DNA recombination
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1319 |
| AccBSI | CCGCTC | 1 cut(s) | 294 |
| AccII | CGCG | 1 cut(s) | 575 |
| AclWI | GGATC | 4 cut(s) | 47, 60, 327, 528 |
| AcsI | RAATTY | 3 cut(s) | 803, 1357, 1567 |
| AcuI | CTGAAG | 2 cut(s) | 526, 938 |
| AcyI | GRCGYC | 1 cut(s) | 1320 |
| AfaI | GTAC | 3 cut(s) | 426, 1038, 1416 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 225, 650, 1151, 1408 |
| AgsI | TTSAA | 9 cut(s) | 482, 650, 879, 1048, 1205, 1565, 1579, 1590, 1609 |
| AhlI | ACTAGT | 1 cut(s) | 1157 |
| AjnI | CCWGG | 2 cut(s) | 663, 1315 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 339, 371 |
| AluBI | AGCT | 4 cut(s) | 326, 478, 1582, 1605 |
| AluI | AGCT | 4 cut(s) | 326, 478, 1582, 1605 |
| Alw21I | GWGCWC | 1 cut(s) | 1291 |
| Alw26I | GTCTC | 6 cut(s) | 157, 462, 495, 506, 525, 1198 |
| AlwI | GGATC | 4 cut(s) | 47, 60, 327, 528 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 407 |
| Ama87I | CYCGRG | 2 cut(s) | 254, 503 |
| AoxI | GGCC | 2 cut(s) | 560, 1235 |
| ApoI | RAATTY | 3 cut(s) | 803, 1357, 1567 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 367, 399 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 874 |
| AspLEI | GCGC | 2 cut(s) | 313, 1322 |
| AspS9I | GGNCC | 3 cut(s) | 1018, 1235, 1528 |
| AsuHPI | GGTGA | 1 cut(s) | 793 |
| AvaI | CYCGRG | 2 cut(s) | 254, 503 |
| AvaII | GGWCC | 2 cut(s) | 1018, 1528 |
| BaeGI | GKGCMC | 1 cut(s) | 396 |
| BamHI | GGATCC | 1 cut(s) | 52 |
| BanI | GGYRCC | 1 cut(s) | 1319 |
| BanII | GRGCYC | 1 cut(s) | 267 |
| BbsI | GAAGAC | 1 cut(s) | 666 |
| Bbv12I | GWGCWC | 1 cut(s) | 1291 |
| BbvCI | CCTCAGC | 1 cut(s) | 238 |
| BbvI | GCAGC | 8 cut(s) | 12, 260, 263, 394, 565, 742, 761, 1569 |
| BccI | CCATC | 5 cut(s) | 687, 734, 962, 1246, 1260 |
| BceAI | ACGGC | 2 cut(s) | 13, 96 |
| BciT130I | CCWGG | 2 cut(s) | 665, 1317 |
| BcoDI | GTCTC | 6 cut(s) | 157, 462, 495, 506, 525, 1198 |
| BcuI | ACTAGT | 1 cut(s) | 1157 |
| BfaI | CTAG | 4 cut(s) | 894, 1158, 1284, 1533 |
| BfmI | CTRYAG | 1 cut(s) | 314 |
| BfoI | RGCGCY | 2 cut(s) | 314, 1323 |
| Bme1390I | CCNGG | 2 cut(s) | 665, 1317 |
| Bme18I | GGWCC | 2 cut(s) | 1018, 1528 |
| BmeT110I | CYCGRG | 2 cut(s) | 254, 503 |
| BmgT120I | GGNCC | 3 cut(s) | 1018, 1235, 1528 |
| BmiI | GGNNCC | 7 cut(s) | 54, 183, 825, 931, 1321, 1455, 1530 |
| BmrFI | CCNGG | 2 cut(s) | 665, 1317 |
| BmrI | ACTGGG | 1 cut(s) | 847 |
| BmsI | GCATC | 4 cut(s) | 45, 260, 970, 1430 |
| BmuI | ACTGGG | 1 cut(s) | 847 |
| BpiI | GAAGAC | 1 cut(s) | 666 |
| Bpu10I | CCTNAGC | 2 cut(s) | 238, 1374 |
| BpuEI | CTTGAG | 3 cut(s) | 444, 470, 511 |
| BsaAI | YACGTR | 1 cut(s) | 416 |
| BsaHI | GRCGYC | 1 cut(s) | 1320 |
| BsaJI | CCNNGG | 2 cut(s) | 1315, 1347 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 157, 187 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 225, 650, 1151, 1408 |
| Bse118I | RCCGGY | 1 cut(s) | 307 |
| Bse1I | ACTGG | 2 cut(s) | 842, 1395 |
| Bse3DI | GCAATG | 2 cut(s) | 20, 1404 |
| BseBI | CCWGG | 2 cut(s) | 665, 1317 |
| BseDI | CCNNGG | 2 cut(s) | 1315, 1347 |
| BseGI | GGATG | 3 cut(s) | 275, 337, 1248 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 225, 650, 1151, 1408 |
| BseMI | GCAATG | 2 cut(s) | 20, 1404 |
| BseMII | CTCAG | 4 cut(s) | 252, 625, 711, 1416 |
| BseNI | ACTGG | 2 cut(s) | 842, 1395 |
| BseRI | GAGGAG | 1 cut(s) | 291 |
| BseSI | GKGCMC | 1 cut(s) | 396 |
| BseXI | GCAGC | 8 cut(s) | 12, 260, 263, 394, 565, 742, 761, 1569 |
| BsgI | GTGCAG | 2 cut(s) | 930, 1317 |
| Bsh1236I | CGCG | 1 cut(s) | 575 |
| BshFI | GGCC | 2 cut(s) | 562, 1237 |
| BshNI | GGYRCC | 1 cut(s) | 1319 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1291 |
| BsiHKCI | CYCGRG | 2 cut(s) | 254, 503 |
| BsiSI | CCGG | 2 cut(s) | 179, 308 |
| BslFI | GGGAC | 3 cut(s) | 194, 614, 1514 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 225, 650, 1151, 1408 |
| BsmAI | GTCTC | 6 cut(s) | 157, 462, 495, 506, 525, 1198 |
| BsmBI | CGTCTC | 1 cut(s) | 157 |
| BsmFI | GGGAC | 3 cut(s) | 194, 614, 1514 |
| BsmI | GAATGC | 1 cut(s) | 1585 |
| BsnI | GGCC | 2 cut(s) | 562, 1237 |
| BsoBI | CYCGRG | 2 cut(s) | 254, 503 |
| Bsp1286I | GDGCHC | 3 cut(s) | 267, 396, 1291 |
| Bsp1407I | TGTACA | 1 cut(s) | 1036 |
| Bsp143I | GATC | 4 cut(s) | 52, 319, 470, 520 |
| BspANI | GGCC | 2 cut(s) | 562, 1237 |
| BspCNI | CTCAG | 4 cut(s) | 251, 624, 712, 1417 |
| BspFNI | CGCG | 1 cut(s) | 575 |
| BspLI | GGNNCC | 7 cut(s) | 54, 183, 825, 931, 1321, 1455, 1530 |
| BspPI | GGATC | 4 cut(s) | 47, 60, 327, 528 |
| BspQI | GCTCTTC | 1 cut(s) | 301 |
| BspT107I | GGYRCC | 1 cut(s) | 1319 |
| BsrBI | CCGCTC | 1 cut(s) | 294 |
| BsrDI | GCAATG | 2 cut(s) | 20, 1404 |
| BsrFI | RCCGGY | 1 cut(s) | 307 |
| BsrGI | TGTACA | 1 cut(s) | 1036 |
| BsrI | ACTGG | 2 cut(s) | 842, 1395 |
| BssAI | RCCGGY | 1 cut(s) | 307 |
| BssECI | CCNNGG | 2 cut(s) | 1315, 1347 |
| BssMI | GATC | 4 cut(s) | 52, 319, 470, 520 |
| BssNI | GRCGYC | 1 cut(s) | 1320 |
| Bst2UI | CCWGG | 2 cut(s) | 665, 1317 |
| Bst4CI | ACNGT | 4 cut(s) | 208, 391, 403, 1034 |
| Bst6I | CTCTTC | 3 cut(s) | 160, 301, 1359 |
| BstACI | GRCGYC | 1 cut(s) | 1320 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1187 |
| BstAUI | TGTACA | 1 cut(s) | 1036 |
| BstBAI | YACGTR | 1 cut(s) | 416 |
| BstC8I | GCNNGC | 3 cut(s) | 292, 309, 1274 |
| BstDEI | CTNAG | 5 cut(s) | 238, 611, 720, 1374, 1425 |
| BstF5I | GGATG | 3 cut(s) | 275, 337, 1248 |
| BstFNI | CGCG | 1 cut(s) | 575 |
| BstH2I | RGCGCY | 2 cut(s) | 314, 1323 |
| BstHHI | GCGC | 2 cut(s) | 313, 1322 |
| BstKTI | GATC | 4 cut(s) | 55, 322, 473, 523 |
| BstMAI | GTCTC | 6 cut(s) | 157, 462, 495, 506, 525, 1198 |
| BstMBI | GATC | 4 cut(s) | 52, 319, 470, 520 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 14, 184, 190, 239, 242, 1187, 1300 |
| BstNI | CCWGG | 2 cut(s) | 665, 1317 |
| BstNSI | RCATGY | 1 cut(s) | 1123 |
| BstSCI | CCNGG | 2 cut(s) | 663, 1315 |
| BstSFI | CTRYAG | 1 cut(s) | 314 |
| BstSLI | GKGCMC | 1 cut(s) | 396 |
| BstUI | CGCG | 1 cut(s) | 575 |
| BstV1I | GCAGC | 8 cut(s) | 12, 260, 263, 394, 565, 742, 761, 1569 |
| BstV2I | GAAGAC | 1 cut(s) | 666 |
| BstX2I | RGATCY | 2 cut(s) | 52, 520 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 768 |
| BstYI | RGATCY | 2 cut(s) | 52, 520 |
| BsuRI | GGCC | 2 cut(s) | 562, 1237 |
| BtgZI | GCGATG | 1 cut(s) | 567 |
| BtsCI | GGATG | 3 cut(s) | 275, 337, 1248 |
| BtsI | GCAGTG | 2 cut(s) | 1099, 1289 |
| BtsIMutI | CAGTG | 4 cut(s) | 1039, 1099, 1262, 1289 |
| Cac8I | GCNNGC | 3 cut(s) | 292, 309, 1274 |
| CaiI | CAGNNNCTG | 1 cut(s) | 407 |
| CfoI | GCGC | 2 cut(s) | 313, 1322 |
| Cfr10I | RCCGGY | 1 cut(s) | 307 |
| Cfr13I | GGNCC | 3 cut(s) | 1018, 1235, 1528 |
| CseI | GACGC | 1 cut(s) | 268 |
| Csp6I | GTAC | 3 cut(s) | 425, 1037, 1415 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1553, 1588 |
| CviAII | CATG | 7 cut(s) | 60, 435, 494, 617, 778, 1120, 1520 |
| CviQI | GTAC | 3 cut(s) | 425, 1037, 1415 |
| DdeI | CTNAG | 5 cut(s) | 238, 611, 720, 1374, 1425 |
| DinI | GGCGCC | 1 cut(s) | 1321 |
| DpnI | GATC | 4 cut(s) | 54, 321, 472, 522 |
| DpnII | GATC | 4 cut(s) | 52, 319, 470, 520 |
| Eam1104I | CTCTTC | 3 cut(s) | 160, 301, 1359 |
| EarI | CTCTTC | 3 cut(s) | 160, 301, 1359 |
| EciI | GGCGGA | 3 cut(s) | 98, 165, 277 |
| Eco24I | GRGCYC | 1 cut(s) | 267 |
| Eco32I | GATATC | 1 cut(s) | 952 |
| Eco47I | GGWCC | 2 cut(s) | 1018, 1528 |
| Eco57I | CTGAAG | 2 cut(s) | 526, 938 |
| Eco88I | CYCGRG | 2 cut(s) | 254, 503 |
| EcoRII | CCWGG | 2 cut(s) | 663, 1315 |
| EcoRV | GATATC | 1 cut(s) | 952 |
| EcoT38I | GRGCYC | 1 cut(s) | 267 |
| EgeI | GGCGCC | 1 cut(s) | 1321 |
| EheI | GGCGCC | 1 cut(s) | 1321 |
| Esp3I | CGTCTC | 1 cut(s) | 157 |
| FaeI | CATG | 7 cut(s) | 63, 438, 497, 620, 781, 1123, 1523 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 803, 835 |
| FaqI | GGGAC | 3 cut(s) | 194, 614, 1514 |
| FatI | CATG | 7 cut(s) | 59, 434, 493, 616, 777, 1119, 1519 |
| FauI | CCCGC | 2 cut(s) | 299, 1402 |
| FauNDI | CATATG | 1 cut(s) | 924 |
| FokI | GGATG | 3 cut(s) | 282, 344, 1235 |
| FriOI | GRGCYC | 1 cut(s) | 267 |
| FspBI | CTAG | 4 cut(s) | 894, 1158, 1284, 1533 |
| GlaI | GCGC | 2 cut(s) | 312, 1321 |
| HaeII | RGCGCY | 2 cut(s) | 314, 1323 |
| HaeIII | GGCC | 2 cut(s) | 562, 1237 |
| HapII | CCGG | 2 cut(s) | 179, 308 |
| HgaI | GACGC | 1 cut(s) | 268 |
| HhaI | GCGC | 2 cut(s) | 313, 1322 |
| Hin1I | GRCGYC | 1 cut(s) | 1320 |
| Hin1II | CATG | 7 cut(s) | 63, 438, 497, 620, 781, 1123, 1523 |
| Hin6I | GCGC | 2 cut(s) | 311, 1320 |
| HinP1I | GCGC | 2 cut(s) | 311, 1320 |
| HindIII | AAGCTT | 1 cut(s) | 476 |
| HinfI | GANTC | 4 cut(s) | 602, 887, 1466, 1561 |
| HpaII | CCGG | 2 cut(s) | 179, 308 |
| HphI | GGTGA | 1 cut(s) | 793 |
| Hpy166II | GTNNAC | 3 cut(s) | 1037, 1039, 1382 |
| Hpy188III | TCNNGA | 7 cut(s) | 256, 503, 505, 528, 894, 1152, 1197 |
| Hpy8I | GTNNAC | 3 cut(s) | 1037, 1039, 1382 |
| Hpy99I | CGWCG | 2 cut(s) | 117, 284 |
| HpyAV | CCTTC | 5 cut(s) | 109, 260, 441, 573, 864 |
| HpyCH4III | ACNGT | 4 cut(s) | 208, 391, 403, 1034 |
| HpyCH4IV | ACGT | 1 cut(s) | 415 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 14, 184, 190, 239, 242, 1187, 1300 |
| HpyF3I | CTNAG | 5 cut(s) | 238, 611, 720, 1374, 1425 |
| HpySE526I | ACGT | 1 cut(s) | 415 |
| Hsp92I | GRCGYC | 1 cut(s) | 1320 |
| Hsp92II | CATG | 7 cut(s) | 63, 438, 497, 620, 781, 1123, 1523 |
| HspAI | GCGC | 2 cut(s) | 311, 1320 |
| KasI | GGCGCC | 1 cut(s) | 1319 |
| KroI | GCCGGC | 1 cut(s) | 307 |
| KroNI | GCCGGC | 1 cut(s) | 309 |
| Kzo9I | GATC | 4 cut(s) | 52, 319, 470, 520 |
| LguI | GCTCTTC | 1 cut(s) | 301 |
| LmnI | GCTCC | 6 cut(s) | 22, 181, 763, 829, 1089, 1453 |
| Lsp1109I | GCAGC | 8 cut(s) | 12, 260, 263, 394, 565, 742, 761, 1569 |
| LweI | GCATC | 4 cut(s) | 45, 260, 970, 1430 |
| MaeI | CTAG | 4 cut(s) | 894, 1158, 1284, 1533 |
| MaeII | ACGT | 1 cut(s) | 415 |
| MaeIII | GTNAC | 2 cut(s) | 30, 686 |
| MalI | GATC | 4 cut(s) | 54, 321, 472, 522 |
| MbiI | CCGCTC | 1 cut(s) | 294 |
| MboI | GATC | 4 cut(s) | 52, 319, 470, 520 |
| MboII | GAAGA | 7 cut(s) | 147, 288, 558, 671, 882, 1094, 1376 |
| MflI | RGATCY | 2 cut(s) | 52, 520 |
| MhlI | GDGCHC | 3 cut(s) | 267, 396, 1291 |
| Mly113I | GGCGCC | 1 cut(s) | 1320 |
| MlyI | GAGTC | 2 cut(s) | 596, 1460 |
| MmeI | TCCRAC | 2 cut(s) | 1042, 1045 |
| MroNI | GCCGGC | 1 cut(s) | 307 |
| MroXI | GAANNNNTTC | 1 cut(s) | 874 |
| MseI | TTAA | 4 cut(s) | 90, 441, 624, 1299 |
| MslI | CAYNNNNRTG | 1 cut(s) | 766 |
| MspI | CCGG | 2 cut(s) | 179, 308 |
| MspR9I | CCNGG | 2 cut(s) | 665, 1317 |
| Mva1269I | GAATGC | 1 cut(s) | 1585 |
| MvaI | CCWGG | 2 cut(s) | 665, 1317 |
| MvnI | CGCG | 1 cut(s) | 575 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 14, 184, 190, 239, 242, 1187, 1300 |
| NaeI | GCCGGC | 1 cut(s) | 309 |
| NarI | GGCGCC | 1 cut(s) | 1320 |
| NdeI | CATATG | 1 cut(s) | 924 |
| NdeII | GATC | 4 cut(s) | 52, 319, 470, 520 |
| NgoMIV | GCCGGC | 1 cut(s) | 307 |
| NlaIII | CATG | 7 cut(s) | 63, 438, 497, 620, 781, 1123, 1523 |
| NlaIV | GGNNCC | 7 cut(s) | 54, 183, 825, 931, 1321, 1455, 1530 |
| NmeAIII | GCCGAG | 1 cut(s) | 1328 |
| NmuCI | GTSAC | 2 cut(s) | 30, 686 |
| NspI | RCATGY | 1 cut(s) | 1123 |
| PaeR7I | CTCGAG | 1 cut(s) | 503 |
| PciSI | GCTCTTC | 1 cut(s) | 301 |
| PctI | GAATGC | 1 cut(s) | 1585 |
| PdiI | GCCGGC | 1 cut(s) | 309 |
| PdmI | GAANNNNTTC | 1 cut(s) | 874 |
| PfeI | GAWTC | 2 cut(s) | 887, 1561 |
| PleI | GAGTC | 2 cut(s) | 596, 1460 |
| PluTI | GGCGCC | 1 cut(s) | 1323 |
| PpsI | GAGTC | 2 cut(s) | 596, 1460 |
| Ppu21I | YACGTR | 1 cut(s) | 416 |
| Psp6I | CCWGG | 2 cut(s) | 663, 1315 |
| PspGI | CCWGG | 2 cut(s) | 663, 1315 |
| PspN4I | GGNNCC | 7 cut(s) | 54, 183, 825, 931, 1321, 1455, 1530 |
| PspPI | GGNCC | 3 cut(s) | 1018, 1235, 1528 |
| PstNI | CAGNNNCTG | 1 cut(s) | 407 |
| PsuI | RGATCY | 2 cut(s) | 52, 520 |
| RsaI | GTAC | 3 cut(s) | 426, 1038, 1416 |
| RsaNI | GTAC | 3 cut(s) | 425, 1037, 1415 |
| RseI | CAYNNNNRTG | 1 cut(s) | 766 |
| SapI | GCTCTTC | 1 cut(s) | 301 |
| SaqAI | TTAA | 4 cut(s) | 90, 441, 624, 1299 |
| Sau3AI | GATC | 4 cut(s) | 52, 319, 470, 520 |
| Sau96I | GGNCC | 3 cut(s) | 1018, 1235, 1528 |
| SchI | GAGTC | 2 cut(s) | 596, 1460 |
| ScrFI | CCNGG | 2 cut(s) | 665, 1317 |
| SduI | GDGCHC | 3 cut(s) | 267, 396, 1291 |
| SfaNI | GCATC | 4 cut(s) | 45, 260, 970, 1430 |
| SfcI | CTRYAG | 1 cut(s) | 314 |
| SfoI | GGCGCC | 1 cut(s) | 1321 |
| Sfr274I | CTCGAG | 1 cut(s) | 503 |
| SinI | GGWCC | 2 cut(s) | 1018, 1528 |
| SlaI | CTCGAG | 1 cut(s) | 503 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 766 |
| SmlI | CTYRAG | 4 cut(s) | 459, 485, 503, 526 |
| SmoI | CTYRAG | 4 cut(s) | 459, 485, 503, 526 |
| SpeI | ACTAGT | 1 cut(s) | 1157 |
| SspDI | GGCGCC | 1 cut(s) | 1319 |
| SspMI | CTAG | 4 cut(s) | 894, 1158, 1284, 1533 |
| StyD4I | CCNGG | 2 cut(s) | 663, 1315 |
| TaaI | ACNGT | 4 cut(s) | 208, 391, 403, 1034 |
| TaiI | ACGT | 1 cut(s) | 418 |
| TaqI | TCGA | 4 cut(s) | 504, 537, 1198, 1213 |
| TatI | WGTACW | 1 cut(s) | 1036 |
| TauI | GCSGC | 7 cut(s) | 187, 190, 193, 236, 245, 353, 578 |
| TfiI | GAWTC | 2 cut(s) | 887, 1561 |
| Tru1I | TTAA | 4 cut(s) | 90, 441, 624, 1299 |
| Tru9I | TTAA | 4 cut(s) | 90, 441, 624, 1299 |
| TscAI | CASTG | 4 cut(s) | 1039, 1099, 1262, 1296 |
| TseFI | GTSAC | 2 cut(s) | 30, 686 |
| Tsp45I | GTSAC | 2 cut(s) | 30, 686 |
| TspDTI | ATGAA | 1 cut(s) | 423 |
| TspGWI | ACGGA | 1 cut(s) | 344 |
| TspRI | CASTG | 4 cut(s) | 1039, 1099, 1262, 1296 |
| VpaK11BI | GGWCC | 2 cut(s) | 1018, 1528 |
| XapI | RAATTY | 3 cut(s) | 803, 1357, 1567 |
| XbaI | TCTAGA | 1 cut(s) | 893 |
| XceI | RCATGY | 1 cut(s) | 1123 |
| XhoI | CTCGAG | 1 cut(s) | 503 |
| XmnI | GAANNNNTTC | 1 cut(s) | 874 |
| XspI | CTAG | 4 cut(s) | 894, 1158, 1284, 1533 |