Rorug05G0266300

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000452
Physical Location & Seq
Reverse (-)
30039301 .. 30047258
7958 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0266300.1

Sequence Viewer

Length: 1440 bp
ATGCTTTCCTCCGGCAAGATCAAGTCGGTCGATTTCTACAGGAAAATTCCAAGAGATTTGACGGAAGCATCATTATCAGGTGCAGGATTGTCTATAGTAGCAGCTCTTGCCATGATGTTTTTATTTGGAATGGAACTGAATAATTATTTGACCTTCAGCACCTCTACATCTGTTATTGTTGACAGAAGTACTGATGGGGATTTCTTACGCATTGAGTTTAATATCAGTTTTCCTGCACTATCATGTGAATTTGCATCCCTTGACGTCAGTGATGTGTTGGGAACAAACAGATTGAATATAACAAAAACCATTCGCAAGTTCTCTATAGGTCCAGATCTGAGACCCACCGGCTCTGAGTTTCAGTCTGGTCCTGTTTTACATAACATTAAGCATGGGGATGAAGGTGATGAGTATTCTGGTGATGGTTCTGTTTCACTAACTGCTCATAATTTTGAAAGATTTACTCAACATCATCCTATTCTGGTTGTCAATTTTCATGCTCCCTGGTGCTACTGGAGCAATCGCCTGAAACCTTCATGGGAGAAGGCTGCCAAAATAATGCGAGAAAGATATGATCCAGAAATAGATGGGCGTATTCTTTTGGCAAAGGTGGATTGCACTGAAGAAGGTGACTTGTGTAGGAGGAATCATATTCAAGGATATCCATCGATTCGTATTTTTCGAAAAGGAAGTGATCTAAGGGAGGACCATGGGCACCATGAGCATGAATCTTATTACGGAGATCGAGATACAGATAGCTTGGTCAAGACAATGGAAGCTTTGGTTGCACCTATCCCCTTGGAGTCTCAAAAGCTGGCGTTAGGTGGTAACAATGAGACTGAGAATGCAAAAAGACCAGCACCATTGACAGGAGGATGTAGAATTGAAGGATATGTGCGTGTAAAGAAGGTTCCTGGAAGCCTTATCATTTCAGCTCATTCAGGAACTCATTCCTTTGATGCCTCTCAAATGAACATGTCTCACTACATATCACATCTTTCATTCGGTAGGACAATTGCTCCTAAGGTGATGAGTGATGTGAAGCGATTGATACCTTATCTTGGTCGAAGCCATGAAAGGTTGAATGGACGATCTTTTATAAATCACCGTGATATAGGAGCTAATGTCACAGTAAAGCATTATCTTCAGATAGTTAAAACGGAAGTCATAACAGGAAGATCTCACAAATTAATCGAGGAATATGAGTACACTGCCCACAGCAGTTTGATAGAGAGTCTGAACATCCCTGTTGCAAAATTCCATTTTGAGCTCTCCCCCATGCAGGTTTTAGTAACAGAAAATGAGAAATCCTTTTCGCATTTTATCACAAATGTGTGCGCTATTATTGGAGGCGTTTTCACGGTAGCAGGAATATTGGATTCAATTCTGCACAACACCCTTAGGCTGGTTAAAAAGATTGAACTAGGCAAAAACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

479

Amino Acids

53.66

Weight (kDa)

7.38

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERGIC_N PF13850 7 - 97 1.5e-22 Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)
Thioredoxin PF00085 144 - 250 2.7e-16 Thioredoxin
COPIIcoated_ERV PF07970 282 - 460 3.4e-28 Endoplasmic reticulum vesicle transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000452)

Species Orthologous Gene IDs
rosa_rugosa Rorug01G0066100 Rorug01G0066100 Rorug01G0069900 Rorug01G0089700 Rorug01G0090100 Rorug01G0097600 Rorug01G0099600 Rorug01G0101900 Rorug01G0102400 Rorug01G0118300 Rorug01G0124800 Rorug01G0169500 Rorug01G0238100 Rorug01G0451600 Rorug02G0188700 Rorug02G0229300 Rorug02G0253000 Rorug02G0256200 Rorug02G0258900 Rorug02G0339200 Rorug02G0392100 Rorug02G0491500 Rorug03G0141200 Rorug03G0192500 Rorug03G0200300 Rorug03G0218600 Rorug03G0269500 Rorug03G0269700 Rorug03G0272400 Rorug03G0276400 Rorug03G0283800 Rorug03G0334100 Rorug03G0356200 Rorug04G0002200 Rorug04G0008300 Rorug04G0031200 Rorug04G0040800 Rorug04G0063000 Rorug04G0069500.1 Rorug04G0070200 Rorug04G0114400 Rorug04G0181100 Rorug04G0205700 Rorug05G0004000 Rorug05G0018000 Rorug05G0018100 Rorug05G0090500 Rorug05G0108800 Rorug05G0118900 Rorug05G0202300 Rorug05G0214200 Rorug05G0243800 Rorug05G0243900 Rorug05G0266300 Rorug05G0272500 Rorug05G0276400 Rorug05G0291500 Rorug05G0293800 Rorug05G0350400 Rorug05G0360900 Rorug05G0369500 Rorug05G0370100 Rorug05G0370500 Rorug05G0407800 Rorug05G0418900 Rorug05G0524200 Rorug05G0526800 Rorug05G0547200 Rorug06G0004100 Rorug06G0015000 Rorug06G0027300 Rorug06G0048200 Rorug06G0059100 Rorug06G0066200 Rorug06G0128100 Rorug06G0363800.1 Rorug07G0031500 Rorug07G0184700 Rorug07G0185600 Rorug07G0189600 Rorug07G0196500 Rorug07G0200700 Rorug07G0219700 Rorug07G0219900 Rorug07G0219900 Rorug07G0223000 Rorug07G0223000 Rorug07G0230800 Rorug07G0243500 Rorug07G0244700 Rorug07G0245700 Rorug07G0279800 Rorug07G0305800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1100
AatII GACGTC 1 cut(s) 267
Acc36I ACCTGC 1 cut(s) 1273
AccB1I GGYRCC 1 cut(s) 714
AclWI GGATC 1 cut(s) 569
AcsI RAATTY 3 cut(s) 45, 248, 1256
AcuI CTGAAG 3 cut(s) 139, 642, 1130
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 190, 1208
AfiI CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 7 cut(s) 295, 455, 656, 887, 1084, 1383, 1421
AjnI CCWGG 2 cut(s) 503, 913
AleI CACNNNNGTG 1 cut(s) 1331
AluBI AGCT 7 cut(s) 104, 759, 779, 814, 935, 1121, 1270
AluI AGCT 7 cut(s) 104, 759, 779, 814, 935, 1121, 1270
Alw21I GWGCWC 1 cut(s) 1272
Alw26I GTCTC 4 cut(s) 334, 810, 830, 984
AlwI GGATC 1 cut(s) 569
ApeKI GCWGC 2 cut(s) 101, 548
ApoI RAATTY 3 cut(s) 45, 248, 1256
AseI ATTAAT 1 cut(s) 1190
Asp700I GAANNNNTTC 1 cut(s) 949
AspLEI GCGC 1 cut(s) 1340
AspS9I GGNCC 3 cut(s) 329, 368, 706
AsuHPI GGTGA 5 cut(s) 416, 431, 641, 1039, 1097
AsuII TTCGAA 1 cut(s) 682
AvaII GGWCC 3 cut(s) 329, 368, 706
AxyI CCTNAGG 2 cut(s) 1023, 1400
BaeGI GKGCMC 1 cut(s) 717
BanI GGYRCC 1 cut(s) 714
BanII GRGCYC 1 cut(s) 1272
Bbv12I GWGCWC 1 cut(s) 1272
BbvI GCAGC 2 cut(s) 113, 535
BccI CCATC 4 cut(s) 188, 416, 581, 673
BciT130I CCWGG 2 cut(s) 505, 915
BcoDI GTCTC 4 cut(s) 334, 810, 830, 984
BfaI CTAG 1 cut(s) 1424
BfmI CTRYAG 3 cut(s) 37, 93, 324
BfuAI ACCTGC 1 cut(s) 1273
BglII AGATCT 2 cut(s) 334, 1178
BisI GCNGC 2 cut(s) 102, 549
BlsI GCNGC 2 cut(s) 103, 550
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 505, 915
Bme18I GGWCC 3 cut(s) 329, 368, 706
BmgT120I GGNCC 3 cut(s) 329, 368, 706
BmiI GGNNCC 2 cut(s) 716, 912
BmrFI CCNGG 2 cut(s) 505, 915
BmsI GCATC 3 cut(s) 77, 263, 949
BpmI CTGGAG 1 cut(s) 535
Bpu14I TTCGAA 1 cut(s) 682
Bsa29I ATCGAT 1 cut(s) 668
BsaBI GATNNNNATC 1 cut(s) 664
BsaHI GRCGYC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 3 cut(s) 503, 709, 798
BsaXI ACNNNNNCTCC 4 cut(s) 1003, 1033, 1110, 1140
Bsc4I CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 518
Bse21I CCTNAGG 2 cut(s) 1023, 1400
Bse8I GATNNNNATC 1 cut(s) 664
BseBI CCWGG 2 cut(s) 505, 915
BseCI ATCGAT 1 cut(s) 668
BseDI CCNNGG 3 cut(s) 503, 709, 798
BseGI GGATG 5 cut(s) 254, 403, 472, 881, 1242
BseJI GATNNNNATC 1 cut(s) 664
BseLI CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
BseMII CTCAG 3 cut(s) 329, 345, 831
BseNI ACTGG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 717
BseXI GCAGC 2 cut(s) 113, 535
BsgI GTGCAG 3 cut(s) 102, 219, 1373
Bsh1285I CGRYCG 1 cut(s) 30
BshNI GGYRCC 1 cut(s) 714
BshVI ATCGAT 1 cut(s) 668
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 1272
BsiSI CCGG 2 cut(s) 12, 348
BslI CCNNNNNNNGG 4 cut(s) 869, 1061, 1282, 1405
BsmAI GTCTC 4 cut(s) 334, 810, 830, 984
BsmI GAATGC 1 cut(s) 850
Bso31I GGTCTC 1 cut(s) 334
Bsp119I TTCGAA 1 cut(s) 682
Bsp1286I GDGCHC 2 cut(s) 717, 1272
Bsp143I GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
Bsp19I CCATGG 1 cut(s) 709
BspCNI CTCAG 3 cut(s) 330, 346, 832
BspDI ATCGAT 1 cut(s) 668
BspLI GGNNCC 2 cut(s) 716, 912
BspMI ACCTGC 1 cut(s) 1273
BspPI GGATC 1 cut(s) 569
BspT104I TTCGAA 1 cut(s) 682
BspT107I GGYRCC 1 cut(s) 714
BspTNI GGTCTC 1 cut(s) 334
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 3 cut(s) 503, 709, 798
BssMI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
BssNI GRCGYC 1 cut(s) 264
BssT1I CCWWGG 2 cut(s) 709, 798
Bst2UI CCWGG 2 cut(s) 505, 915
Bst4CI ACNGT 3 cut(s) 1109, 1132, 1363
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 107
BstBI TTCGAA 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 816
BstDEI CTNAG 6 cut(s) 338, 354, 698, 840, 1023, 1400
BstDSI CCRYGG 1 cut(s) 709
BstF5I GGATG 5 cut(s) 254, 403, 472, 881, 1242
BstHHI GCGC 1 cut(s) 1340
BstKTI GATC 7 cut(s) 21, 337, 577, 697, 745, 1094, 1181
BstMAI GTCTC 4 cut(s) 334, 810, 830, 984
BstMBI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 4 cut(s) 107, 516, 721, 785
BstNI CCWGG 2 cut(s) 505, 915
BstNSI RCATGY 1 cut(s) 979
BstSCI CCNGG 2 cut(s) 503, 913
BstSFI CTRYAG 3 cut(s) 37, 93, 324
BstSLI GKGCMC 1 cut(s) 717
BstV1I GCAGC 2 cut(s) 113, 535
BstX2I RGATCY 2 cut(s) 334, 1178
BstYI RGATCY 2 cut(s) 334, 1178
Bsu15I ATCGAT 1 cut(s) 668
Bsu36I CCTNAGG 2 cut(s) 1023, 1400
BsuTUI ATCGAT 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 709
BtsCI GGATG 5 cut(s) 254, 403, 472, 881, 1242
BtsI GCAGTG 1 cut(s) 1209
BtsIMutI CAGTG 3 cut(s) 274, 618, 1209
BveI ACCTGC 1 cut(s) 1273
Cac8I GCNNGC 1 cut(s) 816
CfoI GCGC 1 cut(s) 1340
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 3 cut(s) 329, 368, 706
ClaI ATCGAT 1 cut(s) 668
Csp6I GTAC 2 cut(s) 189, 1207
CviQI GTAC 2 cut(s) 189, 1207
DdeI CTNAG 6 cut(s) 338, 354, 698, 840, 1023, 1400
DpnI GATC 7 cut(s) 20, 336, 576, 696, 744, 1093, 1180
DpnII GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
Ecl136II GAGCTC 1 cut(s) 1270
Eco130I CCWWGG 2 cut(s) 709, 798
Eco24I GRGCYC 1 cut(s) 1272
Eco31I GGTCTC 1 cut(s) 334
Eco32I GATATC 1 cut(s) 662
Eco47I GGWCC 3 cut(s) 329, 368, 706
Eco53kI GAGCTC 1 cut(s) 1270
Eco57I CTGAAG 3 cut(s) 139, 642, 1130
Eco81I CCTNAGG 2 cut(s) 1023, 1400
EcoICRI GAGCTC 1 cut(s) 1270
EcoRII CCWGG 2 cut(s) 503, 913
EcoRV GATATC 1 cut(s) 662
EcoT14I CCWWGG 2 cut(s) 709, 798
EcoT38I GRGCYC 1 cut(s) 1272
ErhI CCWWGG 2 cut(s) 709, 798
Fnu4HI GCNGC 2 cut(s) 102, 549
FokI GGATG 5 cut(s) 241, 410, 459, 888, 1229
FriOI GRGCYC 1 cut(s) 1272
Fsp4HI GCNGC 2 cut(s) 102, 549
FspBI CTAG 1 cut(s) 1424
GlaI GCGC 1 cut(s) 1339
GluI GCNGC 2 cut(s) 102, 549
GsuI CTGGAG 1 cut(s) 535
HapII CCGG 2 cut(s) 12, 348
HhaI GCGC 1 cut(s) 1340
Hin1I GRCGYC 1 cut(s) 264
Hin6I GCGC 1 cut(s) 1338
HinP1I GCGC 1 cut(s) 1338
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 6 cut(s) 646, 670, 728, 803, 1234, 1379
HpaII CCGG 2 cut(s) 12, 348
HphI GGTGA 5 cut(s) 416, 431, 641, 1039, 1097
Hpy166II GTNNAC 2 cut(s) 181, 1209
Hpy188I TCNGA 4 cut(s) 339, 355, 1149, 1239
Hpy188III TCNNGA 5 cut(s) 332, 578, 746, 766, 942
Hpy8I GTNNAC 2 cut(s) 181, 1209
HpyAV CCTTC 7 cut(s) 163, 395, 538, 543, 620, 881, 901
HpyCH4III ACNGT 3 cut(s) 1109, 1132, 1363
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 9 cut(s) 83, 236, 254, 618, 788, 848, 1253, 1282, 1390
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 516, 721, 785
HpyF3I CTNAG 6 cut(s) 338, 354, 698, 840, 1023, 1400
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 264
HspAI GCGC 1 cut(s) 1338
Kzo9I GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
LmnI GCTCC 4 cut(s) 505, 516, 1024, 1118
Lsp1109I GCAGC 2 cut(s) 113, 535
LweI GCATC 3 cut(s) 77, 263, 949
MaeI CTAG 1 cut(s) 1424
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 4 cut(s) 629, 827, 1126, 1291
MalI GATC 7 cut(s) 20, 336, 576, 696, 744, 1093, 1180
MboI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
MboII GAAGA 3 cut(s) 635, 1136, 1188
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 2 cut(s) 334, 1178
MhlI GDGCHC 2 cut(s) 717, 1272
MlyI GAGTC 2 cut(s) 812, 1243
MnlI CCTC 8 cut(s) 19, 172, 636, 697, 866, 973, 1189, 1343
MroXI GAANNNNTTC 1 cut(s) 949
MseI TTAA 5 cut(s) 219, 387, 1155, 1190, 1410
MslI CAYNNNNRTG 4 cut(s) 241, 396, 723, 1331
MspI CCGG 2 cut(s) 12, 348
MspR9I CCNGG 2 cut(s) 505, 915
MunI CAATTG 1 cut(s) 1014
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 2 cut(s) 505, 915
MwoI GCNNNNNNNGC 4 cut(s) 107, 516, 721, 785
NcoI CCATGG 1 cut(s) 709
NdeII GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
NlaIV GGNNCC 2 cut(s) 716, 912
NmuCI GTSAC 2 cut(s) 629, 1126
NspI RCATGY 1 cut(s) 979
NspV TTCGAA 1 cut(s) 682
OliI CACNNNNGTG 1 cut(s) 1331
PciI ACATGT 1 cut(s) 975
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PctI GAATGC 1 cut(s) 850
PdmI GAANNNNTTC 1 cut(s) 949
PfeI GAWTC 4 cut(s) 646, 670, 728, 1379
PfoI TCCNGGA 1 cut(s) 913
PkrI GCNGC 2 cut(s) 103, 550
PleI GAGTC 2 cut(s) 811, 1242
PpsI GAGTC 2 cut(s) 811, 1242
PscI ACATGT 1 cut(s) 975
PshBI ATTAAT 1 cut(s) 1190
PsiI TTATAA 1 cut(s) 1100
Psp124BI GAGCTC 1 cut(s) 1272
Psp6I CCWGG 2 cut(s) 503, 913
PspGI CCWGG 2 cut(s) 503, 913
PspN4I GGNNCC 2 cut(s) 716, 912
PspPI GGNCC 3 cut(s) 329, 368, 706
PsrI GAACNNNNNNTAC 2 cut(s) 894, 926
PsuI RGATCY 2 cut(s) 334, 1178
RsaI GTAC 2 cut(s) 190, 1208
RsaNI GTAC 2 cut(s) 189, 1207
RseI CAYNNNNRTG 4 cut(s) 241, 396, 723, 1331
SacI GAGCTC 1 cut(s) 1272
SaqAI TTAA 5 cut(s) 219, 387, 1155, 1190, 1410
SatI GCNGC 2 cut(s) 102, 549
Sau3AI GATC 7 cut(s) 18, 334, 574, 694, 742, 1091, 1178
Sau96I GGNCC 3 cut(s) 329, 368, 706
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 2 cut(s) 812, 1243
ScrFI CCNGG 2 cut(s) 505, 915
SduI GDGCHC 2 cut(s) 717, 1272
SfaNI GCATC 3 cut(s) 77, 263, 949
SfcI CTRYAG 3 cut(s) 37, 93, 324
SfuI TTCGAA 1 cut(s) 682
SinI GGWCC 3 cut(s) 329, 368, 706
SmiMI CAYNNNNRTG 4 cut(s) 241, 396, 723, 1331
SspI AATATT 1 cut(s) 1374
SspMI CTAG 1 cut(s) 1424
SstI GAGCTC 1 cut(s) 1272
StyD4I CCNGG 2 cut(s) 503, 913
StyI CCWWGG 2 cut(s) 709, 798
TaaI ACNGT 3 cut(s) 1109, 1132, 1363
TaiI ACGT 1 cut(s) 267
TaqI TCGA 6 cut(s) 30, 668, 682, 745, 1066, 1194
TaqII GACCGA 1 cut(s) 16
TatI WGTACW 2 cut(s) 188, 1206
TfiI GAWTC 4 cut(s) 646, 670, 728, 1379
Tru1I TTAA 5 cut(s) 219, 387, 1155, 1190, 1410
Tru9I TTAA 5 cut(s) 219, 387, 1155, 1190, 1410
TscAI CASTG 3 cut(s) 274, 625, 1216
TseFI GTSAC 2 cut(s) 629, 1126
TseI GCWGC 2 cut(s) 101, 548
Tsp45I GTSAC 2 cut(s) 629, 1126
TspDTI ATGAA 7 cut(s) 414, 485, 525, 741, 986, 990, 1089
TspGWI ACGGA 3 cut(s) 77, 753, 1175
TspRI CASTG 3 cut(s) 274, 625, 1216
VpaK11BI GGWCC 3 cut(s) 329, 368, 706
VspI ATTAAT 1 cut(s) 1190
XapI RAATTY 3 cut(s) 45, 248, 1256
XceI RCATGY 1 cut(s) 979
XmnI GAANNNNTTC 1 cut(s) 949
XspI CTAG 1 cut(s) 1424
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.