Major allergen Pru
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 224 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 300 |
| AcsI | RAATTY | 1 cut(s) | 155 |
| AcuI | CTGAAG | 1 cut(s) | 464 |
| AcyI | GRCGYC | 1 cut(s) | 225 |
| AfaI | GTAC | 1 cut(s) | 299 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 192, 300 |
| AgsI | TTSAA | 2 cut(s) | 383, 478 |
| AjnI | CCWGG | 1 cut(s) | 185 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 159, 191 |
| AluBI | AGCT | 2 cut(s) | 326, 386 |
| AluI | AGCT | 2 cut(s) | 326, 386 |
| Alw26I | GTCTC | 1 cut(s) | 370 |
| AoxI | GGCC | 3 cut(s) | 47, 87, 432 |
| ApoI | RAATTY | 1 cut(s) | 155 |
| AsiSI | GCGATCGC | 1 cut(s) | 413 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 171, 453 |
| AspLEI | GCGC | 1 cut(s) | 227 |
| AspS9I | GGNCC | 2 cut(s) | 87, 291 |
| AsuC2I | CCSGG | 1 cut(s) | 436 |
| AsuHPI | GGTGA | 1 cut(s) | 281 |
| AvaII | GGWCC | 1 cut(s) | 291 |
| AxyI | CCTNAGG | 1 cut(s) | 44 |
| BanI | GGYRCC | 1 cut(s) | 224 |
| BbsI | GAAGAC | 1 cut(s) | 503 |
| BccI | CCATC | 1 cut(s) | 140 |
| BciT130I | CCWGG | 1 cut(s) | 187 |
| BcnI | CCSGG | 1 cut(s) | 436 |
| BcoDI | GTCTC | 1 cut(s) | 370 |
| BfaI | CTAG | 2 cut(s) | 15, 206 |
| BfmI | CTRYAG | 1 cut(s) | 519 |
| BfoI | RGCGCY | 1 cut(s) | 228 |
| BmcAI | AGTACT | 1 cut(s) | 299 |
| Bme1390I | CCNGG | 2 cut(s) | 187, 436 |
| Bme18I | GGWCC | 1 cut(s) | 291 |
| BmgT120I | GGNCC | 2 cut(s) | 87, 291 |
| BmiI | GGNNCC | 4 cut(s) | 126, 226, 292, 440 |
| BmrFI | CCNGG | 2 cut(s) | 187, 436 |
| BmrI | ACTGGG | 1 cut(s) | 310 |
| BmuI | ACTGGG | 1 cut(s) | 310 |
| BpiI | GAAGAC | 1 cut(s) | 503 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 258, 290 |
| BpmI | CTGGAG | 1 cut(s) | 446 |
| BpuMI | CCSGG | 1 cut(s) | 436 |
| Bsa29I | ATCGAT | 2 cut(s) | 69, 526 |
| BsaHI | GRCGYC | 1 cut(s) | 225 |
| BsaJI | CCNNGG | 1 cut(s) | 185 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 159, 189 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 192, 300 |
| Bse1I | ACTGG | 1 cut(s) | 305 |
| Bse21I | CCTNAGG | 1 cut(s) | 44 |
| BseBI | CCWGG | 1 cut(s) | 187 |
| BseCI | ATCGAT | 2 cut(s) | 69, 526 |
| BseDI | CCNNGG | 1 cut(s) | 185 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 192, 300 |
| BseMII | CTCAG | 2 cut(s) | 35, 472 |
| BseNI | ACTGG | 1 cut(s) | 305 |
| BsgI | GTGCAG | 1 cut(s) | 100 |
| Bsh1285I | CGRYCG | 1 cut(s) | 413 |
| BshFI | GGCC | 3 cut(s) | 49, 89, 434 |
| BshNI | GGYRCC | 1 cut(s) | 224 |
| BshVI | ATCGAT | 2 cut(s) | 69, 526 |
| BsiEI | CGRYCG | 1 cut(s) | 413 |
| BsiSI | CCGG | 1 cut(s) | 435 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 192, 300 |
| BsmAI | GTCTC | 1 cut(s) | 370 |
| BsnI | GGCC | 3 cut(s) | 49, 89, 434 |
| Bsp143I | GATC | 4 cut(s) | 36, 256, 410, 523 |
| BspANI | GGCC | 3 cut(s) | 49, 89, 434 |
| BspCNI | CTCAG | 2 cut(s) | 36, 471 |
| BspDI | ATCGAT | 2 cut(s) | 69, 526 |
| BspLI | GGNNCC | 4 cut(s) | 126, 226, 292, 440 |
| BspT107I | GGYRCC | 1 cut(s) | 224 |
| BsrI | ACTGG | 1 cut(s) | 305 |
| BssECI | CCNNGG | 1 cut(s) | 185 |
| BssMI | GATC | 4 cut(s) | 36, 256, 410, 523 |
| BssNI | GRCGYC | 1 cut(s) | 225 |
| Bst2UI | CCWGG | 1 cut(s) | 187 |
| BstACI | GRCGYC | 1 cut(s) | 225 |
| BstDEI | CTNAG | 2 cut(s) | 44, 458 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 190 |
| BstH2I | RGCGCY | 1 cut(s) | 228 |
| BstHHI | GCGC | 1 cut(s) | 227 |
| BstKTI | GATC | 4 cut(s) | 39, 259, 413, 526 |
| BstMAI | GTCTC | 1 cut(s) | 370 |
| BstMBI | GATC | 4 cut(s) | 36, 256, 410, 523 |
| BstMCI | CGRYCG | 1 cut(s) | 413 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 323 |
| BstNI | CCWGG | 1 cut(s) | 187 |
| BstSCI | CCNGG | 2 cut(s) | 185, 434 |
| BstSFI | CTRYAG | 1 cut(s) | 519 |
| BstV2I | GAAGAC | 1 cut(s) | 503 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 186 |
| Bsu15I | ATCGAT | 2 cut(s) | 69, 526 |
| Bsu36I | CCTNAGG | 1 cut(s) | 44 |
| BsuRI | GGCC | 3 cut(s) | 49, 89, 434 |
| BsuTUI | ATCGAT | 2 cut(s) | 69, 526 |
| BtsI | GCAGTG | 1 cut(s) | 25 |
| BtsIMutI | CAGTG | 2 cut(s) | 25, 90 |
| CfoI | GCGC | 1 cut(s) | 227 |
| Cfr13I | GGNCC | 2 cut(s) | 87, 291 |
| ClaI | ATCGAT | 2 cut(s) | 69, 526 |
| Csp6I | GTAC | 1 cut(s) | 298 |
| CviAII | CATG | 1 cut(s) | 79 |
| CviQI | GTAC | 1 cut(s) | 298 |
| DdeI | CTNAG | 2 cut(s) | 44, 458 |
| DinI | GGCGCC | 1 cut(s) | 226 |
| DpnI | GATC | 4 cut(s) | 38, 258, 412, 525 |
| DpnII | GATC | 4 cut(s) | 36, 256, 410, 523 |
| DraI | TTTAAA | 1 cut(s) | 355 |
| Eco47I | GGWCC | 1 cut(s) | 291 |
| Eco57I | CTGAAG | 1 cut(s) | 464 |
| Eco81I | CCTNAGG | 1 cut(s) | 44 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 190 |
| EcoRII | CCWGG | 1 cut(s) | 185 |
| EgeI | GGCGCC | 1 cut(s) | 226 |
| EheI | GGCGCC | 1 cut(s) | 226 |
| FaeI | CATG | 1 cut(s) | 82 |
| FaiI | YATR | 6 cut(s) | 80, 282, 350, 468, 497, 507 |
| FatI | CATG | 1 cut(s) | 78 |
| FspBI | CTAG | 2 cut(s) | 15, 206 |
| GlaI | GCGC | 1 cut(s) | 226 |
| GsuI | CTGGAG | 1 cut(s) | 446 |
| HaeII | RGCGCY | 1 cut(s) | 228 |
| HaeIII | GGCC | 3 cut(s) | 49, 89, 434 |
| HapII | CCGG | 1 cut(s) | 435 |
| HhaI | GCGC | 1 cut(s) | 227 |
| Hin1I | GRCGYC | 1 cut(s) | 225 |
| Hin1II | CATG | 1 cut(s) | 82 |
| Hin6I | GCGC | 1 cut(s) | 225 |
| HinP1I | GCGC | 1 cut(s) | 225 |
| HinfI | GANTC | 1 cut(s) | 364 |
| HpaII | CCGG | 1 cut(s) | 435 |
| HphI | GGTGA | 1 cut(s) | 281 |
| Hpy166II | GTNNAC | 2 cut(s) | 272, 400 |
| Hpy188I | TCNGA | 2 cut(s) | 64, 461 |
| Hpy188III | TCNNGA | 2 cut(s) | 425, 442 |
| Hpy8I | GTNNAC | 2 cut(s) | 272, 400 |
| HpyAV | CCTTC | 4 cut(s) | 424, 439, 443, 472 |
| HpyCH4V | TGCA | 2 cut(s) | 117, 317 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 323 |
| HpyF3I | CTNAG | 2 cut(s) | 44, 458 |
| Hsp92I | GRCGYC | 1 cut(s) | 225 |
| Hsp92II | CATG | 1 cut(s) | 82 |
| HspAI | GCGC | 1 cut(s) | 225 |
| KasI | GGCGCC | 1 cut(s) | 224 |
| Kzo9I | GATC | 4 cut(s) | 36, 256, 410, 523 |
| LmnI | GCTCC | 1 cut(s) | 124 |
| MaeI | CTAG | 2 cut(s) | 15, 206 |
| MalI | GATC | 4 cut(s) | 38, 258, 412, 525 |
| MboI | GATC | 4 cut(s) | 36, 256, 410, 523 |
| MboII | GAAGA | 3 cut(s) | 155, 495, 503 |
| MfeI | CAATTG | 1 cut(s) | 219 |
| MluCI | AATT | 2 cut(s) | 155, 219 |
| Mly113I | GGCGCC | 1 cut(s) | 225 |
| MlyI | GAGTC | 1 cut(s) | 358 |
| MnlI | CCTC | 5 cut(s) | 39, 117, 250, 260, 411 |
| MroXI | GAANNNNTTC | 2 cut(s) | 171, 453 |
| MseI | TTAA | 2 cut(s) | 153, 354 |
| MslI | CAYNNNNRTG | 1 cut(s) | 233 |
| MspI | CCGG | 1 cut(s) | 435 |
| MspR9I | CCNGG | 2 cut(s) | 187, 436 |
| MunI | CAATTG | 1 cut(s) | 219 |
| MvaI | CCWGG | 1 cut(s) | 187 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 323 |
| NarI | GGCGCC | 1 cut(s) | 225 |
| NciI | CCSGG | 1 cut(s) | 436 |
| NdeII | GATC | 4 cut(s) | 36, 256, 410, 523 |
| NlaIII | CATG | 1 cut(s) | 82 |
| NlaIV | GGNNCC | 4 cut(s) | 126, 226, 292, 440 |
| NmeAIII | GCCGAG | 1 cut(s) | 255 |
| PdmI | GAANNNNTTC | 2 cut(s) | 171, 453 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 300 |
| Ple19I | CGATCG | 1 cut(s) | 413 |
| PleI | GAGTC | 1 cut(s) | 358 |
| PluTI | GGCGCC | 1 cut(s) | 228 |
| PpsI | GAGTC | 1 cut(s) | 358 |
| Psp6I | CCWGG | 1 cut(s) | 185 |
| PspGI | CCWGG | 1 cut(s) | 185 |
| PspN4I | GGNNCC | 4 cut(s) | 126, 226, 292, 440 |
| PspPI | GGNCC | 2 cut(s) | 87, 291 |
| PvuI | CGATCG | 1 cut(s) | 413 |
| RgaI | GCGATCGC | 1 cut(s) | 413 |
| RsaI | GTAC | 1 cut(s) | 299 |
| RsaNI | GTAC | 1 cut(s) | 298 |
| RseI | CAYNNNNRTG | 1 cut(s) | 233 |
| SaqAI | TTAA | 2 cut(s) | 153, 354 |
| Sau3AI | GATC | 4 cut(s) | 36, 256, 410, 523 |
| Sau96I | GGNCC | 2 cut(s) | 87, 291 |
| ScaI | AGTACT | 1 cut(s) | 299 |
| SchI | GAGTC | 1 cut(s) | 358 |
| ScrFI | CCNGG | 2 cut(s) | 187, 436 |
| SetI | ASST | 5 cut(s) | 174, 271, 328, 388, 454 |
| SfaAI | GCGATCGC | 1 cut(s) | 413 |
| SfcI | CTRYAG | 1 cut(s) | 519 |
| SfoI | GGCGCC | 1 cut(s) | 226 |
| SgfI | GCGATCGC | 1 cut(s) | 413 |
| SinI | GGWCC | 1 cut(s) | 291 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 233 |
| Sse9I | AATT | 2 cut(s) | 155, 219 |
| SspDI | GGCGCC | 1 cut(s) | 224 |
| SspMI | CTAG | 2 cut(s) | 15, 206 |
| StyD4I | CCNGG | 2 cut(s) | 185, 434 |
| TaqI | TCGA | 2 cut(s) | 69, 526 |
| TasI | AATT | 2 cut(s) | 155, 219 |
| TatI | WGTACW | 1 cut(s) | 297 |
| Tru1I | TTAA | 2 cut(s) | 153, 354 |
| Tru9I | TTAA | 2 cut(s) | 153, 354 |
| TscAI | CASTG | 2 cut(s) | 25, 97 |
| TspDTI | ATGAA | 4 cut(s) | 67, 111, 148, 483 |
| TspRI | CASTG | 2 cut(s) | 25, 97 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 300 |
| VpaK11BI | GGWCC | 1 cut(s) | 291 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 190 |
| XapI | RAATTY | 1 cut(s) | 155 |
| XmnI | GAANNNNTTC | 2 cut(s) | 171, 453 |
| XspI | CTAG | 2 cut(s) | 15, 206 |
| ZrmI | AGTACT | 1 cut(s) | 299 |