RLG00000034336

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003073
Physical Location & Seq
Reverse (-)
40916639 .. 40918661
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034336

Sequence Viewer

Length: 513 bp
ATGGCAATTCTGCGCAGCCTTGTCTATGTTTTAAGGTATCTCAGCTTATTTGTTGAAAACCATTGCTCGGATCAAGATCTACGACCAAGGGCTTCAAGTCTGACGACGCGCAACGGTTTGGATGAGGAAGGCAACGAGGAATTTGAGGGCGTCGGTGATCTTTCTGGCCGGAGGGGTGGGGAATTTGAGGAAGACTTGGAGGGAGTGAGAGGAGAGGTAGGAATTGATGACAGAGAGGGCGGATTGCTGGTAGGAGCGGCCTTTGTAGAGGTTGACGTTGGAGATGGAGCCGCCGGGGCCGCCGCTGATTCTGATGGAGGGCGGCTGCTGGCGAAGCTGGAGTCGGAGTCACGATGGCGGAAGTTGTTGAGTGAACTTGATGGTGGCGATGGCGGTGGGTTCATCGACAGCTTGGGTCGTCGGCTGCCTCTCATATTGACCATGAAAGTTTGCAGGTGGGATTCCAATTTTGTTAGTGGATATGGGAGATTGAAATTTTGGATTAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.52

Weight (kDa)

4.67

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 444
Acc16I TGCGCA 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 444
AccBSI CCGCTC 1 cut(s) 257
AccII CGCG 1 cut(s) 109
AciI CCGC 8 cut(s) 240, 257, 291, 300, 303, 322, 358, 393
AclWI GGATC 1 cut(s) 78
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 3 cut(s) 140, 182, 494
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 3 cut(s) 56, 96, 493
AluBI AGCT 3 cut(s) 45, 337, 411
AluI AGCT 3 cut(s) 45, 337, 411
AlwI GGATC 1 cut(s) 78
AoxI GGCC 3 cut(s) 166, 258, 297
ApeKI GCWGC 3 cut(s) 15, 325, 424
ApoI RAATTY 3 cut(s) 140, 182, 494
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 2 cut(s) 15, 111
AspS9I GGNCC 1 cut(s) 297
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 27, 312, 411
BccI CCATC 5 cut(s) 278, 308, 348, 374, 383
BcnI CCSGG 1 cut(s) 295
BfuAI ACCTGC 1 cut(s) 444
BglI GCCNNNNNGGC 1 cut(s) 296
BglII AGATCT 1 cut(s) 76
BisI GCNGC 8 cut(s) 16, 258, 291, 300, 303, 323, 326, 425
BlsI GCNGC 8 cut(s) 17, 259, 292, 301, 304, 324, 327, 426
Bme1390I CCNGG 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 289, 298
BmrFI CCNGG 1 cut(s) 295
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 295
BsaBI GATNNNNATC 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 86, 294
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse3DI GCAATG 1 cut(s) 61
Bse8I GATNNNNATC 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 86, 294
BseGI GGATG 1 cut(s) 127
BseJI GATNNNNATC 1 cut(s) 75
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 61
BseMII CTCAG 1 cut(s) 55
BseRI GAGGAG 1 cut(s) 225
BseXI GCAGC 3 cut(s) 27, 312, 411
Bsh1236I CGCG 1 cut(s) 109
BshFI GGCC 3 cut(s) 168, 260, 299
BsiSI CCGG 2 cut(s) 169, 294
BslI CCNNNNNNNGG 1 cut(s) 67
BsnI GGCC 3 cut(s) 168, 260, 299
Bsp143I GATC 3 cut(s) 70, 76, 157
BspACI CCGC 8 cut(s) 240, 257, 291, 300, 303, 322, 358, 393
BspANI GGCC 3 cut(s) 168, 260, 299
BspCNI CTCAG 1 cut(s) 54
BspFNI CGCG 1 cut(s) 109
BspLI GGNNCC 2 cut(s) 289, 298
BspMI ACCTGC 1 cut(s) 444
BspPI GGATC 1 cut(s) 78
BsrBI CCGCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 86, 294
BssMI GATC 3 cut(s) 70, 76, 157
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 116
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 1 cut(s) 109
BstHHI GCGC 2 cut(s) 15, 111
BstKTI GATC 3 cut(s) 73, 79, 160
BstMBI GATC 3 cut(s) 70, 76, 157
BstMWI GCNNNNNNNGC 3 cut(s) 296, 299, 334
BstSCI CCNGG 1 cut(s) 293
BstUI CGCG 1 cut(s) 109
BstV1I GCAGC 3 cut(s) 27, 312, 411
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 3 cut(s) 168, 260, 299
BtgZI GCGATG 1 cut(s) 402
BtsCI GGATG 1 cut(s) 127
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 2 cut(s) 15, 111
Cfr13I GGNCC 1 cut(s) 297
CseI GACGC 2 cut(s) 115, 139
CviAII CATG 1 cut(s) 442
DdeI CTNAG 1 cut(s) 41
DpnI GATC 3 cut(s) 72, 78, 159
DpnII GATC 3 cut(s) 70, 76, 157
EaeI YGGCCR 1 cut(s) 166
EciI GGCGGA 2 cut(s) 255, 373
Eco130I CCWWGG 1 cut(s) 86
EcoT14I CCWWGG 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 86
FaeI CATG 1 cut(s) 445
FaiI YATR 4 cut(s) 27, 434, 443, 483
FatI CATG 1 cut(s) 441
Fnu4HI GCNGC 8 cut(s) 16, 258, 291, 300, 303, 323, 326, 425
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 8 cut(s) 16, 258, 291, 300, 303, 323, 326, 425
FspI TGCGCA 1 cut(s) 14
GlaI GCGC 2 cut(s) 14, 110
GluI GCNGC 8 cut(s) 16, 258, 291, 300, 303, 323, 326, 425
GsuI CTGGAG 1 cut(s) 359
HaeIII GGCC 3 cut(s) 168, 260, 299
HapII CCGG 2 cut(s) 169, 294
HgaI GACGC 2 cut(s) 115, 139
HhaI GCGC 2 cut(s) 15, 111
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 445
Hin6I GCGC 2 cut(s) 13, 109
HinP1I GCGC 2 cut(s) 13, 109
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 4 cut(s) 308, 341, 347, 461
HpaII CCGG 2 cut(s) 169, 294
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 274, 374
Hpy188I TCNGA 4 cut(s) 70, 102, 313, 346
Hpy188III TCNNGA 2 cut(s) 74, 351
Hpy8I GTNNAC 2 cut(s) 274, 374
Hpy99I CGWCG 3 cut(s) 109, 155, 423
HpyAV CCTTC 1 cut(s) 122
HpyCH4III ACNGT 1 cut(s) 116
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 299, 334
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 445
HspAI GCGC 2 cut(s) 13, 109
Kzo9I GATC 3 cut(s) 70, 76, 157
LmnI GCTCC 2 cut(s) 254, 287
LpnPI CCDG 7 cut(s) 150, 182, 233, 307, 314, 323, 439
Lsp1109I GCAGC 3 cut(s) 27, 312, 411
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 348
MalI GATC 3 cut(s) 72, 78, 159
MbiI CCGCTC 1 cut(s) 257
MboI GATC 3 cut(s) 70, 76, 157
MboII GAAGA 1 cut(s) 203
MflI RGATCY 1 cut(s) 76
MluCI AATT 6 cut(s) 6, 140, 182, 222, 466, 494
MlyI GAGTC 2 cut(s) 350, 356
MmeI TCCRAC 2 cut(s) 259, 324
MseI TTAA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 2 cut(s) 169, 294
MspR9I CCNGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 109
MwoI GCNNNNNNNGC 3 cut(s) 296, 299, 334
NciI CCSGG 1 cut(s) 295
NdeII GATC 3 cut(s) 70, 76, 157
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 2 cut(s) 289, 298
NmuCI GTSAC 1 cut(s) 348
NsbI TGCGCA 1 cut(s) 14
PaqCI CACCTGC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 308, 461
PkrI GCNGC 8 cut(s) 17, 259, 292, 301, 304, 324, 327, 426
PleI GAGTC 2 cut(s) 349, 355
PpsI GAGTC 2 cut(s) 349, 355
PspN4I GGNNCC 2 cut(s) 289, 298
PspPI GGNCC 1 cut(s) 297
PsuI RGATCY 1 cut(s) 76
SaqAI TTAA 1 cut(s) 32
SatI GCNGC 8 cut(s) 16, 258, 291, 300, 303, 323, 326, 425
Sau3AI GATC 3 cut(s) 70, 76, 157
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 2 cut(s) 350, 356
ScrFI CCNGG 1 cut(s) 295
SetI ASST 8 cut(s) 38, 47, 219, 273, 279, 339, 413, 458
Sse9I AATT 6 cut(s) 6, 140, 182, 222, 466, 494
SsiI CCGC 8 cut(s) 240, 257, 291, 300, 303, 322, 358, 393
StyD4I CCNGG 1 cut(s) 293
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 1 cut(s) 116
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 405
TasI AATT 6 cut(s) 6, 140, 182, 222, 466, 494
TauI GCSGC 5 cut(s) 260, 293, 302, 305, 325
TfiI GAWTC 2 cut(s) 308, 461
Tru1I TTAA 1 cut(s) 32
Tru9I TTAA 1 cut(s) 32
TseFI GTSAC 1 cut(s) 348
TseI GCWGC 3 cut(s) 15, 325, 424
Tsp45I GTSAC 1 cut(s) 348
TspDTI ATGAA 2 cut(s) 391, 458
XapI RAATTY 3 cut(s) 140, 182, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.