DNA recombination
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 3 cut(s) | 218, 746, 968 |
| AccIII | TCCGGA | 1 cut(s) | 1082 |
| AciI | CCGC | 2 cut(s) | 1492, 1526 |
| AclI | AACGTT | 1 cut(s) | 474 |
| AclWI | GGATC | 3 cut(s) | 244, 1335, 1348 |
| AcsI | RAATTY | 2 cut(s) | 19, 138 |
| AfaI | GTAC | 3 cut(s) | 280, 346, 864 |
| AgsI | TTSAA | 8 cut(s) | 178, 499, 832, 1389, 1511, 1582, 1600, 1649 |
| AjnI | CCWGG | 1 cut(s) | 1279 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 27, 59 |
| AluBI | AGCT | 6 cut(s) | 165, 326, 838, 1265, 1432, 1555 |
| AluI | AGCT | 6 cut(s) | 165, 326, 838, 1265, 1432, 1555 |
| Alw21I | GWGCWC | 2 cut(s) | 167, 1333 |
| Alw26I | GTCTC | 1 cut(s) | 512 |
| AlwI | GGATC | 3 cut(s) | 244, 1335, 1348 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 95, 326 |
| Aor13HI | TCCGGA | 1 cut(s) | 1082 |
| AoxI | GGCC | 1 cut(s) | 57 |
| ApeKI | GCWGC | 7 cut(s) | 858, 1191, 1265, 1268, 1367, 1432, 1489 |
| ApoI | RAATTY | 2 cut(s) | 19, 138 |
| AseI | ATTAAT | 5 cut(s) | 366, 699, 810, 921, 1032 |
| AspS9I | GGNCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| AsuC2I | CCSGG | 1 cut(s) | 150 |
| AsuHPI | GGTGA | 1 cut(s) | 500 |
| AvaII | GGWCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| BaeGI | GKGCMC | 2 cut(s) | 150, 1638 |
| BamHI | GGATCC | 1 cut(s) | 1340 |
| BanI | GGYRCC | 3 cut(s) | 218, 746, 968 |
| BanII | GRGCYC | 1 cut(s) | 167 |
| BbsI | GAAGAC | 1 cut(s) | 110 |
| Bbv12I | GWGCWC | 2 cut(s) | 167, 1333 |
| BbvCI | CCTCAGC | 1 cut(s) | 54 |
| BbvI | GCAGC | 7 cut(s) | 870, 1203, 1252, 1280, 1354, 1419, 1476 |
| BccI | CCATC | 5 cut(s) | 368, 479, 590, 1280, 1297 |
| BciT130I | CCWGG | 1 cut(s) | 1281 |
| BciVI | GTATCC | 1 cut(s) | 1102 |
| BcnI | CCSGG | 1 cut(s) | 150 |
| BcoDI | GTCTC | 1 cut(s) | 512 |
| BfaI | CTAG | 1 cut(s) | 207 |
| BfmI | CTRYAG | 2 cut(s) | 1266, 1433 |
| BfuI | GTATCC | 1 cut(s) | 1102 |
| BglII | AGATCT | 1 cut(s) | 1655 |
| BisI | GCNGC | 7 cut(s) | 859, 1192, 1266, 1269, 1368, 1433, 1490 |
| BlpI | GCTNAGC | 1 cut(s) | 839 |
| BlsI | GCNGC | 7 cut(s) | 860, 1193, 1267, 1270, 1369, 1434, 1491 |
| BmcAI | AGTACT | 2 cut(s) | 280, 346 |
| Bme1390I | CCNGG | 2 cut(s) | 150, 1281 |
| Bme18I | GGWCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| BmgT120I | GGNCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| BmrFI | CCNGG | 2 cut(s) | 150, 1281 |
| BmrI | ACTGGG | 1 cut(s) | 267 |
| BmsI | GCATC | 5 cut(s) | 304, 392, 580, 614, 1476 |
| BmuI | ACTGGG | 1 cut(s) | 267 |
| BpiI | GAAGAC | 1 cut(s) | 110 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 154, 186 |
| BpmI | CTGGAG | 1 cut(s) | 1583 |
| Bpu10I | CCTNAGC | 1 cut(s) | 54 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 839 |
| BpuEI | CTTGAG | 1 cut(s) | 1466 |
| BpuMI | CCSGG | 1 cut(s) | 150 |
| BsaJI | CCNNGG | 3 cut(s) | 1069, 1256, 1503 |
| BsaWI | WCCGGW | 5 cut(s) | 416, 638, 749, 971, 1082 |
| Bse1I | ACTGG | 3 cut(s) | 143, 273, 1566 |
| Bse3DI | GCAATG | 1 cut(s) | 850 |
| BseAI | TCCGGA | 1 cut(s) | 1082 |
| BseBI | CCWGG | 1 cut(s) | 1281 |
| BseDI | CCNNGG | 3 cut(s) | 1069, 1256, 1503 |
| BseGI | GGATG | 2 cut(s) | 1173, 1272 |
| BseMI | GCAATG | 1 cut(s) | 850 |
| BseMII | CTCAG | 3 cut(s) | 45, 228, 1650 |
| BseNI | ACTGG | 3 cut(s) | 143, 273, 1566 |
| BseRI | GAGGAG | 2 cut(s) | 1341, 1492 |
| BseSI | GKGCMC | 2 cut(s) | 150, 1638 |
| BseXI | GCAGC | 7 cut(s) | 870, 1203, 1252, 1280, 1354, 1419, 1476 |
| BseYI | CCCAGC | 1 cut(s) | 158 |
| BshFI | GGCC | 1 cut(s) | 59 |
| BshNI | GGYRCC | 3 cut(s) | 218, 746, 968 |
| BsiHKAI | GWGCWC | 2 cut(s) | 167, 1333 |
| BsiSI | CCGG | 6 cut(s) | 150, 417, 639, 750, 972, 1083 |
| BslFI | GGGAC | 1 cut(s) | 1407 |
| BsmAI | GTCTC | 1 cut(s) | 512 |
| BsmFI | GGGAC | 1 cut(s) | 1407 |
| BsnI | GGCC | 1 cut(s) | 59 |
| Bsp1286I | GDGCHC | 4 cut(s) | 150, 167, 1333, 1638 |
| Bsp13I | TCCGGA | 1 cut(s) | 1082 |
| Bsp143I | GATC | 3 cut(s) | 249, 1340, 1655 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 839 |
| BspACI | CCGC | 2 cut(s) | 1492, 1526 |
| BspANI | GGCC | 1 cut(s) | 59 |
| BspCNI | CTCAG | 3 cut(s) | 46, 229, 1651 |
| BspEI | TCCGGA | 1 cut(s) | 1082 |
| BspHI | TCATGA | 1 cut(s) | 73 |
| BspMAI | CTGCAG | 2 cut(s) | 1270, 1437 |
| BspPI | GGATC | 3 cut(s) | 244, 1335, 1348 |
| BspQI | GCTCTTC | 1 cut(s) | 172 |
| BspT107I | GGYRCC | 3 cut(s) | 218, 746, 968 |
| BsrDI | GCAATG | 1 cut(s) | 850 |
| BsrI | ACTGG | 3 cut(s) | 143, 273, 1566 |
| BssECI | CCNNGG | 3 cut(s) | 1069, 1256, 1503 |
| BssMI | GATC | 3 cut(s) | 249, 1340, 1655 |
| BssT1I | CCWWGG | 2 cut(s) | 1069, 1256 |
| Bst2UI | CCWGG | 1 cut(s) | 1281 |
| Bst4CI | ACNGT | 7 cut(s) | 109, 448, 559, 670, 677, 1225, 1310 |
| Bst6I | CTCTTC | 4 cut(s) | 172, 843, 1410, 1650 |
| BstDEI | CTNAG | 8 cut(s) | 54, 237, 395, 617, 839, 1212, 1514, 1659 |
| BstF5I | GGATG | 2 cut(s) | 1173, 1272 |
| BstKTI | GATC | 3 cut(s) | 252, 1343, 1658 |
| BstMAI | GTCTC | 1 cut(s) | 512 |
| BstMBI | GATC | 3 cut(s) | 249, 1340, 1655 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1367, 1438 |
| BstNI | CCWGG | 1 cut(s) | 1281 |
| BstNSI | RCATGY | 1 cut(s) | 1526 |
| BstSCI | CCNGG | 2 cut(s) | 148, 1279 |
| BstSFI | CTRYAG | 2 cut(s) | 1266, 1433 |
| BstSLI | GKGCMC | 2 cut(s) | 150, 1638 |
| BstV1I | GCAGC | 7 cut(s) | 870, 1203, 1252, 1280, 1354, 1419, 1476 |
| BstV2I | GAAGAC | 1 cut(s) | 110 |
| BstX2I | RGATCY | 2 cut(s) | 1340, 1655 |
| BstYI | RGATCY | 2 cut(s) | 1340, 1655 |
| BsuI | GTATCC | 1 cut(s) | 1102 |
| BsuRI | GGCC | 1 cut(s) | 59 |
| BtsCI | GGATG | 2 cut(s) | 1173, 1272 |
| BtsIMutI | CAGTG | 3 cut(s) | 150, 682, 1559 |
| CaiI | CAGNNNCTG | 2 cut(s) | 95, 326 |
| CciI | TCATGA | 1 cut(s) | 73 |
| Cfr13I | GGNCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| CpoI | CGGWCCG | 2 cut(s) | 419, 641 |
| CseI | GACGC | 1 cut(s) | 338 |
| Csp6I | GTAC | 3 cut(s) | 279, 345, 863 |
| CspI | CGGWCCG | 2 cut(s) | 419, 641 |
| CviAII | CATG | 3 cut(s) | 74, 1469, 1523 |
| CviQI | GTAC | 3 cut(s) | 279, 345, 863 |
| DdeI | CTNAG | 8 cut(s) | 54, 237, 395, 617, 839, 1212, 1514, 1659 |
| DpnI | GATC | 3 cut(s) | 251, 1342, 1657 |
| DpnII | GATC | 3 cut(s) | 249, 1340, 1655 |
| DraI | TTTAAA | 1 cut(s) | 1630 |
| Eam1104I | CTCTTC | 4 cut(s) | 172, 843, 1410, 1650 |
| EarI | CTCTTC | 4 cut(s) | 172, 843, 1410, 1650 |
| Ecl136II | GAGCTC | 1 cut(s) | 165 |
| Eco130I | CCWWGG | 2 cut(s) | 1069, 1256 |
| Eco24I | GRGCYC | 1 cut(s) | 167 |
| Eco32I | GATATC | 4 cut(s) | 426, 537, 648, 870 |
| Eco47I | GGWCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| Eco53kI | GAGCTC | 1 cut(s) | 165 |
| EcoICRI | GAGCTC | 1 cut(s) | 165 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1216 |
| EcoRI | GAATTC | 1 cut(s) | 138 |
| EcoRII | CCWGG | 1 cut(s) | 1279 |
| EcoRV | GATATC | 4 cut(s) | 426, 537, 648, 870 |
| EcoT14I | CCWWGG | 2 cut(s) | 1069, 1256 |
| EcoT38I | GRGCYC | 1 cut(s) | 167 |
| ErhI | CCWWGG | 2 cut(s) | 1069, 1256 |
| FaeI | CATG | 3 cut(s) | 77, 1472, 1526 |
| FaqI | GGGAC | 1 cut(s) | 1407 |
| FatI | CATG | 3 cut(s) | 73, 1468, 1522 |
| Fnu4HI | GCNGC | 7 cut(s) | 859, 1192, 1266, 1269, 1368, 1433, 1490 |
| FokI | GGATG | 2 cut(s) | 1180, 1259 |
| FriOI | GRGCYC | 1 cut(s) | 167 |
| Fsp4HI | GCNGC | 7 cut(s) | 859, 1192, 1266, 1269, 1368, 1433, 1490 |
| FspBI | CTAG | 1 cut(s) | 207 |
| GluI | GCNGC | 7 cut(s) | 859, 1192, 1266, 1269, 1368, 1433, 1490 |
| GsaI | CCCAGC | 1 cut(s) | 162 |
| GsuI | CTGGAG | 1 cut(s) | 1583 |
| HaeIII | GGCC | 1 cut(s) | 59 |
| HapII | CCGG | 6 cut(s) | 150, 417, 639, 750, 972, 1083 |
| HgaI | GACGC | 1 cut(s) | 338 |
| Hin1II | CATG | 3 cut(s) | 77, 1472, 1526 |
| HincII | GTYRAC | 1 cut(s) | 1063 |
| HindII | GTYRAC | 1 cut(s) | 1063 |
| HindIII | AAGCTT | 1 cut(s) | 836 |
| HpaI | GTTAAC | 1 cut(s) | 1063 |
| HpaII | CCGG | 6 cut(s) | 150, 417, 639, 750, 972, 1083 |
| HphI | GGTGA | 1 cut(s) | 500 |
| Hpy166II | GTNNAC | 3 cut(s) | 673, 900, 1063 |
| Hpy188I | TCNGA | 6 cut(s) | 238, 322, 338, 1318, 1465, 1660 |
| Hpy188III | TCNNGA | 5 cut(s) | 74, 257, 1083, 1483, 1649 |
| Hpy8I | GTNNAC | 3 cut(s) | 673, 900, 1063 |
| HpyAV | CCTTC | 2 cut(s) | 172, 1383 |
| HpyCH4III | ACNGT | 7 cut(s) | 109, 448, 559, 670, 677, 1225, 1310 |
| HpyCH4IV | ACGT | 1 cut(s) | 474 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1367, 1438 |
| HpyF3I | CTNAG | 8 cut(s) | 54, 237, 395, 617, 839, 1212, 1514, 1659 |
| HpySE526I | ACGT | 1 cut(s) | 474 |
| Hsp92II | CATG | 3 cut(s) | 77, 1472, 1526 |
| Kpn2I | TCCGGA | 1 cut(s) | 1082 |
| KspAI | GTTAAC | 1 cut(s) | 1063 |
| Kzo9I | GATC | 3 cut(s) | 249, 1340, 1655 |
| LguI | GCTCTTC | 1 cut(s) | 172 |
| Lsp1109I | GCAGC | 7 cut(s) | 870, 1203, 1252, 1280, 1354, 1419, 1476 |
| LweI | GCATC | 5 cut(s) | 304, 392, 580, 614, 1476 |
| MaeI | CTAG | 1 cut(s) | 207 |
| MaeII | ACGT | 1 cut(s) | 474 |
| MaeIII | GTNAC | 1 cut(s) | 1494 |
| MalI | GATC | 3 cut(s) | 251, 1342, 1657 |
| MboI | GATC | 3 cut(s) | 249, 1340, 1655 |
| MboII | GAAGA | 7 cut(s) | 115, 159, 511, 844, 860, 1427, 1637 |
| MflI | RGATCY | 2 cut(s) | 1340, 1655 |
| MhlI | GDGCHC | 4 cut(s) | 150, 167, 1333, 1638 |
| MluCI | AATT | 8 cut(s) | 19, 138, 199, 504, 726, 1029, 1451, 1548 |
| MmeI | TCCRAC | 1 cut(s) | 1515 |
| MroI | TCCGGA | 1 cut(s) | 1082 |
| MspA1I | CMGCKG | 1 cut(s) | 1432 |
| MspI | CCGG | 6 cut(s) | 150, 417, 639, 750, 972, 1083 |
| MspR9I | CCNGG | 2 cut(s) | 150, 1281 |
| MvaI | CCWGG | 1 cut(s) | 1281 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1367, 1438 |
| NciI | CCSGG | 1 cut(s) | 150 |
| NdeII | GATC | 3 cut(s) | 249, 1340, 1655 |
| NlaIII | CATG | 3 cut(s) | 77, 1472, 1526 |
| NmeAIII | GCCGAG | 1 cut(s) | 85 |
| NmuCI | GTSAC | 1 cut(s) | 1494 |
| NspI | RCATGY | 1 cut(s) | 1526 |
| PagI | TCATGA | 1 cut(s) | 73 |
| PciSI | GCTCTTC | 1 cut(s) | 172 |
| PflFI | GACNNNGTC | 1 cut(s) | 332 |
| PfoI | TCCNGGA | 1 cut(s) | 1279 |
| PkrI | GCNGC | 7 cut(s) | 860, 1193, 1267, 1270, 1369, 1434, 1491 |
| PpuMI | RGGWCCY | 1 cut(s) | 1216 |
| PshBI | ATTAAT | 5 cut(s) | 366, 699, 810, 921, 1032 |
| Psp124BI | GAGCTC | 1 cut(s) | 167 |
| Psp1406I | AACGTT | 1 cut(s) | 474 |
| Psp5II | RGGWCCY | 1 cut(s) | 1216 |
| Psp6I | CCWGG | 1 cut(s) | 1279 |
| PspFI | CCCAGC | 1 cut(s) | 158 |
| PspGI | CCWGG | 1 cut(s) | 1279 |
| PspPI | GGNCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| PspPPI | RGGWCCY | 1 cut(s) | 1216 |
| PstI | CTGCAG | 2 cut(s) | 1270, 1437 |
| PstNI | CAGNNNCTG | 2 cut(s) | 95, 326 |
| PsuI | RGATCY | 2 cut(s) | 1340, 1655 |
| PsyI | GACNNNGTC | 1 cut(s) | 332 |
| PvuII | CAGCTG | 1 cut(s) | 1432 |
| RsaI | GTAC | 3 cut(s) | 280, 346, 864 |
| RsaNI | GTAC | 3 cut(s) | 279, 345, 863 |
| Rsr2I | CGGWCCG | 2 cut(s) | 419, 641 |
| RsrII | CGGWCCG | 2 cut(s) | 419, 641 |
| SacI | GAGCTC | 1 cut(s) | 167 |
| SapI | GCTCTTC | 1 cut(s) | 172 |
| SatI | GCNGC | 7 cut(s) | 859, 1192, 1266, 1269, 1368, 1433, 1490 |
| Sau3AI | GATC | 3 cut(s) | 249, 1340, 1655 |
| Sau96I | GGNCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| ScaI | AGTACT | 2 cut(s) | 280, 346 |
| ScrFI | CCNGG | 2 cut(s) | 150, 1281 |
| SduI | GDGCHC | 4 cut(s) | 150, 167, 1333, 1638 |
| SfaNI | GCATC | 5 cut(s) | 304, 392, 580, 614, 1476 |
| SfcI | CTRYAG | 2 cut(s) | 1266, 1433 |
| SinI | GGWCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| SmlI | CTYRAG | 1 cut(s) | 1481 |
| SmoI | CTYRAG | 1 cut(s) | 1481 |
| Sse9I | AATT | 8 cut(s) | 19, 138, 199, 504, 726, 1029, 1451, 1548 |
| SsiI | CCGC | 2 cut(s) | 1492, 1526 |
| SspI | AATATT | 1 cut(s) | 697 |
| SspMI | CTAG | 1 cut(s) | 207 |
| SstI | GAGCTC | 1 cut(s) | 167 |
| StyD4I | CCNGG | 2 cut(s) | 148, 1279 |
| StyI | CCWWGG | 2 cut(s) | 1069, 1256 |
| TaaI | ACNGT | 7 cut(s) | 109, 448, 559, 670, 677, 1225, 1310 |
| TaiI | ACGT | 1 cut(s) | 477 |
| TaqI | TCGA | 3 cut(s) | 386, 608, 941 |
| TaqII | GACCGA | 2 cut(s) | 436, 658 |
| TasI | AATT | 8 cut(s) | 19, 138, 199, 504, 726, 1029, 1451, 1548 |
| TatI | WGTACW | 2 cut(s) | 278, 344 |
| TscAI | CASTG | 3 cut(s) | 150, 682, 1566 |
| TseFI | GTSAC | 1 cut(s) | 1494 |
| TseI | GCWGC | 7 cut(s) | 858, 1191, 1265, 1268, 1367, 1432, 1489 |
| Tsp45I | GTSAC | 1 cut(s) | 1494 |
| TspRI | CASTG | 3 cut(s) | 150, 682, 1566 |
| Tth111I | GACNNNGTC | 1 cut(s) | 332 |
| VpaK11BI | GGWCC | 7 cut(s) | 419, 641, 752, 974, 1085, 1216, 1456 |
| VspI | ATTAAT | 5 cut(s) | 366, 699, 810, 921, 1032 |
| XapI | RAATTY | 2 cut(s) | 19, 138 |
| XceI | RCATGY | 1 cut(s) | 1526 |
| XspI | CTAG | 1 cut(s) | 207 |
| ZrmI | AGTACT | 2 cut(s) | 280, 346 |