DNA recombination
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 91 |
| AciI | CCGC | 3 cut(s) | 179, 330, 539 |
| AclWI | GGATC | 3 cut(s) | 83, 96, 213 |
| AcoI | YGGCCR | 1 cut(s) | 217 |
| AcsI | RAATTY | 2 cut(s) | 39, 680 |
| AdeI | CACNNNGTG | 1 cut(s) | 505 |
| AfaI | GTAC | 3 cut(s) | 167, 266, 492 |
| AjnI | CCWGG | 1 cut(s) | 363 |
| AluBI | AGCT | 4 cut(s) | 35, 223, 397, 643 |
| AluI | AGCT | 4 cut(s) | 35, 223, 397, 643 |
| Alw21I | GWGCWC | 1 cut(s) | 645 |
| Alw26I | GTCTC | 1 cut(s) | 373 |
| AlwI | GGATC | 3 cut(s) | 83, 96, 213 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 382 |
| Aor13HI | TCCGGA | 1 cut(s) | 91 |
| AoxI | GGCC | 1 cut(s) | 217 |
| ApeKI | GCWGC | 3 cut(s) | 394, 526, 541 |
| ApoI | RAATTY | 2 cut(s) | 39, 680 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 137, 169 |
| AspS9I | GGNCC | 1 cut(s) | 388 |
| AsuHPI | GGTGA | 1 cut(s) | 588 |
| AsuII | TTCGAA | 1 cut(s) | 17 |
| AvaII | GGWCC | 1 cut(s) | 388 |
| BalI | TGGCCA | 1 cut(s) | 219 |
| BamHI | GGATCC | 1 cut(s) | 88 |
| BanII | GRGCYC | 1 cut(s) | 645 |
| Bbv12I | GWGCWC | 1 cut(s) | 645 |
| BbvI | GCAGC | 3 cut(s) | 406, 538, 553 |
| BccI | CCATC | 1 cut(s) | 649 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 188, 222 |
| BciT130I | CCWGG | 1 cut(s) | 365 |
| BcoDI | GTCTC | 1 cut(s) | 373 |
| BisI | GCNGC | 4 cut(s) | 331, 395, 527, 542 |
| BlsI | GCNGC | 4 cut(s) | 332, 396, 528, 543 |
| Bme1390I | CCNGG | 1 cut(s) | 365 |
| Bme18I | GGWCC | 1 cut(s) | 388 |
| BmgT120I | GGNCC | 1 cut(s) | 388 |
| BmiI | GGNNCC | 2 cut(s) | 90, 672 |
| BmrFI | CCNGG | 1 cut(s) | 365 |
| BmsI | GCATC | 2 cut(s) | 137, 186 |
| Bpu14I | TTCGAA | 1 cut(s) | 17 |
| BsaJI | CCNNGG | 2 cut(s) | 260, 674 |
| BsaWI | WCCGGW | 1 cut(s) | 91 |
| Bse1I | ACTGG | 3 cut(s) | 213, 387, 530 |
| Bse3DI | GCAATG | 1 cut(s) | 188 |
| BseAI | TCCGGA | 1 cut(s) | 91 |
| BseBI | CCWGG | 1 cut(s) | 365 |
| BseDI | CCNNGG | 2 cut(s) | 260, 674 |
| BseGI | GGATG | 2 cut(s) | 112, 238 |
| BseMI | GCAATG | 1 cut(s) | 188 |
| BseMII | CTCAG | 3 cut(s) | 430, 501, 616 |
| BseNI | ACTGG | 3 cut(s) | 213, 387, 530 |
| BseXI | GCAGC | 3 cut(s) | 406, 538, 553 |
| BshFI | GGCC | 1 cut(s) | 219 |
| BsiHKAI | GWGCWC | 1 cut(s) | 645 |
| BsiSI | CCGG | 1 cut(s) | 92 |
| BsmAI | GTCTC | 1 cut(s) | 373 |
| BsmI | GAATGC | 1 cut(s) | 313 |
| BsnI | GGCC | 1 cut(s) | 219 |
| Bsp119I | TTCGAA | 1 cut(s) | 17 |
| Bsp1286I | GDGCHC | 1 cut(s) | 645 |
| Bsp13I | TCCGGA | 1 cut(s) | 91 |
| Bsp143I | GATC | 2 cut(s) | 88, 205 |
| BspACI | CCGC | 3 cut(s) | 179, 330, 539 |
| BspANI | GGCC | 1 cut(s) | 219 |
| BspCNI | CTCAG | 3 cut(s) | 429, 502, 615 |
| BspEI | TCCGGA | 1 cut(s) | 91 |
| BspLI | GGNNCC | 2 cut(s) | 90, 672 |
| BspPI | GGATC | 3 cut(s) | 83, 96, 213 |
| BspT104I | TTCGAA | 1 cut(s) | 17 |
| BsrDI | GCAATG | 1 cut(s) | 188 |
| BsrI | ACTGG | 3 cut(s) | 213, 387, 530 |
| BssECI | CCNNGG | 2 cut(s) | 260, 674 |
| BssMI | GATC | 2 cut(s) | 88, 205 |
| BssT1I | CCWWGG | 1 cut(s) | 260 |
| Bst2UI | CCWGG | 1 cut(s) | 365 |
| Bst4CI | ACNGT | 3 cut(s) | 170, 565, 580 |
| Bst6I | CTCTTC | 2 cut(s) | 19, 616 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 354 |
| BstBI | TTCGAA | 1 cut(s) | 17 |
| BstC8I | GCNNGC | 4 cut(s) | 175, 179, 221, 350 |
| BstDEI | CTNAG | 3 cut(s) | 416, 510, 602 |
| BstF5I | GGATG | 2 cut(s) | 112, 238 |
| BstKTI | GATC | 2 cut(s) | 91, 208 |
| BstMAI | GTCTC | 1 cut(s) | 373 |
| BstMBI | GATC | 2 cut(s) | 88, 205 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 32, 354 |
| BstNI | CCWGG | 1 cut(s) | 365 |
| BstNSI | RCATGY | 1 cut(s) | 352 |
| BstSCI | CCNGG | 1 cut(s) | 363 |
| BstV1I | GCAGC | 3 cut(s) | 406, 538, 553 |
| BstX2I | RGATCY | 1 cut(s) | 88 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 261 |
| BstYI | RGATCY | 1 cut(s) | 88 |
| BsuRI | GGCC | 1 cut(s) | 219 |
| BtsCI | GGATG | 2 cut(s) | 112, 238 |
| BtsIMutI | CAGTG | 4 cut(s) | 175, 220, 570, 576 |
| Cac8I | GCNNGC | 4 cut(s) | 175, 179, 221, 350 |
| CaiI | CAGNNNCTG | 1 cut(s) | 382 |
| Cfr13I | GGNCC | 1 cut(s) | 388 |
| CsiI | ACCWGGT | 1 cut(s) | 363 |
| Csp6I | GTAC | 3 cut(s) | 166, 265, 491 |
| CviAII | CATG | 1 cut(s) | 349 |
| CviJI | RGCY | 9 cut(s) | 35, 219, 223, 397, 421, 529, 544, 643, 673 |
| CviKI_1 | RGCY | 9 cut(s) | 35, 219, 223, 397, 421, 529, 544, 643, 673 |
| CviQI | GTAC | 3 cut(s) | 166, 265, 491 |
| DdeI | CTNAG | 3 cut(s) | 416, 510, 602 |
| DpnI | GATC | 2 cut(s) | 90, 207 |
| DpnII | GATC | 2 cut(s) | 88, 205 |
| DraIII | CACNNNGTG | 1 cut(s) | 505 |
| EaeI | YGGCCR | 1 cut(s) | 217 |
| Eam1104I | CTCTTC | 2 cut(s) | 19, 616 |
| EarI | CTCTTC | 2 cut(s) | 19, 616 |
| Ecl136II | GAGCTC | 1 cut(s) | 643 |
| Eco130I | CCWWGG | 1 cut(s) | 260 |
| Eco24I | GRGCYC | 1 cut(s) | 645 |
| Eco47I | GGWCC | 1 cut(s) | 388 |
| Eco53kI | GAGCTC | 1 cut(s) | 643 |
| EcoICRI | GAGCTC | 1 cut(s) | 643 |
| EcoRII | CCWGG | 1 cut(s) | 363 |
| EcoT14I | CCWWGG | 1 cut(s) | 260 |
| EcoT38I | GRGCYC | 1 cut(s) | 645 |
| ErhI | CCWWGG | 1 cut(s) | 260 |
| FaeI | CATG | 1 cut(s) | 352 |
| FaiI | YATR | 6 cut(s) | 350, 516, 524, 612, 614, 691 |
| FatI | CATG | 1 cut(s) | 348 |
| FauI | CCCGC | 1 cut(s) | 186 |
| Fnu4HI | GCNGC | 4 cut(s) | 331, 395, 527, 542 |
| FokI | GGATG | 2 cut(s) | 119, 225 |
| FriOI | GRGCYC | 1 cut(s) | 645 |
| Fsp4HI | GCNGC | 4 cut(s) | 331, 395, 527, 542 |
| GluI | GCNGC | 4 cut(s) | 331, 395, 527, 542 |
| HaeIII | GGCC | 1 cut(s) | 219 |
| HapII | CCGG | 1 cut(s) | 92 |
| Hin1II | CATG | 1 cut(s) | 352 |
| HincII | GTYRAC | 1 cut(s) | 552 |
| HindII | GTYRAC | 1 cut(s) | 552 |
| HinfI | GANTC | 3 cut(s) | 250, 373, 628 |
| HpaII | CCGG | 1 cut(s) | 92 |
| HphI | GGTGA | 1 cut(s) | 588 |
| Hpy166II | GTNNAC | 2 cut(s) | 388, 552 |
| Hpy188I | TCNGA | 2 cut(s) | 378, 687 |
| Hpy188III | TCNNGA | 1 cut(s) | 92 |
| Hpy8I | GTNNAC | 2 cut(s) | 388, 552 |
| HpyAV | CCTTC | 1 cut(s) | 430 |
| HpyCH4III | ACNGT | 3 cut(s) | 170, 565, 580 |
| HpyCH4V | TGCA | 6 cut(s) | 46, 100, 123, 199, 357, 526 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 32, 354 |
| HpyF3I | CTNAG | 3 cut(s) | 416, 510, 602 |
| Hsp92II | CATG | 1 cut(s) | 352 |
| Kpn2I | TCCGGA | 1 cut(s) | 91 |
| Kzo9I | GATC | 2 cut(s) | 88, 205 |
| LmnI | GCTCC | 2 cut(s) | 9, 670 |
| Lsp1109I | GCAGC | 3 cut(s) | 406, 538, 553 |
| LweI | GCATC | 2 cut(s) | 137, 186 |
| MabI | ACCWGGT | 1 cut(s) | 363 |
| MaeIII | GTNAC | 3 cut(s) | 61, 505, 532 |
| MalI | GATC | 2 cut(s) | 90, 207 |
| MboI | GATC | 2 cut(s) | 88, 205 |
| MboII | GAAGA | 4 cut(s) | 6, 299, 579, 633 |
| MflI | RGATCY | 1 cut(s) | 88 |
| MhlI | GDGCHC | 1 cut(s) | 645 |
| MlsI | TGGCCA | 1 cut(s) | 219 |
| MluCI | AATT | 5 cut(s) | 39, 74, 325, 632, 680 |
| MluNI | TGGCCA | 1 cut(s) | 219 |
| MmeI | TCCRAC | 1 cut(s) | 278 |
| MnlI | CCTC | 6 cut(s) | 18, 22, 459, 580, 617, 669 |
| Mox20I | TGGCCA | 1 cut(s) | 219 |
| MroI | TCCGGA | 1 cut(s) | 91 |
| MscI | TGGCCA | 1 cut(s) | 219 |
| MseI | TTAA | 3 cut(s) | 135, 273, 477 |
| MslI | CAYNNNNRTG | 2 cut(s) | 105, 118 |
| Msp20I | TGGCCA | 1 cut(s) | 219 |
| MspI | CCGG | 1 cut(s) | 92 |
| MspR9I | CCNGG | 1 cut(s) | 365 |
| Mva1269I | GAATGC | 1 cut(s) | 313 |
| MvaI | CCWGG | 1 cut(s) | 365 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 32, 354 |
| NdeII | GATC | 2 cut(s) | 88, 205 |
| NlaIII | CATG | 1 cut(s) | 352 |
| NlaIV | GGNNCC | 2 cut(s) | 90, 672 |
| NmuCI | GTSAC | 1 cut(s) | 505 |
| NspI | RCATGY | 1 cut(s) | 352 |
| NspV | TTCGAA | 1 cut(s) | 17 |
| PaeI | GCATGC | 1 cut(s) | 352 |
| PctI | GAATGC | 1 cut(s) | 313 |
| PfeI | GAWTC | 3 cut(s) | 250, 373, 628 |
| PkrI | GCNGC | 4 cut(s) | 332, 396, 528, 543 |
| Psp124BI | GAGCTC | 1 cut(s) | 645 |
| Psp6I | CCWGG | 1 cut(s) | 363 |
| PspGI | CCWGG | 1 cut(s) | 363 |
| PspN4I | GGNNCC | 2 cut(s) | 90, 672 |
| PspPI | GGNCC | 1 cut(s) | 388 |
| PstNI | CAGNNNCTG | 1 cut(s) | 382 |
| PsuI | RGATCY | 1 cut(s) | 88 |
| RsaI | GTAC | 3 cut(s) | 167, 266, 492 |
| RsaNI | GTAC | 3 cut(s) | 166, 265, 491 |
| RseI | CAYNNNNRTG | 2 cut(s) | 105, 118 |
| SacI | GAGCTC | 1 cut(s) | 645 |
| SaqAI | TTAA | 3 cut(s) | 135, 273, 477 |
| SatI | GCNGC | 4 cut(s) | 331, 395, 527, 542 |
| Sau3AI | GATC | 2 cut(s) | 88, 205 |
| Sau96I | GGNCC | 1 cut(s) | 388 |
| ScrFI | CCNGG | 1 cut(s) | 365 |
| SduI | GDGCHC | 1 cut(s) | 645 |
| SetI | ASST | 6 cut(s) | 37, 225, 366, 399, 441, 645 |
| SexAI | ACCWGGT | 1 cut(s) | 363 |
| SfaNI | GCATC | 2 cut(s) | 137, 186 |
| SfuI | TTCGAA | 1 cut(s) | 17 |
| SinI | GGWCC | 1 cut(s) | 388 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 105, 118 |
| SphI | GCATGC | 1 cut(s) | 352 |
| Sse9I | AATT | 5 cut(s) | 39, 74, 325, 632, 680 |
| SsiI | CCGC | 3 cut(s) | 179, 330, 539 |
| SstI | GAGCTC | 1 cut(s) | 645 |
| StyD4I | CCNGG | 1 cut(s) | 363 |
| StyI | CCWWGG | 1 cut(s) | 260 |
| TaaI | ACNGT | 3 cut(s) | 170, 565, 580 |
| TaqI | TCGA | 4 cut(s) | 17, 156, 208, 285 |
| TasI | AATT | 5 cut(s) | 39, 74, 325, 632, 680 |
| TatI | WGTACW | 1 cut(s) | 490 |
| TauI | GCSGC | 1 cut(s) | 333 |
| TfiI | GAWTC | 3 cut(s) | 250, 373, 628 |
| Tru1I | TTAA | 3 cut(s) | 135, 273, 477 |
| Tru9I | TTAA | 3 cut(s) | 135, 273, 477 |
| TscAI | CASTG | 4 cut(s) | 175, 220, 570, 583 |
| TseFI | GTSAC | 1 cut(s) | 505 |
| TseI | GCWGC | 3 cut(s) | 394, 526, 541 |
| Tsp45I | GTSAC | 1 cut(s) | 505 |
| TspDTI | ATGAA | 6 cut(s) | 87, 306, 315, 320, 386, 573 |
| TspGWI | ACGGA | 1 cut(s) | 633 |
| TspRI | CASTG | 4 cut(s) | 175, 220, 570, 583 |
| VpaK11BI | GGWCC | 1 cut(s) | 388 |
| XapI | RAATTY | 2 cut(s) | 39, 680 |
| XceI | RCATGY | 1 cut(s) | 352 |