RchiOBHm_Chr7g0230381

12-oxophytodienoate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001555
Physical Location & Seq
Forward (+)
53890387 .. 53891273
887 bp
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UTR
Exon/CDS
Intron
PRQ20642

Sequence Viewer

Length: 444 bp
ATGAAAGACCAAGTTAATGATCGAACAGACCAATATGGTGGATCTCTAGAGAATCGCTGCCGATTTGCTCTGGAAATCATTGAAGCAGTTGTTAACAAACAGGAGCAGATAAAGTTGGAATTAGATTATCTCCATTTGCCGACTATATGGAATCAGGGGATTCAAACCCTTGAAGCATTGGGCCTTTATATGGTTCAATCCTTGAACAAATATGGGATTCTGTACTGCCATGTGATTGAACCAAGAATGAAGGAAGCTGCAGTGAAAAGTGAAAGTGCTGACAGTCTTCTACCCACGAGAAAGGCTTTTAATGGTACCTTCATTGCTGCTAGTAGTTTTGACAGGGAAGATGGGAACAATGCAGTGGCTGAGGGCTGTGCAGATCTTATTGCTTACGACCGTTGGTTCTTGTCTAATCCTGATTTGCCAAAGAAACAAGTATGA

Protein Analysis

147

Amino Acids

16.64

Weight (kDa)

5.04

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_FMN PF00724 3 - 145 4.1e-23 NADH:flavin oxidoreductase / NADH oxidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 314
AclWI GGATC 1 cut(s) 49
AfaI GTAC 2 cut(s) 224, 316
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 6 cut(s) 83, 164, 173, 197, 205, 239
AluBI AGCT 1 cut(s) 257
AluI AGCT 1 cut(s) 257
AlwI GGATC 1 cut(s) 49
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 3 cut(s) 57, 257, 326
Asp718I GGTACC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 181
BanI GGYRCC 1 cut(s) 314
BauI CACGAG 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 278
BbvCI CCTCAGC 1 cut(s) 369
BbvI GCAGC 3 cut(s) 44, 244, 313
BccI CCATC 1 cut(s) 344
BfaI CTAG 2 cut(s) 47, 330
BfmI CTRYAG 1 cut(s) 258
BglII AGATCT 1 cut(s) 382
BisI GCNGC 3 cut(s) 58, 258, 327
BlsI GCNGC 3 cut(s) 59, 259, 328
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 1 cut(s) 316
BpiI GAAGAC 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 369
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 1 cut(s) 360
BseXI GCAGC 3 cut(s) 44, 244, 313
BsgI GTGCAG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 400
BshFI GGCC 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 400
BslI CCNNNNNNNGG 1 cut(s) 190
BsnI GGCC 1 cut(s) 183
Bsp143I GATC 3 cut(s) 19, 41, 382
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 316
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 321
BssMI GATC 3 cut(s) 19, 41, 382
BssSI CACGAG 1 cut(s) 295
Bst2BI CACGAG 1 cut(s) 295
Bst4CI ACNGT 2 cut(s) 284, 401
BstDEI CTNAG 1 cut(s) 369
BstKTI GATC 3 cut(s) 22, 44, 385
BstMBI GATC 3 cut(s) 19, 41, 382
BstMCI CGRYCG 1 cut(s) 400
BstSFI CTRYAG 1 cut(s) 258
BstV1I GCAGC 3 cut(s) 44, 244, 313
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 2 cut(s) 41, 382
BstXI CCANNNNNNTGG 1 cut(s) 38
BstYI RGATCY 2 cut(s) 41, 382
BsuRI GGCC 1 cut(s) 183
BtsI GCAGTG 2 cut(s) 267, 369
BtsIMutI CAGTG 2 cut(s) 267, 369
CaiI CAGNNNCTG 1 cut(s) 368
Cfr13I GGNCC 1 cut(s) 181
Csp6I GTAC 2 cut(s) 223, 315
CviAII CATG 1 cut(s) 230
CviJI RGCY 5 cut(s) 183, 257, 305, 368, 375
CviKI_1 RGCY 5 cut(s) 183, 257, 305, 368, 375
CviQI GTAC 2 cut(s) 223, 315
DdeI CTNAG 1 cut(s) 369
DpnI GATC 3 cut(s) 21, 43, 384
DpnII GATC 3 cut(s) 19, 41, 382
FaeI CATG 1 cut(s) 233
FaiI YATR 8 cut(s) 36, 146, 148, 189, 191, 213, 231, 442
FatI CATG 1 cut(s) 229
Fnu4HI GCNGC 3 cut(s) 58, 258, 327
Fsp4HI GCNGC 3 cut(s) 58, 258, 327
FspBI CTAG 2 cut(s) 47, 330
GluI GCNGC 3 cut(s) 58, 258, 327
HaeIII GGCC 1 cut(s) 183
Hin1II CATG 1 cut(s) 233
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 4 cut(s) 52, 151, 160, 217
HpaI GTTAAC 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 94
Hpy188III TCNNGA 3 cut(s) 47, 71, 419
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 244, 328
HpyCH4III ACNGT 2 cut(s) 284, 401
HpyCH4V TGCA 3 cut(s) 260, 362, 380
HpyF3I CTNAG 1 cut(s) 369
Hsp92II CATG 1 cut(s) 233
KpnI GGTACC 1 cut(s) 318
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 19, 41, 382
LmnI GCTCC 1 cut(s) 103
LpnPI CCDG 5 cut(s) 56, 86, 140, 328, 432
Lsp1109I GCAGC 3 cut(s) 44, 244, 313
MaeI CTAG 2 cut(s) 47, 330
MalI GATC 3 cut(s) 21, 43, 384
MboI GATC 3 cut(s) 19, 41, 382
MboII GAAGA 2 cut(s) 278, 359
MflI RGATCY 2 cut(s) 41, 382
MluCI AATT 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 1 cut(s) 364
MseI TTAA 3 cut(s) 15, 93, 309
NdeII GATC 3 cut(s) 19, 41, 382
NlaIII CATG 1 cut(s) 233
NlaIV GGNNCC 1 cut(s) 316
PfeI GAWTC 4 cut(s) 52, 151, 160, 217
PkrI GCNGC 3 cut(s) 59, 259, 328
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 1 cut(s) 181
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 2 cut(s) 41, 382
RsaI GTAC 2 cut(s) 224, 316
RsaNI GTAC 2 cut(s) 223, 315
SaqAI TTAA 3 cut(s) 15, 93, 309
SatI GCNGC 3 cut(s) 58, 258, 327
Sau3AI GATC 3 cut(s) 19, 41, 382
Sau96I GGNCC 1 cut(s) 181
SetI ASST 2 cut(s) 259, 320
SfcI CTRYAG 1 cut(s) 258
Sse9I AATT 1 cut(s) 119
SspMI CTAG 2 cut(s) 47, 330
TaaI ACNGT 2 cut(s) 284, 401
TaqI TCGA 1 cut(s) 22
TasI AATT 1 cut(s) 119
TatI WGTACW 1 cut(s) 222
TfiI GAWTC 4 cut(s) 52, 151, 160, 217
Tru1I TTAA 3 cut(s) 15, 93, 309
Tru9I TTAA 3 cut(s) 15, 93, 309
TscAI CASTG 2 cut(s) 267, 369
TseI GCWGC 3 cut(s) 57, 257, 326
TspDTI ATGAA 3 cut(s) 17, 263, 310
TspRI CASTG 2 cut(s) 267, 369
XbaI TCTAGA 1 cut(s) 46
XspI CTAG 2 cut(s) 47, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.