Rh2BG308300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003073
Physical Location & Seq
Reverse (-)
35876738 .. 35877259
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG308300.1

Sequence Viewer

Length: 477 bp
ATGCATAAACCCTCCCCCTTGTTAAGCTCCATCTCTAGCCTAAATGCGCAGGCTCTAGCGCGTCAAATTGGACGCATTTCTCTTGTGCTTCCTAAGAAAGTGGTTGAGGATAAATCGCAGATGAAGGAGATTAATATTTCTAAAGTGAAGTGCAGCGCTTCTATGGCATTGGTGACGTATACGAAATGCACCAAACAGCTTGATCTTCTCCAAAAGGAGGGGGAACTGTTGGTGTCTCCTCTGAAGAAGAAGAGGAAGAGGGTGGGCAGACTAGTTAGGAGCCATAATAAACCCAAGATGGTGGATATCAACCCTTGGAAGCTCAAGGCGGGGGTGGGTACTGTGAAGCAACCCATGACTAAGCAGCTTCCAAAGGCTTTGGAGGGAGATGTGAAACGAGCTCTGGAGCTTGCCATTGATGAGGAATCCCCTATAGAGAGTCCATGGATGTTGAGGCTAAGATACCAGTACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.87

Weight (kDa)

10.11

Isoelectric Point (pI)

67.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 48
AccI GTMKAC 1 cut(s) 179
AccII CGCG 1 cut(s) 61
AciI CCGC 1 cut(s) 329
AcuI CTGAAG 1 cut(s) 263
AfaI GTAC 2 cut(s) 340, 470
AfeI AGCGCT 1 cut(s) 157
AfiI CCNNNNNNNGG 1 cut(s) 217
AflIII ACRYGT 1 cut(s) 471
AhlI ACTAGT 1 cut(s) 271
AluBI AGCT 6 cut(s) 27, 199, 322, 367, 401, 409
AluI AGCT 6 cut(s) 27, 199, 322, 367, 401, 409
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 1 cut(s) 240
Aor51HI AGCGCT 1 cut(s) 157
ApeKI GCWGC 2 cut(s) 153, 364
AseI ATTAAT 1 cut(s) 132
AspLEI GCGC 3 cut(s) 49, 61, 158
AsuHPI GGTGA 1 cut(s) 184
BanII GRGCYC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 2 cut(s) 165, 376
BccI CCATC 2 cut(s) 38, 292
BcoDI GTCTC 1 cut(s) 240
BcuI ACTAGT 1 cut(s) 271
BfaI CTAG 3 cut(s) 36, 56, 272
BfmI CTRYAG 1 cut(s) 432
BfoI RGCGCY 1 cut(s) 159
BisI GCNGC 2 cut(s) 154, 365
BlsI GCNGC 2 cut(s) 155, 366
BmiI GGNNCC 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 314, 443
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 466
BseDI CCNNGG 2 cut(s) 314, 443
BseGI GGATG 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 217
BseNI ACTGG 1 cut(s) 466
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 2 cut(s) 165, 376
BsgI GTGCAG 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 403
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 1 cut(s) 202
Bsp19I CCATGG 1 cut(s) 443
BspACI CCGC 1 cut(s) 329
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 281
BsrI ACTGG 1 cut(s) 466
BssECI CCNNGG 2 cut(s) 314, 443
BssMI GATC 1 cut(s) 202
BssNAI GTATAC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 314, 443
Bst1107I GTATAC 1 cut(s) 180
Bst4CI ACNGT 2 cut(s) 228, 343
Bst6I CTCTTC 2 cut(s) 245, 251
BstC8I GCNNGC 2 cut(s) 51, 411
BstDEI CTNAG 3 cut(s) 93, 360, 458
BstDSI CCRYGG 1 cut(s) 443
BstF5I GGATG 1 cut(s) 453
BstFNI CGCG 1 cut(s) 61
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 3 cut(s) 49, 61, 158
BstKTI GATC 1 cut(s) 205
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 475
BstSFI CTRYAG 1 cut(s) 432
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 165, 376
BstXI CCANNNNNNTGG 1 cut(s) 301
BstZ17I GTATAC 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 443
BtsCI GGATG 1 cut(s) 453
Cac8I GCNNGC 2 cut(s) 51, 411
CfoI GCGC 3 cut(s) 49, 61, 158
CseI GACGC 2 cut(s) 50, 81
Csp6I GTAC 2 cut(s) 339, 469
CviAII CATG 3 cut(s) 355, 444, 472
CviQI GTAC 2 cut(s) 339, 469
DdeI CTNAG 3 cut(s) 93, 360, 458
DpnI GATC 1 cut(s) 204
DpnII GATC 1 cut(s) 202
Eam1104I CTCTTC 2 cut(s) 245, 251
EarI CTCTTC 2 cut(s) 245, 251
Ecl136II GAGCTC 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 314, 443
Eco24I GRGCYC 1 cut(s) 403
Eco32I GATATC 1 cut(s) 307
Eco47III AGCGCT 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 263
EcoICRI GAGCTC 1 cut(s) 401
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 314, 443
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 403
ErhI CCWWGG 2 cut(s) 314, 443
FaeI CATG 3 cut(s) 358, 447, 475
FaiI YATR 8 cut(s) 6, 164, 180, 285, 356, 434, 445, 473
FatI CATG 3 cut(s) 354, 443, 471
FauI CCCGC 1 cut(s) 322
FblI GTMKAC 1 cut(s) 179
Fnu4HI GCNGC 2 cut(s) 154, 365
FokI GGATG 1 cut(s) 460
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 154, 365
FspBI CTAG 3 cut(s) 36, 56, 272
FspI TGCGCA 1 cut(s) 48
GlaI GCGC 3 cut(s) 48, 60, 157
GluI GCNGC 2 cut(s) 154, 365
GsuI CTGGAG 1 cut(s) 425
HaeII RGCGCY 1 cut(s) 159
HgaI GACGC 2 cut(s) 50, 81
HhaI GCGC 3 cut(s) 49, 61, 158
Hin1II CATG 3 cut(s) 358, 447, 475
Hin6I GCGC 3 cut(s) 47, 59, 156
HinP1I GCGC 3 cut(s) 47, 59, 156
HinfI GANTC 2 cut(s) 425, 439
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 1 cut(s) 180
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 2 cut(s) 228, 343
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 3 cut(s) 4, 153, 189
HpyF10VI GCNNNNNNNGC 1 cut(s) 164
HpyF3I CTNAG 3 cut(s) 93, 360, 458
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 3 cut(s) 358, 447, 475
HspAI GCGC 3 cut(s) 47, 59, 156
Kzo9I GATC 1 cut(s) 202
LmnI GCTCC 3 cut(s) 32, 279, 406
LpnPI CCDG 2 cut(s) 35, 389
Lsp1109I GCAGC 2 cut(s) 165, 376
MaeI CTAG 3 cut(s) 36, 56, 272
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 172
MalI GATC 1 cut(s) 204
MboI GATC 1 cut(s) 202
MboII GAAGA 5 cut(s) 197, 256, 259, 262, 268
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 1 cut(s) 66
MlyI GAGTC 1 cut(s) 448
MnlI CCTC 9 cut(s) 22, 100, 211, 246, 249, 252, 376, 415, 447
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 23, 132
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 164
NcoI CCATGG 1 cut(s) 443
NdeII GATC 1 cut(s) 202
NlaIII CATG 3 cut(s) 358, 447, 475
NlaIV GGNNCC 1 cut(s) 281
NmuCI GTSAC 1 cut(s) 172
NsbI TGCGCA 1 cut(s) 48
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 475
PciI ACATGT 1 cut(s) 471
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 2 cut(s) 155, 366
PleI GAGTC 1 cut(s) 447
PpsI GAGTC 1 cut(s) 447
PscI ACATGT 1 cut(s) 471
PshBI ATTAAT 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 403
PspN4I GGNNCC 1 cut(s) 281
RsaI GTAC 2 cut(s) 340, 470
RsaNI GTAC 2 cut(s) 339, 469
SacI GAGCTC 1 cut(s) 403
SaqAI TTAA 2 cut(s) 23, 132
SatI GCNGC 2 cut(s) 154, 365
Sau3AI GATC 1 cut(s) 202
SchI GAGTC 1 cut(s) 448
SduI GDGCHC 1 cut(s) 403
SetI ASST 7 cut(s) 29, 179, 201, 324, 369, 403, 411
SfcI CTRYAG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SpeI ACTAGT 1 cut(s) 271
Sse9I AATT 1 cut(s) 66
SsiI CCGC 1 cut(s) 329
SspI AATATT 1 cut(s) 136
SspMI CTAG 3 cut(s) 36, 56, 272
SstI GAGCTC 1 cut(s) 403
StyI CCWWGG 2 cut(s) 314, 443
TaaI ACNGT 2 cut(s) 228, 343
TaiI ACGT 1 cut(s) 179
TasI AATT 1 cut(s) 66
TatI WGTACW 1 cut(s) 468
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 2 cut(s) 23, 132
Tru9I TTAA 2 cut(s) 23, 132
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 2 cut(s) 153, 364
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 137
VspI ATTAAT 1 cut(s) 132
XceI RCATGY 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 179
XspI CTAG 3 cut(s) 36, 56, 272
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.