Rmu_sc0010368.1_g000005

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010368.1
OG0002539
Physical Location & Seq
Forward (+)
12469 .. 14261
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010368.1_g000005.1.cds

Sequence Viewer

Length: 903 bp
atggaaaacaatgctcacttgttttgtaagtgtccaactgcttttcaaattcgttcaagtccatctttttctttacaacatactattctacctaagctgcaattcaaggagtggatgcttgagcaagcccttaatctcaaacctgaattgtttgagaaacttcttatgatcatttggggcttgtggaagaataggaataagatgctttgggagagcaggtttcaatcctcaaatgttatcatgcttggcaaccttacatggcttgatgaattccaaagagctaggaaaagttataagttaccggtccaaagaggtgcaaagaaatggcatgcgtctacaacttctaagctcaacgttgatggagcttttataccacaagaatcacatggtggaactggaggagtgtttcggagctcttgtgggactttccaagctgccttcacacaaccagtctattatgtcaactcggcacatcaaggtagtccaccaagaattgctccaggcccatcactcaggcacagaccagtccaccatctcctcccctcagccgtcgtacatcacagtccgtcgaccagtttctggaagacccacatcaccgaccagcgctacctcgatccgccctcgccacgccagcatccccgccatccaagccggcccgagctacgccacctcgacatgtatcaacacataaacacgattcggatccaggccttgaaactccacaccatcaatcaaatccatggcatcaccgatcccagaaaccatcaatcctctgaccgaatcctaggggcattagatctcccgtctggcagacaacctcatgacggcagagaggccggaagccagccccgacatccgggctctgcgggacaagaagaagatttcaaacggcgtagaccttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

300

Amino Acids

34.41

Weight (kDa)

10.25

Isoelectric Point (pI)

61.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 207
AccB7I CCANNNNNTGG 1 cut(s) 579
AccI GTMKAC 3 cut(s) 335, 569, 895
AciI CCGC 3 cut(s) 617, 640, 866
AclI AACGTT 1 cut(s) 354
AclWI GGATC 4 cut(s) 608, 697, 710, 746
AcsI RAATTY 2 cut(s) 48, 269
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 1 cut(s) 555
AfeI AGCGCT 1 cut(s) 605
AfiI CCNNNNNNNGG 3 cut(s) 579, 824, 856
AflIII ACRYGT 1 cut(s) 675
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 6 cut(s) 47, 57, 106, 224, 715, 886
AjnI CCWGG 2 cut(s) 499, 705
AluBI AGCT 7 cut(s) 97, 281, 349, 365, 414, 434, 661
AluI AGCT 7 cut(s) 97, 281, 349, 365, 414, 434, 661
Alw21I GWGCWC 1 cut(s) 416
AlwI GGATC 4 cut(s) 608, 697, 710, 746
AlwNI CAGNNNCTG 1 cut(s) 579
Ama87I CYCGRG 1 cut(s) 656
Aor51HI AGCGCT 1 cut(s) 605
AoxI GGCC 4 cut(s) 502, 653, 708, 834
ApeKI GCWGC 2 cut(s) 97, 434
ApoI RAATTY 2 cut(s) 48, 269
AsiGI ACCGGT 1 cut(s) 301
AspA2I CCTAGG 1 cut(s) 784
AspLEI GCGC 1 cut(s) 606
AspS9I GGNCC 3 cut(s) 304, 503, 654
AsuC2I CCSGG 1 cut(s) 858
AsuHPI GGTGA 2 cut(s) 586, 739
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 1 cut(s) 304
AvrII CCTAGG 1 cut(s) 784
BamHI GGATCC 1 cut(s) 702
BanII GRGCYC 2 cut(s) 416, 863
BbsI GAAGAC 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 416
BbvCI CCTCAGC 1 cut(s) 544
BbvI GCAGC 2 cut(s) 84, 421
BccI CCATC 7 cut(s) 70, 353, 514, 540, 651, 734, 771
BceAI ACGGC 2 cut(s) 533, 841
BciT130I CCWGG 2 cut(s) 501, 707
BclI TGATCA 1 cut(s) 168
BcnI CCSGG 1 cut(s) 858
BfaI CTAG 2 cut(s) 282, 785
BfoI RGCGCY 1 cut(s) 607
BfuAI ACCTGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 796
BisI GCNGC 2 cut(s) 98, 435
BlnI CCTAGG 1 cut(s) 784
BlsI GCNGC 2 cut(s) 99, 436
Bme1390I CCNGG 3 cut(s) 501, 707, 858
Bme18I GGWCC 1 cut(s) 304
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 3 cut(s) 304, 503, 654
BmiI GGNNCC 1 cut(s) 704
BmrFI CCNGG 3 cut(s) 501, 707, 858
BmsI GCATC 4 cut(s) 105, 192, 643, 753
BpiI GAAGAC 1 cut(s) 590
BpmI CTGGAG 2 cut(s) 417, 483
Bpu10I CCTNAGC 2 cut(s) 93, 544
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 858
BsaBI GATNNNNATC 1 cut(s) 701
BsaJI CCNNGG 2 cut(s) 739, 784
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 4 cut(s) 203, 233, 403, 433
Bsc4I CCNNNNNNNGG 3 cut(s) 579, 824, 856
Bse118I RCCGGY 2 cut(s) 301, 651
Bse1I ACTGG 4 cut(s) 400, 449, 524, 573
Bse8I GATNNNNATC 1 cut(s) 701
BseBI CCWGG 2 cut(s) 501, 707
BseDI CCNNGG 2 cut(s) 739, 784
BseGI GGATG 4 cut(s) 120, 634, 643, 853
BseJI GATNNNNATC 1 cut(s) 701
BseLI CCNNNNNNNGG 3 cut(s) 579, 824, 856
BseMII CTCAG 2 cut(s) 526, 558
BseNI ACTGG 4 cut(s) 400, 449, 524, 573
BseRI GAGGAG 2 cut(s) 414, 527
BseXI GCAGC 2 cut(s) 84, 421
BshFI GGCC 4 cut(s) 504, 655, 710, 836
BshTI ACCGGT 1 cut(s) 301
BsiHKAI GWGCWC 1 cut(s) 416
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 4 cut(s) 302, 652, 837, 857
BslFI GGGAC 2 cut(s) 436, 882
BslI CCNNNNNNNGG 3 cut(s) 579, 824, 856
BsmFI GGGAC 2 cut(s) 436, 882
BsnI GGCC 4 cut(s) 504, 655, 710, 836
BsoBI CYCGRG 1 cut(s) 656
Bsp1286I GDGCHC 2 cut(s) 416, 863
Bsp143I GATC 5 cut(s) 168, 613, 702, 751, 796
Bsp19I CCATGG 1 cut(s) 739
BspACI CCGC 3 cut(s) 617, 640, 866
BspANI GGCC 4 cut(s) 504, 655, 710, 836
BspCNI CTCAG 2 cut(s) 525, 557
BspHI TCATGA 1 cut(s) 820
BspLI GGNNCC 1 cut(s) 704
BspMI ACCTGC 1 cut(s) 207
BspPI GGATC 4 cut(s) 608, 697, 710, 746
BsrFI RCCGGY 2 cut(s) 301, 651
BsrI ACTGG 4 cut(s) 400, 449, 524, 573
BssAI RCCGGY 2 cut(s) 301, 651
BssECI CCNNGG 2 cut(s) 739, 784
BssMI GATC 5 cut(s) 168, 613, 702, 751, 796
BssT1I CCWWGG 2 cut(s) 739, 784
Bst2UI CCWGG 2 cut(s) 501, 707
Bst4CI ACNGT 1 cut(s) 563
BstC8I GCNNGC 5 cut(s) 126, 330, 632, 653, 845
BstDEI CTNAG 5 cut(s) 93, 345, 512, 544, 900
BstDSI CCRYGG 1 cut(s) 739
BstF5I GGATG 4 cut(s) 120, 634, 643, 853
BstH2I RGCGCY 1 cut(s) 607
BstHHI GCGC 1 cut(s) 606
BstKTI GATC 5 cut(s) 171, 616, 705, 754, 799
BstMBI GATC 5 cut(s) 168, 613, 702, 751, 796
BstMWI GCNNNNNNNGC 2 cut(s) 631, 648
BstNI CCWGG 2 cut(s) 501, 707
BstNSI RCATGY 2 cut(s) 332, 679
BstSCI CCNGG 3 cut(s) 499, 705, 856
BstV1I GCAGC 2 cut(s) 84, 421
BstV2I GAAGAC 1 cut(s) 590
BstX2I RGATCY 2 cut(s) 702, 796
BstYI RGATCY 2 cut(s) 702, 796
BsuRI GGCC 4 cut(s) 504, 655, 710, 836
BtgI CCRYGG 1 cut(s) 739
BtsCI GGATG 4 cut(s) 120, 634, 643, 853
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 5 cut(s) 126, 330, 632, 653, 845
CaiI CAGNNNCTG 1 cut(s) 579
CciI TCATGA 1 cut(s) 820
CfoI GCGC 1 cut(s) 606
Cfr10I RCCGGY 2 cut(s) 301, 651
Cfr13I GGNCC 3 cut(s) 304, 503, 654
CseI GACGC 1 cut(s) 321
Csp6I GTAC 1 cut(s) 554
CspAI ACCGGT 1 cut(s) 301
CviAII CATG 7 cut(s) 241, 258, 329, 386, 676, 740, 821
CviQI GTAC 1 cut(s) 554
DdeI CTNAG 5 cut(s) 93, 345, 512, 544, 900
DpnI GATC 5 cut(s) 170, 615, 704, 753, 798
DpnII GATC 5 cut(s) 168, 613, 702, 751, 796
DraIII CACNNNGTG 1 cut(s) 389
EciI GGCGGA 1 cut(s) 606
Ecl136II GAGCTC 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 739, 784
Eco147I AGGCCT 1 cut(s) 710
Eco24I GRGCYC 2 cut(s) 416, 863
Eco47I GGWCC 1 cut(s) 304
Eco47III AGCGCT 1 cut(s) 605
Eco53kI GAGCTC 1 cut(s) 414
Eco88I CYCGRG 1 cut(s) 656
EcoICRI GAGCTC 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 2 cut(s) 499, 705
EcoT14I CCWWGG 2 cut(s) 739, 784
EcoT38I GRGCYC 2 cut(s) 416, 863
ErhI CCWWGG 2 cut(s) 739, 784
FaeI CATG 7 cut(s) 244, 261, 332, 389, 679, 743, 824
FalI AAGNNNNNCTT 2 cut(s) 49, 81
FaqI GGGAC 2 cut(s) 436, 882
FatI CATG 7 cut(s) 240, 257, 328, 385, 675, 739, 820
FauI CCCGC 2 cut(s) 647, 859
FbaI TGATCA 1 cut(s) 168
FblI GTMKAC 3 cut(s) 335, 569, 895
Fnu4HI GCNGC 2 cut(s) 98, 435
FokI GGATG 4 cut(s) 127, 621, 630, 840
FriOI GRGCYC 2 cut(s) 416, 863
Fsp4HI GCNGC 2 cut(s) 98, 435
FspBI CTAG 2 cut(s) 282, 785
GlaI GCGC 1 cut(s) 605
GluI GCNGC 2 cut(s) 98, 435
GsuI CTGGAG 2 cut(s) 417, 483
HaeII RGCGCY 1 cut(s) 607
HaeIII GGCC 4 cut(s) 504, 655, 710, 836
HapII CCGG 4 cut(s) 302, 652, 837, 857
HgaI GACGC 1 cut(s) 321
HhaI GCGC 1 cut(s) 606
Hin1II CATG 7 cut(s) 244, 261, 332, 389, 679, 743, 824
Hin6I GCGC 1 cut(s) 604
HinP1I GCGC 1 cut(s) 604
HincII GTYRAC 2 cut(s) 463, 570
HindII GTYRAC 2 cut(s) 463, 570
HinfI GANTC 3 cut(s) 380, 697, 780
HpaII CCGG 4 cut(s) 302, 652, 837, 857
HphI GGTGA 2 cut(s) 586, 739
Hpy166II GTNNAC 6 cut(s) 336, 463, 485, 529, 570, 896
Hpy188I TCNGA 3 cut(s) 411, 702, 775
Hpy188III TCNNGA 2 cut(s) 580, 821
Hpy8I GTNNAC 6 cut(s) 336, 463, 485, 529, 570, 896
Hpy99I CGWCG 2 cut(s) 554, 571
HpyAV CCTTC 1 cut(s) 448
HpyCH4III ACNGT 1 cut(s) 563
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 2 cut(s) 100, 317
HpyF10VI GCNNNNNNNGC 2 cut(s) 631, 648
HpyF3I CTNAG 5 cut(s) 93, 345, 512, 544, 900
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 7 cut(s) 244, 261, 332, 389, 679, 743, 824
HspAI GCGC 1 cut(s) 604
KroI GCCGGC 1 cut(s) 651
KroNI GCCGGC 1 cut(s) 653
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 5 cut(s) 168, 613, 702, 751, 796
LmnI GCTCC 3 cut(s) 362, 411, 502
Lsp1109I GCAGC 2 cut(s) 84, 421
LweI GCATC 4 cut(s) 105, 192, 643, 753
MaeI CTAG 2 cut(s) 282, 785
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 1 cut(s) 297
MalI GATC 5 cut(s) 170, 615, 704, 753, 798
MboI GATC 5 cut(s) 168, 613, 702, 751, 796
MboII GAAGA 4 cut(s) 199, 595, 887, 890
MflI RGATCY 2 cut(s) 702, 796
MhlI GDGCHC 2 cut(s) 416, 863
MluCI AATT 5 cut(s) 48, 101, 146, 269, 492
MmeI TCCRAC 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 651
MseI TTAA 1 cut(s) 132
MspI CCGG 4 cut(s) 302, 652, 837, 857
MspR9I CCNGG 3 cut(s) 501, 707, 858
MvaI CCWGG 2 cut(s) 501, 707
MwoI GCNNNNNNNGC 2 cut(s) 631, 648
NaeI GCCGGC 1 cut(s) 653
NciI CCSGG 1 cut(s) 858
NcoI CCATGG 1 cut(s) 739
NdeII GATC 5 cut(s) 168, 613, 702, 751, 796
NgoMIV GCCGGC 1 cut(s) 651
NlaIII CATG 7 cut(s) 244, 261, 332, 389, 679, 743, 824
NlaIV GGNNCC 1 cut(s) 704
NmeAIII GCCGAG 1 cut(s) 446
NspI RCATGY 2 cut(s) 332, 679
PaeI GCATGC 1 cut(s) 332
PagI TCATGA 1 cut(s) 820
PceI AGGCCT 1 cut(s) 710
PciI ACATGT 1 cut(s) 675
PdiI GCCGGC 1 cut(s) 653
PfeI GAWTC 3 cut(s) 380, 697, 780
PflMI CCANNNNNTGG 1 cut(s) 579
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 2 cut(s) 99, 436
PscI ACATGT 1 cut(s) 675
PsiI TTATAA 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 416
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 499, 705
PspGI CCWGG 2 cut(s) 499, 705
PspN4I GGNNCC 1 cut(s) 704
PspPI GGNCC 3 cut(s) 304, 503, 654
PstNI CAGNNNCTG 1 cut(s) 579
PsuI RGATCY 2 cut(s) 702, 796
RsaI GTAC 1 cut(s) 555
RsaNI GTAC 1 cut(s) 554
SacI GAGCTC 1 cut(s) 416
SalI GTCGAC 1 cut(s) 568
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 2 cut(s) 98, 435
Sau3AI GATC 5 cut(s) 168, 613, 702, 751, 796
Sau96I GGNCC 3 cut(s) 304, 503, 654
ScrFI CCNGG 3 cut(s) 501, 707, 858
SduI GDGCHC 2 cut(s) 416, 863
SfaNI GCATC 4 cut(s) 105, 192, 643, 753
SinI GGWCC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SphI GCATGC 1 cut(s) 332
Sse9I AATT 5 cut(s) 48, 101, 146, 269, 492
SseBI AGGCCT 1 cut(s) 710
SsiI CCGC 3 cut(s) 617, 640, 866
SspMI CTAG 2 cut(s) 282, 785
SstI GAGCTC 1 cut(s) 416
StuI AGGCCT 1 cut(s) 710
StyD4I CCNGG 3 cut(s) 499, 705, 856
StyI CCWWGG 2 cut(s) 739, 784
TaaI ACNGT 1 cut(s) 563
TaiI ACGT 1 cut(s) 357
TaqI TCGA 3 cut(s) 569, 612, 672
TaqII GACCGA 1 cut(s) 792
TasI AATT 5 cut(s) 48, 101, 146, 269, 492
TfiI GAWTC 3 cut(s) 380, 697, 780
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TseI GCWGC 2 cut(s) 97, 434
TspDTI ATGAA 1 cut(s) 282
TspGWI ACGGA 1 cut(s) 555
Van91I CCANNNNNTGG 1 cut(s) 579
VpaK11BI GGWCC 1 cut(s) 304
XapI RAATTY 2 cut(s) 48, 269
XceI RCATGY 2 cut(s) 332, 679
XmaJI CCTAGG 1 cut(s) 784
XmiI GTMKAC 3 cut(s) 335, 569, 895
XspI CTAG 2 cut(s) 282, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.