Homeodomain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1385 |
| AatII | GACGTC | 2 cut(s) | 1041, 1188 |
| AccBSI | CCGCTC | 1 cut(s) | 668 |
| AccI | GTMKAC | 2 cut(s) | 467, 651 |
| AccII | CGCG | 2 cut(s) | 743, 858 |
| AccIII | TCCGGA | 2 cut(s) | 247, 1061 |
| AciI | CCGC | 7 cut(s) | 570, 573, 662, 666, 856, 882, 1288 |
| AclWI | GGATC | 3 cut(s) | 1059, 1072, 1441 |
| AcoI | YGGCCR | 4 cut(s) | 156, 663, 876, 1347 |
| AcsI | RAATTY | 3 cut(s) | 692, 923, 1014 |
| AcuI | CTGAAG | 3 cut(s) | 109, 416, 1032 |
| AcvI | CACGTG | 1 cut(s) | 960 |
| AcyI | GRCGYC | 2 cut(s) | 1038, 1185 |
| AfaI | GTAC | 6 cut(s) | 56, 239, 539, 757, 1033, 1262 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 165, 247, 561, 1025, 1074 |
| AflII | CTTAAG | 1 cut(s) | 836 |
| AgsI | TTSAA | 6 cut(s) | 49, 409, 768, 928, 1012, 1091 |
| AhlI | ACTAGT | 3 cut(s) | 175, 656, 752 |
| AjiI | CACGTC | 1 cut(s) | 1213 |
| AleI | CACNNNNGTG | 1 cut(s) | 1445 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 914, 946 |
| AluBI | AGCT | 7 cut(s) | 129, 314, 436, 454, 796, 1008, 1328 |
| AluI | AGCT | 7 cut(s) | 129, 314, 436, 454, 796, 1008, 1328 |
| Alw21I | GWGCWC | 2 cut(s) | 194, 293 |
| Alw26I | GTCTC | 5 cut(s) | 854, 1003, 1076, 1103, 1289 |
| Alw44I | GTGCAC | 1 cut(s) | 289 |
| AlwI | GGATC | 3 cut(s) | 1059, 1072, 1441 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 588 |
| Ama87I | CYCGRG | 1 cut(s) | 805 |
| Aor13HI | TCCGGA | 2 cut(s) | 247, 1061 |
| AoxI | GGCC | 6 cut(s) | 156, 402, 663, 876, 966, 1347 |
| ApaLI | GTGCAC | 1 cut(s) | 289 |
| ApeKI | GCWGC | 2 cut(s) | 609, 954 |
| ApoI | RAATTY | 3 cut(s) | 692, 923, 1014 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 105, 137 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 122 |
| AspLEI | GCGC | 3 cut(s) | 614, 701, 745 |
| AspS9I | GGNCC | 3 cut(s) | 271, 1339, 1378 |
| AsuC2I | CCSGG | 2 cut(s) | 340, 866 |
| AsuHPI | GGTGA | 5 cut(s) | 163, 242, 394, 587, 862 |
| AsuII | TTCGAA | 2 cut(s) | 144, 498 |
| AvaI | CYCGRG | 1 cut(s) | 805 |
| AvaII | GGWCC | 3 cut(s) | 271, 1339, 1378 |
| AxyI | CCTNAGG | 1 cut(s) | 560 |
| BaeGI | GKGCMC | 1 cut(s) | 293 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 739, 772, 1023, 1056 |
| BalI | TGGCCA | 2 cut(s) | 158, 878 |
| BamHI | GGATCC | 1 cut(s) | 1064 |
| BanII | GRGCYC | 1 cut(s) | 1422 |
| BauI | CACGAG | 1 cut(s) | 976 |
| BbrPI | CACGTG | 1 cut(s) | 960 |
| BbsI | GAAGAC | 1 cut(s) | 115 |
| Bbv12I | GWGCWC | 2 cut(s) | 194, 293 |
| BbvCI | CCTCAGC | 1 cut(s) | 904 |
| BbvI | GCAGC | 2 cut(s) | 621, 966 |
| BccI | CCATC | 6 cut(s) | 148, 314, 512, 976, 1156, 1390 |
| BceAI | ACGGC | 1 cut(s) | 1411 |
| BcnI | CCSGG | 2 cut(s) | 340, 866 |
| BcoDI | GTCTC | 5 cut(s) | 854, 1003, 1076, 1103, 1289 |
| BcuI | ACTAGT | 3 cut(s) | 175, 656, 752 |
| BfaI | CTAG | 4 cut(s) | 176, 657, 753, 1469 |
| BfmI | CTRYAG | 6 cut(s) | 64, 315, 468, 580, 1125, 1323 |
| BfoI | RGCGCY | 1 cut(s) | 615 |
| BfrI | CTTAAG | 1 cut(s) | 836 |
| BisI | GCNGC | 5 cut(s) | 571, 610, 663, 666, 955 |
| BlsI | GCNGC | 5 cut(s) | 572, 611, 664, 667, 956 |
| BmcAI | AGTACT | 1 cut(s) | 539 |
| Bme1390I | CCNGG | 2 cut(s) | 340, 866 |
| Bme18I | GGWCC | 3 cut(s) | 271, 1339, 1378 |
| BmeT110I | CYCGRG | 1 cut(s) | 805 |
| BmgBI | CACGTC | 1 cut(s) | 1213 |
| BmgT120I | GGNCC | 3 cut(s) | 271, 1339, 1378 |
| BmiI | GGNNCC | 3 cut(s) | 1066, 1341, 1380 |
| BmrFI | CCNGG | 2 cut(s) | 340, 866 |
| BoxI | GACNNNNGTC | 1 cut(s) | 866 |
| BpiI | GAAGAC | 1 cut(s) | 115 |
| Bpu10I | CCTNAGC | 3 cut(s) | 455, 904, 1416 |
| Bpu14I | TTCGAA | 2 cut(s) | 144, 498 |
| BpuEI | CTTGAG | 2 cut(s) | 291, 700 |
| BpuMI | CCSGG | 2 cut(s) | 340, 866 |
| BsaAI | YACGTR | 2 cut(s) | 58, 960 |
| BsaHI | GRCGYC | 2 cut(s) | 1038, 1185 |
| BsaI | GGTCTC | 3 cut(s) | 854, 1076, 1103 |
| BsaJI | CCNNGG | 3 cut(s) | 864, 873, 1170 |
| BsaWI | WCCGGW | 2 cut(s) | 247, 1061 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 165, 247, 561, 1025, 1074 |
| Bse1I | ACTGG | 1 cut(s) | 593 |
| Bse21I | CCTNAGG | 1 cut(s) | 560 |
| Bse3DI | GCAATG | 2 cut(s) | 76, 366 |
| BseAI | TCCGGA | 2 cut(s) | 247, 1061 |
| BseDI | CCNNGG | 3 cut(s) | 864, 873, 1170 |
| BseGI | GGATG | 1 cut(s) | 987 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 165, 247, 561, 1025, 1074 |
| BseMI | GCAATG | 2 cut(s) | 76, 366 |
| BseMII | CTCAG | 4 cut(s) | 469, 551, 918, 1407 |
| BseNI | ACTGG | 1 cut(s) | 593 |
| BseRI | GAGGAG | 2 cut(s) | 281, 823 |
| BseSI | GKGCMC | 1 cut(s) | 293 |
| BseX3I | CGGCCG | 2 cut(s) | 663, 1347 |
| BseXI | GCAGC | 2 cut(s) | 621, 966 |
| BseYI | CCCAGC | 1 cut(s) | 413 |
| Bsh1236I | CGCG | 2 cut(s) | 743, 858 |
| Bsh1285I | CGRYCG | 2 cut(s) | 666, 1350 |
| BshFI | GGCC | 6 cut(s) | 158, 404, 665, 878, 968, 1349 |
| BsiEI | CGRYCG | 2 cut(s) | 666, 1350 |
| BsiHKAI | GWGCWC | 2 cut(s) | 194, 293 |
| BsiHKCI | CYCGRG | 1 cut(s) | 805 |
| BsiSI | CCGG | 6 cut(s) | 248, 340, 866, 1062, 1133, 1376 |
| BslFI | GGGAC | 3 cut(s) | 181, 186, 1172 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 165, 247, 561, 1025, 1074 |
| BsmAI | GTCTC | 5 cut(s) | 854, 1003, 1076, 1103, 1289 |
| BsmFI | GGGAC | 3 cut(s) | 181, 186, 1172 |
| BsmI | GAATGC | 1 cut(s) | 847 |
| BsnI | GGCC | 6 cut(s) | 158, 404, 665, 878, 968, 1349 |
| Bso31I | GGTCTC | 3 cut(s) | 854, 1076, 1103 |
| BsoBI | CYCGRG | 1 cut(s) | 805 |
| Bsp119I | TTCGAA | 2 cut(s) | 144, 498 |
| Bsp1286I | GDGCHC | 3 cut(s) | 194, 293, 1422 |
| Bsp13I | TCCGGA | 2 cut(s) | 247, 1061 |
| Bsp1407I | TGTACA | 1 cut(s) | 1260 |
| Bsp143I | GATC | 2 cut(s) | 1064, 1433 |
| Bsp19I | CCATGG | 1 cut(s) | 873 |
| BspACI | CCGC | 7 cut(s) | 570, 573, 662, 666, 856, 882, 1288 |
| BspANI | GGCC | 6 cut(s) | 158, 404, 665, 878, 968, 1349 |
| BspCNI | CTCAG | 4 cut(s) | 468, 552, 917, 1408 |
| BspEI | TCCGGA | 2 cut(s) | 247, 1061 |
| BspFNI | CGCG | 2 cut(s) | 743, 858 |
| BspLI | GGNNCC | 3 cut(s) | 1066, 1341, 1380 |
| BspPI | GGATC | 3 cut(s) | 1059, 1072, 1441 |
| BspQI | GCTCTTC | 1 cut(s) | 786 |
| BspT104I | TTCGAA | 2 cut(s) | 144, 498 |
| BspTI | CTTAAG | 1 cut(s) | 836 |
| BspTNI | GGTCTC | 3 cut(s) | 854, 1076, 1103 |
| BsrBI | CCGCTC | 1 cut(s) | 668 |
| BsrDI | GCAATG | 2 cut(s) | 76, 366 |
| BsrGI | TGTACA | 1 cut(s) | 1260 |
| BsrI | ACTGG | 1 cut(s) | 593 |
| BssECI | CCNNGG | 3 cut(s) | 864, 873, 1170 |
| BssMI | GATC | 2 cut(s) | 1064, 1433 |
| BssNI | GRCGYC | 2 cut(s) | 1038, 1185 |
| BssSI | CACGAG | 1 cut(s) | 976 |
| BssT1I | CCWWGG | 2 cut(s) | 873, 1170 |
| Bst2BI | CACGAG | 1 cut(s) | 976 |
| Bst4CI | ACNGT | 3 cut(s) | 919, 947, 1129 |
| Bst6I | CTCTTC | 1 cut(s) | 786 |
| BstACI | GRCGYC | 2 cut(s) | 1038, 1185 |
| BstAFI | CTTAAG | 1 cut(s) | 836 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1372 |
| BstAUI | TGTACA | 1 cut(s) | 1260 |
| BstBAI | YACGTR | 2 cut(s) | 58, 960 |
| BstBI | TTCGAA | 2 cut(s) | 144, 498 |
| BstC8I | GCNNGC | 3 cut(s) | 892, 970, 1006 |
| BstDEI | CTNAG | 4 cut(s) | 455, 560, 904, 1416 |
| BstDSI | CCRYGG | 1 cut(s) | 873 |
| BstEII | GGTNACC | 1 cut(s) | 868 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 559 |
| BstF5I | GGATG | 1 cut(s) | 987 |
| BstFNI | CGCG | 2 cut(s) | 743, 858 |
| BstH2I | RGCGCY | 1 cut(s) | 615 |
| BstHHI | GCGC | 3 cut(s) | 614, 701, 745 |
| BstKTI | GATC | 2 cut(s) | 1067, 1436 |
| BstMAI | GTCTC | 5 cut(s) | 854, 1003, 1076, 1103, 1289 |
| BstMBI | GATC | 2 cut(s) | 1064, 1433 |
| BstMCI | CGRYCG | 2 cut(s) | 666, 1350 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 793, 1372 |
| BstNSI | RCATGY | 1 cut(s) | 401 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 866 |
| BstPI | GGTNACC | 1 cut(s) | 868 |
| BstSCI | CCNGG | 2 cut(s) | 338, 864 |
| BstSFI | CTRYAG | 6 cut(s) | 64, 315, 468, 580, 1125, 1323 |
| BstSLI | GKGCMC | 1 cut(s) | 293 |
| BstUI | CGCG | 2 cut(s) | 743, 858 |
| BstV1I | GCAGC | 2 cut(s) | 621, 966 |
| BstV2I | GAAGAC | 1 cut(s) | 115 |
| BstX2I | RGATCY | 1 cut(s) | 1064 |
| BstYI | RGATCY | 1 cut(s) | 1064 |
| BstZI | CGGCCG | 2 cut(s) | 663, 1347 |
| Bsu36I | CCTNAGG | 1 cut(s) | 560 |
| BsuRI | GGCC | 6 cut(s) | 158, 404, 665, 878, 968, 1349 |
| BtgI | CCRYGG | 1 cut(s) | 873 |
| BtgZI | GCGATG | 1 cut(s) | 531 |
| BtrI | CACGTC | 1 cut(s) | 1213 |
| BtsCI | GGATG | 1 cut(s) | 987 |
| BtsI | GCAGTG | 1 cut(s) | 1041 |
| BtsIMutI | CAGTG | 3 cut(s) | 915, 1041, 1134 |
| Cac8I | GCNNGC | 3 cut(s) | 892, 970, 1006 |
| CaiI | CAGNNNCTG | 1 cut(s) | 588 |
| CciNI | GCGGCCGC | 1 cut(s) | 663 |
| CfoI | GCGC | 3 cut(s) | 614, 701, 745 |
| Cfr13I | GGNCC | 3 cut(s) | 271, 1339, 1378 |
| Csp6I | GTAC | 6 cut(s) | 55, 238, 538, 756, 1032, 1261 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 381, 416 |
| CviAII | CATG | 6 cut(s) | 92, 350, 398, 781, 874, 1473 |
| CviQI | GTAC | 6 cut(s) | 55, 238, 538, 756, 1032, 1261 |
| DdeI | CTNAG | 4 cut(s) | 455, 560, 904, 1416 |
| DpnI | GATC | 2 cut(s) | 1066, 1435 |
| DpnII | GATC | 2 cut(s) | 1064, 1433 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1385 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1385 |
| EaeI | YGGCCR | 4 cut(s) | 156, 663, 876, 1347 |
| EagI | CGGCCG | 2 cut(s) | 663, 1347 |
| Eam1104I | CTCTTC | 1 cut(s) | 786 |
| EarI | CTCTTC | 1 cut(s) | 786 |
| EclXI | CGGCCG | 2 cut(s) | 663, 1347 |
| Eco130I | CCWWGG | 2 cut(s) | 873, 1170 |
| Eco24I | GRGCYC | 1 cut(s) | 1422 |
| Eco31I | GGTCTC | 3 cut(s) | 854, 1076, 1103 |
| Eco47I | GGWCC | 3 cut(s) | 271, 1339, 1378 |
| Eco52I | CGGCCG | 2 cut(s) | 663, 1347 |
| Eco57I | CTGAAG | 3 cut(s) | 109, 416, 1032 |
| Eco72I | CACGTG | 1 cut(s) | 960 |
| Eco81I | CCTNAGG | 1 cut(s) | 560 |
| Eco88I | CYCGRG | 1 cut(s) | 805 |
| Eco91I | GGTNACC | 1 cut(s) | 868 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 559 |
| EcoO65I | GGTNACC | 1 cut(s) | 868 |
| EcoRI | GAATTC | 1 cut(s) | 923 |
| EcoT14I | CCWWGG | 2 cut(s) | 873, 1170 |
| EcoT38I | GRGCYC | 1 cut(s) | 1422 |
| ErhI | CCWWGG | 2 cut(s) | 873, 1170 |
| FaeI | CATG | 6 cut(s) | 95, 353, 401, 784, 877, 1476 |
| FaqI | GGGAC | 3 cut(s) | 181, 186, 1172 |
| FatI | CATG | 6 cut(s) | 91, 349, 397, 780, 873, 1472 |
| FblI | GTMKAC | 2 cut(s) | 467, 651 |
| Fnu4HI | GCNGC | 5 cut(s) | 571, 610, 663, 666, 955 |
| FokI | GGATG | 1 cut(s) | 994 |
| FriOI | GRGCYC | 1 cut(s) | 1422 |
| Fsp4HI | GCNGC | 5 cut(s) | 571, 610, 663, 666, 955 |
| FspBI | CTAG | 4 cut(s) | 176, 657, 753, 1469 |
| GlaI | GCGC | 3 cut(s) | 613, 700, 744 |
| GluI | GCNGC | 5 cut(s) | 571, 610, 663, 666, 955 |
| GsaI | CCCAGC | 1 cut(s) | 417 |
| HaeII | RGCGCY | 1 cut(s) | 615 |
| HaeIII | GGCC | 6 cut(s) | 158, 404, 665, 878, 968, 1349 |
| HapII | CCGG | 6 cut(s) | 248, 340, 866, 1062, 1133, 1376 |
| HhaI | GCGC | 3 cut(s) | 614, 701, 745 |
| Hin1I | GRCGYC | 2 cut(s) | 1038, 1185 |
| Hin1II | CATG | 6 cut(s) | 95, 353, 401, 784, 877, 1476 |
| Hin6I | GCGC | 3 cut(s) | 612, 699, 743 |
| HinP1I | GCGC | 3 cut(s) | 612, 699, 743 |
| HincII | GTYRAC | 2 cut(s) | 109, 1390 |
| HindII | GTYRAC | 2 cut(s) | 109, 1390 |
| HinfI | GANTC | 8 cut(s) | 74, 80, 113, 260, 481, 813, 1091, 1156 |
| HpaII | CCGG | 6 cut(s) | 248, 340, 866, 1062, 1133, 1376 |
| HphI | GGTGA | 5 cut(s) | 163, 242, 394, 587, 862 |
| Hpy188I | TCNGA | 8 cut(s) | 33, 79, 85, 265, 738, 1096, 1161, 1206 |
| Hpy188III | TCNNGA | 4 cut(s) | 248, 805, 1062, 1431 |
| Hpy99I | CGWCG | 2 cut(s) | 1187, 1348 |
| HpyAV | CCTTC | 6 cut(s) | 415, 440, 516, 725, 958, 1240 |
| HpyCH4III | ACNGT | 3 cut(s) | 919, 947, 1129 |
| HpyCH4IV | ACGT | 6 cut(s) | 57, 374, 959, 1038, 1185, 1212 |
| HpyCH4V | TGCA | 5 cut(s) | 291, 371, 529, 728, 1450 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 793, 1372 |
| HpyF3I | CTNAG | 4 cut(s) | 455, 560, 904, 1416 |
| HpySE526I | ACGT | 6 cut(s) | 57, 374, 959, 1038, 1185, 1212 |
| Hsp92I | GRCGYC | 2 cut(s) | 1038, 1185 |
| Hsp92II | CATG | 6 cut(s) | 95, 353, 401, 784, 877, 1476 |
| HspAI | GCGC | 3 cut(s) | 612, 699, 743 |
| Kpn2I | TCCGGA | 2 cut(s) | 247, 1061 |
| Kzo9I | GATC | 2 cut(s) | 1064, 1433 |
| LguI | GCTCTTC | 1 cut(s) | 786 |
| LmnI | GCTCC | 1 cut(s) | 197 |
| Lsp1109I | GCAGC | 2 cut(s) | 621, 966 |
| MaeI | CTAG | 4 cut(s) | 176, 657, 753, 1469 |
| MaeII | ACGT | 6 cut(s) | 57, 374, 959, 1038, 1185, 1212 |
| MaeIII | GTNAC | 4 cut(s) | 575, 774, 868, 1039 |
| MalI | GATC | 2 cut(s) | 1066, 1435 |
| MbiI | CCGCTC | 1 cut(s) | 668 |
| MboI | GATC | 2 cut(s) | 1064, 1433 |
| MboII | GAAGA | 2 cut(s) | 115, 803 |
| MflI | RGATCY | 1 cut(s) | 1064 |
| MhlI | GDGCHC | 3 cut(s) | 194, 293, 1422 |
| MlsI | TGGCCA | 2 cut(s) | 158, 878 |
| MluCI | AATT | 6 cut(s) | 222, 692, 923, 1014, 1273, 1282 |
| MluNI | TGGCCA | 2 cut(s) | 158, 878 |
| MlyI | GAGTC | 1 cut(s) | 1165 |
| Mox20I | TGGCCA | 2 cut(s) | 158, 878 |
| MroI | TCCGGA | 2 cut(s) | 247, 1061 |
| MroXI | GAANNNNTTC | 1 cut(s) | 122 |
| MscI | TGGCCA | 2 cut(s) | 158, 878 |
| MseI | TTAA | 1 cut(s) | 837 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1445 |
| Msp20I | TGGCCA | 2 cut(s) | 158, 878 |
| MspCI | CTTAAG | 1 cut(s) | 836 |
| MspI | CCGG | 6 cut(s) | 248, 340, 866, 1062, 1133, 1376 |
| MspR9I | CCNGG | 2 cut(s) | 340, 866 |
| Mva1269I | GAATGC | 1 cut(s) | 847 |
| MvnI | CGCG | 2 cut(s) | 743, 858 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 793, 1372 |
| NciI | CCSGG | 2 cut(s) | 340, 866 |
| NcoI | CCATGG | 1 cut(s) | 873 |
| NdeII | GATC | 2 cut(s) | 1064, 1433 |
| NlaIII | CATG | 6 cut(s) | 95, 353, 401, 784, 877, 1476 |
| NlaIV | GGNNCC | 3 cut(s) | 1066, 1341, 1380 |
| NmuCI | GTSAC | 4 cut(s) | 575, 774, 868, 1039 |
| NotI | GCGGCCGC | 1 cut(s) | 663 |
| NspI | RCATGY | 1 cut(s) | 401 |
| NspV | TTCGAA | 2 cut(s) | 144, 498 |
| OliI | CACNNNNGTG | 1 cut(s) | 1445 |
| PaeR7I | CTCGAG | 1 cut(s) | 805 |
| PciSI | GCTCTTC | 1 cut(s) | 786 |
| PctI | GAATGC | 1 cut(s) | 847 |
| PdmI | GAANNNNTTC | 1 cut(s) | 122 |
| PfeI | GAWTC | 7 cut(s) | 74, 80, 113, 260, 481, 813, 1091 |
| PkrI | GCNGC | 5 cut(s) | 572, 611, 664, 667, 956 |
| PleI | GAGTC | 1 cut(s) | 1164 |
| PmaCI | CACGTG | 1 cut(s) | 960 |
| PmlI | CACGTG | 1 cut(s) | 960 |
| PpsI | GAGTC | 1 cut(s) | 1164 |
| Ppu21I | YACGTR | 2 cut(s) | 58, 960 |
| PshAI | GACNNNNGTC | 1 cut(s) | 866 |
| PspCI | CACGTG | 1 cut(s) | 960 |
| PspEI | GGTNACC | 1 cut(s) | 868 |
| PspFI | CCCAGC | 1 cut(s) | 413 |
| PspN4I | GGNNCC | 3 cut(s) | 1066, 1341, 1380 |
| PspPI | GGNCC | 3 cut(s) | 271, 1339, 1378 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 539, 571, 1395, 1427 |
| PstNI | CAGNNNCTG | 1 cut(s) | 588 |
| PsuI | RGATCY | 1 cut(s) | 1064 |
| RsaI | GTAC | 6 cut(s) | 56, 239, 539, 757, 1033, 1262 |
| RsaNI | GTAC | 6 cut(s) | 55, 238, 538, 756, 1032, 1261 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1445 |
| SapI | GCTCTTC | 1 cut(s) | 786 |
| SaqAI | TTAA | 1 cut(s) | 837 |
| SatI | GCNGC | 5 cut(s) | 571, 610, 663, 666, 955 |
| Sau3AI | GATC | 2 cut(s) | 1064, 1433 |
| Sau96I | GGNCC | 3 cut(s) | 271, 1339, 1378 |
| ScaI | AGTACT | 1 cut(s) | 539 |
| SchI | GAGTC | 1 cut(s) | 1165 |
| ScrFI | CCNGG | 2 cut(s) | 340, 866 |
| SduI | GDGCHC | 3 cut(s) | 194, 293, 1422 |
| SfcI | CTRYAG | 6 cut(s) | 64, 315, 468, 580, 1125, 1323 |
| Sfr274I | CTCGAG | 1 cut(s) | 805 |
| SfuI | TTCGAA | 2 cut(s) | 144, 498 |
| SinI | GGWCC | 3 cut(s) | 271, 1339, 1378 |
| SlaI | CTCGAG | 1 cut(s) | 805 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1445 |
| SmlI | CTYRAG | 4 cut(s) | 306, 679, 805, 836 |
| SmoI | CTYRAG | 4 cut(s) | 306, 679, 805, 836 |
| SpeI | ACTAGT | 3 cut(s) | 175, 656, 752 |
| Sse9I | AATT | 6 cut(s) | 222, 692, 923, 1014, 1273, 1282 |
| SsiI | CCGC | 7 cut(s) | 570, 573, 662, 666, 856, 882, 1288 |
| SspI | AATATT | 1 cut(s) | 163 |
| SspMI | CTAG | 4 cut(s) | 176, 657, 753, 1469 |
| StyD4I | CCNGG | 2 cut(s) | 338, 864 |
| StyI | CCWWGG | 2 cut(s) | 873, 1170 |
| TaaI | ACNGT | 3 cut(s) | 919, 947, 1129 |
| TaiI | ACGT | 6 cut(s) | 60, 377, 962, 1041, 1188, 1215 |
| TaqI | TCGA | 6 cut(s) | 116, 144, 330, 498, 617, 806 |
| TaqII | GACCGA | 1 cut(s) | 1375 |
| TasI | AATT | 6 cut(s) | 222, 692, 923, 1014, 1273, 1282 |
| TatI | WGTACW | 2 cut(s) | 537, 1260 |
| TauI | GCSGC | 3 cut(s) | 573, 665, 668 |
| TfiI | GAWTC | 7 cut(s) | 74, 80, 113, 260, 481, 813, 1091 |
| Tru1I | TTAA | 1 cut(s) | 837 |
| Tru9I | TTAA | 1 cut(s) | 837 |
| TscAI | CASTG | 3 cut(s) | 922, 1048, 1134 |
| TseFI | GTSAC | 4 cut(s) | 575, 774, 868, 1039 |
| TseI | GCWGC | 2 cut(s) | 609, 954 |
| Tsp45I | GTSAC | 4 cut(s) | 575, 774, 868, 1039 |
| TspDTI | ATGAA | 3 cut(s) | 273, 1012, 1266 |
| TspGWI | ACGGA | 2 cut(s) | 618, 1321 |
| TspRI | CASTG | 3 cut(s) | 922, 1048, 1134 |
| Vha464I | CTTAAG | 1 cut(s) | 836 |
| VneI | GTGCAC | 1 cut(s) | 289 |
| VpaK11BI | GGWCC | 3 cut(s) | 271, 1339, 1378 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 559 |
| XapI | RAATTY | 3 cut(s) | 692, 923, 1014 |
| XceI | RCATGY | 1 cut(s) | 401 |
| XhoI | CTCGAG | 1 cut(s) | 805 |
| XmiI | GTMKAC | 2 cut(s) | 467, 651 |
| XmnI | GAANNNNTTC | 1 cut(s) | 122 |
| XspI | CTAG | 4 cut(s) | 176, 657, 753, 1469 |
| ZraI | GACGTC | 2 cut(s) | 1039, 1186 |
| ZrmI | AGTACT | 1 cut(s) | 539 |