RchiOBHm_Chr7g0230411

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001555
Physical Location & Seq
Forward (+)
53914649 .. 53915155
507 bp
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UTR
Exon/CDS
Intron
PRQ20645

Sequence Viewer

Length: 252 bp
ATGAACCACTATACATCTCGCTTTGATGGGGTTGAAATTCATGGACCTAATGGTTACCTTATTAATCAGTTTCTGAGAGACCAAGTGAATGATCGAACAGACCAATATGGTGGATCTCTAGAGAATTGCTGCCGATTTGCTCTGATTGAAGCTGTTATTGAAATGGGAGCAGATAAAGTTGGAATTAGATTATCTCCATTCGTGGACTATATGGAATCAGGGGATTCAAACCCTGAAGCATTAGGCTTTTAA

Protein Analysis

83

Amino Acids

9.31

Weight (kDa)

4.46

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 7 - 75 7.6e-24 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 121
AcsI RAATTY 1 cut(s) 36
AgsI TTSAA 4 cut(s) 35, 149, 161, 228
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 1 cut(s) 121
AlwNI CAGNNNCTG 1 cut(s) 73
ApeKI GCWGC 1 cut(s) 129
ApoI RAATTY 1 cut(s) 36
AseI ATTAAT 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 44
AvaII GGWCC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 116
BccI CCATC 1 cut(s) 20
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 1 cut(s) 119
BisI GCNGC 1 cut(s) 130
BlsI GCNGC 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 72
BseMII CTCAG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 72
Bso31I GGTCTC 1 cut(s) 72
Bsp143I GATC 2 cut(s) 91, 113
BspCNI CTCAG 1 cut(s) 66
BspPI GGATC 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 72
BssMI GATC 2 cut(s) 91, 113
BstDEI CTNAG 1 cut(s) 74
BstEII GGTNACC 1 cut(s) 53
BstKTI GATC 2 cut(s) 94, 116
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 2 cut(s) 91, 113
BstPI GGTNACC 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 113
BstXI CCANNNNNNTGG 1 cut(s) 110
BstYI RGATCY 1 cut(s) 113
CaiI CAGNNNCTG 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 44
CviAII CATG 1 cut(s) 41
CviJI RGCY 2 cut(s) 152, 246
CviKI_1 RGCY 2 cut(s) 152, 246
DdeI CTNAG 1 cut(s) 74
DpnI GATC 2 cut(s) 93, 115
DpnII GATC 2 cut(s) 91, 113
Eco31I GGTCTC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 53
EcoO65I GGTNACC 1 cut(s) 53
FaeI CATG 1 cut(s) 44
FaiI YATR 5 cut(s) 12, 42, 108, 210, 212
FatI CATG 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 130
Fsp4HI GCNGC 1 cut(s) 130
FspBI CTAG 1 cut(s) 119
GluI GCNGC 1 cut(s) 130
Hin1II CATG 1 cut(s) 44
HinfI GANTC 2 cut(s) 215, 224
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 75, 144
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 1 cut(s) 205
HpyF3I CTNAG 1 cut(s) 74
Hsp92II CATG 1 cut(s) 44
Kzo9I GATC 2 cut(s) 91, 113
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 2 cut(s) 204, 246
Lsp1109I GCAGC 1 cut(s) 116
MaeI CTAG 1 cut(s) 119
MaeIII GTNAC 1 cut(s) 53
MalI GATC 2 cut(s) 93, 115
MboI GATC 2 cut(s) 91, 113
MflI RGATCY 1 cut(s) 113
MluCI AATT 3 cut(s) 36, 124, 183
MmeI TCCRAC 1 cut(s) 160
MseI TTAA 2 cut(s) 63, 250
NdeII GATC 2 cut(s) 91, 113
NlaIII CATG 1 cut(s) 44
PfeI GAWTC 2 cut(s) 215, 224
PkrI GCNGC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 63
PspEI GGTNACC 1 cut(s) 53
PspPI GGNCC 1 cut(s) 44
PstNI CAGNNNCTG 1 cut(s) 73
PsuI RGATCY 1 cut(s) 113
SaqAI TTAA 2 cut(s) 63, 250
SatI GCNGC 1 cut(s) 130
Sau3AI GATC 2 cut(s) 91, 113
Sau96I GGNCC 1 cut(s) 44
SetI ASST 3 cut(s) 49, 60, 154
SgeI CNNG 7 cut(s) 30, 53, 95, 131, 214, 231, 245
SinI GGWCC 1 cut(s) 44
Sse9I AATT 3 cut(s) 36, 124, 183
SspMI CTAG 1 cut(s) 119
TaqI TCGA 1 cut(s) 94
TasI AATT 3 cut(s) 36, 124, 183
TfiI GAWTC 2 cut(s) 215, 224
Tru1I TTAA 2 cut(s) 63, 250
Tru9I TTAA 2 cut(s) 63, 250
TseI GCWGC 1 cut(s) 129
TspDTI ATGAA 2 cut(s) 17, 29
VpaK11BI GGWCC 1 cut(s) 44
VspI ATTAAT 1 cut(s) 63
XapI RAATTY 1 cut(s) 36
XbaI TCTAGA 1 cut(s) 118
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.