DNA recombination
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 516 |
| Acc36I | ACCTGC | 1 cut(s) | 641 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 302 |
| AccI | GTMKAC | 1 cut(s) | 723 |
| AciI | CCGC | 2 cut(s) | 352, 637 |
| AclWI | GGATC | 1 cut(s) | 287 |
| AcoI | YGGCCR | 1 cut(s) | 304 |
| AcsI | RAATTY | 2 cut(s) | 329, 861 |
| AfaI | GTAC | 6 cut(s) | 54, 207, 462, 542, 617, 696 |
| AfeI | AGCGCT | 1 cut(s) | 561 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 302 |
| AgsI | TTSAA | 3 cut(s) | 245, 505, 837 |
| AjnI | CCWGG | 1 cut(s) | 456 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 777, 809 |
| AluBI | AGCT | 5 cut(s) | 225, 279, 525, 673, 678 |
| AluI | AGCT | 5 cut(s) | 225, 279, 525, 673, 678 |
| Alw21I | GWGCWC | 2 cut(s) | 269, 768 |
| AlwI | GGATC | 1 cut(s) | 287 |
| Aor51HI | AGCGCT | 1 cut(s) | 561 |
| AoxI | GGCC | 1 cut(s) | 304 |
| ApeKI | GCWGC | 4 cut(s) | 36, 279, 522, 798 |
| ApoI | RAATTY | 2 cut(s) | 329, 861 |
| AspLEI | GCGC | 1 cut(s) | 562 |
| AsuHPI | GGTGA | 1 cut(s) | 890 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 607, 640 |
| BalI | TGGCCA | 1 cut(s) | 306 |
| BbsI | GAAGAC | 2 cut(s) | 321, 781 |
| Bbv12I | GWGCWC | 2 cut(s) | 269, 768 |
| BbvI | GCAGC | 4 cut(s) | 23, 266, 534, 810 |
| BccI | CCATC | 2 cut(s) | 268, 379 |
| BceAI | ACGGC | 1 cut(s) | 443 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 161, 195 |
| BciT130I | CCWGG | 1 cut(s) | 458 |
| BciVI | GTATCC | 1 cut(s) | 148 |
| BfaI | CTAG | 2 cut(s) | 209, 222 |
| BfmI | CTRYAG | 1 cut(s) | 679 |
| BfoI | RGCGCY | 1 cut(s) | 563 |
| BfuAI | ACCTGC | 1 cut(s) | 641 |
| BfuI | GTATCC | 1 cut(s) | 148 |
| BisI | GCNGC | 5 cut(s) | 37, 280, 523, 637, 799 |
| BlpI | GCTNAGC | 1 cut(s) | 674 |
| BlsI | GCNGC | 5 cut(s) | 38, 281, 524, 638, 800 |
| BmcAI | AGTACT | 1 cut(s) | 207 |
| Bme1390I | CCNGG | 1 cut(s) | 458 |
| BmrFI | CCNGG | 1 cut(s) | 458 |
| BmsI | GCATC | 2 cut(s) | 48, 169 |
| BpiI | GAAGAC | 2 cut(s) | 321, 781 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 220, 252 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 674 |
| BpuEI | CTTGAG | 2 cut(s) | 247, 632 |
| BsaBI | GATNNNNATC | 1 cut(s) | 666 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 302 |
| Bse1I | ACTGG | 1 cut(s) | 203 |
| Bse3DI | GCAATG | 2 cut(s) | 545, 577 |
| Bse8I | GATNNNNATC | 1 cut(s) | 666 |
| BseBI | CCWGG | 1 cut(s) | 458 |
| BseGI | GGATG | 2 cut(s) | 184, 371 |
| BseJI | GATNNNNATC | 1 cut(s) | 666 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 302 |
| BseMI | GCAATG | 2 cut(s) | 545, 577 |
| BseMII | CTCAG | 1 cut(s) | 688 |
| BseNI | ACTGG | 1 cut(s) | 203 |
| BseXI | GCAGC | 4 cut(s) | 23, 266, 534, 810 |
| BshFI | GGCC | 1 cut(s) | 306 |
| BsiHKAI | GWGCWC | 2 cut(s) | 269, 768 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 302 |
| BsnI | GGCC | 1 cut(s) | 306 |
| Bsp1286I | GDGCHC | 2 cut(s) | 269, 768 |
| Bsp1407I | TGTACA | 1 cut(s) | 540 |
| Bsp143I | GATC | 3 cut(s) | 172, 292, 403 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 674 |
| BspACI | CCGC | 2 cut(s) | 352, 637 |
| BspANI | GGCC | 1 cut(s) | 306 |
| BspCNI | CTCAG | 1 cut(s) | 687 |
| BspHI | TCATGA | 2 cut(s) | 61, 768 |
| BspMI | ACCTGC | 1 cut(s) | 641 |
| BspPI | GGATC | 1 cut(s) | 287 |
| BsrDI | GCAATG | 2 cut(s) | 545, 577 |
| BsrGI | TGTACA | 1 cut(s) | 540 |
| BsrI | ACTGG | 1 cut(s) | 203 |
| BssMI | GATC | 3 cut(s) | 172, 292, 403 |
| BssNAI | GTATAC | 1 cut(s) | 724 |
| Bst1107I | GTATAC | 1 cut(s) | 724 |
| Bst2UI | CCWGG | 1 cut(s) | 458 |
| Bst4CI | ACNGT | 7 cut(s) | 16, 439, 540, 605, 760, 789, 850 |
| BstAUI | TGTACA | 1 cut(s) | 540 |
| BstDEI | CTNAG | 1 cut(s) | 674 |
| BstF5I | GGATG | 2 cut(s) | 184, 371 |
| BstH2I | RGCGCY | 1 cut(s) | 563 |
| BstHHI | GCGC | 1 cut(s) | 562 |
| BstKTI | GATC | 3 cut(s) | 175, 295, 406 |
| BstMBI | GATC | 3 cut(s) | 172, 292, 403 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 358, 633 |
| BstNI | CCWGG | 1 cut(s) | 458 |
| BstNSI | RCATGY | 2 cut(s) | 511, 832 |
| BstSCI | CCNGG | 1 cut(s) | 456 |
| BstSFI | CTRYAG | 1 cut(s) | 679 |
| BstV1I | GCAGC | 4 cut(s) | 23, 266, 534, 810 |
| BstV2I | GAAGAC | 2 cut(s) | 321, 781 |
| BstZ17I | GTATAC | 1 cut(s) | 724 |
| BsuI | GTATCC | 1 cut(s) | 148 |
| BsuRI | GGCC | 1 cut(s) | 306 |
| BtsCI | GGATG | 2 cut(s) | 184, 371 |
| BveI | ACCTGC | 1 cut(s) | 641 |
| CciI | TCATGA | 2 cut(s) | 61, 768 |
| CfoI | GCGC | 1 cut(s) | 562 |
| CseI | GACGC | 1 cut(s) | 411 |
| CsiI | ACCWGGT | 1 cut(s) | 456 |
| Csp6I | GTAC | 6 cut(s) | 53, 206, 461, 541, 616, 695 |
| CviAII | CATG | 9 cut(s) | 62, 407, 449, 508, 576, 711, 727, 769, 829 |
| CviQI | GTAC | 6 cut(s) | 53, 206, 461, 541, 616, 695 |
| DdeI | CTNAG | 1 cut(s) | 674 |
| DpnI | GATC | 3 cut(s) | 174, 294, 405 |
| DpnII | GATC | 3 cut(s) | 172, 292, 403 |
| EaeI | YGGCCR | 1 cut(s) | 304 |
| Eco47III | AGCGCT | 1 cut(s) | 561 |
| EcoRI | GAATTC | 1 cut(s) | 329 |
| EcoRII | CCWGG | 1 cut(s) | 456 |
| EcoT22I | ATGCAT | 1 cut(s) | 577 |
| FaeI | CATG | 9 cut(s) | 65, 410, 452, 511, 579, 714, 730, 772, 832 |
| FatI | CATG | 9 cut(s) | 61, 406, 448, 507, 575, 710, 726, 768, 828 |
| FblI | GTMKAC | 1 cut(s) | 723 |
| Fnu4HI | GCNGC | 5 cut(s) | 37, 280, 523, 637, 799 |
| FokI | GGATG | 2 cut(s) | 191, 358 |
| Fsp4HI | GCNGC | 5 cut(s) | 37, 280, 523, 637, 799 |
| FspBI | CTAG | 2 cut(s) | 209, 222 |
| GlaI | GCGC | 1 cut(s) | 561 |
| GluI | GCNGC | 5 cut(s) | 37, 280, 523, 637, 799 |
| HaeII | RGCGCY | 1 cut(s) | 563 |
| HaeIII | GGCC | 1 cut(s) | 306 |
| HgaI | GACGC | 1 cut(s) | 411 |
| HhaI | GCGC | 1 cut(s) | 562 |
| Hin1II | CATG | 9 cut(s) | 65, 410, 452, 511, 579, 714, 730, 772, 832 |
| Hin6I | GCGC | 1 cut(s) | 560 |
| HinP1I | GCGC | 1 cut(s) | 560 |
| HincII | GTYRAC | 1 cut(s) | 820 |
| HindII | GTYRAC | 1 cut(s) | 820 |
| HphI | GGTGA | 1 cut(s) | 890 |
| Hpy166II | GTNNAC | 3 cut(s) | 543, 724, 820 |
| Hpy188I | TCNGA | 1 cut(s) | 867 |
| Hpy188III | TCNNGA | 5 cut(s) | 62, 176, 296, 769, 809 |
| Hpy8I | GTNNAC | 3 cut(s) | 543, 724, 820 |
| HpyCH4III | ACNGT | 7 cut(s) | 16, 439, 540, 605, 760, 789, 850 |
| HpyCH4IV | ACGT | 1 cut(s) | 693 |
| HpyCH4V | TGCA | 9 cut(s) | 39, 182, 282, 410, 448, 522, 550, 570, 575 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 358, 633 |
| HpyF3I | CTNAG | 1 cut(s) | 674 |
| HpySE526I | ACGT | 1 cut(s) | 693 |
| Hsp92II | CATG | 9 cut(s) | 65, 410, 452, 511, 579, 714, 730, 772, 832 |
| HspAI | GCGC | 1 cut(s) | 560 |
| Kzo9I | GATC | 3 cut(s) | 172, 292, 403 |
| Lsp1109I | GCAGC | 4 cut(s) | 23, 266, 534, 810 |
| LweI | GCATC | 2 cut(s) | 48, 169 |
| MabI | ACCWGGT | 1 cut(s) | 456 |
| MaeI | CTAG | 2 cut(s) | 209, 222 |
| MaeII | ACGT | 1 cut(s) | 693 |
| MaeIII | GTNAC | 2 cut(s) | 423, 713 |
| MalI | GATC | 3 cut(s) | 174, 294, 405 |
| MboI | GATC | 3 cut(s) | 172, 292, 403 |
| MboII | GAAGA | 6 cut(s) | 141, 149, 326, 338, 671, 786 |
| MfeI | CAATTG | 2 cut(s) | 732, 873 |
| MhlI | GDGCHC | 2 cut(s) | 269, 768 |
| MlsI | TGGCCA | 1 cut(s) | 306 |
| MluCI | AATT | 6 cut(s) | 183, 190, 329, 732, 861, 873 |
| MluNI | TGGCCA | 1 cut(s) | 306 |
| MmeI | TCCRAC | 1 cut(s) | 509 |
| MnlI | CCTC | 5 cut(s) | 207, 222, 316, 492, 714 |
| Mox20I | TGGCCA | 1 cut(s) | 306 |
| Mph1103I | ATGCAT | 1 cut(s) | 577 |
| MscI | TGGCCA | 1 cut(s) | 306 |
| MseI | TTAA | 2 cut(s) | 441, 533 |
| MslI | CAYNNNNRTG | 1 cut(s) | 63 |
| Msp20I | TGGCCA | 1 cut(s) | 306 |
| MspA1I | CMGCKG | 2 cut(s) | 279, 678 |
| MspR9I | CCNGG | 1 cut(s) | 458 |
| MunI | CAATTG | 2 cut(s) | 732, 873 |
| MvaI | CCWGG | 1 cut(s) | 458 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 358, 633 |
| NdeII | GATC | 3 cut(s) | 172, 292, 403 |
| NlaIII | CATG | 9 cut(s) | 65, 410, 452, 511, 579, 714, 730, 772, 832 |
| NmuCI | GTSAC | 1 cut(s) | 423 |
| NsiI | ATGCAT | 1 cut(s) | 577 |
| NspI | RCATGY | 2 cut(s) | 511, 832 |
| PagI | TCATGA | 2 cut(s) | 61, 768 |
| PflFI | GACNNNGTC | 1 cut(s) | 815 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 302 |
| PkrI | GCNGC | 5 cut(s) | 38, 281, 524, 638, 800 |
| PsiI | TTATAA | 1 cut(s) | 516 |
| Psp6I | CCWGG | 1 cut(s) | 456 |
| PspGI | CCWGG | 1 cut(s) | 456 |
| PsyI | GACNNNGTC | 1 cut(s) | 815 |
| PvuII | CAGCTG | 2 cut(s) | 279, 678 |
| RsaI | GTAC | 6 cut(s) | 54, 207, 462, 542, 617, 696 |
| RsaNI | GTAC | 6 cut(s) | 53, 206, 461, 541, 616, 695 |
| RseI | CAYNNNNRTG | 1 cut(s) | 63 |
| SaqAI | TTAA | 2 cut(s) | 441, 533 |
| SatI | GCNGC | 5 cut(s) | 37, 280, 523, 637, 799 |
| Sau3AI | GATC | 3 cut(s) | 172, 292, 403 |
| ScaI | AGTACT | 1 cut(s) | 207 |
| ScrFI | CCNGG | 1 cut(s) | 458 |
| SduI | GDGCHC | 2 cut(s) | 269, 768 |
| SexAI | ACCWGGT | 1 cut(s) | 456 |
| SfaNI | GCATC | 2 cut(s) | 48, 169 |
| SfcI | CTRYAG | 1 cut(s) | 679 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 63 |
| SmlI | CTYRAG | 2 cut(s) | 226, 647 |
| SmoI | CTYRAG | 2 cut(s) | 226, 647 |
| Sse9I | AATT | 6 cut(s) | 183, 190, 329, 732, 861, 873 |
| SsiI | CCGC | 2 cut(s) | 352, 637 |
| SspMI | CTAG | 2 cut(s) | 209, 222 |
| StyD4I | CCNGG | 1 cut(s) | 456 |
| TaaI | ACNGT | 7 cut(s) | 16, 439, 540, 605, 760, 789, 850 |
| TaiI | ACGT | 1 cut(s) | 696 |
| TaqI | TCGA | 2 cut(s) | 171, 263 |
| TasI | AATT | 6 cut(s) | 183, 190, 329, 732, 861, 873 |
| TatI | WGTACW | 2 cut(s) | 205, 540 |
| TauI | GCSGC | 1 cut(s) | 639 |
| Tru1I | TTAA | 2 cut(s) | 441, 533 |
| Tru9I | TTAA | 2 cut(s) | 441, 533 |
| TseFI | GTSAC | 1 cut(s) | 423 |
| TseI | GCWGC | 4 cut(s) | 36, 279, 522, 798 |
| Tsp45I | GTSAC | 1 cut(s) | 423 |
| TspDTI | ATGAA | 1 cut(s) | 465 |
| Tth111I | GACNNNGTC | 1 cut(s) | 815 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 302 |
| XapI | RAATTY | 2 cut(s) | 329, 861 |
| XceI | RCATGY | 2 cut(s) | 511, 832 |
| XmiI | GTMKAC | 1 cut(s) | 723 |
| XspI | CTAG | 2 cut(s) | 209, 222 |
| ZrmI | AGTACT | 1 cut(s) | 207 |
| Zsp2I | ATGCAT | 1 cut(s) | 577 |