DNA recombination
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 616 |
| AclWI | GGATC | 3 cut(s) | 649, 791, 804 |
| AcsI | RAATTY | 2 cut(s) | 366, 395 |
| AcuI | CTGAAG | 1 cut(s) | 665 |
| AfaI | GTAC | 2 cut(s) | 596, 674 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 82 |
| AgsI | TTSAA | 9 cut(s) | 40, 322, 341, 350, 460, 601, 730, 787, 848 |
| AjnI | CCWGG | 2 cut(s) | 75, 753 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 44, 76 |
| AluBI | AGCT | 4 cut(s) | 44, 98, 119, 548 |
| AluI | AGCT | 4 cut(s) | 44, 98, 119, 548 |
| Alw26I | GTCTC | 1 cut(s) | 257 |
| AlwI | GGATC | 3 cut(s) | 649, 791, 804 |
| ApoI | RAATTY | 2 cut(s) | 366, 395 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 132, 164 |
| AseI | ATTAAT | 1 cut(s) | 468 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 547 |
| AsuII | TTCGAA | 1 cut(s) | 25 |
| BamHI | GGATCC | 1 cut(s) | 796 |
| BbsI | GAAGAC | 1 cut(s) | 142 |
| BccI | CCATC | 3 cut(s) | 242, 559, 622 |
| BciT130I | CCWGG | 2 cut(s) | 77, 755 |
| BclI | TGATCA | 3 cut(s) | 298, 400, 424 |
| BcoDI | GTCTC | 1 cut(s) | 257 |
| BfaI | CTAG | 1 cut(s) | 516 |
| BfuAI | ACCTGC | 1 cut(s) | 616 |
| BmcAI | AGTACT | 1 cut(s) | 596 |
| Bme1390I | CCNGG | 2 cut(s) | 77, 755 |
| BmiI | GGNNCC | 2 cut(s) | 168, 798 |
| BmrFI | CCNGG | 2 cut(s) | 77, 755 |
| BmsI | GCATC | 1 cut(s) | 385 |
| BpiI | GAAGAC | 1 cut(s) | 142 |
| Bpu14I | TTCGAA | 1 cut(s) | 25 |
| BpuEI | CTTGAG | 1 cut(s) | 508 |
| Bsa29I | ATCGAT | 1 cut(s) | 379 |
| BsaBI | GATNNNNATC | 1 cut(s) | 15 |
| BsaI | GGTCTC | 1 cut(s) | 257 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 82 |
| Bse1I | ACTGG | 3 cut(s) | 112, 557, 699 |
| Bse8I | GATNNNNATC | 1 cut(s) | 15 |
| BseBI | CCWGG | 2 cut(s) | 77, 755 |
| BseCI | ATCGAT | 1 cut(s) | 379 |
| BseGI | GGATG | 2 cut(s) | 72, 151 |
| BseJI | GATNNNNATC | 1 cut(s) | 15 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 82 |
| BseNI | ACTGG | 3 cut(s) | 112, 557, 699 |
| BshVI | ATCGAT | 1 cut(s) | 379 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 82 |
| BsmAI | GTCTC | 1 cut(s) | 257 |
| Bso31I | GGTCTC | 1 cut(s) | 257 |
| Bsp119I | TTCGAA | 1 cut(s) | 25 |
| Bsp143I | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| BspDI | ATCGAT | 1 cut(s) | 379 |
| BspHI | TCATGA | 1 cut(s) | 157 |
| BspLI | GGNNCC | 2 cut(s) | 168, 798 |
| BspMI | ACCTGC | 1 cut(s) | 616 |
| BspPI | GGATC | 3 cut(s) | 649, 791, 804 |
| BspT104I | TTCGAA | 1 cut(s) | 25 |
| BspTNI | GGTCTC | 1 cut(s) | 257 |
| BsrI | ACTGG | 3 cut(s) | 112, 557, 699 |
| BssMI | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| Bst2UI | CCWGG | 2 cut(s) | 77, 755 |
| Bst4CI | ACNGT | 3 cut(s) | 334, 775, 862 |
| Bst6I | CTCTTC | 1 cut(s) | 472 |
| BstBI | TTCGAA | 1 cut(s) | 25 |
| BstC8I | GCNNGC | 1 cut(s) | 415 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 80 |
| BstF5I | GGATG | 2 cut(s) | 72, 151 |
| BstKTI | GATC | 6 cut(s) | 192, 301, 403, 427, 657, 799 |
| BstMAI | GTCTC | 1 cut(s) | 257 |
| BstMBI | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| BstNI | CCWGG | 2 cut(s) | 77, 755 |
| BstSCI | CCNGG | 2 cut(s) | 75, 753 |
| BstV2I | GAAGAC | 1 cut(s) | 142 |
| BstX2I | RGATCY | 2 cut(s) | 654, 796 |
| BstYI | RGATCY | 2 cut(s) | 654, 796 |
| Bsu15I | ATCGAT | 1 cut(s) | 379 |
| BsuTUI | ATCGAT | 1 cut(s) | 379 |
| BtsCI | GGATG | 2 cut(s) | 72, 151 |
| BtsI | GCAGTG | 1 cut(s) | 510 |
| BtsIMutI | CAGTG | 4 cut(s) | 326, 510, 564, 780 |
| BveI | ACCTGC | 1 cut(s) | 616 |
| Cac8I | GCNNGC | 1 cut(s) | 415 |
| CciI | TCATGA | 1 cut(s) | 157 |
| ClaI | ATCGAT | 1 cut(s) | 379 |
| Csp6I | GTAC | 2 cut(s) | 595, 673 |
| CviAII | CATG | 4 cut(s) | 90, 158, 418, 529 |
| CviJI | RGCY | 8 cut(s) | 44, 98, 111, 119, 453, 548, 679, 736 |
| CviKI_1 | RGCY | 8 cut(s) | 44, 98, 111, 119, 453, 548, 679, 736 |
| CviQI | GTAC | 2 cut(s) | 595, 673 |
| DpnI | GATC | 6 cut(s) | 191, 300, 402, 426, 656, 798 |
| DpnII | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| DraI | TTTAAA | 1 cut(s) | 582 |
| Eam1104I | CTCTTC | 1 cut(s) | 472 |
| EarI | CTCTTC | 1 cut(s) | 472 |
| Eco31I | GGTCTC | 1 cut(s) | 257 |
| Eco57I | CTGAAG | 1 cut(s) | 665 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 80 |
| EcoRII | CCWGG | 2 cut(s) | 75, 753 |
| FaeI | CATG | 4 cut(s) | 93, 161, 421, 532 |
| FatI | CATG | 4 cut(s) | 89, 157, 417, 528 |
| FbaI | TGATCA | 3 cut(s) | 298, 400, 424 |
| FokI | GGATG | 2 cut(s) | 59, 158 |
| FspBI | CTAG | 1 cut(s) | 516 |
| Hin1II | CATG | 4 cut(s) | 93, 161, 421, 532 |
| HincII | GTYRAC | 1 cut(s) | 526 |
| HindII | GTYRAC | 1 cut(s) | 526 |
| HindIII | AAGCTT | 1 cut(s) | 546 |
| HinfI | GANTC | 4 cut(s) | 292, 307, 381, 778 |
| Hpy166II | GTNNAC | 1 cut(s) | 526 |
| Hpy188I | TCNGA | 4 cut(s) | 181, 194, 306, 745 |
| Hpy188III | TCNNGA | 5 cut(s) | 158, 207, 265, 296, 371 |
| Hpy8I | GTNNAC | 1 cut(s) | 526 |
| HpyAV | CCTTC | 1 cut(s) | 344 |
| HpyCH4III | ACNGT | 3 cut(s) | 334, 775, 862 |
| HpyCH4V | TGCA | 2 cut(s) | 326, 709 |
| Hsp92II | CATG | 4 cut(s) | 93, 161, 421, 532 |
| Ksp22I | TGATCA | 3 cut(s) | 298, 400, 424 |
| Kzo9I | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| LweI | GCATC | 1 cut(s) | 385 |
| MaeI | CTAG | 1 cut(s) | 516 |
| MaeIII | GTNAC | 1 cut(s) | 775 |
| MalI | GATC | 6 cut(s) | 191, 300, 402, 426, 656, 798 |
| MboI | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| MboII | GAAGA | 4 cut(s) | 142, 489, 758, 833 |
| MflI | RGATCY | 2 cut(s) | 654, 796 |
| MluCI | AATT | 7 cut(s) | 52, 366, 395, 432, 469, 492, 537 |
| MlyI | GAGTC | 2 cut(s) | 316, 772 |
| MmeI | TCCRAC | 1 cut(s) | 743 |
| MnlI | CCTC | 2 cut(s) | 475, 811 |
| MroXI | GAANNNNTTC | 1 cut(s) | 547 |
| MseI | TTAA | 6 cut(s) | 68, 468, 473, 572, 581, 865 |
| MspR9I | CCNGG | 2 cut(s) | 77, 755 |
| MvaI | CCWGG | 2 cut(s) | 77, 755 |
| NdeII | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| NlaIII | CATG | 4 cut(s) | 93, 161, 421, 532 |
| NlaIV | GGNNCC | 2 cut(s) | 168, 798 |
| NmuCI | GTSAC | 1 cut(s) | 775 |
| NspV | TTCGAA | 1 cut(s) | 25 |
| PagI | TCATGA | 1 cut(s) | 157 |
| PdmI | GAANNNNTTC | 1 cut(s) | 547 |
| PfeI | GAWTC | 2 cut(s) | 292, 381 |
| PfoI | TCCNGGA | 1 cut(s) | 753 |
| PleI | GAGTC | 2 cut(s) | 315, 772 |
| PpsI | GAGTC | 2 cut(s) | 315, 772 |
| PshBI | ATTAAT | 1 cut(s) | 468 |
| Psp6I | CCWGG | 2 cut(s) | 75, 753 |
| PspGI | CCWGG | 2 cut(s) | 75, 753 |
| PspN4I | GGNNCC | 2 cut(s) | 168, 798 |
| PsuI | RGATCY | 2 cut(s) | 654, 796 |
| RsaI | GTAC | 2 cut(s) | 596, 674 |
| RsaNI | GTAC | 2 cut(s) | 595, 673 |
| SaqAI | TTAA | 6 cut(s) | 68, 468, 473, 572, 581, 865 |
| Sau3AI | GATC | 6 cut(s) | 189, 298, 400, 424, 654, 796 |
| ScaI | AGTACT | 1 cut(s) | 596 |
| SchI | GAGTC | 2 cut(s) | 316, 772 |
| ScrFI | CCNGG | 2 cut(s) | 77, 755 |
| SetI | ASST | 8 cut(s) | 46, 87, 100, 121, 172, 550, 610, 635 |
| SfaNI | GCATC | 1 cut(s) | 385 |
| SfuI | TTCGAA | 1 cut(s) | 25 |
| SmlI | CTYRAG | 1 cut(s) | 487 |
| SmoI | CTYRAG | 1 cut(s) | 487 |
| Sse9I | AATT | 7 cut(s) | 52, 366, 395, 432, 469, 492, 537 |
| SspMI | CTAG | 1 cut(s) | 516 |
| StyD4I | CCNGG | 2 cut(s) | 75, 753 |
| TaaI | ACNGT | 3 cut(s) | 334, 775, 862 |
| TaqI | TCGA | 7 cut(s) | 19, 25, 206, 379, 408, 464, 781 |
| TasI | AATT | 7 cut(s) | 52, 366, 395, 432, 469, 492, 537 |
| TatI | WGTACW | 2 cut(s) | 594, 672 |
| TfiI | GAWTC | 2 cut(s) | 292, 381 |
| Tru1I | TTAA | 6 cut(s) | 68, 468, 473, 572, 581, 865 |
| Tru9I | TTAA | 6 cut(s) | 68, 468, 473, 572, 581, 865 |
| TscAI | CASTG | 4 cut(s) | 333, 517, 564, 780 |
| TseFI | GTSAC | 1 cut(s) | 775 |
| Tsp45I | GTSAC | 1 cut(s) | 775 |
| TspGWI | ACGGA | 1 cut(s) | 305 |
| TspRI | CASTG | 4 cut(s) | 333, 517, 564, 780 |
| VspI | ATTAAT | 1 cut(s) | 468 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 80 |
| XapI | RAATTY | 2 cut(s) | 366, 395 |
| XmnI | GAANNNNTTC | 1 cut(s) | 547 |
| XspI | CTAG | 1 cut(s) | 516 |
| ZrmI | AGTACT | 1 cut(s) | 596 |