DNA recombination
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 246 |
| AciI | CCGC | 1 cut(s) | 334 |
| AclWI | GGATC | 1 cut(s) | 35 |
| AcsI | RAATTY | 1 cut(s) | 183 |
| AcuI | CTGAAG | 1 cut(s) | 22 |
| AfaI | GTAC | 1 cut(s) | 20 |
| AgsI | TTSAA | 5 cut(s) | 75, 146, 189, 272, 322 |
| AluBI | AGCT | 2 cut(s) | 36, 280 |
| AluI | AGCT | 2 cut(s) | 36, 280 |
| AlwI | GGATC | 1 cut(s) | 35 |
| Ama87I | CYCGRG | 1 cut(s) | 56 |
| AoxI | GGCC | 1 cut(s) | 6 |
| ApeKI | GCWGC | 1 cut(s) | 33 |
| ApoI | RAATTY | 1 cut(s) | 183 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 317 |
| AspLEI | GCGC | 1 cut(s) | 246 |
| AsuHPI | GGTGA | 1 cut(s) | 375 |
| AsuII | TTCGAA | 1 cut(s) | 129 |
| AsuNHI | GCTAGC | 1 cut(s) | 236 |
| AvaI | CYCGRG | 1 cut(s) | 56 |
| BaeGI | GKGCMC | 1 cut(s) | 156 |
| BauI | CACGAG | 1 cut(s) | 356 |
| BbsI | GAAGAC | 1 cut(s) | 369 |
| BbvI | GCAGC | 1 cut(s) | 45 |
| BccI | CCATC | 1 cut(s) | 227 |
| BfaI | CTAG | 1 cut(s) | 237 |
| BisI | GCNGC | 1 cut(s) | 34 |
| BlsI | GCNGC | 1 cut(s) | 35 |
| BmeT110I | CYCGRG | 1 cut(s) | 56 |
| BmsI | GCATC | 2 cut(s) | 52, 223 |
| BmtI | GCTAGC | 1 cut(s) | 240 |
| BpiI | GAAGAC | 1 cut(s) | 369 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 157, 189 |
| BpmI | CTGGAG | 1 cut(s) | 159 |
| Bpu14I | TTCGAA | 1 cut(s) | 129 |
| Bse1I | ACTGG | 1 cut(s) | 26 |
| Bse3DI | GCAATG | 1 cut(s) | 19 |
| BseGI | GGATG | 2 cut(s) | 67, 211 |
| BseMI | GCAATG | 1 cut(s) | 19 |
| BseMII | CTCAG | 1 cut(s) | 320 |
| BseNI | ACTGG | 1 cut(s) | 26 |
| BseSI | GKGCMC | 1 cut(s) | 156 |
| BseXI | GCAGC | 1 cut(s) | 45 |
| Bsh1236I | CGCG | 1 cut(s) | 246 |
| BshFI | GGCC | 1 cut(s) | 8 |
| BsiHKCI | CYCGRG | 1 cut(s) | 56 |
| BsnI | GGCC | 1 cut(s) | 8 |
| BsoBI | CYCGRG | 1 cut(s) | 56 |
| Bsp119I | TTCGAA | 1 cut(s) | 129 |
| Bsp1286I | GDGCHC | 1 cut(s) | 156 |
| Bsp143I | GATC | 2 cut(s) | 27, 191 |
| BspACI | CCGC | 1 cut(s) | 334 |
| BspANI | GGCC | 1 cut(s) | 8 |
| BspCNI | CTCAG | 1 cut(s) | 319 |
| BspFNI | CGCG | 1 cut(s) | 246 |
| BspOI | GCTAGC | 1 cut(s) | 240 |
| BspPI | GGATC | 1 cut(s) | 35 |
| BspT104I | TTCGAA | 1 cut(s) | 129 |
| BsrDI | GCAATG | 1 cut(s) | 19 |
| BsrI | ACTGG | 1 cut(s) | 26 |
| BssMI | GATC | 2 cut(s) | 27, 191 |
| BssSI | CACGAG | 1 cut(s) | 356 |
| Bst2BI | CACGAG | 1 cut(s) | 356 |
| Bst6I | CTCTTC | 1 cut(s) | 323 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 233 |
| BstBI | TTCGAA | 1 cut(s) | 129 |
| BstC8I | GCNNGC | 4 cut(s) | 10, 67, 238, 242 |
| BstDEI | CTNAG | 1 cut(s) | 306 |
| BstF5I | GGATG | 2 cut(s) | 67, 211 |
| BstFNI | CGCG | 1 cut(s) | 246 |
| BstHHI | GCGC | 1 cut(s) | 246 |
| BstKTI | GATC | 2 cut(s) | 30, 194 |
| BstMBI | GATC | 2 cut(s) | 27, 191 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 160, 233 |
| BstSLI | GKGCMC | 1 cut(s) | 156 |
| BstUI | CGCG | 1 cut(s) | 246 |
| BstV1I | GCAGC | 1 cut(s) | 45 |
| BstV2I | GAAGAC | 1 cut(s) | 369 |
| BsuRI | GGCC | 1 cut(s) | 8 |
| BtsCI | GGATG | 2 cut(s) | 67, 211 |
| BtsI | GCAGTG | 1 cut(s) | 222 |
| BtsIMutI | CAGTG | 1 cut(s) | 222 |
| Cac8I | GCNNGC | 4 cut(s) | 10, 67, 238, 242 |
| CfoI | GCGC | 1 cut(s) | 246 |
| Csp6I | GTAC | 1 cut(s) | 19 |
| CviAII | CATG | 1 cut(s) | 85 |
| CviJI | RGCY | 5 cut(s) | 8, 36, 83, 264, 280 |
| CviKI_1 | RGCY | 5 cut(s) | 8, 36, 83, 264, 280 |
| CviQI | GTAC | 1 cut(s) | 19 |
| DdeI | CTNAG | 1 cut(s) | 306 |
| DpnI | GATC | 2 cut(s) | 29, 193 |
| DpnII | GATC | 2 cut(s) | 27, 191 |
| Eam1104I | CTCTTC | 1 cut(s) | 323 |
| EarI | CTCTTC | 1 cut(s) | 323 |
| Eco57I | CTGAAG | 1 cut(s) | 22 |
| Eco88I | CYCGRG | 1 cut(s) | 56 |
| FaeI | CATG | 1 cut(s) | 88 |
| FaiI | YATR | 1 cut(s) | 86 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 302, 334 |
| FatI | CATG | 1 cut(s) | 84 |
| FauI | CCCGC | 1 cut(s) | 341 |
| Fnu4HI | GCNGC | 1 cut(s) | 34 |
| FokI | GGATG | 2 cut(s) | 74, 198 |
| Fsp4HI | GCNGC | 1 cut(s) | 34 |
| FspBI | CTAG | 1 cut(s) | 237 |
| GlaI | GCGC | 1 cut(s) | 245 |
| GluI | GCNGC | 1 cut(s) | 34 |
| GsuI | CTGGAG | 1 cut(s) | 159 |
| HaeIII | GGCC | 1 cut(s) | 8 |
| HhaI | GCGC | 1 cut(s) | 246 |
| Hin1II | CATG | 1 cut(s) | 88 |
| Hin6I | GCGC | 1 cut(s) | 244 |
| HinP1I | GCGC | 1 cut(s) | 244 |
| HinfI | GANTC | 3 cut(s) | 71, 120, 298 |
| HphI | GGTGA | 1 cut(s) | 375 |
| Hpy166II | GTNNAC | 1 cut(s) | 19 |
| Hpy188I | TCNGA | 3 cut(s) | 41, 309, 328 |
| Hpy188III | TCNNGA | 4 cut(s) | 56, 176, 189, 207 |
| Hpy8I | GTNNAC | 1 cut(s) | 19 |
| HpyAV | CCTTC | 2 cut(s) | 143, 278 |
| HpyCH4IV | ACGT | 1 cut(s) | 258 |
| HpyCH4V | TGCA | 4 cut(s) | 12, 65, 227, 285 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 160, 233 |
| HpyF3I | CTNAG | 1 cut(s) | 306 |
| HpySE526I | ACGT | 1 cut(s) | 258 |
| Hsp92II | CATG | 1 cut(s) | 88 |
| HspAI | GCGC | 1 cut(s) | 244 |
| Kzo9I | GATC | 2 cut(s) | 27, 191 |
| LpnPI | CCDG | 7 cut(s) | 7, 22, 114, 189, 220, 266, 374 |
| Lsp1109I | GCAGC | 1 cut(s) | 45 |
| LweI | GCATC | 2 cut(s) | 52, 223 |
| MaeI | CTAG | 1 cut(s) | 237 |
| MaeII | ACGT | 1 cut(s) | 258 |
| MalI | GATC | 2 cut(s) | 29, 193 |
| MboI | GATC | 2 cut(s) | 27, 191 |
| MboII | GAAGA | 4 cut(s) | 158, 310, 322, 374 |
| MhlI | GDGCHC | 1 cut(s) | 156 |
| MluCI | AATT | 1 cut(s) | 183 |
| MlyI | GAGTC | 2 cut(s) | 129, 292 |
| MmeI | TCCRAC | 1 cut(s) | 239 |
| MnlI | CCTC | 4 cut(s) | 40, 52, 183, 352 |
| MroXI | GAANNNNTTC | 1 cut(s) | 317 |
| MseI | TTAA | 2 cut(s) | 293, 409 |
| MvnI | CGCG | 1 cut(s) | 246 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 160, 233 |
| NdeII | GATC | 2 cut(s) | 27, 191 |
| NheI | GCTAGC | 1 cut(s) | 236 |
| NlaIII | CATG | 1 cut(s) | 88 |
| NspV | TTCGAA | 1 cut(s) | 129 |
| PaeR7I | CTCGAG | 1 cut(s) | 56 |
| PdmI | GAANNNNTTC | 1 cut(s) | 317 |
| PfeI | GAWTC | 1 cut(s) | 71 |
| PkrI | GCNGC | 1 cut(s) | 35 |
| PleI | GAGTC | 2 cut(s) | 128, 292 |
| PpsI | GAGTC | 2 cut(s) | 128, 292 |
| RsaI | GTAC | 1 cut(s) | 20 |
| RsaNI | GTAC | 1 cut(s) | 19 |
| SaqAI | TTAA | 2 cut(s) | 293, 409 |
| SatI | GCNGC | 1 cut(s) | 34 |
| Sau3AI | GATC | 2 cut(s) | 27, 191 |
| SchI | GAGTC | 2 cut(s) | 129, 292 |
| SduI | GDGCHC | 1 cut(s) | 156 |
| SetI | ASST | 6 cut(s) | 38, 51, 261, 270, 282, 393 |
| SfaNI | GCATC | 2 cut(s) | 52, 223 |
| Sfr274I | CTCGAG | 1 cut(s) | 56 |
| SfuI | TTCGAA | 1 cut(s) | 129 |
| SlaI | CTCGAG | 1 cut(s) | 56 |
| SmlI | CTYRAG | 1 cut(s) | 56 |
| SmoI | CTYRAG | 1 cut(s) | 56 |
| Sse9I | AATT | 1 cut(s) | 183 |
| SsiI | CCGC | 1 cut(s) | 334 |
| SspMI | CTAG | 1 cut(s) | 237 |
| TaiI | ACGT | 1 cut(s) | 261 |
| TaqI | TCGA | 2 cut(s) | 57, 129 |
| TasI | AATT | 1 cut(s) | 183 |
| TatI | WGTACW | 1 cut(s) | 18 |
| TfiI | GAWTC | 1 cut(s) | 71 |
| Tru1I | TTAA | 2 cut(s) | 293, 409 |
| Tru9I | TTAA | 2 cut(s) | 293, 409 |
| TscAI | CASTG | 1 cut(s) | 229 |
| TseI | GCWGC | 1 cut(s) | 33 |
| TspRI | CASTG | 1 cut(s) | 229 |
| XapI | RAATTY | 1 cut(s) | 183 |
| XhoI | CTCGAG | 1 cut(s) | 56 |
| XmnI | GAANNNNTTC | 1 cut(s) | 317 |
| XspI | CTAG | 1 cut(s) | 237 |