAAG	1 cut(s)	710
VpaK11BI	GGWCC	1 cut(s)	474
XapI	RAATTY	2 cut(s)	64, 726
XceI	RCATGY	5 cut(s)	101, 796, 1092, 1367, 1418
XspI	CTAG	6 cut(s)	429, 522, 591, 696, 1013, 1528
Zsp2I	ATGCAT	2 cut(s)	1090, 1189

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.