Rmu_sc0000524.1_g000007

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000524.1
OG0002220
Physical Location & Seq
Forward (+)
58135 .. 60324
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
atgaaacagaggaagatattggatccggccagctatgatcagaaagttcagaagctccaccatcacaggagccgagtaacaatgtcaatgttgatgcacaaacagcatagcaggattggaaccaatgctatgaagctttcgaatatcgaatggaagttgttaatggaactggcagctgtattggtgatcagtgtagtatttgccaggagaagtttgaggaagacgaagaagatgaatacgagacaccaaacaggtcaagctcaccttgatatcgaatctggaggccaccagttaccagccgctccaccgcagcaacctgctggtaacaatgctggacagccacgacggcccaaggattggagtggatactatgaaatttttagtcatctgaaagttggtgagtgtagtatttgcctgggagagtttgaggaaccagatgtgtgcgcgagacttcgtaagtgtaagcatacgtatcaccgggaatgcattgatctgtggctacctaaatcgtgtccgatttgtcgtcaaaacggagtataa

Protein Analysis

179

Amino Acids

20.57

Weight (kDa)

9.76

Isoelectric Point (pI)

67.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 134 - 175 1e-10 Ring finger domain
zf-RING_11 PF17123 134 - 163 1.1e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 134 - 174 2.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 134 - 176 1.7e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 135 - 166 1e-06 RING-type zinc-finger
zf-C3HC4 PF00097 135 - 174 4.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 325
AccB7I CCANNNNNTGG 1 cut(s) 357
AccBSI CCGCTC 1 cut(s) 302
AccII CGCG 1 cut(s) 446
AciI CCGC 2 cut(s) 300, 308
AclWI GGATC 2 cut(s) 17, 30
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 357
AjnI CCWGG 2 cut(s) 203, 414
AluBI AGCT 5 cut(s) 33, 55, 136, 176, 260
AluI AGCT 5 cut(s) 33, 55, 136, 176, 260
Alw26I GTCTC 2 cut(s) 235, 442
AlwI GGATC 2 cut(s) 17, 30
AoxI GGCC 3 cut(s) 27, 283, 347
ApeKI GCWGC 2 cut(s) 173, 310
ApoI RAATTY 1 cut(s) 375
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 1 cut(s) 348
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 4 cut(s) 196, 254, 410, 467
AsuII TTCGAA 1 cut(s) 140
BamHI GGATCC 1 cut(s) 22
BbsI GAAGAC 1 cut(s) 227
BbvI GCAGC 2 cut(s) 185, 322
BccI CCATC 1 cut(s) 69
BceAI ACGGC 1 cut(s) 362
BciT130I CCWGG 2 cut(s) 205, 416
BciVI GTATCC 1 cut(s) 359
BclI TGATCA 2 cut(s) 37, 186
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 235, 442
BfuAI ACCTGC 1 cut(s) 325
BfuI GTATCC 1 cut(s) 359
BglI GCCNNNNNGGC 1 cut(s) 346
BisI GCNGC 3 cut(s) 174, 300, 311
BlsI GCNGC 3 cut(s) 175, 301, 312
Bme1390I CCNGG 3 cut(s) 205, 416, 479
BmgT120I GGNCC 1 cut(s) 348
BmiI GGNNCC 4 cut(s) 24, 71, 121, 432
BmrFI CCNGG 3 cut(s) 205, 416, 479
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 300
Bpu14I TTCGAA 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 479
BsaAI YACGTR 1 cut(s) 471
BsaJI CCNNGG 2 cut(s) 351, 415
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse1I ACTGG 2 cut(s) 174, 289
BseBI CCWGG 2 cut(s) 205, 416
BseDI CCNNGG 2 cut(s) 351, 415
BseLI CCNNNNNNNGG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 174, 289
BseXI GCAGC 2 cut(s) 185, 322
Bsh1236I CGCG 1 cut(s) 446
BshFI GGCC 3 cut(s) 29, 285, 349
BsiSI CCGG 2 cut(s) 26, 478
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 2 cut(s) 235, 442
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 3 cut(s) 29, 285, 349
Bsp119I TTCGAA 1 cut(s) 140
Bsp143I GATC 4 cut(s) 22, 37, 186, 490
BspACI CCGC 2 cut(s) 300, 308
BspANI GGCC 3 cut(s) 29, 285, 349
BspFNI CGCG 1 cut(s) 446
BspLI GGNNCC 4 cut(s) 24, 71, 121, 432
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 2 cut(s) 17, 30
BspT104I TTCGAA 1 cut(s) 140
BsrBI CCGCTC 1 cut(s) 302
BsrI ACTGG 2 cut(s) 174, 289
BssECI CCNNGG 2 cut(s) 351, 415
BssMI GATC 4 cut(s) 22, 37, 186, 490
BssT1I CCWWGG 1 cut(s) 351
Bst2UI CCWGG 2 cut(s) 205, 416
BstBAI YACGTR 1 cut(s) 471
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 31
BstFNI CGCG 1 cut(s) 446
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 4 cut(s) 25, 40, 189, 493
BstMAI GTCTC 2 cut(s) 235, 442
BstMBI GATC 4 cut(s) 22, 37, 186, 490
BstMWI GCNNNNNNNGC 2 cut(s) 103, 346
BstNI CCWGG 2 cut(s) 205, 416
BstSCI CCNGG 3 cut(s) 203, 414, 477
BstSNI TACGTA 1 cut(s) 471
BstUI CGCG 1 cut(s) 446
BstV1I GCAGC 2 cut(s) 185, 322
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
BsuI GTATCC 1 cut(s) 359
BsuRI GGCC 3 cut(s) 29, 285, 349
BtsIMutI CAGTG 1 cut(s) 196
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 446
Cfr13I GGNCC 1 cut(s) 348
DpnI GATC 4 cut(s) 24, 39, 188, 492
DpnII GATC 4 cut(s) 22, 37, 186, 490
EaeI YGGCCR 1 cut(s) 27
Eco105I TACGTA 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 351
Eco32I GATATC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 203, 414
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 488
ErhI CCWWGG 1 cut(s) 351
FaiI YATR 6 cut(s) 36, 108, 131, 372, 468, 538
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FbaI TGATCA 2 cut(s) 37, 186
Fnu4HI GCNGC 3 cut(s) 174, 300, 311
Fsp4HI GCNGC 3 cut(s) 174, 300, 311
GlaI GCGC 1 cut(s) 445
GluI GCNGC 3 cut(s) 174, 300, 311
GsuI CTGGAG 1 cut(s) 300
HaeIII GGCC 3 cut(s) 29, 285, 349
HapII CCGG 2 cut(s) 26, 478
HhaI GCGC 1 cut(s) 446
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 1 cut(s) 275
HpaII CCGG 2 cut(s) 26, 478
HphI GGTGA 4 cut(s) 196, 254, 410, 467
Hpy188I TCNGA 4 cut(s) 42, 51, 390, 516
Hpy188III TCNNGA 1 cut(s) 279
Hpy99I CGWCG 1 cut(s) 348
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 2 cut(s) 97, 486
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 346
HpySE526I ACGT 1 cut(s) 470
HspAI GCGC 1 cut(s) 444
Ksp22I TGATCA 2 cut(s) 37, 186
Kzo9I GATC 4 cut(s) 22, 37, 186, 490
LmnI GCTCC 3 cut(s) 60, 69, 307
Lsp1109I GCAGC 2 cut(s) 185, 322
LweI GCATC 1 cut(s) 84
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 3 cut(s) 76, 291, 323
MalI GATC 4 cut(s) 24, 39, 188, 492
MbiI CCGCTC 1 cut(s) 302
MboI GATC 4 cut(s) 22, 37, 186, 490
MboII GAAGA 4 cut(s) 25, 232, 238, 241
MflI RGATCY 1 cut(s) 22
MluCI AATT 1 cut(s) 375
MnlI CCTC 4 cut(s) 3, 210, 275, 421
Mph1103I ATGCAT 1 cut(s) 488
MseI TTAA 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 2 cut(s) 26, 478
MspR9I CCNGG 3 cut(s) 205, 416, 479
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 2 cut(s) 205, 416
MvnI CGCG 1 cut(s) 446
MwoI GCNNNNNNNGC 2 cut(s) 103, 346
NciI CCSGG 1 cut(s) 479
NdeII GATC 4 cut(s) 22, 37, 186, 490
NlaIV GGNNCC 4 cut(s) 24, 71, 121, 432
NmeAIII GCCGAG 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 488
NspV TTCGAA 1 cut(s) 140
PctI GAATGC 1 cut(s) 488
PfeI GAWTC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 357
PkrI GCNGC 3 cut(s) 175, 301, 312
Ppu21I YACGTR 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 203, 414
PspGI CCWGG 2 cut(s) 203, 414
PspN4I GGNNCC 4 cut(s) 24, 71, 121, 432
PspPI GGNCC 1 cut(s) 348
PsuI RGATCY 1 cut(s) 22
PvuII CAGCTG 1 cut(s) 176
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 3 cut(s) 174, 300, 311
Sau3AI GATC 4 cut(s) 22, 37, 186, 490
Sau96I GGNCC 1 cut(s) 348
ScrFI CCNGG 3 cut(s) 205, 416, 479
SfaNI GCATC 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 140
SnaBI TACGTA 1 cut(s) 471
Sse9I AATT 1 cut(s) 375
SsiI CCGC 2 cut(s) 300, 308
StyD4I CCNGG 3 cut(s) 203, 414, 477
StyI CCWWGG 1 cut(s) 351
TaiI ACGT 1 cut(s) 473
TaqI TCGA 3 cut(s) 140, 147, 273
TasI AATT 1 cut(s) 375
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 1 cut(s) 196
TseI GCWGC 2 cut(s) 173, 310
TspDTI ATGAA 4 cut(s) 17, 146, 248, 387
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 357
XapI RAATTY 1 cut(s) 375
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.