Homeodomain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 3 cut(s) | 271, 592, 1210 |
| AatII | GACGTC | 1 cut(s) | 1934 |
| Acc16I | TGCGCA | 1 cut(s) | 448 |
| Acc36I | ACCTGC | 3 cut(s) | 271, 592, 1210 |
| AccB1I | GGYRCC | 1 cut(s) | 169 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 992, 1448 |
| AciI | CCGC | 1 cut(s) | 1943 |
| AclWI | GGATC | 3 cut(s) | 305, 928, 1068 |
| AcoI | YGGCCR | 2 cut(s) | 994, 1702 |
| AcsI | RAATTY | 4 cut(s) | 761, 862, 1340, 2340 |
| AcuI | CTGAAG | 3 cut(s) | 318, 1797, 2099 |
| AcyI | GRCGYC | 1 cut(s) | 1931 |
| AfaI | GTAC | 5 cut(s) | 304, 395, 1228, 2131, 2153 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 731, 944, 960, 992, 1182, 1448 |
| AjiI | CACGTC | 1 cut(s) | 2288 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 649, 681, 714, 746 |
| Alw21I | GWGCWC | 1 cut(s) | 803 |
| Alw26I | GTCTC | 8 cut(s) | 907, 1043, 1115, 1733, 1980, 2215, 2273, 2278 |
| AlwI | GGATC | 3 cut(s) | 305, 928, 1068 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 341, 1700 |
| AoxI | GGCC | 4 cut(s) | 946, 994, 1702, 1845 |
| ApeKI | GCWGC | 6 cut(s) | 245, 683, 686, 1940, 1957, 1960 |
| ApoI | RAATTY | 4 cut(s) | 761, 862, 1340, 2340 |
| AseI | ATTAAT | 2 cut(s) | 69, 1179 |
| AspLEI | GCGC | 2 cut(s) | 449, 2313 |
| AspS9I | GGNCC | 1 cut(s) | 1845 |
| AsuHPI | GGTGA | 8 cut(s) | 65, 295, 488, 684, 746, 848, 1314, 1662 |
| AxyI | CCTNAGG | 1 cut(s) | 726 |
| BalI | TGGCCA | 2 cut(s) | 996, 1704 |
| BanI | GGYRCC | 1 cut(s) | 169 |
| BanII | GRGCYC | 1 cut(s) | 803 |
| BauI | CACGAG | 1 cut(s) | 317 |
| BbsI | GAAGAC | 4 cut(s) | 19, 971, 1235, 1901 |
| Bbv12I | GWGCWC | 1 cut(s) | 803 |
| BbvI | GCAGC | 6 cut(s) | 232, 670, 673, 1927, 1947, 1969 |
| BccI | CCATC | 3 cut(s) | 398, 994, 1826 |
| BceAI | ACGGC | 1 cut(s) | 1832 |
| BclI | TGATCA | 3 cut(s) | 34, 700, 766 |
| BcoDI | GTCTC | 8 cut(s) | 907, 1043, 1115, 1733, 1980, 2215, 2273, 2278 |
| BfaI | CTAG | 5 cut(s) | 90, 855, 1842, 1871, 2318 |
| BfmI | CTRYAG | 3 cut(s) | 467, 1055, 1961 |
| BfoI | RGCGCY | 1 cut(s) | 2314 |
| BfuAI | ACCTGC | 3 cut(s) | 271, 592, 1210 |
| BisI | GCNGC | 6 cut(s) | 246, 684, 687, 1941, 1958, 1961 |
| BlsI | GCNGC | 6 cut(s) | 247, 685, 688, 1942, 1959, 1962 |
| BmgBI | CACGTC | 1 cut(s) | 2288 |
| BmgT120I | GGNCC | 1 cut(s) | 1845 |
| BmiI | GGNNCC | 3 cut(s) | 171, 723, 1809 |
| BmsI | GCATC | 8 cut(s) | 613, 829, 1618, 1681, 2119, 2201, 2239, 2286 |
| BpiI | GAAGAC | 4 cut(s) | 19, 971, 1235, 1901 |
| BpmI | CTGGAG | 5 cut(s) | 73, 1242, 1743, 1806, 2285 |
| BsaHI | GRCGYC | 1 cut(s) | 1931 |
| BsaI | GGTCTC | 1 cut(s) | 907 |
| BsaJI | CCNNGG | 2 cut(s) | 293, 2011 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 99, 129, 1937, 1967, 2283, 2313 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 731, 944, 960, 992, 1182, 1448 |
| Bse1I | ACTGG | 4 cut(s) | 1705, 1760, 1789, 2302 |
| Bse21I | CCTNAGG | 1 cut(s) | 726 |
| BseDI | CCNNGG | 2 cut(s) | 293, 2011 |
| BseGI | GGATG | 2 cut(s) | 149, 1837 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 731, 944, 960, 992, 1182, 1448 |
| BseMII | CTCAG | 7 cut(s) | 667, 717, 731, 888, 1110, 1626, 1991 |
| BseNI | ACTGG | 4 cut(s) | 1705, 1760, 1789, 2302 |
| BseRI | GAGGAG | 3 cut(s) | 1652, 1739, 2066 |
| BseXI | GCAGC | 6 cut(s) | 232, 670, 673, 1927, 1947, 1969 |
| BseYI | CCCAGC | 1 cut(s) | 76 |
| BsgI | GTGCAG | 2 cut(s) | 596, 1509 |
| BshFI | GGCC | 4 cut(s) | 948, 996, 1704, 1847 |
| BshNI | GGYRCC | 1 cut(s) | 169 |
| BsiHKAI | GWGCWC | 1 cut(s) | 803 |
| BsiSI | CCGG | 1 cut(s) | 593 |
| BslFI | GGGAC | 3 cut(s) | 550, 1745, 1881 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 731, 944, 960, 992, 1182, 1448 |
| BsmAI | GTCTC | 8 cut(s) | 907, 1043, 1115, 1733, 1980, 2215, 2273, 2278 |
| BsmBI | CGTCTC | 2 cut(s) | 2273, 2278 |
| BsmFI | GGGAC | 3 cut(s) | 550, 1745, 1881 |
| BsmI | GAATGC | 1 cut(s) | 1046 |
| BsnI | GGCC | 4 cut(s) | 948, 996, 1704, 1847 |
| Bso31I | GGTCTC | 1 cut(s) | 907 |
| Bsp1286I | GDGCHC | 1 cut(s) | 803 |
| Bsp143I | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| Bsp19I | CCATGG | 1 cut(s) | 293 |
| BspACI | CCGC | 1 cut(s) | 1943 |
| BspANI | GGCC | 4 cut(s) | 948, 996, 1704, 1847 |
| BspCNI | CTCAG | 7 cut(s) | 666, 718, 730, 889, 1111, 1627, 1990 |
| BspHI | TCATGA | 2 cut(s) | 1126, 2271 |
| BspLI | GGNNCC | 3 cut(s) | 171, 723, 1809 |
| BspMAI | CTGCAG | 1 cut(s) | 1965 |
| BspMI | ACCTGC | 3 cut(s) | 271, 592, 1210 |
| BspPI | GGATC | 3 cut(s) | 305, 928, 1068 |
| BspQI | GCTCTTC | 1 cut(s) | 351 |
| BspT107I | GGYRCC | 1 cut(s) | 169 |
| BspTNI | GGTCTC | 1 cut(s) | 907 |
| BsrI | ACTGG | 4 cut(s) | 1705, 1760, 1789, 2302 |
| BssECI | CCNNGG | 2 cut(s) | 293, 2011 |
| BssMI | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| BssNI | GRCGYC | 1 cut(s) | 1931 |
| BssSI | CACGAG | 1 cut(s) | 317 |
| BssT1I | CCWWGG | 2 cut(s) | 293, 2011 |
| Bst2BI | CACGAG | 1 cut(s) | 317 |
| Bst4CI | ACNGT | 7 cut(s) | 471, 541, 574, 784, 907, 1021, 2176 |
| Bst6I | CTCTTC | 4 cut(s) | 351, 460, 1088, 1400 |
| BstACI | GRCGYC | 1 cut(s) | 1931 |
| BstC8I | GCNNGC | 3 cut(s) | 82, 1069, 1173 |
| BstDSI | CCRYGG | 1 cut(s) | 293 |
| BstF5I | GGATG | 2 cut(s) | 149, 1837 |
| BstH2I | RGCGCY | 1 cut(s) | 2314 |
| BstHHI | GCGC | 2 cut(s) | 449, 2313 |
| BstKTI | GATC | 8 cut(s) | 37, 300, 703, 769, 936, 1076, 1644, 2103 |
| BstMAI | GTCTC | 8 cut(s) | 907, 1043, 1115, 1733, 1980, 2215, 2273, 2278 |
| BstMBI | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 63, 350, 359, 598, 1211 |
| BstNSI | RCATGY | 1 cut(s) | 1468 |
| BstSFI | CTRYAG | 3 cut(s) | 467, 1055, 1961 |
| BstV1I | GCAGC | 6 cut(s) | 232, 670, 673, 1927, 1947, 1969 |
| BstV2I | GAAGAC | 4 cut(s) | 19, 971, 1235, 1901 |
| BstX2I | RGATCY | 1 cut(s) | 1073 |
| BstYI | RGATCY | 1 cut(s) | 1073 |
| Bsu36I | CCTNAGG | 1 cut(s) | 726 |
| BsuRI | GGCC | 4 cut(s) | 948, 996, 1704, 1847 |
| BtgI | CCRYGG | 1 cut(s) | 293 |
| BtrI | CACGTC | 1 cut(s) | 2288 |
| BtsCI | GGATG | 2 cut(s) | 149, 1837 |
| BtsIMutI | CAGTG | 2 cut(s) | 903, 1325 |
| BveI | ACCTGC | 3 cut(s) | 271, 592, 1210 |
| Cac8I | GCNNGC | 3 cut(s) | 82, 1069, 1173 |
| CaiI | CAGNNNCTG | 2 cut(s) | 341, 1700 |
| CciI | TCATGA | 2 cut(s) | 1126, 2271 |
| CfoI | GCGC | 2 cut(s) | 449, 2313 |
| Cfr13I | GGNCC | 1 cut(s) | 1845 |
| Csp6I | GTAC | 5 cut(s) | 303, 394, 1227, 2130, 2152 |
| CviAII | CATG | 8 cut(s) | 294, 350, 1127, 1247, 1465, 1678, 1993, 2272 |
| CviQI | GTAC | 5 cut(s) | 303, 394, 1227, 2130, 2152 |
| DpnI | GATC | 8 cut(s) | 36, 299, 702, 768, 935, 1075, 1643, 2102 |
| DpnII | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| DraI | TTTAAA | 1 cut(s) | 747 |
| EaeI | YGGCCR | 2 cut(s) | 994, 1702 |
| Eam1104I | CTCTTC | 4 cut(s) | 351, 460, 1088, 1400 |
| EarI | CTCTTC | 4 cut(s) | 351, 460, 1088, 1400 |
| Ecl136II | GAGCTC | 1 cut(s) | 801 |
| Eco130I | CCWWGG | 2 cut(s) | 293, 2011 |
| Eco24I | GRGCYC | 1 cut(s) | 803 |
| Eco31I | GGTCTC | 1 cut(s) | 907 |
| Eco53kI | GAGCTC | 1 cut(s) | 801 |
| Eco57I | CTGAAG | 3 cut(s) | 318, 1797, 2099 |
| Eco81I | CCTNAGG | 1 cut(s) | 726 |
| EcoICRI | GAGCTC | 1 cut(s) | 801 |
| EcoRI | GAATTC | 1 cut(s) | 2340 |
| EcoT14I | CCWWGG | 2 cut(s) | 293, 2011 |
| EcoT22I | ATGCAT | 1 cut(s) | 844 |
| EcoT38I | GRGCYC | 1 cut(s) | 803 |
| ErhI | CCWWGG | 2 cut(s) | 293, 2011 |
| Esp3I | CGTCTC | 2 cut(s) | 2273, 2278 |
| FaeI | CATG | 8 cut(s) | 297, 353, 1130, 1250, 1468, 1681, 1996, 2275 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1263, 1295 |
| FaqI | GGGAC | 3 cut(s) | 550, 1745, 1881 |
| FatI | CATG | 8 cut(s) | 293, 349, 1126, 1246, 1464, 1677, 1992, 2271 |
| FbaI | TGATCA | 3 cut(s) | 34, 700, 766 |
| Fnu4HI | GCNGC | 6 cut(s) | 246, 684, 687, 1941, 1958, 1961 |
| FokI | GGATG | 2 cut(s) | 156, 1844 |
| FriOI | GRGCYC | 1 cut(s) | 803 |
| Fsp4HI | GCNGC | 6 cut(s) | 246, 684, 687, 1941, 1958, 1961 |
| FspBI | CTAG | 5 cut(s) | 90, 855, 1842, 1871, 2318 |
| FspI | TGCGCA | 1 cut(s) | 448 |
| GlaI | GCGC | 2 cut(s) | 448, 2312 |
| GluI | GCNGC | 6 cut(s) | 246, 684, 687, 1941, 1958, 1961 |
| GsaI | CCCAGC | 1 cut(s) | 80 |
| GsuI | CTGGAG | 5 cut(s) | 73, 1242, 1743, 1806, 2285 |
| HaeII | RGCGCY | 1 cut(s) | 2314 |
| HaeIII | GGCC | 4 cut(s) | 948, 996, 1704, 1847 |
| HapII | CCGG | 1 cut(s) | 593 |
| HhaI | GCGC | 2 cut(s) | 449, 2313 |
| Hin1I | GRCGYC | 1 cut(s) | 1931 |
| Hin1II | CATG | 8 cut(s) | 297, 353, 1130, 1250, 1468, 1681, 1996, 2275 |
| Hin6I | GCGC | 2 cut(s) | 447, 2311 |
| HinP1I | GCGC | 2 cut(s) | 447, 2311 |
| HindIII | AAGCTT | 5 cut(s) | 662, 1212, 1399, 2000, 2204 |
| HpaII | CCGG | 1 cut(s) | 593 |
| HphI | GGTGA | 8 cut(s) | 65, 295, 488, 684, 746, 848, 1314, 1662 |
| Hpy166II | GTNNAC | 3 cut(s) | 118, 2152, 2291 |
| Hpy8I | GTNNAC | 3 cut(s) | 118, 2152, 2291 |
| HpyAV | CCTTC | 6 cut(s) | 322, 1148, 1491, 1745, 1821, 2132 |
| HpyCH4III | ACNGT | 7 cut(s) | 471, 541, 574, 784, 907, 1021, 2176 |
| HpyCH4IV | ACGT | 2 cut(s) | 1931, 2287 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 63, 350, 359, 598, 1211 |
| HpySE526I | ACGT | 2 cut(s) | 1931, 2287 |
| Hsp92I | GRCGYC | 1 cut(s) | 1931 |
| Hsp92II | CATG | 8 cut(s) | 297, 353, 1130, 1250, 1468, 1681, 1996, 2275 |
| HspAI | GCGC | 2 cut(s) | 447, 2311 |
| Ksp22I | TGATCA | 3 cut(s) | 34, 700, 766 |
| Kzo9I | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| LguI | GCTCTTC | 1 cut(s) | 351 |
| LmnI | GCTCC | 5 cut(s) | 54, 407, 680, 2053, 2304 |
| Lsp1109I | GCAGC | 6 cut(s) | 232, 670, 673, 1927, 1947, 1969 |
| LweI | GCATC | 8 cut(s) | 613, 829, 1618, 1681, 2119, 2201, 2239, 2286 |
| MaeI | CTAG | 5 cut(s) | 90, 855, 1842, 1871, 2318 |
| MaeII | ACGT | 2 cut(s) | 1931, 2287 |
| MaeIII | GTNAC | 5 cut(s) | 238, 505, 919, 1352, 2081 |
| MalI | GATC | 8 cut(s) | 36, 299, 702, 768, 935, 1075, 1643, 2102 |
| MboI | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| MfeI | CAATTG | 3 cut(s) | 46, 102, 2229 |
| MflI | RGATCY | 1 cut(s) | 1073 |
| MhlI | GDGCHC | 1 cut(s) | 803 |
| MlsI | TGGCCA | 2 cut(s) | 996, 1704 |
| MluNI | TGGCCA | 2 cut(s) | 996, 1704 |
| MlyI | GAGTC | 4 cut(s) | 113, 878, 1117, 1982 |
| MmeI | TCCRAC | 1 cut(s) | 1466 |
| Mox20I | TGGCCA | 2 cut(s) | 996, 1704 |
| Mph1103I | ATGCAT | 1 cut(s) | 844 |
| MscI | TGGCCA | 2 cut(s) | 996, 1704 |
| MseI | TTAA | 5 cut(s) | 69, 746, 1179, 2166, 2306 |
| Msp20I | TGGCCA | 2 cut(s) | 996, 1704 |
| MspA1I | CMGCKG | 2 cut(s) | 245, 1960 |
| MspI | CCGG | 1 cut(s) | 593 |
| MunI | CAATTG | 3 cut(s) | 46, 102, 2229 |
| Mva1269I | GAATGC | 1 cut(s) | 1046 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 63, 350, 359, 598, 1211 |
| NcoI | CCATGG | 1 cut(s) | 293 |
| NdeII | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| NlaIII | CATG | 8 cut(s) | 297, 353, 1130, 1250, 1468, 1681, 1996, 2275 |
| NlaIV | GGNNCC | 3 cut(s) | 171, 723, 1809 |
| NmeAIII | GCCGAG | 1 cut(s) | 974 |
| NmuCI | GTSAC | 3 cut(s) | 919, 1352, 2081 |
| NsbI | TGCGCA | 1 cut(s) | 448 |
| NsiI | ATGCAT | 1 cut(s) | 844 |
| NspI | RCATGY | 1 cut(s) | 1468 |
| PagI | TCATGA | 2 cut(s) | 1126, 2271 |
| PaqCI | CACCTGC | 3 cut(s) | 271, 592, 1210 |
| PciSI | GCTCTTC | 1 cut(s) | 351 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1009 |
| PctI | GAATGC | 1 cut(s) | 1046 |
| PfeI | GAWTC | 8 cut(s) | 5, 463, 1061, 1452, 1649, 1697, 1981, 2262 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 992, 1448 |
| PkrI | GCNGC | 6 cut(s) | 247, 685, 688, 1942, 1959, 1962 |
| PleI | GAGTC | 4 cut(s) | 113, 878, 1117, 1981 |
| PpsI | GAGTC | 4 cut(s) | 113, 878, 1117, 1981 |
| PshBI | ATTAAT | 2 cut(s) | 69, 1179 |
| Psp124BI | GAGCTC | 1 cut(s) | 803 |
| PspFI | CCCAGC | 1 cut(s) | 76 |
| PspN4I | GGNNCC | 3 cut(s) | 171, 723, 1809 |
| PspPI | GGNCC | 1 cut(s) | 1845 |
| PstI | CTGCAG | 1 cut(s) | 1965 |
| PstNI | CAGNNNCTG | 2 cut(s) | 341, 1700 |
| PsuI | RGATCY | 1 cut(s) | 1073 |
| PvuII | CAGCTG | 2 cut(s) | 245, 1960 |
| RsaI | GTAC | 5 cut(s) | 304, 395, 1228, 2131, 2153 |
| RsaNI | GTAC | 5 cut(s) | 303, 394, 1227, 2130, 2152 |
| SacI | GAGCTC | 1 cut(s) | 803 |
| SapI | GCTCTTC | 1 cut(s) | 351 |
| SaqAI | TTAA | 5 cut(s) | 69, 746, 1179, 2166, 2306 |
| SatI | GCNGC | 6 cut(s) | 246, 684, 687, 1941, 1958, 1961 |
| Sau3AI | GATC | 8 cut(s) | 34, 297, 700, 766, 933, 1073, 1641, 2100 |
| Sau96I | GGNCC | 1 cut(s) | 1845 |
| SchI | GAGTC | 4 cut(s) | 113, 878, 1117, 1982 |
| SduI | GDGCHC | 1 cut(s) | 803 |
| SfaNI | GCATC | 8 cut(s) | 613, 829, 1618, 1681, 2119, 2201, 2239, 2286 |
| SfcI | CTRYAG | 3 cut(s) | 467, 1055, 1961 |
| SmiI | ATTTAAAT | 1 cut(s) | 747 |
| SsiI | CCGC | 1 cut(s) | 1943 |
| SspMI | CTAG | 5 cut(s) | 90, 855, 1842, 1871, 2318 |
| SstI | GAGCTC | 1 cut(s) | 803 |
| StyI | CCWWGG | 2 cut(s) | 293, 2011 |
| SwaI | ATTTAAAT | 1 cut(s) | 747 |
| TaaI | ACNGT | 7 cut(s) | 471, 541, 574, 784, 907, 1021, 2176 |
| TaiI | ACGT | 2 cut(s) | 1934, 2290 |
| TaqI | TCGA | 2 cut(s) | 461, 1580 |
| TatI | WGTACW | 2 cut(s) | 393, 2129 |
| TfiI | GAWTC | 8 cut(s) | 5, 463, 1061, 1452, 1649, 1697, 1981, 2262 |
| Tru1I | TTAA | 5 cut(s) | 69, 746, 1179, 2166, 2306 |
| Tru9I | TTAA | 5 cut(s) | 69, 746, 1179, 2166, 2306 |
| TscAI | CASTG | 2 cut(s) | 910, 1332 |
| TseFI | GTSAC | 3 cut(s) | 919, 1352, 2081 |
| TseI | GCWGC | 6 cut(s) | 245, 683, 686, 1940, 1957, 1960 |
| Tsp45I | GTSAC | 3 cut(s) | 919, 1352, 2081 |
| TspDTI | ATGAA | 8 cut(s) | 338, 674, 1235, 1281, 1676, 1839, 1907, 2288 |
| TspGWI | ACGGA | 2 cut(s) | 896, 1018 |
| TspRI | CASTG | 2 cut(s) | 910, 1332 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 992, 1448 |
| VspI | ATTAAT | 2 cut(s) | 69, 1179 |
| XapI | RAATTY | 4 cut(s) | 761, 862, 1340, 2340 |
| XceI | RCATGY | 1 cut(s) | 1468 |
| XspI | CTAG | 5 cut(s) | 90, 855, 1842, 1871, 2318 |
| ZraI | GACGTC | 1 cut(s) | 1932 |
| Zsp2I | ATGCAT | 1 cut(s) | 844 |