Major allergen Pru
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 326 |
| AccB1I | GGYRCC | 1 cut(s) | 326 |
| AciI | CCGC | 1 cut(s) | 123 |
| AclWI | GGATC | 1 cut(s) | 124 |
| AfaI | GTAC | 2 cut(s) | 17, 328 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 51, 361, 427 |
| AgsI | TTSAA | 4 cut(s) | 13, 61, 137, 427 |
| AjiI | CACGTC | 1 cut(s) | 391 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 431, 463 |
| AluBI | AGCT | 3 cut(s) | 50, 198, 269 |
| AluI | AGCT | 3 cut(s) | 50, 198, 269 |
| Alw21I | GWGCWC | 4 cut(s) | 271, 344, 390, 456 |
| Alw26I | GTCTC | 1 cut(s) | 293 |
| AlwI | GGATC | 1 cut(s) | 124 |
| AoxI | GGCC | 1 cut(s) | 396 |
| Asp718I | GGTACC | 1 cut(s) | 326 |
| AsuC2I | CCSGG | 1 cut(s) | 400 |
| AsuHPI | GGTGA | 3 cut(s) | 163, 377, 410 |
| BanI | GGYRCC | 1 cut(s) | 326 |
| BanII | GRGCYC | 1 cut(s) | 271 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 278, 310 |
| Bbv12I | GWGCWC | 4 cut(s) | 271, 344, 390, 456 |
| BccI | CCATC | 3 cut(s) | 137, 167, 338 |
| BcnI | CCSGG | 1 cut(s) | 400 |
| BcoDI | GTCTC | 1 cut(s) | 293 |
| BfaI | CTAG | 1 cut(s) | 51 |
| BfmI | CTRYAG | 1 cut(s) | 250 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 110 |
| BglII | AGATCT | 1 cut(s) | 288 |
| Bme1390I | CCNGG | 1 cut(s) | 400 |
| BmgBI | CACGTC | 1 cut(s) | 391 |
| BmiI | GGNNCC | 2 cut(s) | 157, 328 |
| BmrFI | CCNGG | 1 cut(s) | 400 |
| BmsI | GCATC | 3 cut(s) | 67, 323, 451 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 17, 49 |
| BpuMI | CCSGG | 1 cut(s) | 400 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 51, 361, 427 |
| Bse1I | ACTGG | 1 cut(s) | 345 |
| BseGI | GGATG | 3 cut(s) | 39, 90, 314 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 51, 361, 427 |
| BseMII | CTCAG | 1 cut(s) | 117 |
| BseNI | ACTGG | 1 cut(s) | 345 |
| BseYI | CCCAGC | 1 cut(s) | 46 |
| BshFI | GGCC | 1 cut(s) | 398 |
| BshNI | GGYRCC | 1 cut(s) | 326 |
| BsiHKAI | GWGCWC | 4 cut(s) | 271, 344, 390, 456 |
| BsiSI | CCGG | 2 cut(s) | 318, 399 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 51, 361, 427 |
| BsmAI | GTCTC | 1 cut(s) | 293 |
| BsnI | GGCC | 1 cut(s) | 398 |
| Bsp1286I | GDGCHC | 4 cut(s) | 271, 344, 390, 456 |
| Bsp143I | GATC | 4 cut(s) | 129, 168, 288, 375 |
| BspACI | CCGC | 1 cut(s) | 123 |
| BspANI | GGCC | 1 cut(s) | 398 |
| BspCNI | CTCAG | 1 cut(s) | 118 |
| BspLI | GGNNCC | 2 cut(s) | 157, 328 |
| BspPI | GGATC | 1 cut(s) | 124 |
| BspQI | GCTCTTC | 1 cut(s) | 378 |
| BspT107I | GGYRCC | 1 cut(s) | 326 |
| BsrI | ACTGG | 1 cut(s) | 345 |
| BssMI | GATC | 4 cut(s) | 129, 168, 288, 375 |
| Bst6I | CTCTTC | 2 cut(s) | 378, 428 |
| BstDEI | CTNAG | 2 cut(s) | 126, 303 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 425 |
| BstF5I | GGATG | 3 cut(s) | 39, 90, 314 |
| BstKTI | GATC | 4 cut(s) | 132, 171, 291, 378 |
| BstMAI | GTCTC | 1 cut(s) | 293 |
| BstMBI | GATC | 4 cut(s) | 129, 168, 288, 375 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 110, 186, 195 |
| BstSCI | CCNGG | 1 cut(s) | 398 |
| BstSFI | CTRYAG | 1 cut(s) | 250 |
| BstX2I | RGATCY | 2 cut(s) | 129, 288 |
| BstYI | RGATCY | 2 cut(s) | 129, 288 |
| BsuRI | GGCC | 1 cut(s) | 398 |
| BtrI | CACGTC | 1 cut(s) | 391 |
| BtsCI | GGATG | 3 cut(s) | 39, 90, 314 |
| BtsI | GCAGTG | 1 cut(s) | 328 |
| BtsIMutI | CAGTG | 2 cut(s) | 328, 352 |
| Csp6I | GTAC | 2 cut(s) | 16, 327 |
| CviAII | CATG | 2 cut(s) | 356, 458 |
| CviJI | RGCY | 6 cut(s) | 50, 65, 198, 269, 398, 413 |
| CviKI_1 | RGCY | 6 cut(s) | 50, 65, 198, 269, 398, 413 |
| CviQI | GTAC | 2 cut(s) | 16, 327 |
| DdeI | CTNAG | 2 cut(s) | 126, 303 |
| DpnI | GATC | 4 cut(s) | 131, 170, 290, 377 |
| DpnII | GATC | 4 cut(s) | 129, 168, 288, 375 |
| Eam1104I | CTCTTC | 2 cut(s) | 378, 428 |
| EarI | CTCTTC | 2 cut(s) | 378, 428 |
| Ecl136II | GAGCTC | 1 cut(s) | 269 |
| Eco24I | GRGCYC | 1 cut(s) | 271 |
| Eco53kI | GAGCTC | 1 cut(s) | 269 |
| EcoICRI | GAGCTC | 1 cut(s) | 269 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 425 |
| EcoT38I | GRGCYC | 1 cut(s) | 271 |
| FaeI | CATG | 2 cut(s) | 359, 461 |
| FaiI | YATR | 4 cut(s) | 201, 252, 357, 459 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 158, 190, 221, 253 |
| FatI | CATG | 2 cut(s) | 355, 457 |
| FokI | GGATG | 3 cut(s) | 26, 77, 301 |
| FriOI | GRGCYC | 1 cut(s) | 271 |
| FspBI | CTAG | 1 cut(s) | 51 |
| GsaI | CCCAGC | 1 cut(s) | 50 |
| HaeIII | GGCC | 1 cut(s) | 398 |
| HapII | CCGG | 2 cut(s) | 318, 399 |
| Hin1II | CATG | 2 cut(s) | 359, 461 |
| HinfI | GANTC | 2 cut(s) | 20, 275 |
| HpaII | CCGG | 2 cut(s) | 318, 399 |
| HphI | GGTGA | 3 cut(s) | 163, 377, 410 |
| Hpy166II | GTNNAC | 1 cut(s) | 30 |
| Hpy188I | TCNGA | 1 cut(s) | 25 |
| Hpy188III | TCNNGA | 4 cut(s) | 74, 163, 272, 337 |
| Hpy8I | GTNNAC | 1 cut(s) | 30 |
| HpyAV | CCTTC | 4 cut(s) | 131, 176, 184, 403 |
| HpyCH4IV | ACGT | 1 cut(s) | 390 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 110, 186, 195 |
| HpyF3I | CTNAG | 2 cut(s) | 126, 303 |
| HpySE526I | ACGT | 1 cut(s) | 390 |
| Hsp92II | CATG | 2 cut(s) | 359, 461 |
| KpnI | GGTACC | 1 cut(s) | 330 |
| Kzo9I | GATC | 4 cut(s) | 129, 168, 288, 375 |
| LguI | GCTCTTC | 1 cut(s) | 378 |
| LmnI | GCTCC | 3 cut(s) | 266, 274, 451 |
| LpnPI | CCDG | 6 cut(s) | 60, 95, 138, 331, 358, 412 |
| LweI | GCATC | 3 cut(s) | 67, 323, 451 |
| MaeI | CTAG | 1 cut(s) | 51 |
| MaeII | ACGT | 1 cut(s) | 390 |
| MalI | GATC | 4 cut(s) | 131, 170, 290, 377 |
| MboI | GATC | 4 cut(s) | 129, 168, 288, 375 |
| MboII | GAAGA | 4 cut(s) | 178, 298, 395, 415 |
| MfeI | CAATTG | 1 cut(s) | 279 |
| MflI | RGATCY | 2 cut(s) | 129, 288 |
| MhlI | GDGCHC | 4 cut(s) | 271, 344, 390, 456 |
| MluCI | AATT | 2 cut(s) | 213, 279 |
| MlyI | GAGTC | 2 cut(s) | 29, 269 |
| MnlI | CCTC | 5 cut(s) | 43, 98, 256, 431, 455 |
| MseI | TTAA | 1 cut(s) | 117 |
| MslI | CAYNNNNRTG | 1 cut(s) | 459 |
| MspA1I | CMGCKG | 1 cut(s) | 125 |
| MspI | CCGG | 2 cut(s) | 318, 399 |
| MspR9I | CCNGG | 1 cut(s) | 400 |
| MunI | CAATTG | 1 cut(s) | 279 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 110, 186, 195 |
| NciI | CCSGG | 1 cut(s) | 400 |
| NdeII | GATC | 4 cut(s) | 129, 168, 288, 375 |
| NlaIII | CATG | 2 cut(s) | 359, 461 |
| NlaIV | GGNNCC | 2 cut(s) | 157, 328 |
| PciSI | GCTCTTC | 1 cut(s) | 378 |
| PleI | GAGTC | 2 cut(s) | 28, 269 |
| PpsI | GAGTC | 2 cut(s) | 28, 269 |
| Psp124BI | GAGCTC | 1 cut(s) | 271 |
| PspFI | CCCAGC | 1 cut(s) | 46 |
| PspN4I | GGNNCC | 2 cut(s) | 157, 328 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 227, 259 |
| PsuI | RGATCY | 2 cut(s) | 129, 288 |
| RsaI | GTAC | 2 cut(s) | 17, 328 |
| RsaNI | GTAC | 2 cut(s) | 16, 327 |
| RseI | CAYNNNNRTG | 1 cut(s) | 459 |
| SacI | GAGCTC | 1 cut(s) | 271 |
| SapI | GCTCTTC | 1 cut(s) | 378 |
| SaqAI | TTAA | 1 cut(s) | 117 |
| Sau3AI | GATC | 4 cut(s) | 129, 168, 288, 375 |
| SchI | GAGTC | 2 cut(s) | 29, 269 |
| ScrFI | CCNGG | 1 cut(s) | 400 |
| SduI | GDGCHC | 4 cut(s) | 271, 344, 390, 456 |
| SfaNI | GCATC | 3 cut(s) | 67, 323, 451 |
| SfcI | CTRYAG | 1 cut(s) | 250 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 459 |
| Sse9I | AATT | 2 cut(s) | 213, 279 |
| SsiI | CCGC | 1 cut(s) | 123 |
| SspMI | CTAG | 1 cut(s) | 51 |
| SstI | GAGCTC | 1 cut(s) | 271 |
| StyD4I | CCNGG | 1 cut(s) | 398 |
| TaiI | ACGT | 1 cut(s) | 393 |
| TaqI | TCGA | 1 cut(s) | 75 |
| TasI | AATT | 2 cut(s) | 213, 279 |
| Tru1I | TTAA | 1 cut(s) | 117 |
| Tru9I | TTAA | 1 cut(s) | 117 |
| TscAI | CASTG | 2 cut(s) | 328, 352 |
| TspRI | CASTG | 2 cut(s) | 328, 352 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 425 |
| XspI | CTAG | 1 cut(s) | 51 |