RchiOBHm_Chr7g0230371

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001555
Physical Location & Seq
Forward (+)
53878517 .. 53881193
2677 bp
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UTR
Exon/CDS
Intron
PRQ20641

Sequence Viewer

Length: 588 bp
ATGTCTGCTCAAGCTCCCACCATTCCTCTCCTCACTCCTTACAAACTGGGAAAGTTCAATCTCTCTCATCGAGTTGTTTTGGCACCCTTGACTAGATGGAGATCATACAACAATCTTCCTCAACCGCATGCCATCTTATATTACTCTCAGCGAACAACCAATGGGGGTCTTTTAATAGCTGAAGCCAGTGGAGTTTCTGACACAGCTCAAGGGTTTGCGGATATGCCTGGTTTATGGTCAAAGGAACAAGTTGAAGCATGGAAACCCATAGTTGATGCAGTTCATGCTAAAGGTGGTGTATTCTTCTGTCAGATCTGGCATGCGGGAAGAGTTTCAAATAGTGACAAAGTCTCTTGTGTTCAGATTGTGATTCTTATCTCTTATACTTTCAGTTTTTCAAACAAATGGTCAGCTCCAATTTCTTGTACTGACAAGCCACTAACCCCTCAAGTACTATCTGATGGTGTTGACGTTGCTGAATTCACTCCTCCAAGGCAATTAAGGACAGATGAAATCCCACAAATTGTCAATGACTTTAGACTTGCTGCACTGAATGCAATCGAAGCTGATAATTTTCCTTCAACTTGA

Protein Analysis

195

Amino Acids

21.49

Weight (kDa)

6.89

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 10 - 189 1.2e-29 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 82
AciI CCGC 3 cut(s) 125, 218, 323
AcsI RAATTY 1 cut(s) 479
AcuI CTGAAG 1 cut(s) 201
AfaI GTAC 2 cut(s) 427, 453
AgsI TTSAA 5 cut(s) 58, 254, 336, 399, 582
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 5 cut(s) 14, 179, 206, 413, 566
AluI AGCT 5 cut(s) 14, 179, 206, 413, 566
Alw26I GTCTC 1 cut(s) 355
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 479
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
Asp700I GAANNNNTTC 1 cut(s) 331
BanI GGYRCC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 532
BccI CCATC 3 cut(s) 90, 140, 455
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 355
BfaI CTAG 1 cut(s) 93
BglII AGATCT 1 cut(s) 312
BisI GCNGC 1 cut(s) 546
BlsI GCNGC 1 cut(s) 547
BmcAI AGTACT 1 cut(s) 453
Bme1390I CCNGG 1 cut(s) 228
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 228
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 1 cut(s) 265
BmuI ACTGGG 1 cut(s) 56
BpuEI CTTGAG 2 cut(s) 192, 432
BsaBI GATNNNNATC 2 cut(s) 100, 374
BsaJI CCNNGG 1 cut(s) 491
Bse1I ACTGG 2 cut(s) 51, 186
Bse8I GATNNNNATC 2 cut(s) 100, 374
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 491
BseJI GATNNNNATC 2 cut(s) 100, 374
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 2 cut(s) 51, 186
BseRI GAGGAG 2 cut(s) 20, 477
BseXI GCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 531
BshNI GGYRCC 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 355
BsmI GAATGC 1 cut(s) 559
Bsp143I GATC 2 cut(s) 101, 312
BspACI CCGC 3 cut(s) 125, 218, 323
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 82
BsrI ACTGG 2 cut(s) 51, 186
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 2 cut(s) 101, 312
BssT1I CCWWGG 1 cut(s) 491
Bst2UI CCWGG 1 cut(s) 228
Bst6I CTCTTC 1 cut(s) 322
BstAPI GCANNNNNTGC 2 cut(s) 284, 554
BstC8I GCNNGC 2 cut(s) 129, 321
BstDEI CTNAG 1 cut(s) 147
BstKTI GATC 2 cut(s) 104, 315
BstMAI GTCTC 1 cut(s) 355
BstMBI GATC 2 cut(s) 101, 312
BstMWI GCNNNNNNNGC 3 cut(s) 284, 554, 563
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 2 cut(s) 131, 323
BstSCI CCNGG 1 cut(s) 226
BstV1I GCAGC 1 cut(s) 532
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 193, 548
Cac8I GCNNGC 2 cut(s) 129, 321
Csp6I GTAC 2 cut(s) 426, 452
CviAII CATG 4 cut(s) 128, 258, 284, 320
CviJI RGCY 7 cut(s) 14, 179, 185, 206, 413, 436, 566
CviKI_1 RGCY 7 cut(s) 14, 179, 185, 206, 413, 436, 566
CviQI GTAC 2 cut(s) 426, 452
DdeI CTNAG 1 cut(s) 147
DpnI GATC 2 cut(s) 103, 314
DpnII GATC 2 cut(s) 101, 312
Eam1104I CTCTTC 1 cut(s) 322
EarI CTCTTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 479
EcoRII CCWGG 1 cut(s) 226
EcoT14I CCWWGG 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 491
FaeI CATG 4 cut(s) 131, 261, 287, 323
FatI CATG 4 cut(s) 127, 257, 283, 319
FauI CCCGC 1 cut(s) 316
Fnu4HI GCNGC 1 cut(s) 546
Fsp4HI GCNGC 1 cut(s) 546
FspBI CTAG 1 cut(s) 93
GluI GCNGC 1 cut(s) 546
Hin1II CATG 4 cut(s) 131, 261, 287, 323
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HinfI GANTC 1 cut(s) 370
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 4 cut(s) 199, 312, 363, 460
Hpy8I GTNNAC 1 cut(s) 469
HpyAV CCTTC 1 cut(s) 588
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 3 cut(s) 278, 548, 557
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 554, 563
HpyF3I CTNAG 1 cut(s) 147
HpySE526I ACGT 1 cut(s) 471
Hsp92II CATG 4 cut(s) 131, 261, 287, 323
Kzo9I GATC 2 cut(s) 101, 312
LmnI GCTCC 2 cut(s) 19, 418
LpnPI CCDG 5 cut(s) 32, 199, 213, 240, 301
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 1 cut(s) 265
MaeI CTAG 1 cut(s) 93
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 1 cut(s) 341
MalI GATC 2 cut(s) 103, 314
MboI GATC 2 cut(s) 101, 312
MboII GAAGA 3 cut(s) 107, 295, 339
MflI RGATCY 1 cut(s) 312
MluCI AATT 5 cut(s) 417, 479, 497, 522, 571
MnlI CCTC 5 cut(s) 36, 41, 129, 456, 498
MroXI GAANNNNTTC 1 cut(s) 331
MseI TTAA 2 cut(s) 173, 500
MspR9I CCNGG 1 cut(s) 228
Mva1269I GAATGC 1 cut(s) 559
MvaI CCWGG 1 cut(s) 228
MwoI GCNNNNNNNGC 3 cut(s) 284, 554, 563
NdeII GATC 2 cut(s) 101, 312
NlaIII CATG 4 cut(s) 131, 261, 287, 323
NlaIV GGNNCC 1 cut(s) 84
NmuCI GTSAC 1 cut(s) 341
NspI RCATGY 2 cut(s) 131, 323
PaeI GCATGC 2 cut(s) 131, 323
PctI GAATGC 1 cut(s) 559
PdmI GAANNNNTTC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 370
PflFI GACNNNGTC 1 cut(s) 347
PkrI GCNGC 1 cut(s) 547
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 84
PsuI RGATCY 1 cut(s) 312
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 2 cut(s) 427, 453
RsaNI GTAC 2 cut(s) 426, 452
SaqAI TTAA 2 cut(s) 173, 500
SatI GCNGC 1 cut(s) 546
Sau3AI GATC 2 cut(s) 101, 312
ScaI AGTACT 1 cut(s) 453
ScrFI CCNGG 1 cut(s) 228
SetI ASST 7 cut(s) 16, 181, 208, 295, 415, 474, 568
SfaNI GCATC 1 cut(s) 265
SmlI CTYRAG 3 cut(s) 9, 207, 447
SmoI CTYRAG 3 cut(s) 9, 207, 447
SphI GCATGC 2 cut(s) 131, 323
Sse9I AATT 5 cut(s) 417, 479, 497, 522, 571
SsiI CCGC 3 cut(s) 125, 218, 323
SspMI CTAG 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 491
TaiI ACGT 1 cut(s) 474
TaqI TCGA 2 cut(s) 70, 561
TasI AATT 5 cut(s) 417, 479, 497, 522, 571
TatI WGTACW 2 cut(s) 425, 451
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 2 cut(s) 173, 500
Tru9I TTAA 2 cut(s) 173, 500
TscAI CASTG 2 cut(s) 193, 555
TseFI GTSAC 1 cut(s) 341
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 341
TspDTI ATGAA 2 cut(s) 272, 525
TspRI CASTG 2 cut(s) 193, 555
Tth111I GACNNNGTC 1 cut(s) 347
XapI RAATTY 1 cut(s) 479
XceI RCATGY 2 cut(s) 131, 323
XmnI GAANNNNTTC 1 cut(s) 331
XspI CTAG 1 cut(s) 93
ZrmI AGTACT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.