Belongs to the helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 988, 1405 |
| AccI | GTMKAC | 1 cut(s) | 650 |
| AccII | CGCG | 1 cut(s) | 1350 |
| AciI | CCGC | 5 cut(s) | 23, 1145, 1350, 1449, 1493 |
| AclWI | GGATC | 4 cut(s) | 547, 707, 1007, 1020 |
| AcsI | RAATTY | 4 cut(s) | 104, 764, 1042, 1394 |
| AcuI | CTGAAG | 1 cut(s) | 345 |
| AfaI | GTAC | 2 cut(s) | 459, 791 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 527, 885, 988, 1199, 1405 |
| AgsI | TTSAA | 8 cut(s) | 164, 169, 232, 559, 623, 764, 1296, 1424 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1228, 1260 |
| AleI | CACNNNNGTG | 1 cut(s) | 989 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 507, 539, 873, 905 |
| AluBI | AGCT | 6 cut(s) | 155, 244, 283, 641, 1034, 1072 |
| AluI | AGCT | 6 cut(s) | 155, 244, 283, 641, 1034, 1072 |
| Alw26I | GTCTC | 4 cut(s) | 42, 384, 416, 434 |
| AlwI | GGATC | 4 cut(s) | 547, 707, 1007, 1020 |
| AoxI | GGCC | 1 cut(s) | 655 |
| ApeKI | GCWGC | 2 cut(s) | 152, 316 |
| ApoI | RAATTY | 4 cut(s) | 104, 764, 1042, 1394 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 96, 128 |
| AspS9I | GGNCC | 2 cut(s) | 67, 655 |
| AsuHPI | GGTGA | 3 cut(s) | 606, 1003, 1241 |
| AvaII | GGWCC | 1 cut(s) | 67 |
| BamHI | GGATCC | 1 cut(s) | 1012 |
| BanII | GRGCYC | 1 cut(s) | 1157 |
| BbsI | GAAGAC | 1 cut(s) | 955 |
| BbvCI | CCTCAGC | 1 cut(s) | 637 |
| BbvI | GCAGC | 2 cut(s) | 164, 328 |
| BccI | CCATC | 3 cut(s) | 44, 78, 995 |
| BceAI | ACGGC | 2 cut(s) | 324, 642 |
| BcoDI | GTCTC | 4 cut(s) | 42, 384, 416, 434 |
| BglII | AGATCT | 1 cut(s) | 898 |
| BisI | GCNGC | 4 cut(s) | 153, 317, 1351, 1449 |
| BlsI | GCNGC | 4 cut(s) | 154, 318, 1352, 1450 |
| BmcAI | AGTACT | 1 cut(s) | 459 |
| Bme18I | GGWCC | 1 cut(s) | 67 |
| BmgT120I | GGNCC | 2 cut(s) | 67, 655 |
| BmiI | GGNNCC | 3 cut(s) | 34, 890, 1014 |
| BmrI | ACTGGG | 1 cut(s) | 1264 |
| BmsI | GCATC | 1 cut(s) | 1230 |
| BmuI | ACTGGG | 1 cut(s) | 1264 |
| BpiI | GAAGAC | 1 cut(s) | 955 |
| Bpu10I | CCTNAGC | 1 cut(s) | 637 |
| BpuEI | CTTGAG | 1 cut(s) | 1203 |
| BsaI | GGTCTC | 1 cut(s) | 434 |
| BsaJI | CCNNGG | 3 cut(s) | 511, 884, 1167 |
| BsaWI | WCCGGW | 1 cut(s) | 1321 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1174, 1204 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 527, 885, 988, 1199, 1405 |
| Bse1I | ACTGG | 3 cut(s) | 230, 444, 1270 |
| Bse3DI | GCAATG | 2 cut(s) | 91, 856 |
| BseDI | CCNNGG | 3 cut(s) | 511, 884, 1167 |
| BseGI | GGATG | 2 cut(s) | 51, 1006 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 527, 885, 988, 1199, 1405 |
| BseMI | GCAATG | 2 cut(s) | 91, 856 |
| BseMII | CTCAG | 5 cut(s) | 633, 651, 669, 1468, 1523 |
| BseNI | ACTGG | 3 cut(s) | 230, 444, 1270 |
| BseXI | GCAGC | 2 cut(s) | 164, 328 |
| BseYI | CCCAGC | 1 cut(s) | 1229 |
| BsgI | GTGCAG | 2 cut(s) | 335, 681 |
| Bsh1236I | CGCG | 1 cut(s) | 1350 |
| Bsh1285I | CGRYCG | 1 cut(s) | 46 |
| BshFI | GGCC | 1 cut(s) | 657 |
| BsiEI | CGRYCG | 1 cut(s) | 46 |
| BsiSI | CCGG | 1 cut(s) | 1322 |
| BslFI | GGGAC | 2 cut(s) | 128, 1397 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 527, 885, 988, 1199, 1405 |
| BsmAI | GTCTC | 4 cut(s) | 42, 384, 416, 434 |
| BsmBI | CGTCTC | 1 cut(s) | 384 |
| BsmFI | GGGAC | 2 cut(s) | 128, 1397 |
| BsmI | GAATGC | 1 cut(s) | 736 |
| BsnI | GGCC | 1 cut(s) | 657 |
| Bso31I | GGTCTC | 1 cut(s) | 434 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1157 |
| Bsp143I | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| Bsp19I | CCATGG | 1 cut(s) | 884 |
| BspACI | CCGC | 5 cut(s) | 23, 1145, 1350, 1449, 1493 |
| BspANI | GGCC | 1 cut(s) | 657 |
| BspCNI | CTCAG | 5 cut(s) | 634, 650, 670, 1467, 1522 |
| BspFNI | CGCG | 1 cut(s) | 1350 |
| BspHI | TCATGA | 1 cut(s) | 939 |
| BspLI | GGNNCC | 3 cut(s) | 34, 890, 1014 |
| BspPI | GGATC | 4 cut(s) | 547, 707, 1007, 1020 |
| BspTNI | GGTCTC | 1 cut(s) | 434 |
| BsrDI | GCAATG | 2 cut(s) | 91, 856 |
| BsrI | ACTGG | 3 cut(s) | 230, 444, 1270 |
| BssECI | CCNNGG | 3 cut(s) | 511, 884, 1167 |
| BssMI | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| BssNAI | GTATAC | 1 cut(s) | 651 |
| BssT1I | CCWWGG | 3 cut(s) | 511, 884, 1167 |
| Bst1107I | GTATAC | 1 cut(s) | 651 |
| Bst4CI | ACNGT | 2 cut(s) | 44, 400 |
| Bst6I | CTCTTC | 2 cut(s) | 356, 624 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 668 |
| BstDEI | CTNAG | 6 cut(s) | 74, 637, 642, 678, 1454, 1509 |
| BstDSI | CCRYGG | 1 cut(s) | 884 |
| BstF5I | GGATG | 2 cut(s) | 51, 1006 |
| BstFNI | CGCG | 1 cut(s) | 1350 |
| BstKTI | GATC | 7 cut(s) | 131, 542, 614, 715, 901, 1015, 1141 |
| BstMAI | GTCTC | 4 cut(s) | 42, 384, 416, 434 |
| BstMBI | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| BstMCI | CGRYCG | 1 cut(s) | 46 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 668, 807, 1385, 1457 |
| BstNSI | RCATGY | 1 cut(s) | 956 |
| BstUI | CGCG | 1 cut(s) | 1350 |
| BstV1I | GCAGC | 2 cut(s) | 164, 328 |
| BstV2I | GAAGAC | 1 cut(s) | 955 |
| BstX2I | RGATCY | 2 cut(s) | 898, 1012 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 225 |
| BstYI | RGATCY | 2 cut(s) | 898, 1012 |
| BstZ17I | GTATAC | 1 cut(s) | 651 |
| BsuRI | GGCC | 1 cut(s) | 657 |
| BtgI | CCRYGG | 1 cut(s) | 884 |
| BtsCI | GGATG | 2 cut(s) | 51, 1006 |
| BtsIMutI | CAGTG | 5 cut(s) | 223, 405, 451, 673, 1092 |
| CciI | TCATGA | 1 cut(s) | 939 |
| Cfr13I | GGNCC | 2 cut(s) | 67, 655 |
| Csp6I | GTAC | 2 cut(s) | 458, 790 |
| CviAII | CATG | 8 cut(s) | 149, 798, 857, 885, 940, 953, 1007, 1124 |
| CviQI | GTAC | 2 cut(s) | 458, 790 |
| DdeI | CTNAG | 6 cut(s) | 74, 637, 642, 678, 1454, 1509 |
| DpnI | GATC | 7 cut(s) | 130, 541, 613, 714, 900, 1014, 1140 |
| DpnII | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| Eam1104I | CTCTTC | 2 cut(s) | 356, 624 |
| EarI | CTCTTC | 2 cut(s) | 356, 624 |
| Eco130I | CCWWGG | 3 cut(s) | 511, 884, 1167 |
| Eco24I | GRGCYC | 1 cut(s) | 1157 |
| Eco31I | GGTCTC | 1 cut(s) | 434 |
| Eco47I | GGWCC | 1 cut(s) | 67 |
| Eco57I | CTGAAG | 1 cut(s) | 345 |
| EcoRI | GAATTC | 2 cut(s) | 764, 1394 |
| EcoT14I | CCWWGG | 3 cut(s) | 511, 884, 1167 |
| EcoT22I | ATGCAT | 1 cut(s) | 91 |
| EcoT38I | GRGCYC | 1 cut(s) | 1157 |
| ErhI | CCWWGG | 3 cut(s) | 511, 884, 1167 |
| Esp3I | CGTCTC | 1 cut(s) | 384 |
| FaeI | CATG | 8 cut(s) | 152, 801, 860, 888, 943, 956, 1010, 1127 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1097, 1129 |
| FaqI | GGGAC | 2 cut(s) | 128, 1397 |
| FatI | CATG | 8 cut(s) | 148, 797, 856, 884, 939, 952, 1006, 1123 |
| FauNDI | CATATG | 1 cut(s) | 91 |
| FblI | GTMKAC | 1 cut(s) | 650 |
| Fnu4HI | GCNGC | 4 cut(s) | 153, 317, 1351, 1449 |
| FokI | GGATG | 2 cut(s) | 38, 1013 |
| FriOI | GRGCYC | 1 cut(s) | 1157 |
| Fsp4HI | GCNGC | 4 cut(s) | 153, 317, 1351, 1449 |
| GluI | GCNGC | 4 cut(s) | 153, 317, 1351, 1449 |
| GsaI | CCCAGC | 1 cut(s) | 1233 |
| HaeIII | GGCC | 1 cut(s) | 657 |
| HapII | CCGG | 1 cut(s) | 1322 |
| Hin1II | CATG | 8 cut(s) | 152, 801, 860, 888, 943, 956, 1010, 1127 |
| HincII | GTYRAC | 1 cut(s) | 1521 |
| HindII | GTYRAC | 1 cut(s) | 1521 |
| HinfI | GANTC | 5 cut(s) | 264, 568, 680, 836, 1216 |
| HpaI | GTTAAC | 1 cut(s) | 1521 |
| HpaII | CCGG | 1 cut(s) | 1322 |
| HphI | GGTGA | 3 cut(s) | 606, 1003, 1241 |
| Hpy166II | GTNNAC | 4 cut(s) | 28, 651, 1504, 1521 |
| Hpy188I | TCNGA | 5 cut(s) | 530, 583, 835, 1457, 1512 |
| Hpy188III | TCNNGA | 7 cut(s) | 47, 209, 340, 417, 563, 823, 940 |
| Hpy8I | GTNNAC | 4 cut(s) | 28, 651, 1504, 1521 |
| HpyAV | CCTTC | 1 cut(s) | 1254 |
| HpyCH4III | ACNGT | 2 cut(s) | 44, 400 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 668, 807, 1385, 1457 |
| HpyF3I | CTNAG | 6 cut(s) | 74, 637, 642, 678, 1454, 1509 |
| Hsp92II | CATG | 8 cut(s) | 152, 801, 860, 888, 943, 956, 1010, 1127 |
| KspAI | GTTAAC | 1 cut(s) | 1521 |
| Kzo9I | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| Lsp1109I | GCAGC | 2 cut(s) | 164, 328 |
| LweI | GCATC | 1 cut(s) | 1230 |
| MaeIII | GTNAC | 1 cut(s) | 1171 |
| MalI | GATC | 7 cut(s) | 130, 541, 613, 714, 900, 1014, 1140 |
| MboI | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| MboII | GAAGA | 6 cut(s) | 338, 373, 611, 923, 960, 1508 |
| MfeI | CAATTG | 2 cut(s) | 690, 1161 |
| MflI | RGATCY | 2 cut(s) | 898, 1012 |
| MhlI | GDGCHC | 1 cut(s) | 1157 |
| MlyI | GAGTC | 2 cut(s) | 689, 1210 |
| MnlI | CCTC | 8 cut(s) | 156, 627, 646, 947, 991, 1003, 1204, 1356 |
| Mph1103I | ATGCAT | 1 cut(s) | 91 |
| MseI | TTAA | 5 cut(s) | 18, 236, 246, 600, 1520 |
| MslI | CAYNNNNRTG | 3 cut(s) | 75, 729, 989 |
| MspA1I | CMGCKG | 1 cut(s) | 641 |
| MspI | CCGG | 1 cut(s) | 1322 |
| MunI | CAATTG | 2 cut(s) | 690, 1161 |
| Mva1269I | GAATGC | 1 cut(s) | 736 |
| MvnI | CGCG | 1 cut(s) | 1350 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 668, 807, 1385, 1457 |
| NcoI | CCATGG | 1 cut(s) | 884 |
| NdeI | CATATG | 1 cut(s) | 91 |
| NdeII | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| NlaIII | CATG | 8 cut(s) | 152, 801, 860, 888, 943, 956, 1010, 1127 |
| NlaIV | GGNNCC | 3 cut(s) | 34, 890, 1014 |
| NmuCI | GTSAC | 1 cut(s) | 1171 |
| NsiI | ATGCAT | 1 cut(s) | 91 |
| NspI | RCATGY | 1 cut(s) | 956 |
| OliI | CACNNNNGTG | 1 cut(s) | 989 |
| PagI | TCATGA | 1 cut(s) | 939 |
| PctI | GAATGC | 1 cut(s) | 736 |
| PfeI | GAWTC | 3 cut(s) | 264, 568, 836 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 988, 1405 |
| PkrI | GCNGC | 4 cut(s) | 154, 318, 1352, 1450 |
| PleI | GAGTC | 2 cut(s) | 688, 1210 |
| PpsI | GAGTC | 2 cut(s) | 688, 1210 |
| PspFI | CCCAGC | 1 cut(s) | 1229 |
| PspN4I | GGNNCC | 3 cut(s) | 34, 890, 1014 |
| PspPI | GGNCC | 2 cut(s) | 67, 655 |
| PsuI | RGATCY | 2 cut(s) | 898, 1012 |
| PvuII | CAGCTG | 1 cut(s) | 641 |
| RsaI | GTAC | 2 cut(s) | 459, 791 |
| RsaNI | GTAC | 2 cut(s) | 458, 790 |
| RseI | CAYNNNNRTG | 3 cut(s) | 75, 729, 989 |
| SaqAI | TTAA | 5 cut(s) | 18, 236, 246, 600, 1520 |
| SatI | GCNGC | 4 cut(s) | 153, 317, 1351, 1449 |
| Sau3AI | GATC | 7 cut(s) | 128, 539, 611, 712, 898, 1012, 1138 |
| Sau96I | GGNCC | 2 cut(s) | 67, 655 |
| ScaI | AGTACT | 1 cut(s) | 459 |
| SchI | GAGTC | 2 cut(s) | 689, 1210 |
| SduI | GDGCHC | 1 cut(s) | 1157 |
| SfaNI | GCATC | 1 cut(s) | 1230 |
| SinI | GGWCC | 1 cut(s) | 67 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 75, 729, 989 |
| SmlI | CTYRAG | 1 cut(s) | 1218 |
| SmoI | CTYRAG | 1 cut(s) | 1218 |
| SsiI | CCGC | 5 cut(s) | 23, 1145, 1350, 1449, 1493 |
| StyI | CCWWGG | 3 cut(s) | 511, 884, 1167 |
| TaaI | ACNGT | 2 cut(s) | 44, 400 |
| TaqI | TCGA | 5 cut(s) | 46, 131, 267, 768, 1151 |
| TatI | WGTACW | 1 cut(s) | 457 |
| TauI | GCSGC | 2 cut(s) | 1353, 1451 |
| TfiI | GAWTC | 3 cut(s) | 264, 568, 836 |
| Tru1I | TTAA | 5 cut(s) | 18, 236, 246, 600, 1520 |
| Tru9I | TTAA | 5 cut(s) | 18, 236, 246, 600, 1520 |
| TscAI | CASTG | 5 cut(s) | 230, 405, 451, 673, 1099 |
| TseFI | GTSAC | 1 cut(s) | 1171 |
| TseI | GCWGC | 2 cut(s) | 152, 316 |
| Tsp45I | GTSAC | 1 cut(s) | 1171 |
| TspDTI | ATGAA | 8 cut(s) | 108, 245, 853, 867, 1009, 1105, 1112, 1332 |
| TspGWI | ACGGA | 3 cut(s) | 45, 1186, 1524 |
| TspRI | CASTG | 5 cut(s) | 230, 405, 451, 673, 1099 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 988, 1405 |
| VpaK11BI | GGWCC | 1 cut(s) | 67 |
| XapI | RAATTY | 4 cut(s) | 104, 764, 1042, 1394 |
| XceI | RCATGY | 1 cut(s) | 956 |
| XmiI | GTMKAC | 1 cut(s) | 650 |
| ZrmI | AGTACT | 1 cut(s) | 459 |
| Zsp2I | ATGCAT | 1 cut(s) | 91 |