Major allergen Pru
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 163 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 181, 332 |
| AgsI | TTSAA | 3 cut(s) | 137, 436, 446 |
| AjnI | CCWGG | 1 cut(s) | 466 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 319, 351 |
| AleI | CACNNNNGTG | 1 cut(s) | 177 |
| AluBI | AGCT | 2 cut(s) | 198, 422 |
| AluI | AGCT | 2 cut(s) | 198, 422 |
| Alw21I | GWGCWC | 1 cut(s) | 353 |
| Alw26I | GTCTC | 1 cut(s) | 273 |
| AlwI | GGATC | 1 cut(s) | 163 |
| AoxI | GGCC | 1 cut(s) | 63 |
| ApeKI | GCWGC | 1 cut(s) | 422 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 132 |
| AsuHPI | GGTGA | 4 cut(s) | 22, 152, 191, 386 |
| Bbv12I | GWGCWC | 1 cut(s) | 353 |
| BbvI | GCAGC | 1 cut(s) | 409 |
| BccI | CCATC | 4 cut(s) | 137, 167, 212, 347 |
| BciT130I | CCWGG | 1 cut(s) | 468 |
| BcoDI | GTCTC | 1 cut(s) | 273 |
| BfmI | CTRYAG | 1 cut(s) | 250 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 110 |
| BisI | GCNGC | 1 cut(s) | 423 |
| BlsI | GCNGC | 1 cut(s) | 424 |
| Bme1390I | CCNGG | 1 cut(s) | 468 |
| BmiI | GGNNCC | 2 cut(s) | 157, 337 |
| BmrFI | CCNGG | 1 cut(s) | 468 |
| BmsI | GCATC | 3 cut(s) | 67, 217, 329 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 17, 49, 281, 313 |
| BsaJI | CCNNGG | 2 cut(s) | 175, 466 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 270, 300 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 181, 332 |
| Bse3DI | GCAATG | 1 cut(s) | 364 |
| BseBI | CCWGG | 1 cut(s) | 468 |
| BseDI | CCNNGG | 2 cut(s) | 175, 466 |
| BseGI | GGATG | 2 cut(s) | 39, 90 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 181, 332 |
| BseMI | GCAATG | 1 cut(s) | 364 |
| BseXI | GCAGC | 1 cut(s) | 409 |
| BshFI | GGCC | 1 cut(s) | 65 |
| BsiHKAI | GWGCWC | 1 cut(s) | 353 |
| BslFI | GGGAC | 1 cut(s) | 281 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 181, 332 |
| BsmAI | GTCTC | 1 cut(s) | 273 |
| BsmFI | GGGAC | 1 cut(s) | 281 |
| BsnI | GGCC | 1 cut(s) | 65 |
| Bsp1286I | GDGCHC | 1 cut(s) | 353 |
| Bsp143I | GATC | 3 cut(s) | 168, 384, 438 |
| BspANI | GGCC | 1 cut(s) | 65 |
| BspLI | GGNNCC | 2 cut(s) | 157, 337 |
| BspPI | GGATC | 1 cut(s) | 163 |
| BspQI | GCTCTTC | 1 cut(s) | 387 |
| BsrDI | GCAATG | 1 cut(s) | 364 |
| BssECI | CCNNGG | 2 cut(s) | 175, 466 |
| BssMI | GATC | 3 cut(s) | 168, 384, 438 |
| Bst2UI | CCWGG | 1 cut(s) | 468 |
| Bst6I | CTCTTC | 2 cut(s) | 113, 387 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 179 |
| BstF5I | GGATG | 2 cut(s) | 39, 90 |
| BstKTI | GATC | 3 cut(s) | 171, 387, 441 |
| BstMAI | GTCTC | 1 cut(s) | 273 |
| BstMBI | GATC | 3 cut(s) | 168, 384, 438 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 110, 195 |
| BstNI | CCWGG | 1 cut(s) | 468 |
| BstNSI | RCATGY | 1 cut(s) | 401 |
| BstSCI | CCNGG | 1 cut(s) | 466 |
| BstSFI | CTRYAG | 1 cut(s) | 250 |
| BstV1I | GCAGC | 1 cut(s) | 409 |
| BstX2I | RGATCY | 1 cut(s) | 168 |
| BstYI | RGATCY | 1 cut(s) | 168 |
| BsuRI | GGCC | 1 cut(s) | 65 |
| BtsCI | GGATG | 2 cut(s) | 39, 90 |
| CseI | GACGC | 1 cut(s) | 278 |
| CviAII | CATG | 1 cut(s) | 398 |
| CviJI | RGCY | 5 cut(s) | 65, 113, 198, 407, 422 |
| CviKI_1 | RGCY | 5 cut(s) | 65, 113, 198, 407, 422 |
| DpnI | GATC | 3 cut(s) | 170, 386, 440 |
| DpnII | GATC | 3 cut(s) | 168, 384, 438 |
| Eam1104I | CTCTTC | 2 cut(s) | 113, 387 |
| EarI | CTCTTC | 2 cut(s) | 113, 387 |
| Eco147I | AGGCCT | 1 cut(s) | 65 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 179 |
| EcoRII | CCWGG | 1 cut(s) | 466 |
| FaeI | CATG | 1 cut(s) | 401 |
| FaiI | YATR | 4 cut(s) | 18, 201, 252, 399 |
| FaqI | GGGAC | 1 cut(s) | 281 |
| FatI | CATG | 1 cut(s) | 397 |
| Fnu4HI | GCNGC | 1 cut(s) | 423 |
| FokI | GGATG | 2 cut(s) | 26, 77 |
| Fsp4HI | GCNGC | 1 cut(s) | 423 |
| GluI | GCNGC | 1 cut(s) | 423 |
| HaeIII | GGCC | 1 cut(s) | 65 |
| HgaI | GACGC | 1 cut(s) | 278 |
| Hin1II | CATG | 1 cut(s) | 401 |
| HinfI | GANTC | 1 cut(s) | 54 |
| HphI | GGTGA | 4 cut(s) | 22, 152, 191, 386 |
| Hpy166II | GTNNAC | 3 cut(s) | 12, 30, 244 |
| Hpy188I | TCNGA | 2 cut(s) | 213, 278 |
| Hpy188III | TCNNGA | 3 cut(s) | 163, 346, 436 |
| Hpy8I | GTNNAC | 3 cut(s) | 12, 30, 244 |
| HpyCH4V | TGCA | 1 cut(s) | 369 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 110, 195 |
| Hsp92II | CATG | 1 cut(s) | 401 |
| Kzo9I | GATC | 3 cut(s) | 168, 384, 438 |
| LguI | GCTCTTC | 1 cut(s) | 387 |
| LpnPI | CCDG | 5 cut(s) | 95, 367, 393, 411, 453 |
| Lsp1109I | GCAGC | 1 cut(s) | 409 |
| LweI | GCATC | 3 cut(s) | 67, 217, 329 |
| MalI | GATC | 3 cut(s) | 170, 386, 440 |
| MboI | GATC | 3 cut(s) | 168, 384, 438 |
| MboII | GAAGA | 3 cut(s) | 130, 178, 404 |
| MflI | RGATCY | 1 cut(s) | 168 |
| MhlI | GDGCHC | 1 cut(s) | 353 |
| MluCI | AATT | 1 cut(s) | 311 |
| MlyI | GAGTC | 1 cut(s) | 48 |
| MmeI | TCCRAC | 1 cut(s) | 133 |
| MnlI | CCTC | 8 cut(s) | 43, 98, 140, 175, 185, 256, 326, 370 |
| MroXI | GAANNNNTTC | 1 cut(s) | 132 |
| MseI | TTAA | 1 cut(s) | 309 |
| MslI | CAYNNNNRTG | 1 cut(s) | 177 |
| MspR9I | CCNGG | 1 cut(s) | 468 |
| MvaI | CCWGG | 1 cut(s) | 468 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 110, 195 |
| NdeII | GATC | 3 cut(s) | 168, 384, 438 |
| NlaIII | CATG | 1 cut(s) | 401 |
| NlaIV | GGNNCC | 2 cut(s) | 157, 337 |
| NspI | RCATGY | 1 cut(s) | 401 |
| OliI | CACNNNNGTG | 1 cut(s) | 177 |
| PceI | AGGCCT | 1 cut(s) | 65 |
| PciSI | GCTCTTC | 1 cut(s) | 387 |
| PdmI | GAANNNNTTC | 1 cut(s) | 132 |
| PkrI | GCNGC | 1 cut(s) | 424 |
| PleI | GAGTC | 1 cut(s) | 48 |
| PpsI | GAGTC | 1 cut(s) | 48 |
| Psp6I | CCWGG | 1 cut(s) | 466 |
| PspGI | CCWGG | 1 cut(s) | 466 |
| PspN4I | GGNNCC | 2 cut(s) | 157, 337 |
| PsuI | RGATCY | 1 cut(s) | 168 |
| RseI | CAYNNNNRTG | 1 cut(s) | 177 |
| SapI | GCTCTTC | 1 cut(s) | 387 |
| SaqAI | TTAA | 1 cut(s) | 309 |
| SatI | GCNGC | 1 cut(s) | 423 |
| Sau3AI | GATC | 3 cut(s) | 168, 384, 438 |
| SchI | GAGTC | 1 cut(s) | 48 |
| ScrFI | CCNGG | 1 cut(s) | 468 |
| SduI | GDGCHC | 1 cut(s) | 353 |
| SfaNI | GCATC | 3 cut(s) | 67, 217, 329 |
| SfcI | CTRYAG | 1 cut(s) | 250 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 177 |
| Sse9I | AATT | 1 cut(s) | 311 |
| SseBI | AGGCCT | 1 cut(s) | 65 |
| StuI | AGGCCT | 1 cut(s) | 65 |
| StyD4I | CCNGG | 1 cut(s) | 466 |
| TasI | AATT | 1 cut(s) | 311 |
| Tru1I | TTAA | 1 cut(s) | 309 |
| Tru9I | TTAA | 1 cut(s) | 309 |
| TseI | GCWGC | 1 cut(s) | 422 |
| TspDTI | ATGAA | 1 cut(s) | 33 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 179 |
| XceI | RCATGY | 1 cut(s) | 401 |
| XmnI | GAANNNNTTC | 1 cut(s) | 132 |