Rmu_sc0000247.1_g000074

B-Box-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000247.1
OG0001915
Physical Location & Seq
Reverse (-)
381000 .. 381828
829 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
atgaaagagtgcgagctgtgtggagtgagagctcggatgtactgcgaggcggaccgcgcgagtttatgctgggactgcgacaagaaagttcacggagctaactttctggtggcgaagcatccgagagttcttctctgccatgtgtgccagtcgcagacgccgtggacgagttgcggagccaagctgacgccgacggtgtcggtctgccagagttgcgttggaagctgtagaaagattgacctgaacgaggagcaagaaagcgaggcagagaatgaagaagaagaagaagaagattattatgatgatgaagatgaagaagatgaggaggaagaggatgagaaccaggtggttccatgggcatgctgttcttactcaccggcacagcgtccaccagcagtgagcggttcggatagtgaagccgagatttcggtgtcgaagcgactgcgagagaatgtagatgtggattattctgatgatgaggtagggtttgatgggacgtcgtcgtttggtggttcaatgagaccgttgaaaaggccgaaagtgggtgaagagaagaatggtgacggagatgatggcggtgaatcggagtggagagcaacggcgatcatgagctcaattcagaggctggaaaaggaagtgatcaacggcgctggtaatgcgtccaccgcggttcttaggatttgcaaactcagtagagatgagagcagccattga

Protein Analysis

237

Amino Acids

26.26

Weight (kDa)

4.42

Isoelectric Point (pI)

58.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 43 2.5e-06 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 500
AccBSI CCGCTC 1 cut(s) 402
AccII CGCG 3 cut(s) 57, 59, 668
AciI CCGC 7 cut(s) 50, 55, 174, 402, 576, 666, 668
AcyI GRCGYC 3 cut(s) 158, 188, 497
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 247, 542
AgsI TTSAA 2 cut(s) 516, 529
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 6 cut(s) 16, 32, 98, 184, 225, 612
AluI AGCT 6 cut(s) 16, 32, 98, 184, 225, 612
Alw21I GWGCWC 2 cut(s) 34, 614
Alw26I GTCTC 1 cut(s) 514
AlwNI CAGNNNCTG 1 cut(s) 625
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 1 cut(s) 705
AspLEI GCGC 2 cut(s) 59, 650
AspS9I GGNCC 1 cut(s) 52
AsuHPI GGTGA 4 cut(s) 366, 557, 572, 590
AvaII GGWCC 1 cut(s) 52
BanII GRGCYC 2 cut(s) 34, 614
Bbv12I GWGCWC 2 cut(s) 34, 614
BccI CCATC 2 cut(s) 485, 566
BceAI ACGGC 3 cut(s) 145, 615, 661
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 1 cut(s) 344
BclI TGATCA 1 cut(s) 639
BcoDI GTCTC 1 cut(s) 514
BfmI CTRYAG 1 cut(s) 226
BfoI RGCGCY 1 cut(s) 651
BisI GCNGC 1 cut(s) 706
BlsI GCNGC 1 cut(s) 707
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 2 cut(s) 178, 351
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BsaHI GRCGYC 3 cut(s) 158, 188, 497
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 3 cut(s) 161, 353, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 542
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 344
BseDI CCNNGG 3 cut(s) 161, 353, 666
BseGI GGATG 3 cut(s) 42, 118, 340
BseLI CCNNNNNNNGG 2 cut(s) 247, 542
BseMII CTCAG 1 cut(s) 703
BseNI ACTGG 1 cut(s) 148
BseRI GAGGAG 2 cut(s) 263, 338
BseYI CCCAGC 1 cut(s) 69
Bsh1236I CGCG 3 cut(s) 57, 59, 668
BshFI GGCC 1 cut(s) 535
BsiHKAI GWGCWC 2 cut(s) 34, 614
BsiSI CCGG 1 cut(s) 377
BslFI GGGAC 2 cut(s) 86, 508
BslI CCNNNNNNNGG 2 cut(s) 247, 542
BsmAI GTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 86, 508
BsnI GGCC 1 cut(s) 535
Bso31I GGTCTC 1 cut(s) 514
Bsp1286I GDGCHC 2 cut(s) 34, 614
Bsp143I GATC 2 cut(s) 603, 639
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 7 cut(s) 50, 55, 174, 402, 576, 666, 668
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 1 cut(s) 702
BspFNI CGCG 3 cut(s) 57, 59, 668
BspHI TCATGA 1 cut(s) 606
BspLI GGNNCC 2 cut(s) 178, 351
BspTNI GGTCTC 1 cut(s) 514
BsrBI CCGCTC 1 cut(s) 402
BsrFI RCCGGY 1 cut(s) 376
BsrI ACTGG 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 3 cut(s) 161, 353, 666
BssMI GATC 2 cut(s) 603, 639
BssNI GRCGYC 3 cut(s) 158, 188, 497
BssT1I CCWWGG 1 cut(s) 353
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 2 cut(s) 196, 525
Bst6I CTCTTC 2 cut(s) 324, 543
BstACI GRCGYC 3 cut(s) 158, 188, 497
BstC8I GCNNGC 2 cut(s) 14, 361
BstDEI CTNAG 2 cut(s) 674, 689
BstDSI CCRYGG 3 cut(s) 161, 353, 666
BstENI CCTNNNNNAGG 1 cut(s) 245
BstF5I GGATG 3 cut(s) 42, 118, 340
BstFNI CGCG 3 cut(s) 57, 59, 668
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 2 cut(s) 59, 650
BstKTI GATC 2 cut(s) 606, 642
BstMAI GTCTC 1 cut(s) 514
BstMBI GATC 2 cut(s) 603, 639
BstMWI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 656, 665
BstNI CCWGG 1 cut(s) 344
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 3 cut(s) 57, 59, 668
BsuRI GGCC 1 cut(s) 535
BtgI CCRYGG 3 cut(s) 161, 353, 666
BtsCI GGATG 3 cut(s) 42, 118, 340
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 2 cut(s) 14, 361
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 1 cut(s) 606
CfoI GCGC 2 cut(s) 59, 650
Cfr10I RCCGGY 1 cut(s) 376
Cfr13I GGNCC 1 cut(s) 52
Cfr42I CCGCGG 1 cut(s) 669
CpoI CGGWCCG 1 cut(s) 52
CseI GACGC 4 cut(s) 166, 196, 374, 648
CsiI ACCWGGT 1 cut(s) 342
Csp6I GTAC 1 cut(s) 40
CspI CGGWCCG 1 cut(s) 52
CviAII CATG 4 cut(s) 140, 354, 360, 607
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 674, 689
DpnI GATC 2 cut(s) 605, 641
DpnII GATC 2 cut(s) 603, 639
Eam1104I CTCTTC 2 cut(s) 324, 543
EarI CTCTTC 2 cut(s) 324, 543
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 2 cut(s) 32, 612
Eco130I CCWWGG 1 cut(s) 353
Eco24I GRGCYC 2 cut(s) 34, 614
Eco31I GGTCTC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 52
Eco53kI GAGCTC 2 cut(s) 32, 612
EcoICRI GAGCTC 2 cut(s) 32, 612
EcoNI CCTNNNNNAGG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 353
EcoT38I GRGCYC 2 cut(s) 34, 614
ErhI CCWWGG 1 cut(s) 353
FaeI CATG 4 cut(s) 143, 357, 363, 610
FaiI YATR 6 cut(s) 67, 141, 300, 355, 361, 608
FaqI GGGAC 2 cut(s) 86, 508
FatI CATG 4 cut(s) 139, 353, 359, 606
FbaI TGATCA 1 cut(s) 639
Fnu4HI GCNGC 1 cut(s) 706
FokI GGATG 3 cut(s) 49, 105, 347
FriOI GRGCYC 2 cut(s) 34, 614
Fsp4HI GCNGC 1 cut(s) 706
GlaI GCGC 2 cut(s) 58, 649
GluI GCNGC 1 cut(s) 706
GsaI CCCAGC 1 cut(s) 73
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 1 cut(s) 535
HapII CCGG 1 cut(s) 377
HgaI GACGC 4 cut(s) 166, 196, 374, 648
HhaI GCGC 2 cut(s) 59, 650
Hin1I GRCGYC 3 cut(s) 158, 188, 497
Hin1II CATG 4 cut(s) 143, 357, 363, 610
Hin6I GCGC 2 cut(s) 57, 648
HinP1I GCGC 2 cut(s) 57, 648
HinfI GANTC 1 cut(s) 581
HpaII CCGG 1 cut(s) 377
HphI GGTGA 4 cut(s) 366, 557, 572, 590
Hpy166II GTNNAC 4 cut(s) 91, 165, 389, 663
Hpy188I TCNGA 6 cut(s) 36, 123, 409, 472, 586, 621
Hpy188III TCNNGA 1 cut(s) 607
Hpy8I GTNNAC 4 cut(s) 91, 165, 389, 663
Hpy99I CGWCG 3 cut(s) 196, 502, 505
HpyCH4III ACNGT 2 cut(s) 196, 525
HpyCH4IV ACGT 1 cut(s) 497
HpyCH4V TGCA 1 cut(s) 684
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 656, 665
HpyF3I CTNAG 2 cut(s) 674, 689
HpySE526I ACGT 1 cut(s) 497
Hsp92I GRCGYC 3 cut(s) 158, 188, 497
Hsp92II CATG 4 cut(s) 143, 357, 363, 610
HspAI GCGC 2 cut(s) 57, 648
Ksp22I TGATCA 1 cut(s) 639
KspI CCGCGG 1 cut(s) 669
Kzo9I GATC 2 cut(s) 603, 639
LmnI GCTCC 3 cut(s) 95, 176, 250
LweI GCATC 1 cut(s) 127
MabI ACCWGGT 1 cut(s) 342
MaeII ACGT 1 cut(s) 497
MaeIII GTNAC 1 cut(s) 560
MalI GATC 2 cut(s) 605, 641
MbiI CCGCTC 1 cut(s) 402
MboI GATC 2 cut(s) 603, 639
MhlI GDGCHC 2 cut(s) 34, 614
MluCI AATT 1 cut(s) 615
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 8 cut(s) 40, 241, 256, 316, 319, 325, 472, 615
MslI CAYNNNNRTG 1 cut(s) 358
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 3 cut(s) 57, 59, 668
MwoI GCNNNNNNNGC 7 cut(s) 56, 75, 144, 213, 222, 656, 665
NcoI CCATGG 1 cut(s) 353
NdeII GATC 2 cut(s) 603, 639
NlaIII CATG 4 cut(s) 143, 357, 363, 610
NlaIV GGNNCC 2 cut(s) 178, 351
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 560
NspI RCATGY 1 cut(s) 363
PaeI GCATGC 1 cut(s) 363
PagI TCATGA 1 cut(s) 606
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 164
PfeI GAWTC 1 cut(s) 581
PflFI GACNNNGTC 2 cut(s) 196, 499
PkrI GCNGC 1 cut(s) 707
Psp124BI GAGCTC 2 cut(s) 34, 614
Psp6I CCWGG 1 cut(s) 342
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 178, 351
PspPI GGNCC 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 625
PsyI GACNNNGTC 2 cut(s) 196, 499
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 358
Rsr2I CGGWCCG 1 cut(s) 52
RsrII CGGWCCG 1 cut(s) 52
SacI GAGCTC 2 cut(s) 34, 614
SacII CCGCGG 1 cut(s) 669
SatI GCNGC 1 cut(s) 706
Sau3AI GATC 2 cut(s) 603, 639
Sau96I GGNCC 1 cut(s) 52
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 2 cut(s) 34, 614
SexAI ACCWGGT 1 cut(s) 342
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 226
Sfr303I CCGCGG 1 cut(s) 669
SgrBI CCGCGG 1 cut(s) 669
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 358
SphI GCATGC 1 cut(s) 363
Sse9I AATT 1 cut(s) 615
SsiI CCGC 7 cut(s) 50, 55, 174, 402, 576, 666, 668
SstI GAGCTC 2 cut(s) 34, 614
StyD4I CCNGG 1 cut(s) 342
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 2 cut(s) 196, 525
TaiI ACGT 1 cut(s) 500
TaqI TCGA 1 cut(s) 434
TaqII GACCGA 1 cut(s) 190
TasI AATT 1 cut(s) 615
TatI WGTACW 1 cut(s) 39
TfiI GAWTC 1 cut(s) 581
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 1 cut(s) 705
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 4 cut(s) 17, 288, 321, 327
TspGWI ACGGA 2 cut(s) 108, 579
TspRI CASTG 1 cut(s) 402
Tth111I GACNNNGTC 2 cut(s) 196, 499
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 245
XceI RCATGY 1 cut(s) 363
XcmI CCANNNNNNNNNTGG 1 cut(s) 215
ZraI GACGTC 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.