Rmu_sc0004550.1_g000015

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004550.1
OG0001786
Physical Location & Seq
Forward (+)
40593 .. 46120
5528 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1437 bp
atgcagggtctgtacagtaggggagcacggcggttcatggtggcgggtcttccaccgatcggatgccttccgcttcaacggacactgagcagcatcatcaatctgcgccctcagcgcctgtgcgtcacgcagcaaaacatagactgtcagacctacaactccaagctccaggcccttctctcaagtttgcaaaccacactgccactccctagttcaaggctagtctattttgacatctacaaccccatcatggacatgtacaacaacccagataaatatggatttgtgcatatacatgaaggatgctgtggaactgggatgtttgagttggggcctctgtgcacagagttttcgcggacgtgccccgacgcgtcgaagtatcttttctgggatgcagttcatcctactgaagcagcatttttcgtcattgctgactatgctcataagactgtcttgcagtaccttactctcctttctcggtctgaagcctccggcgaaacgtcaccggtgtccgaattcatcacaatggtggacaaagtctcggagtttagtcaagatcaagaagaaaggtttttgtactatgtatctgttgcagaggagttggcgcaagagttgagttcttcaaatgggaaattgaaatcacttattgatgacttgagaaatgaagtggattctatcaggactttcaggaatgtacattctatggaataccagaagcttctgatggaagaggagcagatggcagaagaattgaagctgtgtctggaaattggaaaggacaggttccaatcacaagcttatgatccaacttttgttgggtcaatcaaagaagtgcaatgtaccgactttcagaggtcctcctttgatgaaaaatgcatagagtccattaagcctctgatggatgagaagcagaaggcagaggagtctttgaaaattgaaaaggagatgacgcgatcccaggctaatgaactggcttcccttagatctgccttaaatgaaaagtgcaccgagtggcaggagcttctaagggaagagaaggagaaggcagagaagttaatgctacttttggaaattgaaaaggagagaacacaggctgatgaactggcttctattagatccatctttgatgaaaagtgcgtcgagtaccagaagcttctaatggatgaaaggcagaaggtagaggattcaatgcgactattgaaaattcaaaaggggttaacgcaatcacaagctaatcaactggttttcagatccgccacagatgaatttttggggaaagaaaaccaggcttctaaaaaacttttggaagttttaaaggcagaattgaaggatgtgaagaacagagaactgtgtgcttgtggtcaagtctcaggagcaacaaacaaacgcaaaagagagaagaacgagaaagaggaggaagctaacaatgaacagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

478

Amino Acids

54.95

Weight (kDa)

5.12

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 8 - 144 1.9e-11 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 355, 371, 952
AciI CCGC 5 cut(s) 31, 44, 71, 355, 1254
AclWI GGATC 4 cut(s) 797, 948, 1110, 1245
AcsI RAATTY 3 cut(s) 515, 1203, 1265
AcuI CTGAAG 2 cut(s) 429, 504
AdeI CACNNNGTG 1 cut(s) 1011
AfaI GTAC 7 cut(s) 14, 260, 461, 578, 696, 841, 1145
AfiI CCNNNNNNNGG 2 cut(s) 59, 250
AflIII ACRYGT 2 cut(s) 255, 369
AgeI ACCGGT 1 cut(s) 505
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 3 cut(s) 168, 957, 1284
AleI CACNNNNGTG 1 cut(s) 527
AluBI AGCT 8 cut(s) 166, 718, 757, 797, 1021, 1153, 1232, 1421
AluI AGCT 8 cut(s) 166, 718, 757, 797, 1021, 1153, 1232, 1421
Alw21I GWGCWC 3 cut(s) 28, 344, 1007
Alw26I GTCTC 2 cut(s) 544, 1372
Alw44I GTGCAC 2 cut(s) 340, 1003
AlwI GGATC 4 cut(s) 797, 948, 1110, 1245
AlwNI CAGNNNCTG 2 cut(s) 10, 118
AoxI GGCC 2 cut(s) 171, 332
ApaLI GTGCAC 2 cut(s) 340, 1003
ApeKI GCWGC 3 cut(s) 90, 130, 413
ApoI RAATTY 3 cut(s) 515, 1203, 1265
AsiGI ACCGGT 1 cut(s) 505
AspLEI GCGC 3 cut(s) 108, 117, 607
AspS9I GGNCC 3 cut(s) 172, 332, 855
AsuHPI GGTGA 1 cut(s) 495
AvaII GGWCC 1 cut(s) 855
BaeGI GKGCMC 3 cut(s) 344, 365, 1007
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 3 cut(s) 28, 344, 1007
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 102, 142, 425
BccI CCATC 5 cut(s) 254, 718, 733, 892, 1127
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 3 cut(s) 170, 959, 1286
BcoDI GTCTC 2 cut(s) 544, 1372
BfaI CTAG 2 cut(s) 210, 221
BfoI RGCGCY 1 cut(s) 118
BglII AGATCT 1 cut(s) 983
BisI GCNGC 3 cut(s) 91, 131, 414
BlsI GCNGC 3 cut(s) 92, 132, 415
Bme1390I CCNGG 3 cut(s) 170, 959, 1286
Bme18I GGWCC 1 cut(s) 855
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 172, 332, 855
BmiI GGNNCC 2 cut(s) 333, 785
BmrFI CCNGG 3 cut(s) 170, 959, 1286
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 4 cut(s) 53, 102, 293, 382
BmuI ACTGGG 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 1 cut(s) 152
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 166, 676
BsaJI CCNNGG 1 cut(s) 957
BsaWI WCCGGW 1 cut(s) 505
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 250
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 4 cut(s) 319, 975, 1107, 1245
Bse3DI GCAATG 2 cut(s) 426, 842
BseBI CCWGG 3 cut(s) 170, 959, 1286
BseDI CCNNGG 1 cut(s) 957
BseGI GGATG 8 cut(s) 68, 308, 324, 397, 400, 907, 1168, 1336
BseLI CCNNNNNNNGG 2 cut(s) 59, 250
BseMI GCAATG 2 cut(s) 426, 842
BseMII CTCAG 3 cut(s) 77, 125, 1383
BseNI ACTGG 4 cut(s) 319, 975, 1107, 1245
BseRI GAGGAG 4 cut(s) 611, 746, 935, 1427
BseSI GKGCMC 3 cut(s) 344, 365, 1007
BseXI GCAGC 3 cut(s) 102, 142, 425
Bsh1236I CGCG 3 cut(s) 355, 371, 952
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 173, 334
BshTI ACCGGT 1 cut(s) 505
BsiEI CGRYCG 1 cut(s) 60
BsiHKAI GWGCWC 3 cut(s) 28, 344, 1007
BsiSI CCGG 2 cut(s) 492, 506
BslI CCNNNNNNNGG 2 cut(s) 59, 250
BsmAI GTCTC 2 cut(s) 544, 1372
BsnI GGCC 2 cut(s) 173, 334
Bsp1286I GDGCHC 4 cut(s) 28, 344, 365, 1007
Bsp1407I TGTACA 3 cut(s) 12, 258, 694
Bsp143I GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
BspACI CCGC 5 cut(s) 31, 44, 71, 355, 1254
BspANI GGCC 2 cut(s) 173, 334
BspCNI CTCAG 3 cut(s) 78, 124, 1382
BspFNI CGCG 3 cut(s) 355, 371, 952
BspLI GGNNCC 2 cut(s) 333, 785
BspPI GGATC 4 cut(s) 797, 948, 1110, 1245
BsrDI GCAATG 2 cut(s) 426, 842
BsrFI RCCGGY 1 cut(s) 505
BsrGI TGTACA 3 cut(s) 12, 258, 694
BsrI ACTGG 4 cut(s) 319, 975, 1107, 1245
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 957
BssMI GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
Bst2UI CCWGG 3 cut(s) 170, 959, 1286
Bst4CI ACNGT 5 cut(s) 17, 146, 451, 1350, 1434
Bst6I CTCTTC 2 cut(s) 723, 1026
BstAUI TGTACA 3 cut(s) 12, 258, 694
BstDEI CTNAG 5 cut(s) 86, 111, 980, 1025, 1369
BstF5I GGATG 8 cut(s) 68, 308, 324, 397, 400, 907, 1168, 1336
BstFNI CGCG 3 cut(s) 355, 371, 952
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 3 cut(s) 108, 117, 607
BstKTI GATC 7 cut(s) 60, 559, 805, 956, 986, 1118, 1253
BstMAI GTCTC 2 cut(s) 544, 1372
BstMBI GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 3 cut(s) 112, 114, 437
BstNI CCWGG 3 cut(s) 170, 959, 1286
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 3 cut(s) 168, 957, 1284
BstSLI GKGCMC 3 cut(s) 344, 365, 1007
BstUI CGCG 3 cut(s) 355, 371, 952
BstV1I GCAGC 3 cut(s) 102, 142, 425
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 3 cut(s) 983, 1115, 1250
BstYI RGATCY 3 cut(s) 983, 1115, 1250
BsuRI GGCC 2 cut(s) 173, 334
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 8 cut(s) 68, 308, 324, 397, 400, 907, 1168, 1336
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 2 cut(s) 83, 197
CaiI CAGNNNCTG 2 cut(s) 10, 118
CfoI GCGC 3 cut(s) 108, 117, 607
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 3 cut(s) 172, 332, 855
CseI GACGC 5 cut(s) 112, 360, 377, 958, 1126
Csp6I GTAC 7 cut(s) 13, 259, 460, 577, 695, 840, 1144
CspAI ACCGGT 1 cut(s) 505
CviAII CATG 4 cut(s) 37, 250, 256, 296
CviQI GTAC 7 cut(s) 13, 259, 460, 577, 695, 840, 1144
DdeI CTNAG 5 cut(s) 86, 111, 980, 1025, 1369
DpnI GATC 7 cut(s) 59, 558, 804, 955, 985, 1117, 1252
DpnII GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
DraI TTTAAA 1 cut(s) 1314
DraIII CACNNNGTG 1 cut(s) 1011
Eam1104I CTCTTC 2 cut(s) 723, 1026
EarI CTCTTC 2 cut(s) 723, 1026
EciI GGCGGA 1 cut(s) 1243
Eco47I GGWCC 1 cut(s) 855
Eco57I CTGAAG 2 cut(s) 429, 504
EcoO109I RGGNCCY 3 cut(s) 172, 332, 855
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 3 cut(s) 168, 957, 1284
EcoT22I ATGCAT 1 cut(s) 878
FaeI CATG 4 cut(s) 40, 253, 259, 299
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FatI CATG 4 cut(s) 36, 249, 255, 295
FauI CCCGC 1 cut(s) 37
Fnu4HI GCNGC 3 cut(s) 91, 131, 414
FokI GGATG 8 cut(s) 75, 315, 331, 387, 404, 914, 1175, 1343
Fsp4HI GCNGC 3 cut(s) 91, 131, 414
FspBI CTAG 2 cut(s) 210, 221
GlaI GCGC 3 cut(s) 107, 116, 606
GluI GCNGC 3 cut(s) 91, 131, 414
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 2 cut(s) 173, 334
HapII CCGG 2 cut(s) 492, 506
HgaI GACGC 5 cut(s) 112, 360, 377, 958, 1126
HhaI GCGC 3 cut(s) 108, 117, 607
Hin1II CATG 4 cut(s) 40, 253, 259, 299
Hin6I GCGC 3 cut(s) 106, 115, 605
HinP1I GCGC 3 cut(s) 106, 115, 605
HincII GTYRAC 1 cut(s) 1218
HindII GTYRAC 1 cut(s) 1218
HindIII AAGCTT 3 cut(s) 716, 795, 1151
HinfI GANTC 4 cut(s) 671, 881, 923, 1184
HpaI GTTAAC 1 cut(s) 1218
HpaII CCGG 2 cut(s) 492, 506
HphI GGTGA 1 cut(s) 495
Hpy166II GTNNAC 4 cut(s) 342, 532, 1005, 1218
Hpy188I TCNGA 9 cut(s) 62, 150, 484, 514, 544, 723, 852, 897, 1250
Hpy188III TCNNGA 6 cut(s) 554, 560, 679, 688, 764, 1371
Hpy8I GTNNAC 4 cut(s) 342, 532, 1005, 1218
Hpy99I CGWCG 3 cut(s) 371, 376, 1142
HpyAV CCTTC 8 cut(s) 77, 185, 293, 907, 1030, 1036, 1168, 1321
HpyCH4III ACNGT 5 cut(s) 17, 146, 451, 1350, 1434
HpyCH4IV ACGT 2 cut(s) 359, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 112, 114, 437
HpyF3I CTNAG 5 cut(s) 86, 111, 980, 1025, 1369
HpySE526I ACGT 2 cut(s) 359, 500
Hsp92II CATG 4 cut(s) 40, 253, 259, 299
HspAI GCGC 3 cut(s) 106, 115, 605
KspAI GTTAAC 1 cut(s) 1218
Kzo9I GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
LmnI GCTCC 5 cut(s) 23, 171, 733, 1018, 1373
Lsp1109I GCAGC 3 cut(s) 102, 142, 425
LweI GCATC 4 cut(s) 53, 102, 293, 382
MaeI CTAG 2 cut(s) 210, 221
MaeII ACGT 2 cut(s) 359, 500
MaeIII GTNAC 2 cut(s) 124, 501
MalI GATC 7 cut(s) 59, 558, 804, 955, 985, 1117, 1252
MboI GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
MboII GAAGA 8 cut(s) 41, 575, 612, 740, 758, 1043, 1348, 1411
MflI RGATCY 3 cut(s) 983, 1115, 1250
MhlI GDGCHC 4 cut(s) 28, 344, 365, 1007
MluCI AATT 9 cut(s) 515, 632, 749, 768, 933, 1071, 1203, 1265, 1322
MluI ACGCGT 1 cut(s) 369
MlyI GAGTC 2 cut(s) 890, 932
MmeI TCCRAC 1 cut(s) 830
Mph1103I ATGCAT 1 cut(s) 878
MseI TTAA 5 cut(s) 888, 992, 1055, 1217, 1313
MslI CAYNNNNRTG 4 cut(s) 254, 294, 524, 527
MspI CCGG 2 cut(s) 492, 506
MspR9I CCNGG 3 cut(s) 170, 959, 1286
MvaI CCWGG 3 cut(s) 170, 959, 1286
MvnI CGCG 3 cut(s) 355, 371, 952
MwoI GCNNNNNNNGC 3 cut(s) 112, 114, 437
NdeII GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
NlaIII CATG 4 cut(s) 40, 253, 259, 299
NlaIV GGNNCC 2 cut(s) 333, 785
NmuCI GTSAC 2 cut(s) 124, 501
NsiI ATGCAT 1 cut(s) 878
NspI RCATGY 1 cut(s) 259
OliI CACNNNNGTG 1 cut(s) 527
PciI ACATGT 1 cut(s) 255
PfeI GAWTC 2 cut(s) 671, 1184
PflFI GACNNNGTC 1 cut(s) 536
PinAI ACCGGT 1 cut(s) 505
PkrI GCNGC 3 cut(s) 92, 132, 415
Ple19I CGATCG 1 cut(s) 60
PleI GAGTC 2 cut(s) 889, 931
PpsI GAGTC 2 cut(s) 889, 931
PpuMI RGGWCCY 1 cut(s) 855
PscI ACATGT 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 855
Psp6I CCWGG 3 cut(s) 168, 957, 1284
PspGI CCWGG 3 cut(s) 168, 957, 1284
PspN4I GGNNCC 2 cut(s) 333, 785
PspPI GGNCC 3 cut(s) 172, 332, 855
PspPPI RGGWCCY 1 cut(s) 855
PstNI CAGNNNCTG 2 cut(s) 10, 118
PsuI RGATCY 3 cut(s) 983, 1115, 1250
PsyI GACNNNGTC 1 cut(s) 536
PvuI CGATCG 1 cut(s) 60
RsaI GTAC 7 cut(s) 14, 260, 461, 578, 696, 841, 1145
RsaNI GTAC 7 cut(s) 13, 259, 460, 577, 695, 840, 1144
RseI CAYNNNNRTG 4 cut(s) 254, 294, 524, 527
SaqAI TTAA 5 cut(s) 888, 992, 1055, 1217, 1313
SatI GCNGC 3 cut(s) 91, 131, 414
Sau3AI GATC 7 cut(s) 57, 556, 802, 953, 983, 1115, 1250
Sau96I GGNCC 3 cut(s) 172, 332, 855
SchI GAGTC 2 cut(s) 890, 932
ScrFI CCNGG 3 cut(s) 170, 959, 1286
SduI GDGCHC 4 cut(s) 28, 344, 365, 1007
SfaNI GCATC 4 cut(s) 53, 102, 293, 382
SgrAI CRCCGGYG 1 cut(s) 505
SinI GGWCC 1 cut(s) 855
SmiMI CAYNNNNRTG 4 cut(s) 254, 294, 524, 527
SmlI CTYRAG 2 cut(s) 181, 655
SmoI CTYRAG 2 cut(s) 181, 655
Sse9I AATT 9 cut(s) 515, 632, 749, 768, 933, 1071, 1203, 1265, 1322
SsiI CCGC 5 cut(s) 31, 44, 71, 355, 1254
SspMI CTAG 2 cut(s) 210, 221
StyD4I CCNGG 3 cut(s) 168, 957, 1284
TaaI ACNGT 5 cut(s) 17, 146, 451, 1350, 1434
TaiI ACGT 2 cut(s) 362, 503
TaqI TCGA 2 cut(s) 374, 1140
TaqII GACCGA 1 cut(s) 468
TasI AATT 9 cut(s) 515, 632, 749, 768, 933, 1071, 1203, 1265, 1322
TatI WGTACW 4 cut(s) 12, 258, 576, 694
TfiI GAWTC 2 cut(s) 671, 1184
Tru1I TTAA 5 cut(s) 888, 992, 1055, 1217, 1313
Tru9I TTAA 5 cut(s) 888, 992, 1055, 1217, 1313
TscAI CASTG 2 cut(s) 90, 204
TseFI GTSAC 2 cut(s) 124, 501
TseI GCWGC 3 cut(s) 90, 130, 413
Tsp45I GTSAC 2 cut(s) 124, 501
TspGWI ACGGA 1 cut(s) 94
TspRI CASTG 2 cut(s) 90, 204
Tth111I GACNNNGTC 1 cut(s) 536
VneI GTGCAC 2 cut(s) 340, 1003
VpaK11BI GGWCC 1 cut(s) 855
XapI RAATTY 3 cut(s) 515, 1203, 1265
XceI RCATGY 1 cut(s) 259
XspI CTAG 2 cut(s) 210, 221
Zsp2I ATGCAT 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.