RchiOBHm_Chr1g0356121

Cation H( ) antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001192
Physical Location & Seq
Reverse (-)
48638738 .. 48641523
2786 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1620 bp
ATGGCAGGGATGACTGGAAACATAACATCATTGCAATCTCAAGTATGCATTGAATTACCTCCCAACGTACATTCGCAGGGCATCATAGGTCTGGAAAAAGACGGTCCAGGAATGAACTTTAGTTTTCCCATATTGGAGATGCAGATGATCCTGATTTTCTCCATCACCCAAGCCTTCCATTTGATTTTGAAGAATTTTGAAATTCCCAAGTTAACCAGCGAGATTCTTACGGGTATAGTCCTAGGCCCTTCATTCATAGGAGCCTATAATGTATTCGAGAAGAACATCTTTCCTCGTGACAATCAAGCTATAATTGTAGTGCTCGGTGAAATTGGTTATGAACTCTTTTTGTTTCTAGTTGGAGTAAGAATGGACCTGGAATTGATAAGAAGAACCGGGCGCAAAGTCATGATCACCGGTATTTTATGCGTGTTAGTCCCTTTTGTAACCAGCATGGTCGTCCAAAACCATCTCATGATCCCTGTGTTTAAGCTGACCAAAGATCAAATCTCTAAGCTTCCTTATCTAACGACATCATATTGCATGACTTCGTCTCCCGTCATTGTTCTCCTTCTTGAAGACCTGAATCTCCTAAATTCTGAAATCGGCCGACTAGCTGTGTCCGCATCATCAATCAGTGAATTATTCGGTACTGTCTGTATCTGGTTTTCCTCCGTATTGTACAGAATGGGAAAAGTGAAACCTGTGGCTGAAACTTTATTAGTTTTCGGAGCAGTTATTGGCAGCATGCTAGCATTTCTGTTCATATTCCGACCAATACTGTTTTGGGTGATTAGGCAGACCCCGCATAACAAGCCTGTTAAGAAAAGCTATGTCAATGCCATAATCGTGCTGATGCTTTTTGTAATAATACTATCACATTATTACGGCCATTCTTTCCATTTCCTGGTTTTCGTATTGGGTCTGTCAGTCCCAGCTGGACAACCTTTGGGATCAACTCTTGAAGAATTGCTCCATGTCTTCATTTCTCAAGTGCTTCTACCCATTTATGTGACTGCATATGCAATGAAGGCAAATTTTCGGTTGGTCGATTTCAATGACCCGGCCACAGTAATCACTGCTATTCTCATCGTTTTCATTTTTCTGCTCAAAATCATAGGCAGCATGGTTGCTCCATTGTATTGTAAAATGCCCTTGAACGATGCATTTGTGCTTGCTCTCATTCTAAGCTCCAGAGGCATTGTCCAACTTTCCGTCTATACTCGATTCAAAGACGATCAGACTGCAAGTGAATCGGTACATGCTTTGAGTCTTGCTAGCATTGTAGTGACTGCAACTCTTGTGCCCATTGTCGTGAACCGCCTCTATGATCCTGCAAAGAAATACAGAGTCTACGAAAGAAGGGATATTATGCATTTGAAACCCAATGCTGAGCTCAAAATTCTTGCCTGCATTCACAGGTCAGGTAACATTCCTCCAGTCATCAATCTACTTGATGCAGCATGTCCATCTAAAGAAAACCCCATTGATATATATGTTCTTCACCTCATCGAACTAATTGGAAGAAGCTCCCCAGTTTTTATCTCCCACCAAATGCAGAAAAGAGCATTTCCAATGGTTACTCGGACAATGTCATTCTTTACTTCAATAATTTCGTGA

Protein Analysis

539

Amino Acids

60.14

Weight (kDa)

9.08

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 58 - 439 3.5e-34 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 907
AccI GTMKAC 1 cut(s) 1353
AciI CCGC 3 cut(s) 624, 806, 1321
AclWI GGATC 4 cut(s) 142, 472, 961, 1325
AcoI YGGCCR 3 cut(s) 607, 889, 1065
AcsI RAATTY 5 cut(s) 193, 201, 595, 1036, 1401
AfaI GTAC 4 cut(s) 69, 652, 683, 1260
AfiI CCNNNNNNNGG 1 cut(s) 907
AgeI ACCGGT 1 cut(s) 416
AjnI CCWGG 3 cut(s) 106, 375, 906
AluBI AGCT 9 cut(s) 308, 493, 517, 617, 831, 938, 1191, 1396, 1530
AluI AGCT 9 cut(s) 308, 493, 517, 617, 831, 938, 1191, 1396, 1530
Alw21I GWGCWC 2 cut(s) 324, 1398
Alw26I GTCTC 1 cut(s) 558
AlwI GGATC 4 cut(s) 142, 472, 961, 1325
AoxI GGCC 4 cut(s) 244, 607, 889, 1065
ApeKI GCWGC 3 cut(s) 744, 1122, 1460
ApoI RAATTY 5 cut(s) 193, 201, 595, 1036, 1401
AsiGI ACCGGT 1 cut(s) 416
AspA2I CCTAGG 1 cut(s) 241
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 3 cut(s) 104, 245, 373
AsuC2I CCSGG 2 cut(s) 397, 1064
AsuHPI GGTGA 5 cut(s) 157, 338, 406, 802, 1496
AsuNHI GCTAGC 2 cut(s) 751, 1277
AvaII GGWCC 2 cut(s) 104, 373
AvrII CCTAGG 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 1308
BanII GRGCYC 1 cut(s) 1398
BauI CACGAG 1 cut(s) 294
BbsI GAAGAC 2 cut(s) 585, 973
Bbv12I GWGCWC 2 cut(s) 324, 1398
BbvI GCAGC 3 cut(s) 756, 1134, 1472
BccI CCATC 3 cut(s) 170, 477, 1477
BceAI ACGGC 1 cut(s) 904
BcgI CGANNNNNNTGC 4 cut(s) 1226, 1236, 1260, 1270
BciT130I CCWGG 3 cut(s) 108, 377, 908
BclI TGATCA 1 cut(s) 411
BcnI CCSGG 2 cut(s) 397, 1064
BcoDI GTCTC 1 cut(s) 558
BfaI CTAG 5 cut(s) 242, 356, 614, 752, 1278
BisI GCNGC 3 cut(s) 745, 1123, 1461
BlnI CCTAGG 1 cut(s) 241
BlpI GCTNAGC 1 cut(s) 1392
BlsI GCNGC 3 cut(s) 746, 1124, 1462
Bme1390I CCNGG 5 cut(s) 108, 377, 397, 908, 1064
Bme18I GGWCC 2 cut(s) 104, 373
BmgT120I GGNCC 3 cut(s) 104, 245, 373
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 5 cut(s) 108, 377, 397, 908, 1064
BmrI ACTGGG 1 cut(s) 1529
BmsI GCATC 6 cut(s) 90, 129, 635, 846, 1153, 1447
BmtI GCTAGC 2 cut(s) 755, 1281
BmuI ACTGGG 1 cut(s) 1529
BpiI GAAGAC 2 cut(s) 585, 973
BpmI CTGGAG 2 cut(s) 1177, 1422
Bpu1102I GCTNAGC 1 cut(s) 1392
BpuEI CTTGAG 2 cut(s) 24, 975
BpuMI CCSGG 2 cut(s) 397, 1064
BsaJI CCNNGG 1 cut(s) 241
BsaWI WCCGGW 1 cut(s) 416
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 1 cut(s) 907
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 3 cut(s) 19, 1439, 1535
Bse3DI GCAATG 2 cut(s) 29, 1032
BseBI CCWGG 3 cut(s) 108, 377, 908
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 907
BseMI GCAATG 2 cut(s) 29, 1032
BseMII CTCAG 1 cut(s) 1383
BseNI ACTGG 3 cut(s) 19, 1439, 1535
BseSI GKGCMC 1 cut(s) 1308
BseX3I CGGCCG 1 cut(s) 607
BseXI GCAGC 3 cut(s) 756, 1134, 1472
BseYI CCCAGC 1 cut(s) 934
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 4 cut(s) 246, 609, 891, 1067
BshTI ACCGGT 1 cut(s) 416
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 2 cut(s) 324, 1398
BsiSI CCGG 3 cut(s) 396, 417, 1064
BslFI GGGAC 2 cut(s) 422, 917
BslI CCNNNNNNNGG 1 cut(s) 907
BsmAI GTCTC 1 cut(s) 558
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 2 cut(s) 422, 917
BsmI GAATGC 1 cut(s) 1413
BsnI GGCC 4 cut(s) 246, 609, 891, 1067
Bsp1286I GDGCHC 3 cut(s) 324, 1308, 1398
Bsp1407I TGTACA 1 cut(s) 681
Bsp143I GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
Bsp1720I GCTNAGC 1 cut(s) 1392
BspACI CCGC 3 cut(s) 624, 806, 1321
BspANI GGCC 4 cut(s) 246, 609, 891, 1067
BspCNI CTCAG 1 cut(s) 1384
BspHI TCATGA 2 cut(s) 408, 474
BspLI GGNNCC 1 cut(s) 262
BspOI GCTAGC 2 cut(s) 755, 1281
BspPI GGATC 4 cut(s) 142, 472, 961, 1325
BsrDI GCAATG 2 cut(s) 29, 1032
BsrFI RCCGGY 1 cut(s) 416
BsrGI TGTACA 1 cut(s) 681
BsrI ACTGG 3 cut(s) 19, 1439, 1535
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
BssSI CACGAG 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 241
Bst2BI CACGAG 1 cut(s) 294
Bst2UI CCWGG 3 cut(s) 108, 377, 908
Bst4CI ACNGT 4 cut(s) 104, 655, 783, 1072
BstAUI TGTACA 1 cut(s) 681
BstC8I GCNNGC 5 cut(s) 749, 753, 1176, 1279, 1411
BstDEI CTNAG 3 cut(s) 513, 1187, 1392
BstF5I GGATG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 7 cut(s) 150, 414, 480, 505, 956, 1240, 1333
BstMAI GTCTC 1 cut(s) 558
BstMBI GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
BstMCI CGRYCG 1 cut(s) 610
BstMWI GCNNNNNNNGC 5 cut(s) 623, 805, 814, 1031, 1197
BstNI CCWGG 3 cut(s) 108, 377, 908
BstNSI RCATGY 3 cut(s) 751, 1265, 1467
BstSCI CCNGG 5 cut(s) 106, 375, 395, 906, 1062
BstSLI GKGCMC 1 cut(s) 1308
BstV1I GCAGC 3 cut(s) 756, 1134, 1472
BstV2I GAAGAC 2 cut(s) 585, 973
BstZI CGGCCG 1 cut(s) 607
BsuRI GGCC 4 cut(s) 246, 609, 891, 1067
BtsCI GGATG 1 cut(s) 15
BtsI GCAGTG 1 cut(s) 1077
BtsIMutI CAGTG 2 cut(s) 643, 1077
Cac8I GCNNGC 5 cut(s) 749, 753, 1176, 1279, 1411
CciI TCATGA 2 cut(s) 408, 474
CfoI GCGC 1 cut(s) 402
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 3 cut(s) 104, 245, 373
Csp6I GTAC 4 cut(s) 68, 651, 682, 1259
CspAI ACCGGT 1 cut(s) 416
CviAII CATG 9 cut(s) 409, 454, 475, 544, 748, 977, 1126, 1262, 1464
CviQI GTAC 4 cut(s) 68, 651, 682, 1259
DdeI CTNAG 3 cut(s) 513, 1187, 1392
DpnI GATC 7 cut(s) 149, 413, 479, 504, 955, 1239, 1332
DpnII GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
EaeI YGGCCR 3 cut(s) 607, 889, 1065
EagI CGGCCG 1 cut(s) 607
Ecl136II GAGCTC 1 cut(s) 1396
EclXI CGGCCG 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 1398
Eco47I GGWCC 2 cut(s) 104, 373
Eco52I CGGCCG 1 cut(s) 607
Eco53kI GAGCTC 1 cut(s) 1396
EcoICRI GAGCTC 1 cut(s) 1396
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 3 cut(s) 106, 375, 906
EcoT14I CCWWGG 1 cut(s) 241
EcoT22I ATGCAT 3 cut(s) 50, 1168, 1377
EcoT38I GRGCYC 1 cut(s) 1398
ErhI CCWWGG 1 cut(s) 241
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 9 cut(s) 412, 457, 478, 547, 751, 980, 1129, 1265, 1467
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 2 cut(s) 422, 917
FatI CATG 9 cut(s) 408, 453, 474, 543, 747, 976, 1125, 1261, 1463
FauI CCCGC 1 cut(s) 813
FauNDI CATATG 1 cut(s) 1021
FbaI TGATCA 1 cut(s) 411
FblI GTMKAC 1 cut(s) 1353
Fnu4HI GCNGC 3 cut(s) 745, 1123, 1461
FokI GGATG 1 cut(s) 22
FriOI GRGCYC 1 cut(s) 1398
Fsp4HI GCNGC 3 cut(s) 745, 1123, 1461
FspBI CTAG 5 cut(s) 242, 356, 614, 752, 1278
GlaI GCGC 1 cut(s) 401
GluI GCNGC 3 cut(s) 745, 1123, 1461
GsaI CCCAGC 1 cut(s) 938
GsuI CTGGAG 2 cut(s) 1177, 1422
HaeIII GGCC 4 cut(s) 246, 609, 891, 1067
HapII CCGG 3 cut(s) 396, 417, 1064
HhaI GCGC 1 cut(s) 402
Hin1II CATG 9 cut(s) 412, 457, 478, 547, 751, 980, 1129, 1265, 1467
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HincII GTYRAC 1 cut(s) 213
HindII GTYRAC 1 cut(s) 213
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 6 cut(s) 223, 586, 1227, 1253, 1270, 1350
HpaI GTTAAC 1 cut(s) 213
HpaII CCGG 3 cut(s) 396, 417, 1064
HphI GGTGA 5 cut(s) 157, 338, 406, 802, 1496
Hpy166II GTNNAC 3 cut(s) 213, 1318, 1354
Hpy188I TCNGA 5 cut(s) 601, 731, 773, 1242, 1587
Hpy8I GTNNAC 3 cut(s) 213, 1318, 1354
HpyAV CCTTC 5 cut(s) 184, 258, 581, 1024, 1356
HpyCH4III ACNGT 4 cut(s) 104, 655, 783, 1072
HpyCH4IV ACGT 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 5 cut(s) 623, 805, 814, 1031, 1197
HpyF3I CTNAG 3 cut(s) 513, 1187, 1392
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 9 cut(s) 412, 457, 478, 547, 751, 980, 1129, 1265, 1467
HspAI GCGC 1 cut(s) 400
Ksp22I TGATCA 1 cut(s) 411
KspAI GTTAAC 1 cut(s) 213
Kzo9I GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
LmnI GCTCC 6 cut(s) 260, 731, 978, 1138, 1196, 1535
Lsp1109I GCAGC 3 cut(s) 756, 1134, 1472
LweI GCATC 6 cut(s) 90, 129, 635, 846, 1153, 1447
MaeI CTAG 5 cut(s) 242, 356, 614, 752, 1278
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 6 cut(s) 296, 445, 1012, 1288, 1427, 1579
MalI GATC 7 cut(s) 149, 413, 479, 504, 955, 1239, 1332
MboI GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
MboII GAAGA 8 cut(s) 202, 292, 402, 590, 973, 977, 1493, 1536
MhlI GDGCHC 3 cut(s) 324, 1308, 1398
MlyI GAGTC 2 cut(s) 1279, 1359
MmeI TCCRAC 3 cut(s) 340, 796, 1231
MnlI CCTC 7 cut(s) 69, 303, 682, 1190, 1334, 1446, 1517
Mph1103I ATGCAT 3 cut(s) 50, 1168, 1377
MseI TTAA 3 cut(s) 212, 489, 822
MslI CAYNNNNRTG 4 cut(s) 848, 1010, 1286, 1313
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 3 cut(s) 396, 417, 1064
MspR9I CCNGG 5 cut(s) 108, 377, 397, 908, 1064
Mva1269I GAATGC 1 cut(s) 1413
MvaI CCWGG 3 cut(s) 108, 377, 908
MwoI GCNNNNNNNGC 5 cut(s) 623, 805, 814, 1031, 1197
NciI CCSGG 2 cut(s) 397, 1064
NdeI CATATG 1 cut(s) 1021
NdeII GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
NheI GCTAGC 2 cut(s) 751, 1277
NlaIII CATG 9 cut(s) 412, 457, 478, 547, 751, 980, 1129, 1265, 1467
NlaIV GGNNCC 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 296, 1012, 1288
NsiI ATGCAT 3 cut(s) 50, 1168, 1377
NspI RCATGY 3 cut(s) 751, 1265, 1467
PaeI GCATGC 1 cut(s) 751
PagI TCATGA 2 cut(s) 408, 474
PctI GAATGC 1 cut(s) 1413
PfeI GAWTC 4 cut(s) 223, 586, 1227, 1253
PflFI GACNNNGTC 2 cut(s) 550, 1591
PflMI CCANNNNNTGG 1 cut(s) 907
PfoI TCCNGGA 1 cut(s) 106
PinAI ACCGGT 1 cut(s) 416
PkrI GCNGC 3 cut(s) 746, 1124, 1462
PleI GAGTC 2 cut(s) 1278, 1358
PpsI GAGTC 2 cut(s) 1278, 1358
Psp124BI GAGCTC 1 cut(s) 1398
Psp6I CCWGG 3 cut(s) 106, 375, 906
PspFI CCCAGC 1 cut(s) 934
PspGI CCWGG 3 cut(s) 106, 375, 906
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 3 cut(s) 104, 245, 373
PsyI GACNNNGTC 2 cut(s) 550, 1591
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 4 cut(s) 69, 652, 683, 1260
RsaNI GTAC 4 cut(s) 68, 651, 682, 1259
RseI CAYNNNNRTG 4 cut(s) 848, 1010, 1286, 1313
SacI GAGCTC 1 cut(s) 1398
SaqAI TTAA 3 cut(s) 212, 489, 822
SatI GCNGC 3 cut(s) 745, 1123, 1461
Sau3AI GATC 7 cut(s) 147, 411, 477, 502, 953, 1237, 1330
Sau96I GGNCC 3 cut(s) 104, 245, 373
SchI GAGTC 2 cut(s) 1279, 1359
ScrFI CCNGG 5 cut(s) 108, 377, 397, 908, 1064
SduI GDGCHC 3 cut(s) 324, 1308, 1398
SfaNI GCATC 6 cut(s) 90, 129, 635, 846, 1153, 1447
SinI GGWCC 2 cut(s) 104, 373
SmiMI CAYNNNNRTG 4 cut(s) 848, 1010, 1286, 1313
SmlI CTYRAG 2 cut(s) 39, 990
SmoI CTYRAG 2 cut(s) 39, 990
SphI GCATGC 1 cut(s) 751
SsiI CCGC 3 cut(s) 624, 806, 1321
SspMI CTAG 5 cut(s) 242, 356, 614, 752, 1278
SstI GAGCTC 1 cut(s) 1398
StyD4I CCNGG 5 cut(s) 106, 375, 395, 906, 1062
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 4 cut(s) 104, 655, 783, 1072
TaiI ACGT 1 cut(s) 69
TaqI TCGA 4 cut(s) 276, 1050, 1225, 1512
TatI WGTACW 1 cut(s) 681
TfiI GAWTC 4 cut(s) 223, 586, 1227, 1253
Tru1I TTAA 3 cut(s) 212, 489, 822
Tru9I TTAA 3 cut(s) 212, 489, 822
TscAI CASTG 2 cut(s) 643, 1084
TseFI GTSAC 3 cut(s) 296, 1012, 1288
TseI GCWGC 3 cut(s) 744, 1122, 1460
Tsp45I GTSAC 3 cut(s) 296, 1012, 1288
TspDTI ATGAA 8 cut(s) 128, 240, 244, 354, 754, 973, 1043, 1087
TspGWI ACGGA 2 cut(s) 664, 1204
TspRI CASTG 2 cut(s) 643, 1084
Tth111I GACNNNGTC 2 cut(s) 550, 1591
Van91I CCANNNNNTGG 1 cut(s) 907
VpaK11BI GGWCC 2 cut(s) 104, 373
XapI RAATTY 5 cut(s) 193, 201, 595, 1036, 1401
XceI RCATGY 3 cut(s) 751, 1265, 1467
XcmI CCANNNNNNNNNTGG 1 cut(s) 783
XmaJI CCTAGG 1 cut(s) 241
XmiI GTMKAC 1 cut(s) 1353
XspI CTAG 5 cut(s) 242, 356, 614, 752, 1278
Zsp2I ATGCAT 3 cut(s) 50, 1168, 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.