FvH4_5g21501

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000050
Physical Location & Seq
Reverse (-)
12970904 .. 12977875
6972 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g21501.t1

Sequence Viewer

Length: 2178 bp
ATGTCGCCCTACTTTGGCGATCTGATTCGATCTGCTCTTTTGTTGGTTTCCGGCAGGATAAAAATCAAACCGGTTGCAAATTCATCGAAGCAAAGAACACAGAGGCAATACATTGTTCCAGTTTCAGGAAGGAAAGACGATGGACATAACACCGGTGAGAGAAATGGTACCCTTCAGGGAGATTCAGTTCACGCTCTCATCGATGAGGATCAGCACAATGATATAACACAGGCAGTGGAACTAGGACAACTATATGATATATACCGTTTCTCTACGCATCAAGATTTTCTTACATTCAGAGTTCTCCAGAATGAAACGGTAGTTCATTTCAATGGATCAACGGTATTTGTGAAAATAAATGAGGTCTTGCCTCCAGTGCCACAACACGCATTTCGACTTATCGAATTGGAGGACTTGGAAACAGAAAGAGGAAGCAAGGAAATCATGGCAGACGTTTACGGCTGCATTAAAAGCGTCAGAGTACCACATGAACAGCCTCTAAGAAATAAAAATAAAGTGGAGACAAAATGTGAAATCACTGTTCAGAACCTGAGAAAGGAGACCCTTGACATCACTCTTTGGGGAGACATCGCCAGGTCATTTGACATTGGAACTGTGCAAAGATCGTCTCCTCCTGTTCTTACTGTGTTTACCAGCTTGCTTCTAGGAGAATATAATGAATATGTTGTGGCCTCAAACTCAAACCATACATGTGTTTTCTTCAAATCCACCCATTCCCGCCAGAGACGAGTACATGATCGAGTAAGAGACAAGGTGCGCATAATCGGTGCCATCTCCAGTCATCAAACAACTAAAGGTGTTGAACAACCAAACCAAGTGACTGTGTCAGAGTTGAATGCACTCAATCCGATAGATTACATGGAAAAGCCGGTATTCTGCAATGCTTCAATCCGAAGATTCTCACCAAATGGATGGTGGTATAGAAGCTGCTCAACCACCAGCTGTTACAGGATGCTGCACAAAAATGAGGAGAAGGACAATAAGTACTTTTGTCCGCTGCATGGTCTTCAGGACCCGCAACCATCCTATGTGGTCAGGATGAAAATTGAGGATGAAGAGAGCCAGGCCACTTTAACTTTGATGGGGAGACAAGCTCAACAACTTTTCAAAAATAGCTGTCAGGAACTGCTGGAGAAGAGGTTCTACCCAACTGAACAATCATTGCCTGAGGAAATTGTAGACACAGTGGGGCAGGCACACCTATTTCAAATCAAGCTAAAGCCTGATCGCAGTTTCACGGTTAGTGGCATCACTCCAAGCAAAGATGCAGCAACCACAACAGTGGACCTCCCACAAATTGAGAGAAAGAGAACAAATGAGTCAGGCTTGACAACTCTTTCTAAAAAGGAACCGGAAAAGAAGTCCAGGAGAGTACATGTATCAAGGCTGTGGAGGCCTAGGTTCAACAACACTGACACATACGATGGCCTGCACTATGTGTTGGTTGATGAAGATGGCGACTCGATCCACGCTTTCATCAATGAAGACCAGCATGATGATTATATAAAGAAAGTACACCAAGGACAGGTATATGAAATCTGCCTTTTTACCACGCACCTAGCAGATCCCCGTGCGTCCAGAGTTCTTCACAATGACATGGTGGTGCATTTCCATGGACCGACAGAGTTTCTGAAAATAAATGAGGCACCTCTAATCCCACAACAATCATTCCAGCTTATAGAATTCGAGGACCTGGAAATGGAAATAGAAAGAGAGAACAAGGAAGTCTTGACAGGCAAAAGGAAGACCCTCAAGATCACTCTTTGGGGAGACATCGCCAGGTCATTTGACATTGAAACTGTGCAAAGATCACCTTCTCCTGTTCTTGCTGTTTTCACCAGCTTGAATCTAGGAGAATATGATGGACATGTTGTGGCCTCAAACTCAAACCATACATGTGTTTTCTTCAACCCACCCATTCCCGCAACAGACGAGTTCTCCAAAACAGGAGACAACGTGCGCATAATTGGTGCCATCTCCAGCCATCAAACAACTAAAGGTGTTGAACAACCAAACCAAGTGACTGTGTCAGAGTTGAATGCACTCAATCTGATAAATTACATGGAGAAGTCGGTATTCTGCAGTGCTTCGATTCGAAAATTCTCACCAAATGGATGGTGGTATAGGAGCATCATCTTCTTCAAGCATGACGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Helitron_like_N Pkinase Pkinase_Tyr REPA_OB_2 WW

Protein Analysis

726

Amino Acids

82.94

Weight (kDa)

6.54

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_C PF08646 300 - 416 6.9e-10 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000050)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_2g06412 FvH4_2g08146 FvH4_2g10321 FvH4_2g13511 FvH4_2g20780 FvH4_2g20781 FvH4_2g22171 FvH4_2g25221 FvH4_3g03500 FvH4_3g07130 FvH4_3g10140 FvH4_3g10141 FvH4_3g24350 FvH4_4g08242 FvH4_4g13410 FvH4_4g18140 FvH4_4g22470 FvH4_5g19920 FvH4_5g21501 FvH4_5g26612 FvH4_5g29621 FvH4_5g31990 FvH4_6g00280 FvH4_6g00280 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g08820 FvH4_6g29600 FvH4_6g30401 FvH4_6g33630 FvH4_7g08310 FvH4_7g08311 FvH4_7g10871 FvH4_7g18890 FvH4_7g18891
malus_domestica MD01G1202900.v1.1 MD02G1249900.v1.1 MD03G1022200.v1.1 MD03G1164900.v1.1 MD04G1002000.v1.1 MD05G1167800.v1.1 MD05G1167900.v1.1 MD05G1202200.v1.1 MD05G1215800.v1.1 MD05G1328800.v1.1 MD07G1020000.v1.1 MD07G1020100.v1.1 MD07G1084700.v1.1 MD08G1007900.v1.1 MD08G1229500.v1.1 MD09G1016000.v1.1 MD09G1184200.v1.1 MD10G1198800.v1.1 MD10G1198900.v1.1 MD11G1203900.v1.1 MD12G1081100.v1.1 MD12G1101700.v1.1 MD13G1126700.v1.1 MD13G1158300.v1.1 MD13G1161500.v1.1 MD14G1083800.v1.1 MD14G1125700.v1.1 MD14G1159700.v1.1 MD14G1159800.v1.1 MD17G1252600.v1.1 MD17G1261900.v1.1 MD17G1262000.v1.1
pyrus_communis pycom01g12270 pycom02g14690 pycom02g17560 pycom02g26880 pycom02g26890 pycom03g13090 pycom03g19990 pycom03g20000 pycom04g00130 pycom04g05050 pycom04g10460 pycom05g27190 pycom07g05970 pycom08g02820 pycom08g11570 pycom09g07420 pycom09g07430 pycom09g08580 pycom10g06760 pycom10g11210 pycom10g11220 pycom11g14380 pycom11g21990 pycom12419g00230 pycom12494g00020 pycom14g06150 pycom15g09750 pycom15g20130 pycom15g27020 pycom15g27230 pycom16g17550 pycom16g18200 pycom5153684g00260 pycom520g00040
rosa_chinensis RchiOBHm_Chr1g0320331 RchiOBHm_Chr1g0329871 RchiOBHm_Chr1g0339991 RchiOBHm_Chr1g0371171 RchiOBHm_Chr1g0378741 RchiOBHm_Chr1g0378751 RchiOBHm_Chr1g0379271 RchiOBHm_Chr2g0122381 RchiOBHm_Chr2g0133251 RchiOBHm_Chr2g0133271 RchiOBHm_Chr2g0133331 RchiOBHm_Chr2g0135141 RchiOBHm_Chr2g0141711 RchiOBHm_Chr2g0145531 RchiOBHm_Chr2g0149141 RchiOBHm_Chr2g0149151 RchiOBHm_Chr2g0150581 RchiOBHm_Chr2g0159361 RchiOBHm_Chr2g0162161 RchiOBHm_Chr2g0174211 RchiOBHm_Chr3g0484061 RchiOBHm_Chr3g0491361 RchiOBHm_Chr4g0413141 RchiOBHm_Chr4g0444601 RchiOBHm_Chr5g0004901 RchiOBHm_Chr5g0004911 RchiOBHm_Chr5g0010461 RchiOBHm_Chr5g0027941 RchiOBHm_Chr5g0028311 RchiOBHm_Chr5g0030141 RchiOBHm_Chr5g0035621 RchiOBHm_Chr5g0050871 RchiOBHm_Chr5g0053321 RchiOBHm_Chr5g0060091 RchiOBHm_Chr5g0063301 RchiOBHm_Chr5g0066571 RchiOBHm_Chr5g0067561 RchiOBHm_Chr6g0262741 RchiOBHm_Chr7g0184001 RchiOBHm_Chr7g0206181 RchiOBHm_Chr7g0212031 RchiOBHm_Chr7g0214521 RchiOBHm_Chr7g0230521 RchiOBHm_Chr7g0230621
rosa_laevigata RLG00000014424 RLG00000022069 RLG00000036206
rosa_multiflora Rmu_sc0000121.1_g000006 Rmu_sc0000271.1_g000001 Rmu_sc0000293.1_g000010 Rmu_sc0000309.1_g000052 Rmu_sc0000556.1_g000045 Rmu_sc0000837.1_g000079 Rmu_sc0001069.1_g000026 Rmu_sc0001198.1_g000001 Rmu_sc0001353.1_g000031 Rmu_sc0001461.1_g000107 Rmu_sc0002011.1_g000038 Rmu_sc0003270.1_g000034 Rmu_sc0003874.1_g000017 Rmu_sc0004729.1_g000006 Rmu_sc0007116.1_g000001 Rmu_sc0009087.1_g000019 Rmu_sc0009877.1_g000004 Rmu_sc0015060.1_g000008 Rmu_sc0016097.1_g000001
rosa_roxburghii Rroxscaffold_1G00024140 Rroxscaffold_1G00027240 Rroxscaffold_1G00030710 Rroxscaffold_1G00035010 Rroxscaffold_1G00050490 Rroxscaffold_1G00069340 Rroxscaffold_1G00072870 Rroxscaffold_2G00078360 Rroxscaffold_2G00098490 Rroxscaffold_2G00106110 Rroxscaffold_2G00109450 Rroxscaffold_2G00125260 Rroxscaffold_2G00154260 Rroxscaffold_3G00221290 Rroxscaffold_3G00221300 Rroxscaffold_3G00232770 Rroxscaffold_3G00249670 Rroxscaffold_3G00255640 Rroxscaffold_3G00255820 Rroxscaffold_3G00255870 Rroxscaffold_3G00258160 Rroxscaffold_3G00258170 Rroxscaffold_3G00265290 Rroxscaffold_4G00320550 Rroxscaffold_4G00322930 Rroxscaffold_4G00331930 Rroxscaffold_4G00332460 Rroxscaffold_5G00337730 Rroxscaffold_5G00364590 Rroxscaffold_5G00365660 Rroxscaffold_5G00373900 Rroxscaffold_6G00395750 Rroxscaffold_6G00397090 Rroxscaffold_6G00407660 Rroxscaffold_6G00421590 Rroxscaffold_7G00156820 Rroxscaffold_7G00174040 Rroxscaffold_7G00175610 Rroxscaffold_7G00182780 Rroxscaffold_7G00210390 Rroxscaffold_7G00210490 Rroxscaffold_7G00214770 Rroxscaffold_7G00215580 Rroxscaffold_7G00215690
rosa_rugosa Rorug01G0099600 Rorug01G0127300 Rorug02G0145300 Rorug02G0145400 Rorug02G0145500.1 Rorug02G0145600 Rorug02G0145700 Rorug02G0145700 Rorug02G0278400 Rorug02G0444700 Rorug02G0542800 Rorug02G0547200 Rorug04G0015900 Rorug04G0082400 Rorug04G0297900 Rorug05G0078200 Rorug05G0207900 Rorug05G0277000 Rorug05G0528600 Rorug05G0528700 Rorug05G0528800 Rorug06G0001000 Rorug06G0246500 Rorug06G0246600 Rorug06G0246700 Rorug07G0189200 Rorug07G0189300 Rorug07G0189400 Rorug07G0189500 Rorug07G0212200 Rorug07G0212200 Rorug07G0221500 Rorug07G0248800
rosa_samantha Rh1AG011600 Rh1AG052100 Rh1AG163900 Rh1BG006600 Rh1BG025100 Rh1BG025200 Rh1BG032500 Rh1BG374400 Rh1BG374500 Rh1CG153000 Rh1DG148800 Rh1DG160400 Rh1DG181900 Rh1DG405100 Rh1DG445600 Rh2AG005600 Rh2AG137100 Rh2AG150500 Rh2AG150600 Rh2AG243200 Rh2AG264300 Rh2AG349900 Rh2AG387700 Rh2AG485100 Rh2CG115600 Rh2CG269600 Rh2CG294000 Rh2CG294100 Rh2CG317700 Rh2CG375000 Rh2CG471200 Rh2CG611400 Rh2DG070000 Rh2DG073900 Rh2DG234600 Rh3AG258200 Rh3CG251500 Rh3CG283900 Rh3CG331200 Rh3CG371500 Rh3DG077300 Rh3DG185900 Rh3DG332700 Rh3DG360400 Rh4AG105100 Rh4BG191100 Rh4DG139800 Rh4DG186000 Rh4DG187900 Rh5AG302000 Rh5AG431600 Rh5CG090000 Rh5CG214700 Rh5CG299100 Rh5CG561200 Rh5DG205600 Rh5DG244500 Rh5DG257300 Rh5DG257400 Rh5DG325900 Rh5DG335500 Rh5DG359300 Rh5DG364600 Rh5DG388600 Rh6AG013900 Rh6BG045400 Rh6CG031300 Rh6CG069100 Rh6CG157500 Rh6DG029800 Rh6DG066100 Rh6DG260800 Rh6DG320100 Rh7AG007600 Rh7BG046100 Rh7BG053900 Rh7BG204900 Rh7BG322600 Rh7BG421200 Rh7DG063200 Rh7DG233200 Rh7DG287000 Rh7DG289600 Rh7DG323500 Rh7DG323800 Rh7DG323900
rosa_wichuraiana Rw0G001160 Rw0G003580 Rw0G003590 Rw0G005910 Rw0G006230 Rw0G010240 Rw0G011660 Rw0G015630 Rw0G021440 Rw0G023690 Rw1G000060 Rw1G006170 Rw1G007940 Rw1G008630 Rw1G009460 Rw1G013800 Rw1G015100 Rw1G015710 Rw1G016040 Rw1G024780 Rw2G004590 Rw2G026770 Rw2G030550 Rw2G036430 Rw3G011440 Rw3G020600 Rw3G020710 Rw3G025390 Rw3G026320 Rw4G003380 Rw4G016140 Rw4G018350 Rw4G027460 Rw5G004690 Rw5G011140 Rw5G011820 Rw5G016560 Rw5G023230 Rw5G027290 Rw5G028520 Rw5G040540 Rw5G046050 Rw6G013650 Rw6G018020 Rw6G019800 Rw6G023450 Rw7G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 779, 1982
Acc65I GGTACC 1 cut(s) 167
AccB1I GGYRCC 4 cut(s) 167, 788, 1666, 1991
AccI GTMKAC 1 cut(s) 1200
AciI CCGC 4 cut(s) 739, 1016, 1037, 1944
AclWI GGATC 4 cut(s) 216, 343, 1480, 1580
AcsI RAATTY 3 cut(s) 79, 1703, 2120
AcuI CTGAAG 2 cut(s) 158, 1013
AcyI GRCGYC 1 cut(s) 2172
AdeI CACNNNGTG 1 cut(s) 1459
AfaI GTAC 6 cut(s) 169, 483, 753, 1007, 1395, 1536
AfiI CCNNNNNNNGG 6 cut(s) 14, 125, 556, 1022, 1546, 1720
AflIII ACRYGT 4 cut(s) 710, 1396, 1888, 1916
AgeI ACCGGT 2 cut(s) 70, 152
AjnI CCWGG 5 cut(s) 593, 1083, 1385, 1713, 1799
AleI CACNNNNGTG 1 cut(s) 1301
AloI GAACNNNNNNTCC 4 cut(s) 171, 203, 1145, 1177
AluBI AGCT 8 cut(s) 657, 948, 963, 1115, 1137, 1237, 1696, 1863
AluI AGCT 8 cut(s) 657, 948, 963, 1115, 1137, 1237, 1696, 1863
Alw26I GTCTC 9 cut(s) 515, 554, 579, 633, 739, 762, 1102, 1785, 1965
AlwI GGATC 4 cut(s) 216, 343, 1480, 1580
AlwNI CAGNNNCTG 2 cut(s) 550, 1147
AoxI GGCC 5 cut(s) 690, 1086, 1415, 1447, 1896
ApeKI GCWGC 5 cut(s) 462, 948, 976, 1018, 1289
ApoI RAATTY 3 cut(s) 79, 1703, 2120
AsiGI ACCGGT 2 cut(s) 70, 152
Asp700I GAANNNNTTC 1 cut(s) 1160
Asp718I GGTACC 1 cut(s) 167
AspA2I CCTAGG 1 cut(s) 1418
AspLEI GCGC 2 cut(s) 780, 1983
AspS9I GGNCC 4 cut(s) 1033, 1306, 1637, 1711
AsuHPI GGTGA 5 cut(s) 167, 915, 1824, 1849, 2118
AsuII TTCGAA 1 cut(s) 2116
AvaII GGWCC 4 cut(s) 1033, 1306, 1637, 1711
AvrII CCTAGG 1 cut(s) 1418
AxyI CCTNAGG 1 cut(s) 1188
BanI GGYRCC 4 cut(s) 167, 788, 1666, 1991
BarI GAAGNNNNNNTAC 2 cut(s) 1148, 1180
BbsI GAAGAC 3 cut(s) 1019, 1512, 1772
BbvI GCAGC 5 cut(s) 449, 935, 963, 1005, 1301
BceAI ACGGC 1 cut(s) 475
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciT130I CCWGG 5 cut(s) 595, 1085, 1387, 1715, 1801
BcoDI GTCTC 9 cut(s) 515, 554, 579, 633, 739, 762, 1102, 1785, 1965
BfaI CTAG 5 cut(s) 242, 665, 1419, 1580, 1871
BfmI CTRYAG 1 cut(s) 2101
BisI GCNGC 5 cut(s) 463, 949, 977, 1019, 1290
BlnI CCTAGG 1 cut(s) 1418
BlsI GCNGC 5 cut(s) 464, 950, 978, 1020, 1291
BmcAI AGTACT 1 cut(s) 1007
Bme1390I CCNGG 5 cut(s) 595, 1085, 1387, 1715, 1801
Bme18I GGWCC 4 cut(s) 1033, 1306, 1637, 1711
BmgT120I GGNCC 4 cut(s) 1033, 1306, 1637, 1711
BmiI GGNNCC 6 cut(s) 169, 790, 1035, 1371, 1668, 1993
BmrFI CCNGG 5 cut(s) 595, 1085, 1387, 1715, 1801
BmsI GCATC 5 cut(s) 286, 963, 1276, 1278, 2160
BpiI GAAGAC 3 cut(s) 1019, 1512, 1772
BplI GAGNNNNNCTC 2 cut(s) 1099, 1131
BpmI CTGGAG 5 cut(s) 290, 357, 781, 1172, 1984
Bpu14I TTCGAA 1 cut(s) 2116
BpuEI CTTGAG 1 cut(s) 1757
Bsa29I ATCGAT 1 cut(s) 201
BsaBI GATNNNNATC 2 cut(s) 62, 207
BsaHI GRCGYC 1 cut(s) 2172
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 3 cut(s) 1418, 1540, 1633
BsaWI WCCGGW 3 cut(s) 70, 152, 1372
BsaXI ACNNNNNCTCC 8 cut(s) 171, 201, 1145, 1175, 1943, 1962, 1973, 1992
Bsc4I CCNNNNNNNGG 6 cut(s) 14, 125, 556, 1022, 1546, 1720
Bse118I RCCGGY 3 cut(s) 70, 152, 889
Bse1I ACTGG 3 cut(s) 119, 374, 798
Bse21I CCTNAGG 1 cut(s) 1188
Bse3DI GCAATG 2 cut(s) 907, 1181
Bse8I GATNNNNATC 2 cut(s) 62, 207
BseBI CCWGG 5 cut(s) 595, 1085, 1387, 1715, 1801
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 3 cut(s) 1418, 1540, 1633
BseGI GGATG 6 cut(s) 938, 978, 1043, 1065, 1078, 2141
BseJI GATNNNNATC 2 cut(s) 62, 207
BseLI CCNNNNNNNGG 6 cut(s) 14, 125, 556, 1022, 1546, 1720
BseMI GCAATG 2 cut(s) 907, 1181
BseMII CTCAG 2 cut(s) 542, 1179
BseNI ACTGG 3 cut(s) 119, 374, 798
BseRI GAGGAG 2 cut(s) 621, 1004
BseXI GCAGC 5 cut(s) 449, 935, 963, 1005, 1301
BsgI GTGCAG 2 cut(s) 962, 1436
BshFI GGCC 5 cut(s) 692, 1088, 1417, 1449, 1898
BshNI GGYRCC 4 cut(s) 167, 788, 1666, 1991
BshTI ACCGGT 2 cut(s) 70, 152
BshVI ATCGAT 1 cut(s) 201
BsiSI CCGG 5 cut(s) 51, 71, 153, 890, 1373
BslI CCNNNNNNNGG 6 cut(s) 14, 125, 556, 1022, 1546, 1720
BsmAI GTCTC 9 cut(s) 515, 554, 579, 633, 739, 762, 1102, 1785, 1965
BsmBI CGTCTC 2 cut(s) 633, 739
BsmI GAATGC 2 cut(s) 862, 2065
BsnI GGCC 5 cut(s) 692, 1088, 1417, 1449, 1898
Bso31I GGTCTC 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 2116
Bsp19I CCATGG 1 cut(s) 1633
BspACI CCGC 4 cut(s) 739, 1016, 1037, 1944
BspANI GGCC 5 cut(s) 692, 1088, 1417, 1449, 1898
BspCNI CTCAG 2 cut(s) 543, 1180
BspDI ATCGAT 1 cut(s) 201
BspLI GGNNCC 6 cut(s) 169, 790, 1035, 1371, 1668, 1993
BspMAI CTGCAG 1 cut(s) 2105
BspPI GGATC 4 cut(s) 216, 343, 1480, 1580
BspT104I TTCGAA 1 cut(s) 2116
BspT107I GGYRCC 4 cut(s) 167, 788, 1666, 1991
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 2 cut(s) 907, 1181
BsrFI RCCGGY 3 cut(s) 70, 152, 889
BsrI ACTGG 3 cut(s) 119, 374, 798
BssAI RCCGGY 3 cut(s) 70, 152, 889
BssECI CCNNGG 3 cut(s) 1418, 1540, 1633
BssNI GRCGYC 1 cut(s) 2172
BssT1I CCWWGG 3 cut(s) 1418, 1540, 1633
Bst2UI CCWGG 5 cut(s) 595, 1085, 1387, 1715, 1801
Bst6I CTCTTC 2 cut(s) 1071, 1151
BstACI GRCGYC 1 cut(s) 2172
BstBI TTCGAA 1 cut(s) 2116
BstC8I GCNNGC 3 cut(s) 659, 1215, 1451
BstDEI CTNAG 3 cut(s) 500, 551, 1188
BstDSI CCRYGG 1 cut(s) 1633
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 6 cut(s) 938, 978, 1043, 1065, 1078, 2141
BstHHI GCGC 2 cut(s) 780, 1983
BstMAI GTCTC 9 cut(s) 515, 554, 579, 633, 739, 762, 1102, 1785, 1965
BstMWI GCNNNNNNNGC 3 cut(s) 376, 471, 1414
BstNI CCWGG 5 cut(s) 595, 1085, 1387, 1715, 1801
BstNSI RCATGY 4 cut(s) 714, 1400, 1892, 1920
BstSCI CCNGG 5 cut(s) 593, 1083, 1385, 1713, 1799
BstSFI CTRYAG 1 cut(s) 2101
BstV1I GCAGC 5 cut(s) 449, 935, 963, 1005, 1301
BstV2I GAAGAC 3 cut(s) 1019, 1512, 1772
BstX2I RGATCY 1 cut(s) 1585
BstXI CCANNNNNNTGG 3 cut(s) 933, 1303, 2136
BstYI RGATCY 1 cut(s) 1585
Bsu15I ATCGAT 1 cut(s) 201
Bsu36I CCTNAGG 1 cut(s) 1188
BsuRI GGCC 5 cut(s) 692, 1088, 1417, 1449, 1898
BsuTUI ATCGAT 1 cut(s) 201
BtgI CCRYGG 1 cut(s) 1633
BtgZI GCGATG 2 cut(s) 574, 1780
BtsCI GGATG 6 cut(s) 938, 978, 1043, 1065, 1078, 2141
BtsI GCAGTG 2 cut(s) 240, 2110
BtsIMutI CAGTG 7 cut(s) 240, 381, 537, 1212, 1308, 1431, 2110
Cac8I GCNNGC 3 cut(s) 659, 1215, 1451
CaiI CAGNNNCTG 2 cut(s) 550, 1147
CfoI GCGC 2 cut(s) 780, 1983
Cfr10I RCCGGY 3 cut(s) 70, 152, 889
Cfr13I GGNCC 4 cut(s) 1033, 1306, 1637, 1711
ClaI ATCGAT 1 cut(s) 201
CseI GACGC 2 cut(s) 463, 1584
Csp6I GTAC 6 cut(s) 168, 482, 752, 1006, 1394, 1535
CspAI ACCGGT 2 cut(s) 70, 152
CviQI GTAC 6 cut(s) 168, 482, 752, 1006, 1394, 1535
DdeI CTNAG 3 cut(s) 500, 551, 1188
DraIII CACNNNGTG 1 cut(s) 1459
Eam1104I CTCTTC 2 cut(s) 1071, 1151
EarI CTCTTC 2 cut(s) 1071, 1151
Eco130I CCWWGG 3 cut(s) 1418, 1540, 1633
Eco147I AGGCCT 1 cut(s) 1417
Eco31I GGTCTC 1 cut(s) 554
Eco47I GGWCC 4 cut(s) 1033, 1306, 1637, 1711
Eco57I CTGAAG 2 cut(s) 158, 1013
Eco81I CCTNAGG 1 cut(s) 1188
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 2 cut(s) 1033, 1711
EcoRI GAATTC 1 cut(s) 1703
EcoRII CCWGG 5 cut(s) 593, 1083, 1385, 1713, 1799
EcoT14I CCWWGG 3 cut(s) 1418, 1540, 1633
ErhI CCWWGG 3 cut(s) 1418, 1540, 1633
Esp3I CGTCTC 2 cut(s) 633, 739
FalI AAGNNNNNCTT 6 cut(s) 273, 305, 1733, 1765, 1819, 1851
FauI CCCGC 3 cut(s) 746, 1044, 1951
FblI GTMKAC 1 cut(s) 1200
Fnu4HI GCNGC 5 cut(s) 463, 949, 977, 1019, 1290
FokI GGATG 6 cut(s) 945, 985, 1030, 1072, 1085, 2148
Fsp4HI GCNGC 5 cut(s) 463, 949, 977, 1019, 1290
FspAI RTGCGCAY 2 cut(s) 779, 1982
FspBI CTAG 5 cut(s) 242, 665, 1419, 1580, 1871
FspI TGCGCA 2 cut(s) 779, 1982
GlaI GCGC 2 cut(s) 779, 1982
GluI GCNGC 5 cut(s) 463, 949, 977, 1019, 1290
GsuI CTGGAG 5 cut(s) 290, 357, 781, 1172, 1984
HaeIII GGCC 5 cut(s) 692, 1088, 1417, 1449, 1898
HapII CCGG 5 cut(s) 51, 71, 153, 890, 1373
HgaI GACGC 2 cut(s) 463, 1584
HhaI GCGC 2 cut(s) 780, 1983
Hin1I GRCGYC 1 cut(s) 2172
Hin6I GCGC 2 cut(s) 778, 1981
HinP1I GCGC 2 cut(s) 778, 1981
HinfI GANTC 7 cut(s) 25, 182, 918, 1340, 1481, 1867, 2113
HpaII CCGG 5 cut(s) 51, 71, 153, 890, 1373
HphI GGTGA 5 cut(s) 167, 915, 1824, 1849, 2118
Hpy166II GTNNAC 6 cut(s) 190, 457, 651, 1201, 1306, 1537
Hpy188III TCNNGA 9 cut(s) 126, 281, 307, 1031, 1057, 1142, 1599, 1750, 1774
Hpy8I GTNNAC 6 cut(s) 190, 457, 651, 1201, 1306, 1537
HpyAV CCTTC 4 cut(s) 123, 182, 988, 1845
HpyCH4IV ACGT 2 cut(s) 453, 1977
HpyF10VI GCNNNNNNNGC 3 cut(s) 376, 471, 1414
HpyF3I CTNAG 3 cut(s) 500, 551, 1188
HpySE526I ACGT 2 cut(s) 453, 1977
Hsp92I GRCGYC 1 cut(s) 2172
HspAI GCGC 2 cut(s) 778, 1981
KpnI GGTACC 1 cut(s) 171
LmnI GCTCC 1 cut(s) 2148
Lsp1109I GCAGC 5 cut(s) 449, 935, 963, 1005, 1301
LweI GCATC 5 cut(s) 286, 963, 1276, 1278, 2160
MaeI CTAG 5 cut(s) 242, 665, 1419, 1580, 1871
MaeII ACGT 2 cut(s) 453, 1977
MaeIII GTNAC 3 cut(s) 838, 965, 2041
MflI RGATCY 1 cut(s) 1585
MluCI AATT 9 cut(s) 79, 404, 1065, 1194, 1317, 1703, 1986, 2077, 2120
MlyI GAGTC 2 cut(s) 1349, 1475
MroXI GAANNNNTTC 1 cut(s) 1160
MseI TTAA 2 cut(s) 468, 1094
MslI CAYNNNNRTG 7 cut(s) 330, 711, 984, 1301, 1622, 1632, 1917
MspA1I CMGCKG 2 cut(s) 963, 1018
MspI CCGG 5 cut(s) 51, 71, 153, 890, 1373
MspR9I CCNGG 5 cut(s) 595, 1085, 1387, 1715, 1801
Mva1269I GAATGC 2 cut(s) 862, 2065
MvaI CCWGG 5 cut(s) 595, 1085, 1387, 1715, 1801
MwoI GCNNNNNNNGC 3 cut(s) 376, 471, 1414
NcoI CCATGG 1 cut(s) 1633
NlaIV GGNNCC 6 cut(s) 169, 790, 1035, 1371, 1668, 1993
NmuCI GTSAC 2 cut(s) 838, 2041
NsbI TGCGCA 2 cut(s) 779, 1982
NspI RCATGY 4 cut(s) 714, 1400, 1892, 1920
NspV TTCGAA 1 cut(s) 2116
OliI CACNNNNGTG 1 cut(s) 1301
PceI AGGCCT 1 cut(s) 1417
PciI ACATGT 4 cut(s) 710, 1396, 1888, 1916
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 2 cut(s) 862, 2065
PdmI GAANNNNTTC 1 cut(s) 1160
PfeI GAWTC 5 cut(s) 25, 182, 918, 1867, 2113
PflFI GACNNNGTC 2 cut(s) 844, 2047
PfoI TCCNGGA 1 cut(s) 1385
PinAI ACCGGT 2 cut(s) 70, 152
PkrI GCNGC 5 cut(s) 464, 950, 978, 1020, 1291
PleI GAGTC 2 cut(s) 1348, 1475
PpsI GAGTC 2 cut(s) 1348, 1475
PpuMI RGGWCCY 2 cut(s) 1033, 1711
PscI ACATGT 4 cut(s) 710, 1396, 1888, 1916
Psp5II RGGWCCY 2 cut(s) 1033, 1711
Psp6I CCWGG 5 cut(s) 593, 1083, 1385, 1713, 1799
PspGI CCWGG 5 cut(s) 593, 1083, 1385, 1713, 1799
PspN4I GGNNCC 6 cut(s) 169, 790, 1035, 1371, 1668, 1993
PspPI GGNCC 4 cut(s) 1033, 1306, 1637, 1711
PspPPI RGGWCCY 2 cut(s) 1033, 1711
PstI CTGCAG 1 cut(s) 2105
PstNI CAGNNNCTG 2 cut(s) 550, 1147
PsuI RGATCY 1 cut(s) 1585
PsyI GACNNNGTC 2 cut(s) 844, 2047
PvuII CAGCTG 1 cut(s) 963
RsaI GTAC 6 cut(s) 169, 483, 753, 1007, 1395, 1536
RsaNI GTAC 6 cut(s) 168, 482, 752, 1006, 1394, 1535
RseI CAYNNNNRTG 7 cut(s) 330, 711, 984, 1301, 1622, 1632, 1917
SaqAI TTAA 2 cut(s) 468, 1094
SatI GCNGC 5 cut(s) 463, 949, 977, 1019, 1290
Sau96I GGNCC 4 cut(s) 1033, 1306, 1637, 1711
ScaI AGTACT 1 cut(s) 1007
SchI GAGTC 2 cut(s) 1349, 1475
ScrFI CCNGG 5 cut(s) 595, 1085, 1387, 1715, 1801
SfaNI GCATC 5 cut(s) 286, 963, 1276, 1278, 2160
SfcI CTRYAG 1 cut(s) 2101
SfuI TTCGAA 1 cut(s) 2116
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 4 cut(s) 1033, 1306, 1637, 1711
SmiMI CAYNNNNRTG 7 cut(s) 330, 711, 984, 1301, 1622, 1632, 1917
SmlI CTYRAG 1 cut(s) 1772
SmoI CTYRAG 1 cut(s) 1772
Sse9I AATT 9 cut(s) 79, 404, 1065, 1194, 1317, 1703, 1986, 2077, 2120
SseBI AGGCCT 1 cut(s) 1417
SsiI CCGC 4 cut(s) 739, 1016, 1037, 1944
SspMI CTAG 5 cut(s) 242, 665, 1419, 1580, 1871
StuI AGGCCT 1 cut(s) 1417
StyD4I CCNGG 5 cut(s) 593, 1083, 1385, 1713, 1799
StyI CCWWGG 3 cut(s) 1418, 1540, 1633
TaiI ACGT 2 cut(s) 456, 1980
TaqII GACCGA 1 cut(s) 1654
TasI AATT 9 cut(s) 79, 404, 1065, 1194, 1317, 1703, 1986, 2077, 2120
TatI WGTACW 4 cut(s) 751, 1005, 1393, 1534
TfiI GAWTC 5 cut(s) 25, 182, 918, 1867, 2113
Tru1I TTAA 2 cut(s) 468, 1094
Tru9I TTAA 2 cut(s) 468, 1094
TscAI CASTG 7 cut(s) 240, 381, 544, 1212, 1308, 1438, 2110
TseFI GTSAC 2 cut(s) 838, 2041
TseI GCWGC 5 cut(s) 462, 948, 976, 1018, 1289
Tsp45I GTSAC 2 cut(s) 838, 2041
TspRI CASTG 7 cut(s) 240, 381, 544, 1212, 1308, 1438, 2110
Tth111I GACNNNGTC 2 cut(s) 844, 2047
VpaK11BI GGWCC 4 cut(s) 1033, 1306, 1637, 1711
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 3 cut(s) 79, 1703, 2120
XceI RCATGY 4 cut(s) 714, 1400, 1892, 1920
XcmI CCANNNNNNNNNTGG 2 cut(s) 933, 2136
XmaJI CCTAGG 1 cut(s) 1418
XmiI GTMKAC 1 cut(s) 1200
XmnI GAANNNNTTC 1 cut(s) 1160
XspI CTAG 5 cut(s) 242, 665, 1419, 1580, 1871
ZrmI AGTACT 1 cut(s) 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.