Major allergen Pru
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 15 |
| AciI | CCGC | 4 cut(s) | 15, 38, 493, 750 |
| AclWI | GGATC | 2 cut(s) | 208, 549 |
| AcsI | RAATTY | 1 cut(s) | 206 |
| AcuI | CTGAAG | 1 cut(s) | 300 |
| AfaI | GTAC | 1 cut(s) | 532 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 655 |
| AgsI | TTSAA | 9 cut(s) | 91, 184, 303, 393, 408, 414, 576, 680, 742 |
| AjiI | CACGTC | 1 cut(s) | 755 |
| AluBI | AGCT | 7 cut(s) | 59, 320, 333, 451, 521, 592, 698 |
| AluI | AGCT | 7 cut(s) | 59, 320, 333, 451, 521, 592, 698 |
| Alw21I | GWGCWC | 1 cut(s) | 100 |
| Alw26I | GTCTC | 2 cut(s) | 11, 248 |
| AlwI | GGATC | 2 cut(s) | 208, 549 |
| ApeKI | GCWGC | 1 cut(s) | 695 |
| ApoI | RAATTY | 1 cut(s) | 206 |
| AsuHPI | GGTGA | 2 cut(s) | 611, 745 |
| AsuII | TTCGAA | 1 cut(s) | 130 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 138, 171 |
| Bbv12I | GWGCWC | 1 cut(s) | 100 |
| BbvI | GCAGC | 1 cut(s) | 707 |
| BccI | CCATC | 2 cut(s) | 202, 493 |
| BciVI | GTATCC | 1 cut(s) | 139 |
| BclI | TGATCA | 1 cut(s) | 118 |
| BcoDI | GTCTC | 2 cut(s) | 11, 248 |
| BfuI | GTATCC | 1 cut(s) | 139 |
| BisI | GCNGC | 1 cut(s) | 696 |
| BlpI | GCTNAGC | 2 cut(s) | 99, 522 |
| BlsI | GCNGC | 1 cut(s) | 697 |
| BmgBI | CACGTC | 1 cut(s) | 755 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 99, 522 |
| Bpu14I | TTCGAA | 1 cut(s) | 130 |
| BpuEI | CTTGAG | 3 cut(s) | 62, 608, 649 |
| BsaBI | GATNNNNATC | 1 cut(s) | 120 |
| BsaJI | CCNNGG | 1 cut(s) | 654 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 655 |
| Bse1I | ACTGG | 4 cut(s) | 217, 282, 372, 378 |
| Bse8I | GATNNNNATC | 1 cut(s) | 120 |
| BseDI | CCNNGG | 1 cut(s) | 654 |
| BseGI | GGATG | 1 cut(s) | 121 |
| BseJI | GATNNNNATC | 1 cut(s) | 120 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 655 |
| BseMII | CTCAG | 5 cut(s) | 22, 113, 180, 443, 718 |
| BseNI | ACTGG | 4 cut(s) | 217, 282, 372, 378 |
| BseRI | GAGGAG | 1 cut(s) | 90 |
| BseXI | GCAGC | 1 cut(s) | 707 |
| BseYI | CCCAGC | 1 cut(s) | 592 |
| BsgI | GTGCAG | 1 cut(s) | 176 |
| Bsh1236I | CGCG | 1 cut(s) | 15 |
| BsiHKAI | GWGCWC | 1 cut(s) | 100 |
| BslFI | GGGAC | 2 cut(s) | 31, 342 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 655 |
| BsmAI | GTCTC | 2 cut(s) | 11, 248 |
| BsmFI | GGGAC | 2 cut(s) | 31, 342 |
| Bsp119I | TTCGAA | 1 cut(s) | 130 |
| Bsp1286I | GDGCHC | 1 cut(s) | 100 |
| Bsp1407I | TGTACA | 1 cut(s) | 530 |
| Bsp143I | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 99, 522 |
| BspACI | CCGC | 4 cut(s) | 15, 38, 493, 750 |
| BspCNI | CTCAG | 5 cut(s) | 21, 112, 181, 444, 717 |
| BspFNI | CGCG | 1 cut(s) | 15 |
| BspHI | TCATGA | 2 cut(s) | 250, 268 |
| BspPI | GGATC | 2 cut(s) | 208, 549 |
| BspT104I | TTCGAA | 1 cut(s) | 130 |
| BsrGI | TGTACA | 1 cut(s) | 530 |
| BsrI | ACTGG | 4 cut(s) | 217, 282, 372, 378 |
| BssECI | CCNNGG | 1 cut(s) | 654 |
| BssMI | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| BssT1I | CCWWGG | 1 cut(s) | 654 |
| Bst4CI | ACNGT | 1 cut(s) | 151 |
| BstAUI | TGTACA | 1 cut(s) | 530 |
| BstBI | TTCGAA | 1 cut(s) | 130 |
| BstDEI | CTNAG | 7 cut(s) | 8, 99, 189, 452, 522, 548, 704 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 653 |
| BstF5I | GGATG | 1 cut(s) | 121 |
| BstFNI | CGCG | 1 cut(s) | 15 |
| BstKTI | GATC | 5 cut(s) | 121, 216, 310, 400, 544 |
| BstMAI | GTCTC | 2 cut(s) | 11, 248 |
| BstMBI | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 490 |
| BstUI | CGCG | 1 cut(s) | 15 |
| BstV1I | GCAGC | 1 cut(s) | 707 |
| BstX2I | RGATCY | 1 cut(s) | 541 |
| BstYI | RGATCY | 1 cut(s) | 541 |
| BsuI | GTATCC | 1 cut(s) | 139 |
| BtrI | CACGTC | 1 cut(s) | 755 |
| BtsCI | GGATG | 1 cut(s) | 121 |
| BtsIMutI | CAGTG | 2 cut(s) | 186, 385 |
| CciI | TCATGA | 2 cut(s) | 250, 268 |
| Csp6I | GTAC | 1 cut(s) | 531 |
| CviAII | CATG | 5 cut(s) | 110, 251, 269, 362, 528 |
| CviQI | GTAC | 1 cut(s) | 531 |
| DdeI | CTNAG | 7 cut(s) | 8, 99, 189, 452, 522, 548, 704 |
| DpnI | GATC | 5 cut(s) | 120, 215, 309, 399, 543 |
| DpnII | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| Eco130I | CCWWGG | 1 cut(s) | 654 |
| Eco57I | CTGAAG | 1 cut(s) | 300 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 653 |
| EcoT14I | CCWWGG | 1 cut(s) | 654 |
| ErhI | CCWWGG | 1 cut(s) | 654 |
| FaeI | CATG | 5 cut(s) | 113, 254, 272, 365, 531 |
| FaiI | YATR | 9 cut(s) | 51, 111, 252, 270, 296, 298, 363, 529, 710 |
| FaqI | GGGAC | 2 cut(s) | 31, 342 |
| FatI | CATG | 5 cut(s) | 109, 250, 268, 361, 527 |
| FauNDI | CATATG | 1 cut(s) | 296 |
| FbaI | TGATCA | 1 cut(s) | 118 |
| Fnu4HI | GCNGC | 1 cut(s) | 696 |
| FokI | GGATG | 1 cut(s) | 128 |
| Fsp4HI | GCNGC | 1 cut(s) | 696 |
| GluI | GCNGC | 1 cut(s) | 696 |
| GsaI | CCCAGC | 1 cut(s) | 596 |
| Hin1II | CATG | 5 cut(s) | 113, 254, 272, 365, 531 |
| HincII | GTYRAC | 1 cut(s) | 435 |
| HindII | GTYRAC | 1 cut(s) | 435 |
| HinfI | GANTC | 4 cut(s) | 44, 454, 641, 758 |
| HphI | GGTGA | 2 cut(s) | 611, 745 |
| Hpy166II | GTNNAC | 1 cut(s) | 435 |
| Hpy188I | TCNGA | 1 cut(s) | 205 |
| Hpy188III | TCNNGA | 4 cut(s) | 251, 269, 587, 717 |
| Hpy8I | GTNNAC | 1 cut(s) | 435 |
| Hpy99I | CGWCG | 1 cut(s) | 474 |
| HpyAV | CCTTC | 3 cut(s) | 237, 324, 596 |
| HpyCH4III | ACNGT | 1 cut(s) | 151 |
| HpyCH4IV | ACGT | 4 cut(s) | 431, 472, 613, 754 |
| HpyCH4V | TGCA | 2 cut(s) | 157, 712 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 490 |
| HpyF3I | CTNAG | 7 cut(s) | 8, 99, 189, 452, 522, 548, 704 |
| HpySE526I | ACGT | 4 cut(s) | 431, 472, 613, 754 |
| Hsp92II | CATG | 5 cut(s) | 113, 254, 272, 365, 531 |
| Ksp22I | TGATCA | 1 cut(s) | 118 |
| Kzo9I | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| LpnPI | CCDG | 7 cut(s) | 143, 230, 295, 385, 391, 578, 730 |
| Lsp1109I | GCAGC | 1 cut(s) | 707 |
| MaeII | ACGT | 4 cut(s) | 431, 472, 613, 754 |
| MalI | GATC | 5 cut(s) | 120, 215, 309, 399, 543 |
| MboI | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| MboII | GAAGA | 3 cut(s) | 222, 499, 536 |
| MflI | RGATCY | 1 cut(s) | 541 |
| MhlI | GDGCHC | 1 cut(s) | 100 |
| MluCI | AATT | 6 cut(s) | 69, 91, 206, 259, 634, 742 |
| MlyI | GAGTC | 2 cut(s) | 53, 463 |
| MnlI | CCTC | 5 cut(s) | 19, 68, 106, 532, 594 |
| MseI | TTAA | 3 cut(s) | 239, 263, 555 |
| MslI | CAYNNNNRTG | 1 cut(s) | 114 |
| MvnI | CGCG | 1 cut(s) | 15 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 490 |
| NdeI | CATATG | 1 cut(s) | 296 |
| NdeII | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| NlaIII | CATG | 5 cut(s) | 113, 254, 272, 365, 531 |
| NspV | TTCGAA | 1 cut(s) | 130 |
| PagI | TCATGA | 2 cut(s) | 250, 268 |
| PfeI | GAWTC | 2 cut(s) | 641, 758 |
| PkrI | GCNGC | 1 cut(s) | 697 |
| PleI | GAGTC | 2 cut(s) | 52, 462 |
| PpsI | GAGTC | 2 cut(s) | 52, 462 |
| PspFI | CCCAGC | 1 cut(s) | 592 |
| PsuI | RGATCY | 1 cut(s) | 541 |
| RsaI | GTAC | 1 cut(s) | 532 |
| RsaNI | GTAC | 1 cut(s) | 531 |
| RseI | CAYNNNNRTG | 1 cut(s) | 114 |
| SaqAI | TTAA | 3 cut(s) | 239, 263, 555 |
| SatI | GCNGC | 1 cut(s) | 696 |
| Sau3AI | GATC | 5 cut(s) | 118, 213, 307, 397, 541 |
| SchI | GAGTC | 2 cut(s) | 53, 463 |
| SduI | GDGCHC | 1 cut(s) | 100 |
| SfuI | TTCGAA | 1 cut(s) | 130 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 114 |
| SmlI | CTYRAG | 3 cut(s) | 41, 587, 664 |
| SmoI | CTYRAG | 3 cut(s) | 41, 587, 664 |
| Sse9I | AATT | 6 cut(s) | 69, 91, 206, 259, 634, 742 |
| SsiI | CCGC | 4 cut(s) | 15, 38, 493, 750 |
| StyI | CCWWGG | 1 cut(s) | 654 |
| TaaI | ACNGT | 1 cut(s) | 151 |
| TaiI | ACGT | 4 cut(s) | 434, 475, 616, 757 |
| TaqI | TCGA | 2 cut(s) | 130, 212 |
| TasI | AATT | 6 cut(s) | 69, 91, 206, 259, 634, 742 |
| TatI | WGTACW | 1 cut(s) | 530 |
| TfiI | GAWTC | 2 cut(s) | 641, 758 |
| Tru1I | TTAA | 3 cut(s) | 239, 263, 555 |
| Tru9I | TTAA | 3 cut(s) | 239, 263, 555 |
| TscAI | CASTG | 2 cut(s) | 193, 385 |
| TseI | GCWGC | 1 cut(s) | 695 |
| TspDTI | ATGAA | 3 cut(s) | 180, 283, 285 |
| TspRI | CASTG | 2 cut(s) | 193, 385 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 653 |
| XapI | RAATTY | 1 cut(s) | 206 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 724 |