RchiOBHm_Chr4g0423881

Major allergen Pru

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000091
Physical Location & Seq
Reverse (-)
49475479 .. 49477053
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39321

Sequence Viewer

Length: 684 bp
ATGTTGGTAATGGCTGGTGATTATAACCGAACTTTGTTCAAAGTTGTCATAGGACAGTATTGTCAGTTCACTCGGGAAATCTTCACTCTCAAAATCTATAAAGACCACCTTCCCGTTACACATTTCCAACACAACACAGCTCCCCTAAAACCATCATCCTTGAAGTTTCTTCCTTTGCTTATTTCTCAAGTCGTGATCATCATGGGTGTTTACACTTATGAAAGCGAGTTCACCTCCGACATCCCACCACCTAGGTTGTTCAAGGCCTTCGTTCTTGATGCTGACCACCTCATCCCCAAGATTGCACCTCAGGCAGTCAAGTCCGCTGAGATCCTCGAAGGAGATGGCGGCCCCGGAACCCTCAAGAAGATCACTTTTGGCGAAGGCAGCCACTACGGGTACGTGAAGCACAAGATCCACTCCATTGACAAAGAGAACCACGTCTACAGCTACAGTTTGGTCGAGGGAGATGCCTTGTCAGACACCATCGAGAAGATCGACTATGAGACCAAGTTGGTGGCAGCTCCTCACGGAGGAACCATCATCAAGAGCACCAGCAAGTACCACACCAAGGGTGATGTCGAAATCAAGGAAGAGCACGTCAAGGCCGGCAAAGAGAAGGCCTCGCACCTCTTCAAGCTCATTGAGGGATACCTCAAGGACCACCCCAGCGAATACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

227

Amino Acids

25.53

Weight (kDa)

6.9

Isoelectric Point (pI)

20.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 68 - 220 1.3e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000091)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18830 FvH4_4g18850 FvH4_4g18851 FvH4_4g18990 FvH4_4g19000 FvH4_4g19010 FvH4_4g19020 FvH4_4g19040 FvH4_4g19050 FvH4_4g19060 FvH4_4g19080 FvH4_4g19120 FvH4_4g19170 FvH4_4g19700 FvH4_4g19710 FvH4_5g00780
malus_domestica MD13G1160600.v1.1 MD13G1160700.v1.1 MD13G1160900.v1.1 MD13G1161000.v1.1 MD13G1161100.v1.1 MD13G1161200.v1.1 MD13G1161300.v1.1 MD13G1161400.v1.1 MD16G1159900.v1.1 MD16G1160000.v1.1 MD16G1160100.v1.1 MD16G1160300.v1.1 MD16G1160400.v1.1 MD16G1160500.v1.1 MD16G1160700.v1.1 MD16G1161100.v1.1 MD16G1161200.v1.1 MD16G1161400.v1.1 MD16G1161500.v1.1
prunus_persica Prupe.1G126900_v2.0.a1 Prupe.1G126900_v2.0.a1 Prupe.1G127100_v2.0.a1 Prupe.1G127200_v2.0.a1 Prupe.1G127300_v2.0.a1 Prupe.1G127400_v2.0.a1 Prupe.1G127500_v2.0.a1 Prupe.1G127600_v2.0.a1 Prupe.1G127600_v2.0.a1 Prupe.1G127700_v2.0.a1 Prupe.1G127800_v2.0.a1 Prupe.1G127900_v2.0.a1 Prupe.1G128100_v2.0.a1 Prupe.1G128200_v2.0.a1 Prupe.1G128300_v2.0.a1 Prupe.1G128500_v2.0.a1 Prupe.1G128600_v2.0.a1 Prupe.1G128700_v2.0.a1 Prupe.6G094400_v2.0.a1
pyrus_communis pycom06g07350 pycom08g13110 pycom08g13120 pycom13g13660 pycom13g13680 pycom13g13700 pycom13g13710 pycom13g13730 pycom13g13740 pycom13g13750 pycom13g13760 pycom16g13470 pycom16g13480 pycom16g13540 pycom16g13580 pycom16g13600 pycom16g13650 pycom16g13690
rosa_chinensis RchiOBHm_Chr4g0410071 RchiOBHm_Chr4g0410081 RchiOBHm_Chr4g0410091 RchiOBHm_Chr4g0410251 RchiOBHm_Chr4g0410281 RchiOBHm_Chr4g0410291 RchiOBHm_Chr4g0423641 RchiOBHm_Chr4g0423661 RchiOBHm_Chr4g0423691 RchiOBHm_Chr4g0423701 RchiOBHm_Chr4g0423711 RchiOBHm_Chr4g0423721 RchiOBHm_Chr4g0423881 RchiOBHm_Chr4g0423891 RchiOBHm_Chr4g0423901 RchiOBHm_Chr4g0423911 RchiOBHm_Chr4g0423921 RchiOBHm_Chr4g0423931 RchiOBHm_Chr4g0423941 RchiOBHm_Chr4g0423961 RchiOBHm_Chr4g0423971 RchiOBHm_Chr4g0423991 RchiOBHm_Chr4g0424011 RchiOBHm_Chr4g0424021 RchiOBHm_Chr4g0424031 RchiOBHm_Chr4g0424041 RchiOBHm_Chr4g0424121 RchiOBHm_Chr4g0424131 RchiOBHm_Chr4g0425171 RchiOBHm_Chr4g0425181 RchiOBHm_Chr7g0201401
rosa_laevigata RLG00000003678 RLG00000007393 RLG00000007394 RLG00000007502 RLG00000007503 RLG00000007508 RLG00000007509 RLG00000007510 RLG00000007511 RLG00000007512 RLG00000007514 RLG00000007515 RLG00000007516 RLG00000007517 RLG00000007518 RLG00000007520 RLG00000007521 RLG00000007523 RLG00000007535 RLG00000007536 RLG00000007537 RLG00000007538 RLG00000007539 RLG00000008440 RLG00000008441 RLG00000008491 RLG00000008504
rosa_multiflora Rmu_co8215346.1_g000001 Rmu_sc0000365.1_g000047 Rmu_sc0000365.1_g000054 Rmu_sc0000365.1_g000055 Rmu_sc0000464.1_g000004 Rmu_sc0000464.1_g000005 Rmu_sc0000464.1_g000006 Rmu_sc0000464.1_g000007 Rmu_sc0000464.1_g000009 Rmu_sc0000464.1_g000012 Rmu_sc0000464.1_g000013 Rmu_sc0000464.1_g000015 Rmu_sc0000554.1_g000007 Rmu_sc0001469.1_g000009 Rmu_sc0002111.1_g000036 Rmu_sc0002260.1_g000046 Rmu_sc0002260.1_g000047 Rmu_sc0002260.1_g000064 Rmu_sc0002260.1_g000067 Rmu_sc0002260.1_g000068 Rmu_sc0002260.1_g000069 Rmu_sc0002260.1_g000075 Rmu_sc0002260.1_g000078 Rmu_sc0002260.1_g000079 Rmu_sc0002260.1_g000080 Rmu_sc0002260.1_g000088 Rmu_sc0002260.1_g000090 Rmu_sc0002260.1_g000093 Rmu_sc0002260.1_g000094 Rmu_sc0002260.1_g000096 Rmu_sc0004705.1_g000030 Rmu_sc0006329.1_g000002 Rmu_sc0008373.1_g000005 Rmu_sc0008373.1_g000006 Rmu_sc0008373.1_g000007 Rmu_sc0008373.1_g000009 Rmu_sc0008373.1_g000010 Rmu_sc0013971.1_g000005 Rmu_sc0028704.1_g000001 Rmu_ssc0000420.1_g000042 Rmu_ssc0000420.1_g000043
rosa_roxburghii Rroxscaffold_1G00039420 Rroxscaffold_3G00255320 Rroxscaffold_5G00354650 Rroxscaffold_5G00354670 Rroxscaffold_5G00354680 Rroxscaffold_5G00354770 Rroxscaffold_5G00354780 Rroxscaffold_5G00354840 Rroxscaffold_5G00354850 Rroxscaffold_5G00354880 Rroxscaffold_5G00355060 Rroxscaffold_5G00365850 Rroxscaffold_5G00365860 Rroxscaffold_5G00365880 Rroxscaffold_5G00365980 Rroxscaffold_5G00365990 Rroxscaffold_5G00366000 Rroxscaffold_5G00366010 Rroxscaffold_5G00366030 Rroxscaffold_5G00366040 Rroxscaffold_5G00366110 Rroxscaffold_5G00366120
rosa_rugosa Rorug04G0100900 Rorug04G0100900 Rorug04G0101000 Rorug04G0101000 Rorug04G0101100 Rorug04G0101600 Rorug04G0101700 Rorug04G0101700 Rorug04G0101900 Rorug04G0189300 Rorug04G0189400 Rorug04G0189500 Rorug04G0189500 Rorug04G0189600 Rorug04G0191600 Rorug04G0191800 Rorug04G0191800 Rorug04G0191900 Rorug04G0192000 Rorug04G0192100 Rorug04G0192200 Rorug04G0192300 Rorug04G0192500 Rorug04G0192500 Rorug04G0192600 Rorug04G0192700 Rorug04G0192700 Rorug04G0192700 Rorug04G0193200 Rorug04G0193300 Rorug04G0201800 Rorug04G0201800 Rorug07G0063300.1
rosa_samantha Rh4CG170500 Rh4CG171600 Rh4CG171900 Rh4CG262200 Rh4CG262400 Rh4CG262500 Rh4CG262600 Rh4CG262700 Rh4CG262800 Rh4CG262900 Rh4CG263000 Rh4CG264700 Rh4CG264800 Rh4CG264900 Rh4CG265000 Rh4CG265100 Rh4CG265200 Rh4CG265300 Rh4CG265500 Rh4CG265600 Rh4CG265700 Rh4CG265800 Rh4CG265900 Rh4CG266100 Rh4CG266200 Rh4CG266800 Rh4CG267200 Rh4CG275400 Rh4CG275500 Rh7CG201600
rosa_wichuraiana Rw4G013200 Rw4G013210 Rw4G013220 Rw4G013300 Rw4G021330 Rw4G021340 Rw4G021350 Rw4G021360 Rw4G021370 Rw4G021380 Rw4G021510 Rw4G021530 Rw4G021540 Rw4G021560 Rw4G021570 Rw4G021580 Rw4G021590 Rw4G021600 Rw4G021610 Rw4G021620 Rw4G021630 Rw4G021650 Rw4G021660 Rw4G022390 Rw7G016700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 24
AasI GACNNNNNNGTC 1 cut(s) 60
AccI GTMKAC 1 cut(s) 444
AciI CCGC 2 cut(s) 324, 348
AclWI GGATC 2 cut(s) 325, 409
AfaI GTAC 2 cut(s) 401, 563
AfiI CCNNNNNNNGG 2 cut(s) 533, 571
AgsI TTSAA 4 cut(s) 40, 163, 262, 637
AjiI CACGTC 2 cut(s) 442, 601
AluBI AGCT 4 cut(s) 140, 450, 524, 640
AluI AGCT 4 cut(s) 140, 450, 524, 640
Alw21I GWGCWC 2 cut(s) 554, 600
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 2 cut(s) 325, 409
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 4 cut(s) 264, 349, 606, 621
ApeKI GCWGC 2 cut(s) 387, 521
AspA2I CCTAGG 1 cut(s) 251
AspS9I GGNCC 2 cut(s) 350, 661
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 3 cut(s) 29, 223, 587
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 1 cut(s) 661
AvrII CCTAGG 1 cut(s) 251
AxyI CCTNAGG 1 cut(s) 309
Bbv12I GWGCWC 2 cut(s) 554, 600
BbvI GCAGC 2 cut(s) 399, 533
BccI CCATC 4 cut(s) 160, 338, 494, 548
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciVI GTATCC 1 cut(s) 644
BclI TGATCA 1 cut(s) 195
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 2 cut(s) 445, 451
BfuI GTATCC 1 cut(s) 644
BisI GCNGC 3 cut(s) 349, 388, 522
BlnI CCTAGG 1 cut(s) 251
BlsI GCNGC 3 cut(s) 350, 389, 523
Bme1390I CCNGG 1 cut(s) 354
Bme18I GGWCC 1 cut(s) 661
BmeT110I CYCGRG 1 cut(s) 72
BmgBI CACGTC 2 cut(s) 442, 601
BmgT120I GGNCC 2 cut(s) 350, 661
BmiI GGNNCC 3 cut(s) 352, 358, 538
BmrFI CCNGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 268, 460
BplI GAGNNNNNCTC 4 cut(s) 218, 250, 608, 640
BpuEI CTTGAG 3 cut(s) 171, 347, 641
BpuMI CCSGG 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 403
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 3 cut(s) 251, 352, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 571
Bse118I RCCGGY 1 cut(s) 608
Bse21I CCTNAGG 1 cut(s) 309
BseDI CCNNGG 3 cut(s) 251, 352, 570
BseGI GGATG 3 cut(s) 155, 240, 291
BseLI CCNNNNNNNGG 2 cut(s) 533, 571
BseMII CTCAG 2 cut(s) 318, 323
BseRI GAGGAG 1 cut(s) 516
BseXI GCAGC 2 cut(s) 399, 533
BseYI CCCAGC 1 cut(s) 668
BshFI GGCC 4 cut(s) 266, 351, 608, 623
BsiHKAI GWGCWC 2 cut(s) 554, 600
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 2 cut(s) 354, 609
BslI CCNNNNNNNGG 2 cut(s) 533, 571
BsmAI GTCTC 1 cut(s) 500
BsnI GGCC 4 cut(s) 266, 351, 608, 623
Bso31I GGTCTC 1 cut(s) 500
BsoBI CYCGRG 1 cut(s) 72
Bsp1286I GDGCHC 2 cut(s) 554, 600
Bsp143I GATC 5 cut(s) 195, 330, 369, 414, 495
BspACI CCGC 2 cut(s) 324, 348
BspANI GGCC 4 cut(s) 266, 351, 608, 623
BspCNI CTCAG 2 cut(s) 319, 322
BspLI GGNNCC 3 cut(s) 352, 358, 538
BspPI GGATC 2 cut(s) 325, 409
BspQI GCTCTTC 1 cut(s) 588
BspTNI GGTCTC 1 cut(s) 500
BsrFI RCCGGY 1 cut(s) 608
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 3 cut(s) 251, 352, 570
BssMI GATC 5 cut(s) 195, 330, 369, 414, 495
BssT1I CCWWGG 2 cut(s) 251, 570
Bst4CI ACNGT 2 cut(s) 57, 455
Bst6I CTCTTC 2 cut(s) 588, 638
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 610
BstDEI CTNAG 2 cut(s) 309, 327
BstENI CCTNNNNNAGG 1 cut(s) 531
BstF5I GGATG 3 cut(s) 155, 240, 291
BstKTI GATC 5 cut(s) 198, 333, 372, 417, 498
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 5 cut(s) 195, 330, 369, 414, 495
BstMWI GCNNNNNNNGC 2 cut(s) 311, 387
BstSCI CCNGG 1 cut(s) 352
BstSFI CTRYAG 2 cut(s) 445, 451
BstV1I GCAGC 2 cut(s) 399, 533
BstX2I RGATCY 2 cut(s) 330, 414
BstXI CCANNNNNNTGG 1 cut(s) 517
BstYI RGATCY 2 cut(s) 330, 414
Bsu36I CCTNAGG 1 cut(s) 309
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 4 cut(s) 266, 351, 608, 623
BtrI CACGTC 2 cut(s) 442, 601
BtsCI GGATG 3 cut(s) 155, 240, 291
Cac8I GCNNGC 1 cut(s) 610
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 2 cut(s) 350, 661
Csp6I GTAC 2 cut(s) 400, 562
CspCI CAANNNNNGTGG 2 cut(s) 237, 272
CviAII CATG 1 cut(s) 202
CviQI GTAC 2 cut(s) 400, 562
DdeI CTNAG 2 cut(s) 309, 327
DpnI GATC 5 cut(s) 197, 332, 371, 416, 497
DpnII GATC 5 cut(s) 195, 330, 369, 414, 495
DrdI GACNNNNNNGTC 1 cut(s) 60
DseDI GACNNNNNNGTC 1 cut(s) 60
Eam1104I CTCTTC 2 cut(s) 588, 638
EarI CTCTTC 2 cut(s) 588, 638
Eco130I CCWWGG 2 cut(s) 251, 570
Eco147I AGGCCT 2 cut(s) 266, 623
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 661
Eco81I CCTNAGG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 72
EcoNI CCTNNNNNAGG 1 cut(s) 531
EcoT14I CCWWGG 2 cut(s) 251, 570
ErhI CCWWGG 2 cut(s) 251, 570
FaeI CATG 1 cut(s) 205
FaiI YATR 6 cut(s) 24, 50, 99, 203, 219, 504
FalI AAGNNNNNCTT 2 cut(s) 93, 125
FatI CATG 1 cut(s) 201
FbaI TGATCA 1 cut(s) 195
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 3 cut(s) 349, 388, 522
FokI GGATG 3 cut(s) 142, 227, 278
Fsp4HI GCNGC 3 cut(s) 349, 388, 522
FspBI CTAG 1 cut(s) 252
GluI GCNGC 3 cut(s) 349, 388, 522
GsaI CCCAGC 1 cut(s) 672
HaeIII GGCC 4 cut(s) 266, 351, 608, 623
HapII CCGG 2 cut(s) 354, 609
Hin1II CATG 1 cut(s) 205
HpaII CCGG 2 cut(s) 354, 609
HphI GGTGA 3 cut(s) 29, 223, 587
Hpy166II GTNNAC 4 cut(s) 69, 211, 231, 445
Hpy188I TCNGA 2 cut(s) 238, 481
Hpy188III TCNNGA 6 cut(s) 74, 193, 275, 364, 490, 547
Hpy8I GTNNAC 4 cut(s) 69, 211, 231, 445
HpyAV CCTTC 5 cut(s) 119, 277, 332, 377, 613
HpyCH4III ACNGT 2 cut(s) 57, 455
HpyCH4IV ACGT 3 cut(s) 402, 441, 600
HpyCH4V TGCA 1 cut(s) 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 387
HpyF3I CTNAG 2 cut(s) 309, 327
HpySE526I ACGT 3 cut(s) 402, 441, 600
Hsp92II CATG 1 cut(s) 205
KroI GCCGGC 1 cut(s) 608
KroNI GCCGGC 1 cut(s) 610
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 5 cut(s) 195, 330, 369, 414, 495
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 2 cut(s) 145, 529
LpnPI CCDG 4 cut(s) 296, 367, 568, 622
Lsp1109I GCAGC 2 cut(s) 399, 533
LweI GCATC 2 cut(s) 268, 460
MaeI CTAG 1 cut(s) 252
MaeII ACGT 3 cut(s) 402, 441, 600
MaeIII GTNAC 1 cut(s) 115
MalI GATC 5 cut(s) 197, 332, 371, 416, 497
MboI GATC 5 cut(s) 195, 330, 369, 414, 495
MboII GAAGA 6 cut(s) 73, 161, 379, 505, 605, 625
MflI RGATCY 2 cut(s) 330, 414
MhlI GDGCHC 2 cut(s) 554, 600
MmeI TCCRAC 2 cut(s) 151, 261
MroNI GCCGGC 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 2 cut(s) 354, 609
MspR9I CCNGG 1 cut(s) 354
MwoI GCNNNNNNNGC 2 cut(s) 311, 387
NaeI GCCGGC 1 cut(s) 610
NciI CCSGG 1 cut(s) 354
NdeII GATC 5 cut(s) 195, 330, 369, 414, 495
NgoMIV GCCGGC 1 cut(s) 608
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 3 cut(s) 352, 358, 538
PceI AGGCCT 2 cut(s) 266, 623
PciSI GCTCTTC 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PdiI GCCGGC 1 cut(s) 610
PkrI GCNGC 3 cut(s) 350, 389, 523
Ppu21I YACGTR 1 cut(s) 403
PsiI TTATAA 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 668
PspN4I GGNNCC 3 cut(s) 352, 358, 538
PspPI GGNCC 2 cut(s) 350, 661
PsrI GAACNNNNNNTAC 4 cut(s) 50, 82, 428, 460
PsuI RGATCY 2 cut(s) 330, 414
RsaI GTAC 2 cut(s) 401, 563
RsaNI GTAC 2 cut(s) 400, 562
SapI GCTCTTC 1 cut(s) 588
SatI GCNGC 3 cut(s) 349, 388, 522
Sau3AI GATC 5 cut(s) 195, 330, 369, 414, 495
Sau96I GGNCC 2 cut(s) 350, 661
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 2 cut(s) 554, 600
SfaNI GCATC 2 cut(s) 268, 460
SfcI CTRYAG 2 cut(s) 445, 451
SinI GGWCC 1 cut(s) 661
SmlI CTYRAG 3 cut(s) 186, 362, 656
SmoI CTYRAG 3 cut(s) 186, 362, 656
SseBI AGGCCT 2 cut(s) 266, 623
SsiI CCGC 2 cut(s) 324, 348
SspMI CTAG 1 cut(s) 252
StuI AGGCCT 2 cut(s) 266, 623
StyD4I CCNGG 1 cut(s) 352
StyI CCWWGG 2 cut(s) 251, 570
TaaI ACNGT 2 cut(s) 57, 455
TaiI ACGT 3 cut(s) 405, 444, 603
TaqI TCGA 5 cut(s) 336, 462, 489, 498, 582
TauI GCSGC 1 cut(s) 351
TseI GCWGC 2 cut(s) 387, 521
TspDTI ATGAA 1 cut(s) 234
TspGWI ACGGA 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 661
XagI CCTNNNNNAGG 1 cut(s) 531
XmaJI CCTAGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 444
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.