Belongs to the helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 65 |
| BpuEI | CTTGAG | 1 cut(s) | 155 |
| Bse1I | ACTGG | 1 cut(s) | 120 |
| BseNI | ACTGG | 1 cut(s) | 120 |
| BseYI | CCCAGC | 1 cut(s) | 145 |
| BslFI | GGGAC | 1 cut(s) | 125 |
| BsmFI | GGGAC | 1 cut(s) | 125 |
| BsrI | ACTGG | 1 cut(s) | 120 |
| BstNSI | RCATGY | 1 cut(s) | 192 |
| BtsI | GCAGTG | 2 cut(s) | 9, 99 |
| BtsIMutI | CAGTG | 2 cut(s) | 9, 99 |
| CviAII | CATG | 1 cut(s) | 189 |
| CviJI | RGCY | 1 cut(s) | 168 |
| CviKI_1 | RGCY | 1 cut(s) | 168 |
| FaeI | CATG | 1 cut(s) | 192 |
| FaiI | YATR | 5 cut(s) | 42, 48, 53, 92, 190 |
| FaqI | GGGAC | 1 cut(s) | 125 |
| FatI | CATG | 1 cut(s) | 188 |
| GsaI | CCCAGC | 1 cut(s) | 149 |
| Hin1II | CATG | 1 cut(s) | 192 |
| HinfI | GANTC | 1 cut(s) | 61 |
| Hpy188III | TCNNGA | 1 cut(s) | 65 |
| HpyCH4V | TGCA | 3 cut(s) | 14, 104, 130 |
| Hsp92II | CATG | 1 cut(s) | 192 |
| LpnPI | CCDG | 2 cut(s) | 133, 159 |
| MluCI | AATT | 3 cut(s) | 82, 134, 182 |
| MnlI | CCTC | 1 cut(s) | 179 |
| MseI | TTAA | 1 cut(s) | 177 |
| MslI | CAYNNNNRTG | 1 cut(s) | 193 |
| NlaIII | CATG | 1 cut(s) | 192 |
| NspI | RCATGY | 1 cut(s) | 192 |
| PfeI | GAWTC | 1 cut(s) | 61 |
| PspFI | CCCAGC | 1 cut(s) | 145 |
| RseI | CAYNNNNRTG | 1 cut(s) | 193 |
| SaqAI | TTAA | 1 cut(s) | 177 |
| SetI | ASST | 1 cut(s) | 177 |
| SgeI | CNNG | 6 cut(s) | 45, 77, 132, 158, 177, 184 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 193 |
| SmlI | CTYRAG | 1 cut(s) | 170 |
| SmoI | CTYRAG | 1 cut(s) | 170 |
| Sse9I | AATT | 3 cut(s) | 82, 134, 182 |
| SspI | AATATT | 1 cut(s) | 57 |
| TaqI | TCGA | 1 cut(s) | 32 |
| TasI | AATT | 3 cut(s) | 82, 134, 182 |
| TfiI | GAWTC | 1 cut(s) | 61 |
| Tru1I | TTAA | 1 cut(s) | 177 |
| Tru9I | TTAA | 1 cut(s) | 177 |
| TscAI | CASTG | 2 cut(s) | 16, 106 |
| TspDTI | ATGAA | 1 cut(s) | 13 |
| TspRI | CASTG | 2 cut(s) | 16, 106 |
| XceI | RCATGY | 1 cut(s) | 192 |