Belongs to the helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 634 |
| AccI | GTMKAC | 1 cut(s) | 999 |
| AciI | CCGC | 1 cut(s) | 1034 |
| AclWI | GGATC | 4 cut(s) | 809, 822, 1271, 1424 |
| AcsI | RAATTY | 3 cut(s) | 336, 365, 978 |
| AcuI | CTGAAG | 1 cut(s) | 683 |
| AfaI | GTAC | 4 cut(s) | 419, 441, 614, 692 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1225 |
| AjnI | CCWGG | 3 cut(s) | 615, 771, 1432 |
| AluBI | AGCT | 5 cut(s) | 80, 1174, 1198, 1304, 1408 |
| AluI | AGCT | 5 cut(s) | 80, 1174, 1198, 1304, 1408 |
| Alw26I | GTCTC | 3 cut(s) | 116, 446, 674 |
| AlwI | GGATC | 4 cut(s) | 809, 822, 1271, 1424 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 797, 1280 |
| AoxI | GGCC | 1 cut(s) | 1153 |
| ApeKI | GCWGC | 3 cut(s) | 954, 1031, 1304 |
| ApoI | RAATTY | 3 cut(s) | 336, 365, 978 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1077, 1109 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 565, 981 |
| AsuHPI | GGTGA | 2 cut(s) | 152, 1205 |
| BamHI | GGATCC | 1 cut(s) | 814 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1110, 1142 |
| BbvI | GCAGC | 3 cut(s) | 941, 1043, 1291 |
| BccI | CCATC | 4 cut(s) | 101, 577, 640, 1184 |
| BciT130I | CCWGG | 3 cut(s) | 617, 773, 1434 |
| BclI | TGATCA | 3 cut(s) | 268, 370, 1018 |
| BcoDI | GTCTC | 3 cut(s) | 116, 446, 674 |
| BfaI | CTAG | 2 cut(s) | 534, 1356 |
| BfmI | CTRYAG | 1 cut(s) | 1236 |
| BfuAI | ACCTGC | 1 cut(s) | 634 |
| BglII | AGATCT | 1 cut(s) | 412 |
| BisI | GCNGC | 3 cut(s) | 955, 1032, 1305 |
| BlsI | GCNGC | 3 cut(s) | 956, 1033, 1306 |
| Bme1390I | CCNGG | 3 cut(s) | 617, 773, 1434 |
| BmiI | GGNNCC | 2 cut(s) | 27, 816 |
| BmrFI | CCNGG | 3 cut(s) | 617, 773, 1434 |
| BoxI | GACNNNNGTC | 1 cut(s) | 458 |
| BpuEI | CTTGAG | 2 cut(s) | 526, 1160 |
| BsaI | GGTCTC | 1 cut(s) | 116 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1225 |
| Bse1I | ACTGG | 3 cut(s) | 575, 594, 717 |
| Bse3DI | GCAATG | 2 cut(s) | 303, 856 |
| BseBI | CCWGG | 3 cut(s) | 617, 773, 1434 |
| BseGI | GGATG | 3 cut(s) | 10, 109, 1192 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1225 |
| BseMI | GCAATG | 2 cut(s) | 303, 856 |
| BseNI | ACTGG | 3 cut(s) | 575, 594, 717 |
| BseXI | GCAGC | 3 cut(s) | 941, 1043, 1291 |
| BseYI | CCCAGC | 2 cut(s) | 1258, 1307 |
| BshFI | GGCC | 1 cut(s) | 1155 |
| BslFI | GGGAC | 1 cut(s) | 1324 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1225 |
| BsmAI | GTCTC | 3 cut(s) | 116, 446, 674 |
| BsmFI | GGGAC | 1 cut(s) | 1324 |
| BsnI | GGCC | 1 cut(s) | 1155 |
| Bso31I | GGTCTC | 1 cut(s) | 116 |
| Bsp143I | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| BspACI | CCGC | 1 cut(s) | 1034 |
| BspANI | GGCC | 1 cut(s) | 1155 |
| BspHI | TCATGA | 1 cut(s) | 16 |
| BspLI | GGNNCC | 2 cut(s) | 27, 816 |
| BspMI | ACCTGC | 1 cut(s) | 634 |
| BspPI | GGATC | 4 cut(s) | 809, 822, 1271, 1424 |
| BspTNI | GGTCTC | 1 cut(s) | 116 |
| BsrDI | GCAATG | 2 cut(s) | 303, 856 |
| BsrI | ACTGG | 3 cut(s) | 575, 594, 717 |
| BssMI | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| Bst2UI | CCWGG | 3 cut(s) | 617, 773, 1434 |
| Bst4CI | ACNGT | 6 cut(s) | 422, 460, 793, 884, 1068, 1425 |
| Bst6I | CTCTTC | 2 cut(s) | 1128, 1354 |
| BstC8I | GCNNGC | 1 cut(s) | 385 |
| BstDEI | CTNAG | 1 cut(s) | 81 |
| BstF5I | GGATG | 3 cut(s) | 10, 109, 1192 |
| BstKTI | GATC | 9 cut(s) | 51, 271, 373, 415, 817, 1021, 1106, 1279, 1432 |
| BstMAI | GTCTC | 3 cut(s) | 116, 446, 674 |
| BstMBI | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 152, 906 |
| BstNI | CCWGG | 3 cut(s) | 617, 773, 1434 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 458 |
| BstSCI | CCNGG | 3 cut(s) | 615, 771, 1432 |
| BstSFI | CTRYAG | 1 cut(s) | 1236 |
| BstV1I | GCAGC | 3 cut(s) | 941, 1043, 1291 |
| BstX2I | RGATCY | 3 cut(s) | 412, 814, 1276 |
| BstYI | RGATCY | 3 cut(s) | 412, 814, 1276 |
| BsuRI | GGCC | 1 cut(s) | 1155 |
| BtsCI | GGATG | 3 cut(s) | 10, 109, 1192 |
| BtsIMutI | CAGTG | 2 cut(s) | 582, 798 |
| BveI | ACCTGC | 1 cut(s) | 634 |
| Cac8I | GCNNGC | 1 cut(s) | 385 |
| CaiI | CAGNNNCTG | 2 cut(s) | 797, 1280 |
| CciI | TCATGA | 1 cut(s) | 16 |
| CseI | GACGC | 2 cut(s) | 152, 1037 |
| Csp6I | GTAC | 4 cut(s) | 418, 440, 613, 691 |
| CviQI | GTAC | 4 cut(s) | 418, 440, 613, 691 |
| DdeI | CTNAG | 1 cut(s) | 81 |
| DpnI | GATC | 9 cut(s) | 50, 270, 372, 414, 816, 1020, 1105, 1278, 1431 |
| DpnII | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| DraI | TTTAAA | 1 cut(s) | 600 |
| Eam1104I | CTCTTC | 2 cut(s) | 1128, 1354 |
| EarI | CTCTTC | 2 cut(s) | 1128, 1354 |
| Eco31I | GGTCTC | 1 cut(s) | 116 |
| Eco32I | GATATC | 1 cut(s) | 1213 |
| Eco57I | CTGAAG | 1 cut(s) | 683 |
| EcoRII | CCWGG | 3 cut(s) | 615, 771, 1432 |
| EcoRV | GATATC | 1 cut(s) | 1213 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 966, 998 |
| FaqI | GGGAC | 1 cut(s) | 1324 |
| FauNDI | CATATG | 1 cut(s) | 1224 |
| FbaI | TGATCA | 3 cut(s) | 268, 370, 1018 |
| FblI | GTMKAC | 1 cut(s) | 999 |
| Fnu4HI | GCNGC | 3 cut(s) | 955, 1032, 1305 |
| FokI | GGATG | 3 cut(s) | 17, 116, 1199 |
| Fsp4HI | GCNGC | 3 cut(s) | 955, 1032, 1305 |
| FspBI | CTAG | 2 cut(s) | 534, 1356 |
| GluI | GCNGC | 3 cut(s) | 955, 1032, 1305 |
| GsaI | CCCAGC | 2 cut(s) | 1262, 1311 |
| HaeIII | GGCC | 1 cut(s) | 1155 |
| HgaI | GACGC | 2 cut(s) | 152, 1037 |
| HincII | GTYRAC | 1 cut(s) | 544 |
| HindII | GTYRAC | 1 cut(s) | 544 |
| HinfI | GANTC | 7 cut(s) | 167, 262, 277, 351, 473, 996, 1229 |
| HphI | GGTGA | 2 cut(s) | 152, 1205 |
| Hpy166II | GTNNAC | 3 cut(s) | 187, 544, 1000 |
| Hpy188I | TCNGA | 7 cut(s) | 40, 53, 172, 276, 763, 995, 1339 |
| Hpy8I | GTNNAC | 3 cut(s) | 187, 544, 1000 |
| HpyAV | CCTTC | 2 cut(s) | 314, 1360 |
| HpyCH4III | ACNGT | 6 cut(s) | 422, 460, 793, 884, 1068, 1425 |
| HpyCH4V | TGCA | 6 cut(s) | 155, 296, 727, 849, 872, 1083 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 152, 906 |
| HpyF3I | CTNAG | 1 cut(s) | 81 |
| Ksp22I | TGATCA | 3 cut(s) | 268, 370, 1018 |
| Kzo9I | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| LmnI | GCTCC | 1 cut(s) | 634 |
| Lsp1109I | GCAGC | 3 cut(s) | 941, 1043, 1291 |
| MaeI | CTAG | 2 cut(s) | 534, 1356 |
| MaeIII | GTNAC | 2 cut(s) | 140, 793 |
| MalI | GATC | 9 cut(s) | 50, 270, 372, 414, 816, 1020, 1105, 1278, 1431 |
| MboI | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| MboII | GAAGA | 6 cut(s) | 223, 776, 974, 1145, 1207, 1371 |
| MfeI | CAATTG | 1 cut(s) | 435 |
| MflI | RGATCY | 3 cut(s) | 412, 814, 1276 |
| MlyI | GAGTC | 3 cut(s) | 286, 1005, 1238 |
| MmeI | TCCRAC | 2 cut(s) | 223, 761 |
| MnlI | CCTC | 5 cut(s) | 493, 915, 1145, 1243, 1371 |
| MroXI | GAANNNNTTC | 2 cut(s) | 565, 981 |
| MseI | TTAA | 3 cut(s) | 590, 599, 923 |
| MspA1I | CMGCKG | 2 cut(s) | 1034, 1408 |
| MspR9I | CCNGG | 3 cut(s) | 617, 773, 1434 |
| MunI | CAATTG | 1 cut(s) | 435 |
| MvaI | CCWGG | 3 cut(s) | 617, 773, 1434 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 152, 906 |
| NdeI | CATATG | 1 cut(s) | 1224 |
| NdeII | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| NlaIV | GGNNCC | 2 cut(s) | 27, 816 |
| NmeAIII | GCCGAG | 1 cut(s) | 930 |
| NmuCI | GTSAC | 2 cut(s) | 140, 793 |
| PagI | TCATGA | 1 cut(s) | 16 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 913 |
| PdmI | GAANNNNTTC | 2 cut(s) | 565, 981 |
| PfeI | GAWTC | 4 cut(s) | 167, 262, 351, 473 |
| PfoI | TCCNGGA | 2 cut(s) | 771, 1432 |
| PkrI | GCNGC | 3 cut(s) | 956, 1033, 1306 |
| PleI | GAGTC | 3 cut(s) | 285, 1004, 1237 |
| PpsI | GAGTC | 3 cut(s) | 285, 1004, 1237 |
| PshAI | GACNNNNGTC | 1 cut(s) | 458 |
| Psp6I | CCWGG | 3 cut(s) | 615, 771, 1432 |
| PspFI | CCCAGC | 2 cut(s) | 1258, 1307 |
| PspGI | CCWGG | 3 cut(s) | 615, 771, 1432 |
| PspN4I | GGNNCC | 2 cut(s) | 27, 816 |
| PstNI | CAGNNNCTG | 2 cut(s) | 797, 1280 |
| PsuI | RGATCY | 3 cut(s) | 412, 814, 1276 |
| PvuII | CAGCTG | 1 cut(s) | 1408 |
| RsaI | GTAC | 4 cut(s) | 419, 441, 614, 692 |
| RsaNI | GTAC | 4 cut(s) | 418, 440, 613, 691 |
| SaqAI | TTAA | 3 cut(s) | 590, 599, 923 |
| SatI | GCNGC | 3 cut(s) | 955, 1032, 1305 |
| Sau3AI | GATC | 9 cut(s) | 48, 268, 370, 412, 814, 1018, 1103, 1276, 1429 |
| SchI | GAGTC | 3 cut(s) | 286, 1005, 1238 |
| ScrFI | CCNGG | 3 cut(s) | 617, 773, 1434 |
| SfcI | CTRYAG | 1 cut(s) | 1236 |
| SmlI | CTYRAG | 2 cut(s) | 505, 1175 |
| SmoI | CTYRAG | 2 cut(s) | 505, 1175 |
| SsiI | CCGC | 1 cut(s) | 1034 |
| SspMI | CTAG | 2 cut(s) | 534, 1356 |
| StyD4I | CCNGG | 3 cut(s) | 615, 771, 1432 |
| TaaI | ACNGT | 6 cut(s) | 422, 460, 793, 884, 1068, 1425 |
| TaqI | TCGA | 2 cut(s) | 65, 916 |
| TatI | WGTACW | 1 cut(s) | 690 |
| TfiI | GAWTC | 4 cut(s) | 167, 262, 351, 473 |
| Tru1I | TTAA | 3 cut(s) | 590, 599, 923 |
| Tru9I | TTAA | 3 cut(s) | 590, 599, 923 |
| TscAI | CASTG | 2 cut(s) | 582, 798 |
| TseFI | GTSAC | 2 cut(s) | 140, 793 |
| TseI | GCWGC | 3 cut(s) | 954, 1031, 1304 |
| Tsp45I | GTSAC | 2 cut(s) | 140, 793 |
| TspGWI | ACGGA | 1 cut(s) | 275 |
| TspRI | CASTG | 2 cut(s) | 582, 798 |
| XapI | RAATTY | 3 cut(s) | 336, 365, 978 |
| XbaI | TCTAGA | 1 cut(s) | 1355 |
| XmiI | GTMKAC | 1 cut(s) | 999 |
| XmnI | GAANNNNTTC | 2 cut(s) | 565, 981 |
| XspI | CTAG | 2 cut(s) | 534, 1356 |