Rh5DG220200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003073
Physical Location & Seq
Forward (+)
24795174 .. 24803438
8265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG220200.1

Sequence Viewer

Length: 615 bp
ATGGCTCCTAGCGGTTCAGCTGCCGCCATAGCTGGAATGTGCCGCCGTCGTAATGGCGGAGGGGGCAACTCCAGCTCCGGAGGCATGGACGGCAGCGGTGCGGCTCTGGTGCCAGAAACAACATGCTCAGGTCTCTGTAATATTAGTCTTCTAACTGCATATGAGAAACAACATTTAAGTTGTTTGAGCATTGATCATGGAACTTTTGTTGTACGTTTCGTTCATGATAGCCATGGCCGATCGAGACTTCGTGGCTATCTGAATTGCTTGGCGCTCAACCAGAGGGGAGATCAACTCGTCTGCCATCGTGGGCTTGGTTCTGTTTGCAAGAACACTCAATTGAATCCTAATAAGTTTATTACTCAGTCAATCTGGGCAAACATCTTCTTTGCGCTTAGGTTCTTTGCCTTTTGTTCCCGACTAGATAGTAAGTATTGCCGGAGCCACCGCAGTTGCAGTATATTTCTTGCAGAAGTATTAAGACAACAATGTGCGCTTGGGCTATTGAGTACTCATTGGTTAATTGACTTCTTCATCACGGTCTTTTCCACCGCCACCTGCTGGAGATCAGGGAAACACAGTGCTACATGCACTAGAGGCAAAAAGGCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.19

Weight (kDa)

9.57

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 566
Acc36I ACCTGC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 109
AccB7I CCANNNNNTGG 1 cut(s) 561
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 8 cut(s) 12, 24, 43, 57, 96, 101, 448, 552
AcoI YGGCCR 1 cut(s) 235
AfaI GTAC 2 cut(s) 213, 511
AfiI CCNNNNNNNGG 1 cut(s) 561
AgsI TTSAA 1 cut(s) 343
AluBI AGCT 3 cut(s) 20, 32, 75
AluI AGCT 3 cut(s) 20, 32, 75
Alw26I GTCTC 2 cut(s) 137, 238
Aor13HI TCCGGA 1 cut(s) 77
AoxI GGCC 1 cut(s) 235
ApeKI GCWGC 2 cut(s) 20, 93
AspLEI GCGC 3 cut(s) 274, 394, 496
BanI GGYRCC 1 cut(s) 109
BbsI GAAGAC 1 cut(s) 140
BbvI GCAGC 2 cut(s) 7, 105
BccI CCATC 1 cut(s) 312
BceAI ACGGC 2 cut(s) 30, 106
BclI TGATCA 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 137, 238
BfaI CTAG 3 cut(s) 9, 422, 594
BfoI RGCGCY 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 566
BisI GCNGC 5 cut(s) 21, 24, 43, 94, 102
BlsI GCNGC 5 cut(s) 22, 25, 44, 95, 103
BmcAI AGTACT 1 cut(s) 511
BmiI GGNNCC 3 cut(s) 6, 111, 443
BpiI GAAGAC 1 cut(s) 140
BplI GAGNNNNNCTC 2 cut(s) 279, 311
BpmI CTGGAG 2 cut(s) 55, 583
Bpu10I CCTNAGC 2 cut(s) 127, 395
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 232
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 1 cut(s) 561
BseAI TCCGGA 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 232
BseLI CCNNNNNNNGG 1 cut(s) 561
BseMII CTCAG 2 cut(s) 141, 377
BseXI GCAGC 2 cut(s) 7, 105
Bsh1285I CGRYCG 1 cut(s) 242
BshFI GGCC 1 cut(s) 237
BshNI GGYRCC 1 cut(s) 109
BsiEI CGRYCG 1 cut(s) 242
BsiSI CCGG 2 cut(s) 78, 439
BslI CCNNNNNNNGG 1 cut(s) 561
BsmAI GTCTC 2 cut(s) 137, 238
BsnI GGCC 1 cut(s) 237
Bso31I GGTCTC 1 cut(s) 137
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 4 cut(s) 193, 239, 289, 566
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 8 cut(s) 12, 24, 43, 57, 96, 101, 448, 552
BspANI GGCC 1 cut(s) 237
BspCNI CTCAG 2 cut(s) 140, 376
BspEI TCCGGA 1 cut(s) 77
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 3 cut(s) 6, 111, 443
BspMI ACCTGC 1 cut(s) 566
BspT107I GGYRCC 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 4 cut(s) 193, 239, 289, 566
BssT1I CCWWGG 1 cut(s) 232
Bst4CI ACNGT 2 cut(s) 541, 581
BstDEI CTNAG 3 cut(s) 127, 363, 395
BstDSI CCRYGG 1 cut(s) 232
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 3 cut(s) 274, 394, 496
BstKTI GATC 4 cut(s) 196, 242, 292, 569
BstMAI GTCTC 2 cut(s) 137, 238
BstMBI GATC 4 cut(s) 193, 239, 289, 566
BstMCI CGRYCG 1 cut(s) 242
BstMWI GCNNNNNNNGC 6 cut(s) 29, 63, 72, 81, 90, 597
BstNSI RCATGY 2 cut(s) 126, 591
BstV1I GCAGC 2 cut(s) 7, 105
BstV2I GAAGAC 1 cut(s) 140
BsuRI GGCC 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 586
BveI ACCTGC 1 cut(s) 566
CciI TCATGA 1 cut(s) 223
CfoI GCGC 3 cut(s) 274, 394, 496
Csp6I GTAC 2 cut(s) 212, 510
CspCI CAANNNNNGTGG 2 cut(s) 434, 469
CviAII CATG 6 cut(s) 85, 123, 197, 224, 233, 588
CviQI GTAC 2 cut(s) 212, 510
DdeI CTNAG 3 cut(s) 127, 363, 395
DpnI GATC 4 cut(s) 195, 241, 291, 568
DpnII GATC 4 cut(s) 193, 239, 289, 566
EaeI YGGCCR 1 cut(s) 235
EciI GGCGGA 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 232
Eco31I GGTCTC 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 232
FaeI CATG 6 cut(s) 88, 126, 200, 227, 236, 591
FatI CATG 6 cut(s) 84, 122, 196, 223, 232, 587
FauNDI CATATG 1 cut(s) 160
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 5 cut(s) 21, 24, 43, 94, 102
Fsp4HI GCNGC 5 cut(s) 21, 24, 43, 94, 102
FspBI CTAG 3 cut(s) 9, 422, 594
GlaI GCGC 3 cut(s) 273, 393, 495
GluI GCNGC 5 cut(s) 21, 24, 43, 94, 102
GsuI CTGGAG 2 cut(s) 55, 583
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 1 cut(s) 237
HapII CCGG 2 cut(s) 78, 439
HhaI GCGC 3 cut(s) 274, 394, 496
Hin1II CATG 6 cut(s) 88, 126, 200, 227, 236, 591
Hin6I GCGC 3 cut(s) 272, 392, 494
HinP1I GCGC 3 cut(s) 272, 392, 494
HinfI GANTC 1 cut(s) 343
HpaII CCGG 2 cut(s) 78, 439
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 4 cut(s) 78, 224, 243, 417
Hpy99I CGWCG 1 cut(s) 51
HpyCH4III ACNGT 2 cut(s) 541, 581
HpyCH4IV ACGT 1 cut(s) 214
HpyCH4V TGCA 5 cut(s) 158, 327, 456, 470, 591
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 63, 72, 81, 90, 597
HpyF3I CTNAG 3 cut(s) 127, 363, 395
HpySE526I ACGT 1 cut(s) 214
Hsp92II CATG 6 cut(s) 88, 126, 200, 227, 236, 591
HspAI GCGC 3 cut(s) 272, 392, 494
Kpn2I TCCGGA 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 193
Kzo9I GATC 4 cut(s) 193, 239, 289, 566
LmnI GCTCC 3 cut(s) 10, 80, 441
Lsp1109I GCAGC 2 cut(s) 7, 105
MaeI CTAG 3 cut(s) 9, 422, 594
MaeII ACGT 1 cut(s) 214
MalI GATC 4 cut(s) 195, 241, 291, 568
MboI GATC 4 cut(s) 193, 239, 289, 566
MboII GAAGA 3 cut(s) 140, 376, 523
MfeI CAATTG 1 cut(s) 338
MluCI AATT 3 cut(s) 262, 338, 522
MnlI CCTC 4 cut(s) 53, 74, 276, 590
MroI TCCGGA 1 cut(s) 77
MseI TTAA 3 cut(s) 176, 479, 521
MspA1I CMGCKG 2 cut(s) 20, 96
MspI CCGG 2 cut(s) 78, 439
MunI CAATTG 1 cut(s) 338
MwoI GCNNNNNNNGC 6 cut(s) 29, 63, 72, 81, 90, 597
NcoI CCATGG 1 cut(s) 232
NdeI CATATG 1 cut(s) 160
NdeII GATC 4 cut(s) 193, 239, 289, 566
NlaIII CATG 6 cut(s) 88, 126, 200, 227, 236, 591
NlaIV GGNNCC 3 cut(s) 6, 111, 443
NspI RCATGY 2 cut(s) 126, 591
PagI TCATGA 1 cut(s) 223
PaqCI CACCTGC 1 cut(s) 566
PfeI GAWTC 1 cut(s) 343
PflMI CCANNNNNTGG 1 cut(s) 561
PkrI GCNGC 5 cut(s) 22, 25, 44, 95, 103
Ple19I CGATCG 1 cut(s) 242
PspN4I GGNNCC 3 cut(s) 6, 111, 443
PsrI GAACNNNNNNTAC 2 cut(s) 204, 236
PvuI CGATCG 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 2 cut(s) 213, 511
RsaNI GTAC 2 cut(s) 212, 510
SaqAI TTAA 3 cut(s) 176, 479, 521
SatI GCNGC 5 cut(s) 21, 24, 43, 94, 102
Sau3AI GATC 4 cut(s) 193, 239, 289, 566
ScaI AGTACT 1 cut(s) 511
SetI ASST 7 cut(s) 22, 34, 77, 133, 217, 401, 560
Sse9I AATT 3 cut(s) 262, 338, 522
SsiI CCGC 8 cut(s) 12, 24, 43, 57, 96, 101, 448, 552
SspI AATATT 1 cut(s) 142
SspMI CTAG 3 cut(s) 9, 422, 594
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 2 cut(s) 541, 581
TaiI ACGT 1 cut(s) 217
TaqI TCGA 1 cut(s) 242
TasI AATT 3 cut(s) 262, 338, 522
TatI WGTACW 1 cut(s) 509
TauI GCSGC 3 cut(s) 26, 45, 104
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 3 cut(s) 176, 479, 521
Tru9I TTAA 3 cut(s) 176, 479, 521
TscAI CASTG 1 cut(s) 586
TseI GCWGC 2 cut(s) 20, 93
TspDTI ATGAA 2 cut(s) 212, 523
TspRI CASTG 1 cut(s) 586
Van91I CCANNNNNTGG 1 cut(s) 561
XceI RCATGY 2 cut(s) 126, 591
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XspI CTAG 3 cut(s) 9, 422, 594
ZrmI AGTACT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.