Transferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 77 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 746 |
| AclWI | GGATC | 1 cut(s) | 221 |
| AcsI | RAATTY | 3 cut(s) | 404, 419, 625 |
| AcuI | CTGAAG | 2 cut(s) | 282, 570 |
| AfaI | GTAC | 3 cut(s) | 156, 321, 397 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 699, 746 |
| AflIII | ACRYGT | 1 cut(s) | 66 |
| AgsI | TTSAA | 5 cut(s) | 317, 517, 548, 656, 661 |
| AjnI | CCWGG | 1 cut(s) | 733 |
| AluBI | AGCT | 5 cut(s) | 18, 142, 485, 508, 832 |
| AluI | AGCT | 5 cut(s) | 18, 142, 485, 508, 832 |
| Alw21I | GWGCWC | 3 cut(s) | 455, 510, 834 |
| Alw26I | GTCTC | 4 cut(s) | 71, 310, 368, 432 |
| AlwI | GGATC | 1 cut(s) | 221 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 485 |
| AoxI | GGCC | 2 cut(s) | 125, 737 |
| ApoI | RAATTY | 3 cut(s) | 404, 419, 625 |
| AseI | ATTAAT | 1 cut(s) | 696 |
| AspS9I | GGNCC | 1 cut(s) | 737 |
| AsuHPI | GGTGA | 3 cut(s) | 397, 719, 785 |
| BanII | GRGCYC | 2 cut(s) | 510, 834 |
| BbsI | GAAGAC | 1 cut(s) | 245 |
| Bbv12I | GWGCWC | 3 cut(s) | 455, 510, 834 |
| BbvCI | CCTCAGC | 1 cut(s) | 486 |
| BccI | CCATC | 4 cut(s) | 376, 436, 550, 755 |
| BciT130I | CCWGG | 1 cut(s) | 735 |
| BcoDI | GTCTC | 4 cut(s) | 71, 310, 368, 432 |
| BfuAI | ACCTGC | 1 cut(s) | 77 |
| BmcAI | AGTACT | 2 cut(s) | 156, 321 |
| Bme1390I | CCNGG | 1 cut(s) | 735 |
| BmgT120I | GGNCC | 1 cut(s) | 737 |
| BmrFI | CCNGG | 1 cut(s) | 735 |
| BmsI | GCATC | 1 cut(s) | 28 |
| BpiI | GAAGAC | 1 cut(s) | 245 |
| Bpu10I | CCTNAGC | 1 cut(s) | 486 |
| Bsa29I | ATCGAT | 1 cut(s) | 778 |
| BsaAI | YACGTR | 1 cut(s) | 94 |
| BsaI | GGTCTC | 2 cut(s) | 71, 310 |
| BsaJI | CCNNGG | 4 cut(s) | 81, 162, 300, 734 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 750, 780 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 699, 746 |
| Bse1I | ACTGG | 1 cut(s) | 128 |
| Bse3DI | GCAATG | 1 cut(s) | 849 |
| BseBI | CCWGG | 1 cut(s) | 735 |
| BseCI | ATCGAT | 1 cut(s) | 778 |
| BseDI | CCNNGG | 4 cut(s) | 81, 162, 300, 734 |
| BseGI | GGATG | 2 cut(s) | 387, 447 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 699, 746 |
| BseMI | GCAATG | 1 cut(s) | 849 |
| BseMII | CTCAG | 3 cut(s) | 300, 321, 477 |
| BseNI | ACTGG | 1 cut(s) | 128 |
| BseYI | CCCAGC | 1 cut(s) | 18 |
| BshFI | GGCC | 2 cut(s) | 127, 739 |
| BshVI | ATCGAT | 1 cut(s) | 778 |
| BsiHKAI | GWGCWC | 3 cut(s) | 455, 510, 834 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 699, 746 |
| BsmAI | GTCTC | 4 cut(s) | 71, 310, 368, 432 |
| BsmBI | CGTCTC | 1 cut(s) | 368 |
| BsnI | GGCC | 2 cut(s) | 127, 739 |
| Bso31I | GGTCTC | 2 cut(s) | 71, 310 |
| Bsp1286I | GDGCHC | 3 cut(s) | 455, 510, 834 |
| Bsp1407I | TGTACA | 1 cut(s) | 395 |
| Bsp143I | GATC | 4 cut(s) | 213, 354, 775, 779 |
| Bsp19I | CCATGG | 1 cut(s) | 81 |
| BspANI | GGCC | 2 cut(s) | 127, 739 |
| BspCNI | CTCAG | 3 cut(s) | 299, 320, 478 |
| BspDI | ATCGAT | 1 cut(s) | 778 |
| BspMI | ACCTGC | 1 cut(s) | 77 |
| BspPI | GGATC | 1 cut(s) | 221 |
| BspTNI | GGTCTC | 2 cut(s) | 71, 310 |
| BsrDI | GCAATG | 1 cut(s) | 849 |
| BsrGI | TGTACA | 1 cut(s) | 395 |
| BsrI | ACTGG | 1 cut(s) | 128 |
| BssECI | CCNNGG | 4 cut(s) | 81, 162, 300, 734 |
| BssMI | GATC | 4 cut(s) | 213, 354, 775, 779 |
| BssT1I | CCWWGG | 3 cut(s) | 81, 162, 300 |
| Bst2UI | CCWGG | 1 cut(s) | 735 |
| Bst4CI | ACNGT | 2 cut(s) | 457, 790 |
| Bst6I | CTCTTC | 1 cut(s) | 543 |
| BstAUI | TGTACA | 1 cut(s) | 395 |
| BstBAI | YACGTR | 1 cut(s) | 94 |
| BstC8I | GCNNGC | 1 cut(s) | 129 |
| BstDEI | CTNAG | 5 cut(s) | 138, 286, 307, 486, 724 |
| BstDSI | CCRYGG | 1 cut(s) | 81 |
| BstF5I | GGATG | 2 cut(s) | 387, 447 |
| BstKTI | GATC | 4 cut(s) | 216, 357, 778, 782 |
| BstMAI | GTCTC | 4 cut(s) | 71, 310, 368, 432 |
| BstMBI | GATC | 4 cut(s) | 213, 354, 775, 779 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 47, 605, 736, 846 |
| BstNI | CCWGG | 1 cut(s) | 735 |
| BstNSI | RCATGY | 1 cut(s) | 70 |
| BstSCI | CCNGG | 1 cut(s) | 733 |
| BstV2I | GAAGAC | 1 cut(s) | 245 |
| Bsu15I | ATCGAT | 1 cut(s) | 778 |
| BsuRI | GGCC | 2 cut(s) | 127, 739 |
| BsuTUI | ATCGAT | 1 cut(s) | 778 |
| BtgI | CCRYGG | 1 cut(s) | 81 |
| BtsCI | GGATG | 2 cut(s) | 387, 447 |
| BtsIMutI | CAGTG | 1 cut(s) | 12 |
| BveI | ACCTGC | 1 cut(s) | 77 |
| Cac8I | GCNNGC | 1 cut(s) | 129 |
| CaiI | CAGNNNCTG | 1 cut(s) | 485 |
| Cfr13I | GGNCC | 1 cut(s) | 737 |
| ClaI | ATCGAT | 1 cut(s) | 778 |
| Csp6I | GTAC | 3 cut(s) | 155, 320, 396 |
| CviAII | CATG | 4 cut(s) | 67, 82, 254, 541 |
| CviJI | RGCY | 8 cut(s) | 18, 50, 127, 142, 485, 508, 739, 832 |
| CviKI_1 | RGCY | 8 cut(s) | 18, 50, 127, 142, 485, 508, 739, 832 |
| CviQI | GTAC | 3 cut(s) | 155, 320, 396 |
| DdeI | CTNAG | 5 cut(s) | 138, 286, 307, 486, 724 |
| DpnI | GATC | 4 cut(s) | 215, 356, 777, 781 |
| DpnII | GATC | 4 cut(s) | 213, 354, 775, 779 |
| Eam1104I | CTCTTC | 1 cut(s) | 543 |
| EarI | CTCTTC | 1 cut(s) | 543 |
| Ecl136II | GAGCTC | 2 cut(s) | 508, 832 |
| Eco130I | CCWWGG | 3 cut(s) | 81, 162, 300 |
| Eco24I | GRGCYC | 2 cut(s) | 510, 834 |
| Eco31I | GGTCTC | 2 cut(s) | 71, 310 |
| Eco53kI | GAGCTC | 2 cut(s) | 508, 832 |
| Eco57I | CTGAAG | 2 cut(s) | 282, 570 |
| EcoICRI | GAGCTC | 2 cut(s) | 508, 832 |
| EcoRII | CCWGG | 1 cut(s) | 733 |
| EcoT14I | CCWWGG | 3 cut(s) | 81, 162, 300 |
| EcoT22I | ATGCAT | 1 cut(s) | 43 |
| EcoT38I | GRGCYC | 2 cut(s) | 510, 834 |
| ErhI | CCWWGG | 3 cut(s) | 81, 162, 300 |
| Esp3I | CGTCTC | 1 cut(s) | 368 |
| FaeI | CATG | 4 cut(s) | 70, 85, 257, 544 |
| FatI | CATG | 4 cut(s) | 66, 81, 253, 540 |
| FauNDI | CATATG | 2 cut(s) | 335, 601 |
| FokI | GGATG | 2 cut(s) | 394, 454 |
| FriOI | GRGCYC | 2 cut(s) | 510, 834 |
| GsaI | CCCAGC | 1 cut(s) | 22 |
| HaeIII | GGCC | 2 cut(s) | 127, 739 |
| Hin1II | CATG | 4 cut(s) | 70, 85, 257, 544 |
| HinfI | GANTC | 3 cut(s) | 194, 388, 425 |
| HphI | GGTGA | 3 cut(s) | 397, 719, 785 |
| Hpy188I | TCNGA | 2 cut(s) | 496, 589 |
| Hpy188III | TCNNGA | 3 cut(s) | 119, 260, 653 |
| HpyAV | CCTTC | 3 cut(s) | 67, 655, 707 |
| HpyCH4III | ACNGT | 2 cut(s) | 457, 790 |
| HpyCH4IV | ACGT | 2 cut(s) | 93, 114 |
| HpyCH4V | TGCA | 5 cut(s) | 41, 112, 131, 329, 683 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 47, 605, 736, 846 |
| HpyF3I | CTNAG | 5 cut(s) | 138, 286, 307, 486, 724 |
| HpySE526I | ACGT | 2 cut(s) | 93, 114 |
| Hsp92II | CATG | 4 cut(s) | 70, 85, 257, 544 |
| Kzo9I | GATC | 4 cut(s) | 213, 354, 775, 779 |
| LmnI | GCTCC | 3 cut(s) | 147, 450, 846 |
| LpnPI | CCDG | 9 cut(s) | 4, 72, 109, 132, 141, 273, 720, 747, 813 |
| LweI | GCATC | 1 cut(s) | 28 |
| MaeII | ACGT | 2 cut(s) | 93, 114 |
| MaeIII | GTNAC | 4 cut(s) | 89, 166, 385, 784 |
| MalI | GATC | 4 cut(s) | 215, 356, 777, 781 |
| MboI | GATC | 4 cut(s) | 213, 354, 775, 779 |
| MboII | GAAGA | 4 cut(s) | 250, 539, 560, 576 |
| MfeI | CAATTG | 2 cut(s) | 809, 841 |
| MhlI | GDGCHC | 3 cut(s) | 455, 510, 834 |
| MlyI | GAGTC | 2 cut(s) | 382, 434 |
| MnlI | CCTC | 4 cut(s) | 61, 372, 481, 748 |
| Mph1103I | ATGCAT | 1 cut(s) | 43 |
| MseI | TTAA | 6 cut(s) | 183, 273, 459, 696, 768, 856 |
| MslI | CAYNNNNRTG | 1 cut(s) | 65 |
| MspR9I | CCNGG | 1 cut(s) | 735 |
| MunI | CAATTG | 2 cut(s) | 809, 841 |
| MvaI | CCWGG | 1 cut(s) | 735 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 47, 605, 736, 846 |
| NcoI | CCATGG | 1 cut(s) | 81 |
| NdeI | CATATG | 2 cut(s) | 335, 601 |
| NdeII | GATC | 4 cut(s) | 213, 354, 775, 779 |
| NlaIII | CATG | 4 cut(s) | 70, 85, 257, 544 |
| NmuCI | GTSAC | 2 cut(s) | 385, 784 |
| NsiI | ATGCAT | 1 cut(s) | 43 |
| NspI | RCATGY | 1 cut(s) | 70 |
| PciI | ACATGT | 1 cut(s) | 66 |
| PfeI | GAWTC | 1 cut(s) | 194 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 746 |
| PleI | GAGTC | 2 cut(s) | 382, 433 |
| PpsI | GAGTC | 2 cut(s) | 382, 433 |
| Ppu21I | YACGTR | 1 cut(s) | 94 |
| PscI | ACATGT | 1 cut(s) | 66 |
| PshBI | ATTAAT | 1 cut(s) | 696 |
| Psp124BI | GAGCTC | 2 cut(s) | 510, 834 |
| Psp6I | CCWGG | 1 cut(s) | 733 |
| PspFI | CCCAGC | 1 cut(s) | 18 |
| PspGI | CCWGG | 1 cut(s) | 733 |
| PspPI | GGNCC | 1 cut(s) | 737 |
| PstNI | CAGNNNCTG | 1 cut(s) | 485 |
| RsaI | GTAC | 3 cut(s) | 156, 321, 397 |
| RsaNI | GTAC | 3 cut(s) | 155, 320, 396 |
| RseI | CAYNNNNRTG | 1 cut(s) | 65 |
| SacI | GAGCTC | 2 cut(s) | 510, 834 |
| SaqAI | TTAA | 6 cut(s) | 183, 273, 459, 696, 768, 856 |
| Sau3AI | GATC | 4 cut(s) | 213, 354, 775, 779 |
| Sau96I | GGNCC | 1 cut(s) | 737 |
| ScaI | AGTACT | 2 cut(s) | 156, 321 |
| SchI | GAGTC | 2 cut(s) | 382, 434 |
| ScrFI | CCNGG | 1 cut(s) | 735 |
| SduI | GDGCHC | 3 cut(s) | 455, 510, 834 |
| SfaNI | GCATC | 1 cut(s) | 28 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 65 |
| SspI | AATATT | 1 cut(s) | 565 |
| SstI | GAGCTC | 2 cut(s) | 510, 834 |
| StyD4I | CCNGG | 1 cut(s) | 733 |
| StyI | CCWWGG | 3 cut(s) | 81, 162, 300 |
| TaaI | ACNGT | 2 cut(s) | 457, 790 |
| TaiI | ACGT | 2 cut(s) | 96, 117 |
| TaqI | TCGA | 1 cut(s) | 778 |
| TatI | WGTACW | 3 cut(s) | 154, 319, 395 |
| TfiI | GAWTC | 1 cut(s) | 194 |
| Tru1I | TTAA | 6 cut(s) | 183, 273, 459, 696, 768, 856 |
| Tru9I | TTAA | 6 cut(s) | 183, 273, 459, 696, 768, 856 |
| TscAI | CASTG | 1 cut(s) | 12 |
| TseFI | GTSAC | 2 cut(s) | 385, 784 |
| Tsp45I | GTSAC | 2 cut(s) | 385, 784 |
| TspDTI | ATGAA | 5 cut(s) | 49, 242, 251, 546, 726 |
| TspGWI | ACGGA | 2 cut(s) | 187, 398 |
| TspRI | CASTG | 1 cut(s) | 12 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 746 |
| VspI | ATTAAT | 1 cut(s) | 696 |
| XapI | RAATTY | 3 cut(s) | 404, 419, 625 |
| XceI | RCATGY | 1 cut(s) | 70 |
| ZrmI | AGTACT | 2 cut(s) | 156, 321 |
| Zsp2I | ATGCAT | 1 cut(s) | 43 |