Major allergen Pru
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 109 |
| AccB1I | GGYRCC | 1 cut(s) | 109 |
| AccI | GTMKAC | 1 cut(s) | 26 |
| AfaI | GTAC | 1 cut(s) | 111 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 144, 210 |
| AgsI | TTSAA | 1 cut(s) | 210 |
| AjiI | CACGTC | 1 cut(s) | 174 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 214, 246 |
| AluBI | AGCT | 2 cut(s) | 32, 52 |
| AluI | AGCT | 2 cut(s) | 32, 52 |
| Alw21I | GWGCWC | 4 cut(s) | 54, 127, 173, 239 |
| AoxI | GGCC | 1 cut(s) | 179 |
| Asp718I | GGTACC | 1 cut(s) | 109 |
| AsuC2I | CCSGG | 1 cut(s) | 183 |
| AsuHPI | GGTGA | 2 cut(s) | 160, 193 |
| BanI | GGYRCC | 1 cut(s) | 109 |
| BanII | GRGCYC | 1 cut(s) | 54 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 61, 93 |
| Bbv12I | GWGCWC | 4 cut(s) | 54, 127, 173, 239 |
| BcnI | CCSGG | 1 cut(s) | 183 |
| BfmI | CTRYAG | 2 cut(s) | 27, 33 |
| BglII | AGATCT | 1 cut(s) | 71 |
| Bme1390I | CCNGG | 1 cut(s) | 183 |
| BmgBI | CACGTC | 1 cut(s) | 174 |
| BmiI | GGNNCC | 1 cut(s) | 111 |
| BmrFI | CCNGG | 1 cut(s) | 183 |
| BmsI | GCATC | 2 cut(s) | 106, 234 |
| BpuMI | CCSGG | 1 cut(s) | 183 |
| BsaAI | YACGTR | 1 cut(s) | 81 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 144, 210 |
| Bse1I | ACTGG | 1 cut(s) | 128 |
| BseGI | GGATG | 1 cut(s) | 97 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 144, 210 |
| BseNI | ACTGG | 1 cut(s) | 128 |
| BshFI | GGCC | 1 cut(s) | 181 |
| BshNI | GGYRCC | 1 cut(s) | 109 |
| BsiHKAI | GWGCWC | 4 cut(s) | 54, 127, 173, 239 |
| BsiSI | CCGG | 2 cut(s) | 101, 182 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 144, 210 |
| BsnI | GGCC | 1 cut(s) | 181 |
| Bsp1286I | GDGCHC | 4 cut(s) | 54, 127, 173, 239 |
| Bsp143I | GATC | 1 cut(s) | 71 |
| BspANI | GGCC | 1 cut(s) | 181 |
| BspLI | GGNNCC | 1 cut(s) | 111 |
| BspQI | GCTCTTC | 1 cut(s) | 161 |
| BspT107I | GGYRCC | 1 cut(s) | 109 |
| BsrI | ACTGG | 1 cut(s) | 128 |
| BssMI | GATC | 1 cut(s) | 71 |
| Bst4CI | ACNGT | 2 cut(s) | 37, 114 |
| Bst6I | CTCTTC | 2 cut(s) | 161, 211 |
| BstBAI | YACGTR | 1 cut(s) | 81 |
| BstDEI | CTNAG | 1 cut(s) | 86 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 208 |
| BstF5I | GGATG | 1 cut(s) | 97 |
| BstKTI | GATC | 1 cut(s) | 74 |
| BstMBI | GATC | 1 cut(s) | 71 |
| BstSCI | CCNGG | 1 cut(s) | 181 |
| BstSFI | CTRYAG | 2 cut(s) | 27, 33 |
| BstX2I | RGATCY | 1 cut(s) | 71 |
| BstYI | RGATCY | 1 cut(s) | 71 |
| BsuRI | GGCC | 1 cut(s) | 181 |
| BtrI | CACGTC | 1 cut(s) | 174 |
| BtsCI | GGATG | 1 cut(s) | 97 |
| BtsI | GCAGTG | 1 cut(s) | 111 |
| BtsIMutI | CAGTG | 3 cut(s) | 42, 111, 135 |
| Csp6I | GTAC | 1 cut(s) | 110 |
| CviAII | CATG | 1 cut(s) | 139 |
| CviJI | RGCY | 4 cut(s) | 32, 52, 181, 196 |
| CviKI_1 | RGCY | 4 cut(s) | 32, 52, 181, 196 |
| CviQI | GTAC | 1 cut(s) | 110 |
| DdeI | CTNAG | 1 cut(s) | 86 |
| DpnI | GATC | 1 cut(s) | 73 |
| DpnII | GATC | 1 cut(s) | 71 |
| Eam1104I | CTCTTC | 2 cut(s) | 161, 211 |
| EarI | CTCTTC | 2 cut(s) | 161, 211 |
| Ecl136II | GAGCTC | 1 cut(s) | 52 |
| Eco24I | GRGCYC | 1 cut(s) | 54 |
| Eco32I | GATATC | 1 cut(s) | 159 |
| Eco53kI | GAGCTC | 1 cut(s) | 52 |
| EcoICRI | GAGCTC | 1 cut(s) | 52 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 208 |
| EcoRV | GATATC | 1 cut(s) | 159 |
| EcoT38I | GRGCYC | 1 cut(s) | 54 |
| FaeI | CATG | 1 cut(s) | 142 |
| FaiI | YATR | 1 cut(s) | 140 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 36 |
| FatI | CATG | 1 cut(s) | 138 |
| FblI | GTMKAC | 1 cut(s) | 26 |
| FokI | GGATG | 1 cut(s) | 84 |
| FriOI | GRGCYC | 1 cut(s) | 54 |
| HaeIII | GGCC | 1 cut(s) | 181 |
| HapII | CCGG | 2 cut(s) | 101, 182 |
| Hin1II | CATG | 1 cut(s) | 142 |
| HinfI | GANTC | 1 cut(s) | 58 |
| HpaII | CCGG | 2 cut(s) | 101, 182 |
| HphI | GGTGA | 2 cut(s) | 160, 193 |
| Hpy166II | GTNNAC | 1 cut(s) | 27 |
| Hpy188III | TCNNGA | 3 cut(s) | 55, 66, 120 |
| Hpy8I | GTNNAC | 1 cut(s) | 27 |
| HpyAV | CCTTC | 1 cut(s) | 186 |
| HpyCH4III | ACNGT | 2 cut(s) | 37, 114 |
| HpyCH4IV | ACGT | 2 cut(s) | 80, 173 |
| HpyF3I | CTNAG | 1 cut(s) | 86 |
| HpySE526I | ACGT | 2 cut(s) | 80, 173 |
| Hsp92II | CATG | 1 cut(s) | 142 |
| KpnI | GGTACC | 1 cut(s) | 113 |
| Kzo9I | GATC | 1 cut(s) | 71 |
| LguI | GCTCTTC | 1 cut(s) | 161 |
| LmnI | GCTCC | 3 cut(s) | 49, 57, 234 |
| LpnPI | CCDG | 4 cut(s) | 68, 114, 141, 195 |
| LweI | GCATC | 2 cut(s) | 106, 234 |
| MaeII | ACGT | 2 cut(s) | 80, 173 |
| MaeIII | GTNAC | 1 cut(s) | 81 |
| MalI | GATC | 1 cut(s) | 73 |
| MboI | GATC | 1 cut(s) | 71 |
| MboII | GAAGA | 3 cut(s) | 81, 178, 198 |
| MflI | RGATCY | 1 cut(s) | 71 |
| MhlI | GDGCHC | 4 cut(s) | 54, 127, 173, 239 |
| MlyI | GAGTC | 1 cut(s) | 52 |
| MnlI | CCTC | 3 cut(s) | 39, 214, 238 |
| MslI | CAYNNNNRTG | 1 cut(s) | 242 |
| MspI | CCGG | 2 cut(s) | 101, 182 |
| MspR9I | CCNGG | 1 cut(s) | 183 |
| NciI | CCSGG | 1 cut(s) | 183 |
| NdeII | GATC | 1 cut(s) | 71 |
| NlaIII | CATG | 1 cut(s) | 142 |
| NlaIV | GGNNCC | 1 cut(s) | 111 |
| NmuCI | GTSAC | 1 cut(s) | 81 |
| PciSI | GCTCTTC | 1 cut(s) | 161 |
| PleI | GAGTC | 1 cut(s) | 52 |
| PpsI | GAGTC | 1 cut(s) | 52 |
| Ppu21I | YACGTR | 1 cut(s) | 81 |
| Psp124BI | GAGCTC | 1 cut(s) | 54 |
| PspN4I | GGNNCC | 1 cut(s) | 111 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 10, 42 |
| PsuI | RGATCY | 1 cut(s) | 71 |
| RsaI | GTAC | 1 cut(s) | 111 |
| RsaNI | GTAC | 1 cut(s) | 110 |
| RseI | CAYNNNNRTG | 1 cut(s) | 242 |
| SacI | GAGCTC | 1 cut(s) | 54 |
| SapI | GCTCTTC | 1 cut(s) | 161 |
| Sau3AI | GATC | 1 cut(s) | 71 |
| SchI | GAGTC | 1 cut(s) | 52 |
| ScrFI | CCNGG | 1 cut(s) | 183 |
| SduI | GDGCHC | 4 cut(s) | 54, 127, 173, 239 |
| SetI | ASST | 7 cut(s) | 34, 54, 83, 176, 206, 215, 230 |
| SfaNI | GCATC | 2 cut(s) | 106, 234 |
| SfcI | CTRYAG | 2 cut(s) | 27, 33 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 242 |
| SstI | GAGCTC | 1 cut(s) | 54 |
| StyD4I | CCNGG | 1 cut(s) | 181 |
| TaaI | ACNGT | 2 cut(s) | 37, 114 |
| TaiI | ACGT | 2 cut(s) | 83, 176 |
| TaqI | TCGA | 1 cut(s) | 65 |
| TscAI | CASTG | 3 cut(s) | 42, 111, 135 |
| TseFI | GTSAC | 1 cut(s) | 81 |
| Tsp45I | GTSAC | 1 cut(s) | 81 |
| TspRI | CASTG | 3 cut(s) | 42, 111, 135 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 208 |
| XmiI | GTMKAC | 1 cut(s) | 26 |