RchiOBHm_Chr7g0230421

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001555
Physical Location & Seq
Forward (+)
53915260 .. 53915505
246 bp
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UTR
Exon/CDS
Intron
PRQ20646

Sequence Viewer

Length: 246 bp
ATGAGAGAAGCTTTTAGTGGTACCTTCATGGCTAATGGTGGTTTTGATAGGGAAGATGGGAACAATGCAGTGGCTGAGGGTGGTGCGGATCTTATTGCTTACGGCCGTTGGTTCTTGTCTAATCCTGATTTGCCAAAGAGATTTGAGTTTAATGCTCCTCTAAACAAGTATGACAAAAGTATTTTCTGTACTTCTGCTCCAGTTGTTGGTTACACTGATTATCCATTTCTTGAGACCACTGCTTAA

Protein Analysis

81

Amino Acids

8.95

Weight (kDa)

4.5

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 2 - 54 1.1e-07 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 20
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 206
AciI CCGC 1 cut(s) 86
AclWI GGATC 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 103
AfaI GTAC 2 cut(s) 22, 190
AfiI CCNNNNNNNGG 1 cut(s) 206
AluBI AGCT 1 cut(s) 11
AluI AGCT 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 96
AlwNI CAGNNNCTG 1 cut(s) 74
AoxI GGCC 1 cut(s) 103
Asp718I GGTACC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 20
BbvCI CCTCAGC 1 cut(s) 75
BccI CCATC 1 cut(s) 50
BceAI ACGGC 2 cut(s) 90, 118
BcoDI GTCTC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 22
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 200
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 147
BseX3I CGGCCG 1 cut(s) 103
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 1 cut(s) 105
BshNI GGYRCC 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 227
BsnI GGCC 1 cut(s) 105
Bso31I GGTCTC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 88
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 105
BspCNI CTCAG 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 22
BspPI GGATC 1 cut(s) 96
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 227
BsrI ACTGG 1 cut(s) 200
BssMI GATC 1 cut(s) 88
BstDEI CTNAG 1 cut(s) 75
BstKTI GATC 1 cut(s) 91
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 1 cut(s) 88
BstMCI CGRYCG 1 cut(s) 106
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 1 cut(s) 105
BtsI GCAGTG 2 cut(s) 75, 237
BtsIMutI CAGTG 3 cut(s) 75, 213, 237
CaiI CAGNNNCTG 1 cut(s) 74
Csp6I GTAC 2 cut(s) 21, 189
CviAII CATG 1 cut(s) 28
CviJI RGCY 4 cut(s) 11, 32, 74, 105
CviKI_1 RGCY 4 cut(s) 11, 32, 74, 105
CviQI GTAC 2 cut(s) 21, 189
DdeI CTNAG 1 cut(s) 75
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 103
Eco31I GGTCTC 1 cut(s) 227
Eco52I CGGCCG 1 cut(s) 103
FaeI CATG 1 cut(s) 31
FaiI YATR 2 cut(s) 29, 171
FatI CATG 1 cut(s) 27
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 1 cut(s) 105
Hin1II CATG 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 9
Hpy188III TCNNGA 2 cut(s) 125, 230
HpyAV CCTTC 1 cut(s) 34
HpyCH4V TGCA 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 75
Hsp92II CATG 1 cut(s) 31
KpnI GGTACC 1 cut(s) 24
Kzo9I GATC 1 cut(s) 88
LmnI GCTCC 2 cut(s) 160, 202
LpnPI CCDG 2 cut(s) 138, 213
MaeIII GTNAC 1 cut(s) 209
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 1 cut(s) 65
MflI RGATCY 1 cut(s) 88
MnlI CCTC 2 cut(s) 70, 168
MseI TTAA 2 cut(s) 150, 244
NdeII GATC 1 cut(s) 88
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 1 cut(s) 22
PflMI CCANNNNNTGG 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 88
RsaI GTAC 2 cut(s) 22, 190
RsaNI GTAC 2 cut(s) 21, 189
SaqAI TTAA 2 cut(s) 150, 244
Sau3AI GATC 1 cut(s) 88
SetI ASST 2 cut(s) 13, 26
SgeI CNNG 6 cut(s) 40, 127, 137, 178, 212, 242
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SsiI CCGC 1 cut(s) 86
TatI WGTACW 1 cut(s) 188
Tru1I TTAA 2 cut(s) 150, 244
Tru9I TTAA 2 cut(s) 150, 244
TscAI CASTG 3 cut(s) 75, 220, 244
TspDTI ATGAA 1 cut(s) 16
TspRI CASTG 3 cut(s) 75, 220, 244
Van91I CCANNNNNTGG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.