Rorug02G0145400

DNA recombination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000050
Physical Location & Seq
Reverse (-)
12759892 .. 12764785
4894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0145400.1

Sequence Viewer

Length: 2904 bp
ATGGTTCACAGTGCCTACGATTCCGTCGAGCTCATCTCCGACTGCCCCACCAAGATCGAAGCCATCGAATCCTACGGCCCTAAGCTTCTCCTCGGCTGCTCCGATGGATCCCTCCGAATCTACGCTCCAGATTCCTCCGGCTCCCGCTCCCCACCCTCCGACTACCACACTCAATCCCTCCAAAAGGAACCCTACTCCCTCGAGAGAAACATCACCGGGTTCTCTAAGAAGCCGCTGCTCTCTATGGAGGTCTTGGAATCCAGAGAGCTCCTTTTGTCCCTCTCCGAAACGATATCGTTTCACGGCCTCCCGAATTTGGGCACCATCGCCGTGATCACCAAGGCCAAGGGCGCCAATGTGTACTCCTGGGACGACCGCCGCGGATTCCTGTGCTTCGCCAGGCAGAAGAAAGTCTGTATTTTCCGACACGACGGTGGAAGAGGTTTCGTGGAGGTGAAGGAATTCGGAGTTCCGGATGTGGTGAAGTCTATGTCATGGTGTGGAGAGAATATATGTATAGGGATTAGGAGAGATTATATGATACTCAATTCTACAACTGGTGCATTGACTGATGTGTTTCCTTCGGGGAGGCTGGCTCCGCCGTTGGTGGTTCCTCTTCCTTCCGGGGAGCTTCTTCTTGGGAAGGATAACATTGGGGTTTTTGTGGACCAAAATGGGAAACTTGCTCATGAAGGCAGGGTTTGTTGGTCAGAGGCGCCTACGGTTGTTGTTATTCAGAAGGCCTATGGAATAGCTCTGTTATCGAGATATGTTGAGGTTCGGTCCCTACGAGCGCCATATCCATTGATACAAACGGTTGTTCTTCGAAATGCTCGTCGTCTTCTCCAAAGCAACAATGCTGCTATTGTTGCATTAGACTATGCTGTTTATGGTCTCTTCCCTGTCCCTCTTGGTGCCCAGATTGTACAATTAACAGCATCGGGTGAGTTTGAGGAAGCTTTGTCATTATGTAAGCTGCTTCCGCCTGAAGAAGCAAGCCTTAGAGCTGCAAAGGAGGCTTCAATCCATATAAGATGCGCTCACCATCGTTTTGATACTGGGGACTATGAGGATGCCATGGAGCATTTTGTGGCATCTCAAGTAGATATAACCTATGTACTTTCTATGTATCCTTCTATTGTTCTTCCTAAGACAACCATGATATCTGATCCAGACAAACTGATGGATATATCTGGGGACACATCATATCTCTCAAGAGGTTCATCTGGTATGTCAGATGATATGGAACCCTCTGCACTGTCACATGTGCTGGAATCAGAGGAGAATGCAGCATTTGAGTCCAAAAAAATGAGCCATAACACTCTCATGGCTCTCATTAAGTTCTTGCAGAAAAAGAGATTCAGCATAATTGAAAAAGCTACTGCTGAGGGGACAGAAGAGGTTGTTTTGGATGCTGTTGGAGACAAATTCGCATCCCATGAGTCTAATAGGTATAAGAAGACAAACAAGGGGCGTGGCAGCATTCCAGTTACCTCCCGTGCTAGGGAGATGGCAGCTATACTGGATACAGCCCTACTTCAAGCTCTACTTCTTACTGGACAGTCTTCAGCGGCTATGGAATTACTGAAAGGTCGTAATTACTGTGATGTAAAAATATGCGAGGAGATTCTCCTAAAAAGCAATCATCATGCTGCTTTGTTAGAACTTTACAAGTGCAATTCAATGCACCATGAAGCTCTCAAACTTTTGCATCAGTTAGTAGAAGAGTCAAAATCTAATCAAGTGCCACCTGAGGTGATCCAAAAGATAAAGCCGGAGTCAATTGTTGAATATCTCAAGCCTCTTTGTGGCACTGATCCCATGCTCGTCTTGGAGTATTCAATGCTTGTCCTTGAAAGTTGCCCAACGCAGACAATTGAGCTCTTTTTGAATGGAAATATTCCAGCTGATTTGGTAAACTCTTATTTGAAGCAGCATGCTCCAAACATGCAGGCCAGATACTTGGAACTTATGCTTGCTATGGATGAAAATGGGATATCCGGGAATCTGCAAAATGAAATGGTAAATATCTATCTATCAGAAGTCCTTGACTGGTATGCCGATTTAAGTGCTCAGCAGAAATGGGAGGAGCAAACTTACTCCATGACGAGAAAGAAATTATTGTCTGCTTTAGAGAGTATATCAGGTTATAGTCCAGAGGCTTTGCTGAAACGTCTTCCGTCTGATGCTTTATATGAAGAGCGTGCAATTTTGCTGGGCAAAATGAACCAACATGAGCTTGCCTTATCCCTATATGTTCACAAGCTTCATCTTCCTGAGATGGCACTGTCCTATTGTGATCGGGTGTATGATTCGTTGGCGCATCAACCGTCTTCAAGATCTTCTGGCAATATATACCTTACTCTATTGCAAATTTATCTTAATCCGAAGAGAACAACCAAGAACTTTGAGAAGCGGATTACAAATTTGGTATCACCGCAGAACATAGGAACACCAAAGGTTGGTTCTGCAAATTCAGTTAAAAGTAAAGGAGGTCGCGGAGCTAAGAAAATTGCTGCAATAGAGGTGGCAGATGACATAAGAGTCGGTCAAAGTGGCACTGACAGTAGCAGGAGTGATGGAGATGCAGATGCAGATGCAGATGCAGACGAGTCTGGTGAGGAAGGAGGGCCAGCAATCATGCTTGATGAGGTTCTTGATTTGTTGAGCAGAAGGTGGGACAGAATAAATGGAGCTCAAGCTCTCAAACTTTTGCCAAGGGAGACTAAACTACAGAACATGCTTCTGTTTATGGGACCTCTTTTGAGAAAATCCAGTGAAGCTTACAGGAACCTTTCAGTGATAAAAAGTTTAAGACAAAGCGATAACCTGCAGGGACTCCCAAAGTATGAAAGCAGTGGGCAGAGGATCGCCCTTAAGGAAGCGATGAAGACCAAGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
DUF223 Helitron_like_N PIF1 REPA_OB_2 Rep_fac-A_C

Protein Analysis

967

Amino Acids

107.08

Weight (kDa)

6.12

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNH PF00780 28 - 281 2.2e-20 CNH domain
Clathrin PF00637 505 - 599 8.3e-07 Region in Clathrin and VPS
Vps39_1 PF10366 508 - 618 1.3e-25 Vacuolar sorting protein 39 domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000050)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_2g06412 FvH4_2g08146 FvH4_2g10321 FvH4_2g13511 FvH4_2g20780 FvH4_2g20781 FvH4_2g22171 FvH4_2g25221 FvH4_3g03500 FvH4_3g07130 FvH4_3g10140 FvH4_3g10141 FvH4_3g24350 FvH4_4g08242 FvH4_4g13410 FvH4_4g18140 FvH4_4g22470 FvH4_5g19920 FvH4_5g21501 FvH4_5g26612 FvH4_5g29621 FvH4_5g31990 FvH4_6g00280 FvH4_6g00280 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g08820 FvH4_6g29600 FvH4_6g30401 FvH4_6g33630 FvH4_7g08310 FvH4_7g08311 FvH4_7g10871 FvH4_7g18890 FvH4_7g18891
malus_domestica MD01G1202900.v1.1 MD02G1249900.v1.1 MD03G1022200.v1.1 MD03G1164900.v1.1 MD04G1002000.v1.1 MD05G1167800.v1.1 MD05G1167900.v1.1 MD05G1202200.v1.1 MD05G1215800.v1.1 MD05G1328800.v1.1 MD07G1020000.v1.1 MD07G1020100.v1.1 MD07G1084700.v1.1 MD08G1007900.v1.1 MD08G1229500.v1.1 MD09G1016000.v1.1 MD09G1184200.v1.1 MD10G1198800.v1.1 MD10G1198900.v1.1 MD11G1203900.v1.1 MD12G1081100.v1.1 MD12G1101700.v1.1 MD13G1126700.v1.1 MD13G1158300.v1.1 MD13G1161500.v1.1 MD14G1083800.v1.1 MD14G1125700.v1.1 MD14G1159700.v1.1 MD14G1159800.v1.1 MD17G1252600.v1.1 MD17G1261900.v1.1 MD17G1262000.v1.1
pyrus_communis pycom01g12270 pycom02g14690 pycom02g17560 pycom02g26880 pycom02g26890 pycom03g13090 pycom03g19990 pycom03g20000 pycom04g00130 pycom04g05050 pycom04g10460 pycom05g27190 pycom07g05970 pycom08g02820 pycom08g11570 pycom09g07420 pycom09g07430 pycom09g08580 pycom10g06760 pycom10g11210 pycom10g11220 pycom11g14380 pycom11g21990 pycom12419g00230 pycom12494g00020 pycom14g06150 pycom15g09750 pycom15g20130 pycom15g27020 pycom15g27230 pycom16g17550 pycom16g18200 pycom5153684g00260 pycom520g00040
rosa_chinensis RchiOBHm_Chr1g0320331 RchiOBHm_Chr1g0329871 RchiOBHm_Chr1g0339991 RchiOBHm_Chr1g0371171 RchiOBHm_Chr1g0378741 RchiOBHm_Chr1g0378751 RchiOBHm_Chr1g0379271 RchiOBHm_Chr2g0122381 RchiOBHm_Chr2g0133251 RchiOBHm_Chr2g0133271 RchiOBHm_Chr2g0133331 RchiOBHm_Chr2g0135141 RchiOBHm_Chr2g0141711 RchiOBHm_Chr2g0145531 RchiOBHm_Chr2g0149141 RchiOBHm_Chr2g0149151 RchiOBHm_Chr2g0150581 RchiOBHm_Chr2g0159361 RchiOBHm_Chr2g0162161 RchiOBHm_Chr2g0174211 RchiOBHm_Chr3g0484061 RchiOBHm_Chr3g0491361 RchiOBHm_Chr4g0413141 RchiOBHm_Chr4g0444601 RchiOBHm_Chr5g0004901 RchiOBHm_Chr5g0004911 RchiOBHm_Chr5g0010461 RchiOBHm_Chr5g0027941 RchiOBHm_Chr5g0028311 RchiOBHm_Chr5g0030141 RchiOBHm_Chr5g0035621 RchiOBHm_Chr5g0050871 RchiOBHm_Chr5g0053321 RchiOBHm_Chr5g0060091 RchiOBHm_Chr5g0063301 RchiOBHm_Chr5g0066571 RchiOBHm_Chr5g0067561 RchiOBHm_Chr6g0262741 RchiOBHm_Chr7g0184001 RchiOBHm_Chr7g0206181 RchiOBHm_Chr7g0212031 RchiOBHm_Chr7g0214521 RchiOBHm_Chr7g0230521 RchiOBHm_Chr7g0230621
rosa_laevigata RLG00000014424 RLG00000022069 RLG00000036206
rosa_multiflora Rmu_sc0000121.1_g000006 Rmu_sc0000271.1_g000001 Rmu_sc0000293.1_g000010 Rmu_sc0000309.1_g000052 Rmu_sc0000556.1_g000045 Rmu_sc0000837.1_g000079 Rmu_sc0001069.1_g000026 Rmu_sc0001198.1_g000001 Rmu_sc0001353.1_g000031 Rmu_sc0001461.1_g000107 Rmu_sc0002011.1_g000038 Rmu_sc0003270.1_g000034 Rmu_sc0003874.1_g000017 Rmu_sc0004729.1_g000006 Rmu_sc0007116.1_g000001 Rmu_sc0009087.1_g000019 Rmu_sc0009877.1_g000004 Rmu_sc0015060.1_g000008 Rmu_sc0016097.1_g000001
rosa_roxburghii Rroxscaffold_1G00024140 Rroxscaffold_1G00027240 Rroxscaffold_1G00030710 Rroxscaffold_1G00035010 Rroxscaffold_1G00050490 Rroxscaffold_1G00069340 Rroxscaffold_1G00072870 Rroxscaffold_2G00078360 Rroxscaffold_2G00098490 Rroxscaffold_2G00106110 Rroxscaffold_2G00109450 Rroxscaffold_2G00125260 Rroxscaffold_2G00154260 Rroxscaffold_3G00221290 Rroxscaffold_3G00221300 Rroxscaffold_3G00232770 Rroxscaffold_3G00249670 Rroxscaffold_3G00255640 Rroxscaffold_3G00255820 Rroxscaffold_3G00255870 Rroxscaffold_3G00258160 Rroxscaffold_3G00258170 Rroxscaffold_3G00265290 Rroxscaffold_4G00320550 Rroxscaffold_4G00322930 Rroxscaffold_4G00331930 Rroxscaffold_4G00332460 Rroxscaffold_5G00337730 Rroxscaffold_5G00364590 Rroxscaffold_5G00365660 Rroxscaffold_5G00373900 Rroxscaffold_6G00395750 Rroxscaffold_6G00397090 Rroxscaffold_6G00407660 Rroxscaffold_6G00421590 Rroxscaffold_7G00156820 Rroxscaffold_7G00174040 Rroxscaffold_7G00175610 Rroxscaffold_7G00182780 Rroxscaffold_7G00210390 Rroxscaffold_7G00210490 Rroxscaffold_7G00214770 Rroxscaffold_7G00215580 Rroxscaffold_7G00215690
rosa_rugosa Rorug01G0099600 Rorug01G0127300 Rorug02G0145300 Rorug02G0145400 Rorug02G0145500.1 Rorug02G0145600 Rorug02G0145700 Rorug02G0145700 Rorug02G0278400 Rorug02G0444700 Rorug02G0542800 Rorug02G0547200 Rorug04G0015900 Rorug04G0082400 Rorug04G0297900 Rorug05G0078200 Rorug05G0207900 Rorug05G0277000 Rorug05G0528600 Rorug05G0528700 Rorug05G0528800 Rorug06G0001000 Rorug06G0246500 Rorug06G0246600 Rorug06G0246700 Rorug07G0189200 Rorug07G0189300 Rorug07G0189400 Rorug07G0189500 Rorug07G0212200 Rorug07G0212200 Rorug07G0221500 Rorug07G0248800
rosa_samantha Rh1AG011600 Rh1AG052100 Rh1AG163900 Rh1BG006600 Rh1BG025100 Rh1BG025200 Rh1BG032500 Rh1BG374400 Rh1BG374500 Rh1CG153000 Rh1DG148800 Rh1DG160400 Rh1DG181900 Rh1DG405100 Rh1DG445600 Rh2AG005600 Rh2AG137100 Rh2AG150500 Rh2AG150600 Rh2AG243200 Rh2AG264300 Rh2AG349900 Rh2AG387700 Rh2AG485100 Rh2CG115600 Rh2CG269600 Rh2CG294000 Rh2CG294100 Rh2CG317700 Rh2CG375000 Rh2CG471200 Rh2CG611400 Rh2DG070000 Rh2DG073900 Rh2DG234600 Rh3AG258200 Rh3CG251500 Rh3CG283900 Rh3CG331200 Rh3CG371500 Rh3DG077300 Rh3DG185900 Rh3DG332700 Rh3DG360400 Rh4AG105100 Rh4BG191100 Rh4DG139800 Rh4DG186000 Rh4DG187900 Rh5AG302000 Rh5AG431600 Rh5CG090000 Rh5CG214700 Rh5CG299100 Rh5CG561200 Rh5DG205600 Rh5DG244500 Rh5DG257300 Rh5DG257400 Rh5DG325900 Rh5DG335500 Rh5DG359300 Rh5DG364600 Rh5DG388600 Rh6AG013900 Rh6BG045400 Rh6CG031300 Rh6CG069100 Rh6CG157500 Rh6DG029800 Rh6DG066100 Rh6DG260800 Rh6DG320100 Rh7AG007600 Rh7BG046100 Rh7BG053900 Rh7BG204900 Rh7BG322600 Rh7BG421200 Rh7DG063200 Rh7DG233200 Rh7DG287000 Rh7DG289600 Rh7DG323500 Rh7DG323800 Rh7DG323900
rosa_wichuraiana Rw0G001160 Rw0G003580 Rw0G003590 Rw0G005910 Rw0G006230 Rw0G010240 Rw0G011660 Rw0G015630 Rw0G021440 Rw0G023690 Rw1G000060 Rw1G006170 Rw1G007940 Rw1G008630 Rw1G009460 Rw1G013800 Rw1G015100 Rw1G015710 Rw1G016040 Rw1G024780 Rw2G004590 Rw2G026770 Rw2G030550 Rw2G036430 Rw3G011440 Rw3G020600 Rw3G020710 Rw3G025390 Rw3G026320 Rw4G003380 Rw4G016140 Rw4G018350 Rw4G027460 Rw5G004690 Rw5G011140 Rw5G011820 Rw5G016560 Rw5G023230 Rw5G027290 Rw5G028520 Rw5G040540 Rw5G046050 Rw6G013650 Rw6G018020 Rw6G019800 Rw6G023450 Rw7G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2543
Acc36I ACCTGC 1 cut(s) 2838
AccB1I GGYRCC 4 cut(s) 320, 350, 715, 914
AccB7I CCANNNNNTGG 1 cut(s) 2462
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 2 cut(s) 381, 2499
AccIII TCCGGA 1 cut(s) 472
AclWI GGATC 6 cut(s) 102, 115, 1163, 1753, 1811, 2876
AcsI RAATTY 6 cut(s) 313, 461, 1427, 2373, 2425, 2473
AcuI CTGAAG 2 cut(s) 1008, 1551
AcyI GRCGYC 2 cut(s) 351, 716
AfaI GTAC 3 cut(s) 362, 927, 1119
AfiI CCNNNNNNNGG 7 cut(s) 184, 316, 317, 1503, 1504, 1808, 2462
AflII CTTAAG 1 cut(s) 2876
AflIII ACRYGT 1 cut(s) 1264
AjnI CCWGG 2 cut(s) 365, 398
AjuI GAANNNNNNNTTGG 2 cut(s) 2449, 2481
AleI CACNNNNGTG 1 cut(s) 432
Alw21I GWGCWC 5 cut(s) 33, 270, 1884, 2074, 2698
Alw26I GTCTC 3 cut(s) 899, 1416, 2717
AlwI GGATC 6 cut(s) 102, 115, 1163, 1753, 1811, 2876
Ama87I CYCGRG 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 6 cut(s) 76, 304, 342, 741, 1953, 2630
ApoI RAATTY 6 cut(s) 313, 461, 1427, 2373, 2425, 2473
ArsI GACNNNNNNTTYG 2 cut(s) 820, 852
Asp700I GAANNNNTTC 1 cut(s) 461
AspLEI GCGC 5 cut(s) 353, 718, 796, 1040, 2323
AspS9I GGNCC 5 cut(s) 77, 667, 783, 2630, 2756
AsuC2I CCSGG 3 cut(s) 217, 625, 2002
AsuHPI GGTGA 9 cut(s) 205, 328, 466, 493, 956, 1034, 1768, 2427, 2630
AsuII TTCGAA 1 cut(s) 826
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 3 cut(s) 667, 783, 2756
AxyI CCTNAGG 1 cut(s) 1752
BaeGI GKGCMC 2 cut(s) 323, 919
BamHI GGATCC 1 cut(s) 107
BanI GGYRCC 4 cut(s) 320, 350, 715, 914
BanII GRGCYC 4 cut(s) 33, 270, 1884, 2698
BbsI GAAGAC 6 cut(s) 833, 1466, 1557, 2168, 2325, 2897
Bbv12I GWGCWC 5 cut(s) 33, 270, 1884, 2074, 2698
BbvCI CCTCAGC 1 cut(s) 1386
BccI CCATC 8 cut(s) 71, 98, 332, 1053, 1177, 1504, 2275, 2573
BceAI ACGGC 4 cut(s) 91, 314, 319, 586
BcgI CGANNNNNNTGC 2 cut(s) 2051, 2085
BciT130I CCWGG 2 cut(s) 367, 400
BciVI GTATCC 2 cut(s) 1140, 1519
BclI TGATCA 1 cut(s) 333
BcnI CCSGG 3 cut(s) 217, 625, 2002
BcoDI GTCTC 3 cut(s) 899, 1416, 2717
BfaI CTAG 1 cut(s) 1503
BfmI CTRYAG 2 cut(s) 2732, 2831
BfoI RGCGCY 3 cut(s) 354, 719, 797
BfrI CTTAAG 1 cut(s) 2876
BfuAI ACCTGC 1 cut(s) 2838
BfuI GTATCC 2 cut(s) 1140, 1519
BglII AGATCT 1 cut(s) 2339
BlpI GCTNAGC 1 cut(s) 2073
Bme1390I CCNGG 5 cut(s) 217, 367, 400, 625, 2002
Bme18I GGWCC 3 cut(s) 667, 783, 2756
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 5 cut(s) 77, 667, 783, 2630, 2756
BmrFI CCNGG 5 cut(s) 217, 367, 400, 625, 2002
BmrI ACTGGG 1 cut(s) 1068
BmuI ACTGGG 1 cut(s) 1068
BpiI GAAGAC 6 cut(s) 833, 1466, 1557, 2168, 2325, 2897
BplI GAGNNNNNCTC 4 cut(s) 20, 52, 580, 612
BpmI CTGGAG 1 cut(s) 111
Bpu10I CCTNAGC 2 cut(s) 81, 1386
Bpu1102I GCTNAGC 1 cut(s) 2073
Bpu14I TTCGAA 1 cut(s) 826
BpuEI CTTGAG 4 cut(s) 1083, 1198, 1781, 2682
BpuMI CCSGG 3 cut(s) 217, 625, 2002
BsaHI GRCGYC 2 cut(s) 351, 716
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 8 cut(s) 91, 339, 345, 366, 379, 624, 1077, 2717
BsaWI WCCGGW 1 cut(s) 472
Bsc4I CCNNNNNNNGG 7 cut(s) 184, 316, 317, 1503, 1504, 1808, 2462
Bse1I ACTGG 7 cut(s) 562, 1063, 1487, 1527, 1561, 2057, 2775
Bse21I CCTNAGG 1 cut(s) 1752
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 2 cut(s) 367, 400
BseDI CCNNGG 8 cut(s) 91, 339, 345, 366, 379, 624, 1077, 2717
BseGI GGATG 5 cut(s) 481, 1078, 1417, 1433, 1990
BseLI CCNNNNNNNGG 7 cut(s) 184, 316, 317, 1503, 1504, 1808, 2462
BseMII CTCAG 4 cut(s) 1377, 1743, 2087, 2268
BseNI ACTGG 7 cut(s) 562, 1063, 1487, 1527, 1561, 2057, 2775
BseRI GAGGAG 4 cut(s) 80, 1295, 1637, 2102
BseSI GKGCMC 2 cut(s) 323, 919
BseYI CCCAGC 1 cut(s) 2215
BsgI GTGCAG 1 cut(s) 1239
Bsh1236I CGCG 2 cut(s) 381, 2499
Bsh1285I CGRYCG 1 cut(s) 376
BshFI GGCC 6 cut(s) 78, 306, 344, 743, 1955, 2632
BshNI GGYRCC 4 cut(s) 320, 350, 715, 914
BsiEI CGRYCG 1 cut(s) 376
BsiHKAI GWGCWC 5 cut(s) 33, 270, 1884, 2074, 2698
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 6 cut(s) 138, 216, 473, 624, 1775, 2001
BslI CCNNNNNNNGG 7 cut(s) 184, 316, 317, 1503, 1504, 1808, 2462
BsmAI GTCTC 3 cut(s) 899, 1416, 2717
BsmI GAATGC 2 cut(s) 1291, 1482
BsnI GGCC 6 cut(s) 78, 306, 344, 743, 1955, 2632
Bso31I GGTCTC 1 cut(s) 899
BsoBI CYCGRG 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 826
Bsp1286I GDGCHC 7 cut(s) 33, 270, 323, 919, 1884, 2074, 2698
Bsp13I TCCGGA 1 cut(s) 472
Bsp1407I TGTACA 1 cut(s) 925
Bsp143I GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
Bsp1720I GCTNAGC 1 cut(s) 2073
Bsp19I CCATGG 1 cut(s) 1077
BspANI GGCC 6 cut(s) 78, 306, 344, 743, 1955, 2632
BspCNI CTCAG 4 cut(s) 1378, 1744, 2086, 2269
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 2 cut(s) 381, 2499
BspHI TCATGA 1 cut(s) 688
BspMAI CTGCAG 1 cut(s) 2835
BspMI ACCTGC 1 cut(s) 2838
BspPI GGATC 6 cut(s) 102, 115, 1163, 1753, 1811, 2876
BspQI GCTCTTC 1 cut(s) 2193
BspT104I TTCGAA 1 cut(s) 826
BspT107I GGYRCC 4 cut(s) 320, 350, 715, 914
BspTI CTTAAG 1 cut(s) 2876
BspTNI GGTCTC 1 cut(s) 899
BsrBI CCGCTC 1 cut(s) 147
BsrGI TGTACA 1 cut(s) 925
BsrI ACTGG 7 cut(s) 562, 1063, 1487, 1527, 1561, 2057, 2775
BssECI CCNNGG 8 cut(s) 91, 339, 345, 366, 379, 624, 1077, 2717
BssMI GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
BssNI GRCGYC 2 cut(s) 351, 716
BssT1I CCWWGG 4 cut(s) 339, 345, 1077, 2717
Bst2UI CCWGG 2 cut(s) 367, 400
Bst6I CTCTTC 7 cut(s) 433, 621, 902, 1392, 1719, 2193, 2384
BstACI GRCGYC 2 cut(s) 351, 716
BstAFI CTTAAG 1 cut(s) 2876
BstAUI TGTACA 1 cut(s) 925
BstBI TTCGAA 1 cut(s) 826
BstC8I GCNNGC 8 cut(s) 594, 997, 1938, 1953, 1977, 2205, 2241, 2634
BstDEI CTNAG 9 cut(s) 81, 225, 1001, 1149, 1386, 1752, 2073, 2277, 2505
BstDSI CCRYGG 2 cut(s) 379, 1077
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 5 cut(s) 481, 1078, 1417, 1433, 1990
BstFNI CGCG 2 cut(s) 381, 2499
BstH2I RGCGCY 3 cut(s) 354, 719, 797
BstHHI GCGC 5 cut(s) 353, 718, 796, 1040, 2323
BstKTI GATC 9 cut(s) 57, 110, 336, 1171, 1761, 1819, 2302, 2342, 2871
BstMAI GTCTC 3 cut(s) 899, 1416, 2717
BstMBI GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
BstMCI CGRYCG 1 cut(s) 376
BstMWI GCNNNNNNNGC 5 cut(s) 350, 598, 869, 982, 1016
BstNI CCWGG 2 cut(s) 367, 400
BstNSI RCATGY 4 cut(s) 1268, 1940, 1951, 2743
BstSCI CCNGG 5 cut(s) 215, 365, 398, 623, 2000
BstSFI CTRYAG 2 cut(s) 2732, 2831
BstSLI GKGCMC 2 cut(s) 323, 919
BstUI CGCG 2 cut(s) 381, 2499
BstV2I GAAGAC 6 cut(s) 833, 1466, 1557, 2168, 2325, 2897
BstX2I RGATCY 2 cut(s) 107, 2339
BstXI CCANNNNNNTGG 1 cut(s) 1963
BstYI RGATCY 2 cut(s) 107, 2339
Bsu36I CCTNAGG 1 cut(s) 1752
BsuI GTATCC 2 cut(s) 1140, 1519
BsuRI GGCC 6 cut(s) 78, 306, 344, 743, 1955, 2632
BtgI CCRYGG 2 cut(s) 379, 1077
BtgZI GCGATG 2 cut(s) 310, 2900
BtsCI GGATG 5 cut(s) 481, 1078, 1417, 1433, 1990
BtsI GCAGTG 1 cut(s) 2863
BtsIMutI CAGTG 8 cut(s) 16, 1256, 1812, 2285, 2559, 2782, 2805, 2863
BveI ACCTGC 1 cut(s) 2838
Cac8I GCNNGC 8 cut(s) 594, 997, 1938, 1953, 1977, 2205, 2241, 2634
CciI TCATGA 1 cut(s) 688
CfoI GCGC 5 cut(s) 353, 718, 796, 1040, 2323
Cfr13I GGNCC 5 cut(s) 77, 667, 783, 2630, 2756
Cfr42I CCGCGG 1 cut(s) 382
Csp6I GTAC 3 cut(s) 361, 926, 1118
CspCI CAANNNNNGTGG 4 cut(s) 1456, 1491, 2508, 2543
CviQI GTAC 3 cut(s) 361, 926, 1118
DdeI CTNAG 9 cut(s) 81, 225, 1001, 1149, 1386, 1752, 2073, 2277, 2505
DinI GGCGCC 2 cut(s) 352, 717
DpnI GATC 9 cut(s) 56, 109, 335, 1170, 1760, 1818, 2301, 2341, 2870
DpnII GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
DrdI GACNNNNNNGTC 1 cut(s) 2543
DseDI GACNNNNNNGTC 1 cut(s) 2543
Eam1104I CTCTTC 7 cut(s) 433, 621, 902, 1392, 1719, 2193, 2384
EarI CTCTTC 7 cut(s) 433, 621, 902, 1392, 1719, 2193, 2384
EciI GGCGGA 2 cut(s) 588, 972
Ecl136II GAGCTC 4 cut(s) 31, 268, 1882, 2696
Eco130I CCWWGG 4 cut(s) 339, 345, 1077, 2717
Eco147I AGGCCT 1 cut(s) 743
Eco24I GRGCYC 4 cut(s) 33, 270, 1884, 2698
Eco31I GGTCTC 1 cut(s) 899
Eco32I GATATC 3 cut(s) 294, 1164, 1998
Eco47I GGWCC 3 cut(s) 667, 783, 2756
Eco53kI GAGCTC 4 cut(s) 31, 268, 1882, 2696
Eco57I CTGAAG 2 cut(s) 1008, 1551
Eco81I CCTNAGG 1 cut(s) 1752
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 4 cut(s) 31, 268, 1882, 2696
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 2756
EcoRI GAATTC 1 cut(s) 461
EcoRII CCWGG 2 cut(s) 365, 398
EcoRV GATATC 3 cut(s) 294, 1164, 1998
EcoT14I CCWWGG 4 cut(s) 339, 345, 1077, 2717
EcoT38I GRGCYC 4 cut(s) 33, 270, 1884, 2698
EgeI GGCGCC 2 cut(s) 352, 717
EheI GGCGCC 2 cut(s) 352, 717
ErhI CCWWGG 4 cut(s) 339, 345, 1077, 2717
FalI AAGNNNNNCTT 4 cut(s) 984, 1016, 1533, 1565
FauI CCCGC 1 cut(s) 152
FbaI TGATCA 1 cut(s) 333
FokI GGATG 5 cut(s) 488, 1085, 1420, 1424, 1997
FriOI GRGCYC 4 cut(s) 33, 270, 1884, 2698
FspBI CTAG 1 cut(s) 1503
GlaI GCGC 5 cut(s) 352, 717, 795, 1039, 2322
GsaI CCCAGC 1 cut(s) 2219
GsuI CTGGAG 1 cut(s) 111
HaeII RGCGCY 3 cut(s) 354, 719, 797
HaeIII GGCC 6 cut(s) 78, 306, 344, 743, 1955, 2632
HapII CCGG 6 cut(s) 138, 216, 473, 624, 1775, 2001
HhaI GCGC 5 cut(s) 353, 718, 796, 1040, 2323
Hin1I GRCGYC 2 cut(s) 351, 716
Hin6I GCGC 5 cut(s) 351, 716, 794, 1038, 2321
HinP1I GCGC 5 cut(s) 351, 716, 794, 1038, 2321
HindIII AAGCTT 4 cut(s) 83, 957, 2264, 2781
HpaII CCGG 6 cut(s) 138, 216, 473, 624, 1775, 2001
HphI GGTGA 9 cut(s) 205, 328, 466, 493, 956, 1034, 1768, 2427, 2630
Hpy166II GTNNAC 5 cut(s) 7, 361, 667, 1918, 2260
Hpy8I GTNNAC 5 cut(s) 7, 361, 667, 1918, 2260
Hpy99I CGWCG 3 cut(s) 29, 434, 840
HpyAV CCTTC 9 cut(s) 451, 591, 630, 637, 686, 733, 1143, 2618, 2667
HpyCH4IV ACGT 1 cut(s) 2173
HpyF10VI GCNNNNNNNGC 5 cut(s) 350, 598, 869, 982, 1016
HpyF3I CTNAG 9 cut(s) 81, 225, 1001, 1149, 1386, 1752, 2073, 2277, 2505
HpySE526I ACGT 1 cut(s) 2173
Hsp92I GRCGYC 2 cut(s) 351, 716
HspAI GCGC 5 cut(s) 351, 716, 794, 1038, 2321
KasI GGCGCC 2 cut(s) 350, 715
Kpn2I TCCGGA 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 333
KspI CCGCGG 1 cut(s) 382
Kzo9I GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
LguI GCTCTTC 1 cut(s) 2193
MaeI CTAG 1 cut(s) 1503
MaeII ACGT 1 cut(s) 2173
MaeIII GTNAC 2 cut(s) 1260, 1489
MalI GATC 9 cut(s) 56, 109, 335, 1170, 1760, 1818, 2301, 2341, 2870
MbiI CCGCTC 1 cut(s) 147
MboI GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
MfeI CAATTG 2 cut(s) 1782, 1875
MflI RGATCY 2 cut(s) 107, 2339
MhlI GDGCHC 7 cut(s) 33, 270, 323, 919, 1884, 2074, 2698
Mly113I GGCGCC 2 cut(s) 351, 716
MlyI GAGTC 7 cut(s) 1307, 1451, 1736, 1787, 2553, 2621, 2832
MmeI TCCRAC 4 cut(s) 63, 183, 448, 1399
MroI TCCGGA 1 cut(s) 472
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 8 cut(s) 932, 1338, 2066, 2382, 2481, 2813, 2877, 2902
MslI CAYNNNNRTG 3 cut(s) 329, 432, 1325
MspA1I CMGCKG 4 cut(s) 235, 381, 1571, 1907
MspCI CTTAAG 1 cut(s) 2876
MspI CCGG 6 cut(s) 138, 216, 473, 624, 1775, 2001
MspR9I CCNGG 5 cut(s) 217, 367, 400, 625, 2002
MunI CAATTG 2 cut(s) 1782, 1875
Mva1269I GAATGC 2 cut(s) 1291, 1482
MvaI CCWGG 2 cut(s) 367, 400
MvnI CGCG 2 cut(s) 381, 2499
MwoI GCNNNNNNNGC 5 cut(s) 350, 598, 869, 982, 1016
NarI GGCGCC 2 cut(s) 351, 716
NciI CCSGG 3 cut(s) 217, 625, 2002
NcoI CCATGG 1 cut(s) 1077
NdeII GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 1 cut(s) 1260
NspI RCATGY 4 cut(s) 1268, 1940, 1951, 2743
NspV TTCGAA 1 cut(s) 826
OliI CACNNNNGTG 1 cut(s) 432
PaeI GCATGC 1 cut(s) 1940
PaeR7I CTCGAG 1 cut(s) 200
PagI TCATGA 1 cut(s) 688
PceI AGGCCT 1 cut(s) 743
PciI ACATGT 1 cut(s) 1264
PciSI GCTCTTC 1 cut(s) 2193
PcsI WCGNNNNNNNCGW 5 cut(s) 24, 63, 99, 787, 832
PctI GAATGC 2 cut(s) 1291, 1482
PdmI GAANNNNTTC 1 cut(s) 461
PflMI CCANNNNNTGG 1 cut(s) 2462
PfoI TCCNGGA 1 cut(s) 2000
PleI GAGTC 7 cut(s) 1306, 1450, 1735, 1786, 2552, 2620, 2832
PluTI GGCGCC 2 cut(s) 354, 719
PpsI GAGTC 7 cut(s) 1306, 1450, 1735, 1786, 2552, 2620, 2832
PpuMI RGGWCCY 1 cut(s) 2756
PscI ACATGT 1 cut(s) 1264
Psp124BI GAGCTC 4 cut(s) 33, 270, 1884, 2698
Psp5II RGGWCCY 1 cut(s) 2756
Psp6I CCWGG 2 cut(s) 365, 398
PspFI CCCAGC 1 cut(s) 2215
PspGI CCWGG 2 cut(s) 365, 398
PspPI GGNCC 5 cut(s) 77, 667, 783, 2630, 2756
PspPPI RGGWCCY 1 cut(s) 2756
PstI CTGCAG 1 cut(s) 2835
PsuI RGATCY 2 cut(s) 107, 2339
PvuII CAGCTG 1 cut(s) 1907
RsaI GTAC 3 cut(s) 362, 927, 1119
RsaNI GTAC 3 cut(s) 361, 926, 1118
RseI CAYNNNNRTG 3 cut(s) 329, 432, 1325
SacI GAGCTC 4 cut(s) 33, 270, 1884, 2698
SacII CCGCGG 1 cut(s) 382
SapI GCTCTTC 1 cut(s) 2193
SaqAI TTAA 8 cut(s) 932, 1338, 2066, 2382, 2481, 2813, 2877, 2902
Sau3AI GATC 9 cut(s) 54, 107, 333, 1168, 1758, 1816, 2299, 2339, 2868
Sau96I GGNCC 5 cut(s) 77, 667, 783, 2630, 2756
SbfI CCTGCAGG 1 cut(s) 2835
SchI GAGTC 7 cut(s) 1307, 1451, 1736, 1787, 2553, 2621, 2832
ScrFI CCNGG 5 cut(s) 217, 367, 400, 625, 2002
SdaI CCTGCAGG 1 cut(s) 2835
SduI GDGCHC 7 cut(s) 33, 270, 323, 919, 1884, 2074, 2698
SfcI CTRYAG 2 cut(s) 2732, 2831
SfoI GGCGCC 2 cut(s) 352, 717
Sfr274I CTCGAG 1 cut(s) 200
Sfr303I CCGCGG 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 826
SgrBI CCGCGG 1 cut(s) 382
SinI GGWCC 3 cut(s) 667, 783, 2756
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 3 cut(s) 329, 432, 1325
SmlI CTYRAG 6 cut(s) 200, 1098, 1213, 1796, 2697, 2876
SmoI CTYRAG 6 cut(s) 200, 1098, 1213, 1796, 2697, 2876
SphI GCATGC 1 cut(s) 1940
Sse8387I CCTGCAGG 1 cut(s) 2835
SseBI AGGCCT 1 cut(s) 743
SspDI GGCGCC 2 cut(s) 350, 715
SspI AATATT 1 cut(s) 1900
SspMI CTAG 1 cut(s) 1503
SstI GAGCTC 4 cut(s) 33, 270, 1884, 2698
StuI AGGCCT 1 cut(s) 743
StyD4I CCNGG 5 cut(s) 215, 365, 398, 623, 2000
StyI CCWWGG 4 cut(s) 339, 345, 1077, 2717
TaiI ACGT 1 cut(s) 2176
TaqI TCGA 6 cut(s) 27, 57, 66, 201, 764, 826
TaqII GACCGA 2 cut(s) 771, 2537
TatI WGTACW 3 cut(s) 360, 925, 1117
TauI GCSGC 3 cut(s) 235, 381, 1574
Tru1I TTAA 8 cut(s) 932, 1338, 2066, 2382, 2481, 2813, 2877, 2902
Tru9I TTAA 8 cut(s) 932, 1338, 2066, 2382, 2481, 2813, 2877, 2902
TscAI CASTG 8 cut(s) 16, 1263, 1819, 2292, 2566, 2782, 2805, 2863
TseFI GTSAC 1 cut(s) 1260
Tsp45I GTSAC 1 cut(s) 1260
TspGWI ACGGA 2 cut(s) 13, 2169
TspRI CASTG 8 cut(s) 16, 1263, 1819, 2292, 2566, 2782, 2805, 2863
Van91I CCANNNNNTGG 1 cut(s) 2462
Vha464I CTTAAG 1 cut(s) 2876
VpaK11BI GGWCC 3 cut(s) 667, 783, 2756
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 6 cut(s) 313, 461, 1427, 2373, 2425, 2473
XceI RCATGY 4 cut(s) 1268, 1940, 1951, 2743
XcmI CCANNNNNNNNNTGG 1 cut(s) 1828
XhoI CTCGAG 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 1 cut(s) 1503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.