Rorug01G0127300

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000050
Physical Location & Seq
Forward (+)
21705880 .. 21710905
5026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0127300.1

Sequence Viewer

Length: 1788 bp
ATGGGCCGTCAGTCTTCGCAATCGCTCGCTCCCGGGTTCCGATTCCACCCCACCGATGAAGAACTTGTCTGGTACTACCTGAAGCGCAAGGTCGCCGGAAAATCTTTCCGCTTCGACGCCATCTCCGTCGTCGATATCTACAAGATCGAGCCTTGGGACCTCCCTGGGAAGTCGAAGCTGAAAAGCAGGGACACGGAATGGTACTTCTTCAGTCTTCTGGACAAGAAGTATGGGAATTCGTCGAGGACGAATCGGGCTACCGAAAAAGGGTACTGGAAGACTACTGGGAAGGATCGCCCCGTTTGCCATAATTCCAGGGCGGTGGGGATGAAGAAGACCCTTGTCTTCCACAGTGGCCGAGCCCCCAAAGGTGCCAGGACCAATTGGGTTATGCATGAGTACCGCCTCGATAACCAGGAATTGGAGAAAGCTGGAATAATAGAGAAAGATGCATATGTGCTCTGCAGGATATTCCAGAAGAGTGGTACCGGGCCCAAGAATGGGGAGCAGTATGGTGCTCCATTTATTGAGGAGGAATGGGATGAAGAAGATGAGGTTTATGGACCTGGTGAAGAGGCTATTGCTAATGAAGTAGCAGTCAGCGGTGGGCCGTATACTGAAGCAAATGAGCTCAGCGATGGGGATGGGGGATATGTCGAGGCAATTGACCTTGATCAGAATATTGACACAGGTGTTCCTGAAAGTGGTCCTCGTCCATTGAACTTCTATCATGGGGAGACAAGCAACTATATTGAGCAATCTGGGGATTTTGTTGAAGATGCTGCAAAGACTGCAGTAGGCGCTGGGGAAACTCTGGAATATTGCGAAGAGCAGAAGTACTTCGACTTACCAGAGCAGTATGAGATGGGTGCAAAGCCTGTCAAAGTTGAATACTTAACTTCGGAATACGATGACAACCAGAACTTCTTCAACTTACCAGAGTATTATGAGACAGAAACAAAAGCGGTCAAAGATGAATATGTTGAACCAAGCCATAATATGAATCCTGTGGATGTCAACTACTCACTCAATGATCCATATTTAAATACTACTGAAAACCCGCCATTTGGTGACGGGCAATTCTTAGAAACTAATGATCTTTCAATTCCAGCCAATCCAGTTGAGCCAAATTCTGCCGGCTTTGATATGCTTGATGAGTACCTTGCATACTTTGATGCGGATGATGACATCTCTCAGTACATTGATTTTGAGTCTTGTGGAATGCTGGGGAATGAAAATACTGTTCCTAGTGAACCTCTGGTTGAGCAGTCTGTTATGGGTGAAACTCAGCCACAATCCACAGGAAGCCAACATCTTGTAGAGGCACATGATGCCAATGATGCATCTTCATCAAAACAAAAGCCGGACGTGAAATTTGGATCAGATGGCAAGTTTCCATTCATCACAAAGGCGAGTCAGATGTTGGGCAGTATACCTGCTCCTCCTGCATTTGCTTCAGAGTTCCCTCCTAAAGATGCAATCCTTCGGTTGAATTCTGAAGTAGCATCCTCAAGTTCAGTTCATGCTGGCATGATCAGAATAAGAGACATAACTTCAAGCGACAACAGGGTGGAGTGGTCTATTGGTAAGGACGGGGTTGTCAACCTTGTGTTTTCTGTTCAGCTGCCGCATAGTGATATCACTTCTTCCAACCTGGTACCAATGGGCGGCTCAGTCTCTGGCAAGACGGGATCTGTGGTCATGCGGGGCTGGTTTTTGTTCATGTTCTTCTGGGTCCTATTTATTTCCATCAGTTTCAAAATCGGGAGCTATATATATGCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Helitron_like_N Herpes_Helicase PIF1 REPA_OB_2

Protein Analysis

595

Amino Acids

66.43

Weight (kDa)

4.61

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 11 - 136 1.4e-47 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000050)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_2g06412 FvH4_2g08146 FvH4_2g10321 FvH4_2g13511 FvH4_2g20780 FvH4_2g20781 FvH4_2g22171 FvH4_2g25221 FvH4_3g03500 FvH4_3g07130 FvH4_3g10140 FvH4_3g10141 FvH4_3g24350 FvH4_4g08242 FvH4_4g13410 FvH4_4g18140 FvH4_4g22470 FvH4_5g19920 FvH4_5g21501 FvH4_5g26612 FvH4_5g29621 FvH4_5g31990 FvH4_6g00280 FvH4_6g00280 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g08820 FvH4_6g29600 FvH4_6g30401 FvH4_6g33630 FvH4_7g08310 FvH4_7g08311 FvH4_7g10871 FvH4_7g18890 FvH4_7g18891
malus_domestica MD01G1202900.v1.1 MD02G1249900.v1.1 MD03G1022200.v1.1 MD03G1164900.v1.1 MD04G1002000.v1.1 MD05G1167800.v1.1 MD05G1167900.v1.1 MD05G1202200.v1.1 MD05G1215800.v1.1 MD05G1328800.v1.1 MD07G1020000.v1.1 MD07G1020100.v1.1 MD07G1084700.v1.1 MD08G1007900.v1.1 MD08G1229500.v1.1 MD09G1016000.v1.1 MD09G1184200.v1.1 MD10G1198800.v1.1 MD10G1198900.v1.1 MD11G1203900.v1.1 MD12G1081100.v1.1 MD12G1101700.v1.1 MD13G1126700.v1.1 MD13G1158300.v1.1 MD13G1161500.v1.1 MD14G1083800.v1.1 MD14G1125700.v1.1 MD14G1159700.v1.1 MD14G1159800.v1.1 MD17G1252600.v1.1 MD17G1261900.v1.1 MD17G1262000.v1.1
pyrus_communis pycom01g12270 pycom02g14690 pycom02g17560 pycom02g26880 pycom02g26890 pycom03g13090 pycom03g19990 pycom03g20000 pycom04g00130 pycom04g05050 pycom04g10460 pycom05g27190 pycom07g05970 pycom08g02820 pycom08g11570 pycom09g07420 pycom09g07430 pycom09g08580 pycom10g06760 pycom10g11210 pycom10g11220 pycom11g14380 pycom11g21990 pycom12419g00230 pycom12494g00020 pycom14g06150 pycom15g09750 pycom15g20130 pycom15g27020 pycom15g27230 pycom16g17550 pycom16g18200 pycom5153684g00260 pycom520g00040
rosa_chinensis RchiOBHm_Chr1g0320331 RchiOBHm_Chr1g0329871 RchiOBHm_Chr1g0339991 RchiOBHm_Chr1g0371171 RchiOBHm_Chr1g0378741 RchiOBHm_Chr1g0378751 RchiOBHm_Chr1g0379271 RchiOBHm_Chr2g0122381 RchiOBHm_Chr2g0133251 RchiOBHm_Chr2g0133271 RchiOBHm_Chr2g0133331 RchiOBHm_Chr2g0135141 RchiOBHm_Chr2g0141711 RchiOBHm_Chr2g0145531 RchiOBHm_Chr2g0149141 RchiOBHm_Chr2g0149151 RchiOBHm_Chr2g0150581 RchiOBHm_Chr2g0159361 RchiOBHm_Chr2g0162161 RchiOBHm_Chr2g0174211 RchiOBHm_Chr3g0484061 RchiOBHm_Chr3g0491361 RchiOBHm_Chr4g0413141 RchiOBHm_Chr4g0444601 RchiOBHm_Chr5g0004901 RchiOBHm_Chr5g0004911 RchiOBHm_Chr5g0010461 RchiOBHm_Chr5g0027941 RchiOBHm_Chr5g0028311 RchiOBHm_Chr5g0030141 RchiOBHm_Chr5g0035621 RchiOBHm_Chr5g0050871 RchiOBHm_Chr5g0053321 RchiOBHm_Chr5g0060091 RchiOBHm_Chr5g0063301 RchiOBHm_Chr5g0066571 RchiOBHm_Chr5g0067561 RchiOBHm_Chr6g0262741 RchiOBHm_Chr7g0184001 RchiOBHm_Chr7g0206181 RchiOBHm_Chr7g0212031 RchiOBHm_Chr7g0214521 RchiOBHm_Chr7g0230521 RchiOBHm_Chr7g0230621
rosa_laevigata RLG00000014424 RLG00000022069 RLG00000036206
rosa_multiflora Rmu_sc0000121.1_g000006 Rmu_sc0000271.1_g000001 Rmu_sc0000293.1_g000010 Rmu_sc0000309.1_g000052 Rmu_sc0000556.1_g000045 Rmu_sc0000837.1_g000079 Rmu_sc0001069.1_g000026 Rmu_sc0001198.1_g000001 Rmu_sc0001353.1_g000031 Rmu_sc0001461.1_g000107 Rmu_sc0002011.1_g000038 Rmu_sc0003270.1_g000034 Rmu_sc0003874.1_g000017 Rmu_sc0004729.1_g000006 Rmu_sc0007116.1_g000001 Rmu_sc0009087.1_g000019 Rmu_sc0009877.1_g000004 Rmu_sc0015060.1_g000008 Rmu_sc0016097.1_g000001
rosa_roxburghii Rroxscaffold_1G00024140 Rroxscaffold_1G00027240 Rroxscaffold_1G00030710 Rroxscaffold_1G00035010 Rroxscaffold_1G00050490 Rroxscaffold_1G00069340 Rroxscaffold_1G00072870 Rroxscaffold_2G00078360 Rroxscaffold_2G00098490 Rroxscaffold_2G00106110 Rroxscaffold_2G00109450 Rroxscaffold_2G00125260 Rroxscaffold_2G00154260 Rroxscaffold_3G00221290 Rroxscaffold_3G00221300 Rroxscaffold_3G00232770 Rroxscaffold_3G00249670 Rroxscaffold_3G00255640 Rroxscaffold_3G00255820 Rroxscaffold_3G00255870 Rroxscaffold_3G00258160 Rroxscaffold_3G00258170 Rroxscaffold_3G00265290 Rroxscaffold_4G00320550 Rroxscaffold_4G00322930 Rroxscaffold_4G00331930 Rroxscaffold_4G00332460 Rroxscaffold_5G00337730 Rroxscaffold_5G00364590 Rroxscaffold_5G00365660 Rroxscaffold_5G00373900 Rroxscaffold_6G00395750 Rroxscaffold_6G00397090 Rroxscaffold_6G00407660 Rroxscaffold_6G00421590 Rroxscaffold_7G00156820 Rroxscaffold_7G00174040 Rroxscaffold_7G00175610 Rroxscaffold_7G00182780 Rroxscaffold_7G00210390 Rroxscaffold_7G00210490 Rroxscaffold_7G00214770 Rroxscaffold_7G00215580 Rroxscaffold_7G00215690
rosa_rugosa Rorug01G0099600 Rorug01G0127300 Rorug02G0145300 Rorug02G0145400 Rorug02G0145500.1 Rorug02G0145600 Rorug02G0145700 Rorug02G0145700 Rorug02G0278400 Rorug02G0444700 Rorug02G0542800 Rorug02G0547200 Rorug04G0015900 Rorug04G0082400 Rorug04G0297900 Rorug05G0078200 Rorug05G0207900 Rorug05G0277000 Rorug05G0528600 Rorug05G0528700 Rorug05G0528800 Rorug06G0001000 Rorug06G0246500 Rorug06G0246600 Rorug06G0246700 Rorug07G0189200 Rorug07G0189300 Rorug07G0189400 Rorug07G0189500 Rorug07G0212200 Rorug07G0212200 Rorug07G0221500 Rorug07G0248800
rosa_samantha Rh1AG011600 Rh1AG052100 Rh1AG163900 Rh1BG006600 Rh1BG025100 Rh1BG025200 Rh1BG032500 Rh1BG374400 Rh1BG374500 Rh1CG153000 Rh1DG148800 Rh1DG160400 Rh1DG181900 Rh1DG405100 Rh1DG445600 Rh2AG005600 Rh2AG137100 Rh2AG150500 Rh2AG150600 Rh2AG243200 Rh2AG264300 Rh2AG349900 Rh2AG387700 Rh2AG485100 Rh2CG115600 Rh2CG269600 Rh2CG294000 Rh2CG294100 Rh2CG317700 Rh2CG375000 Rh2CG471200 Rh2CG611400 Rh2DG070000 Rh2DG073900 Rh2DG234600 Rh3AG258200 Rh3CG251500 Rh3CG283900 Rh3CG331200 Rh3CG371500 Rh3DG077300 Rh3DG185900 Rh3DG332700 Rh3DG360400 Rh4AG105100 Rh4BG191100 Rh4DG139800 Rh4DG186000 Rh4DG187900 Rh5AG302000 Rh5AG431600 Rh5CG090000 Rh5CG214700 Rh5CG299100 Rh5CG561200 Rh5DG205600 Rh5DG244500 Rh5DG257300 Rh5DG257400 Rh5DG325900 Rh5DG335500 Rh5DG359300 Rh5DG364600 Rh5DG388600 Rh6AG013900 Rh6BG045400 Rh6CG031300 Rh6CG069100 Rh6CG157500 Rh6DG029800 Rh6DG066100 Rh6DG260800 Rh6DG320100 Rh7AG007600 Rh7BG046100 Rh7BG053900 Rh7BG204900 Rh7BG322600 Rh7BG421200 Rh7DG063200 Rh7DG233200 Rh7DG287000 Rh7DG289600 Rh7DG323500 Rh7DG323800 Rh7DG323900
rosa_wichuraiana Rw0G001160 Rw0G003580 Rw0G003590 Rw0G005910 Rw0G006230 Rw0G010240 Rw0G011660 Rw0G015630 Rw0G021440 Rw0G023690 Rw1G000060 Rw1G006170 Rw1G007940 Rw1G008630 Rw1G009460 Rw1G013800 Rw1G015100 Rw1G015710 Rw1G016040 Rw1G024780 Rw2G004590 Rw2G026770 Rw2G030550 Rw2G036430 Rw3G011440 Rw3G020600 Rw3G020710 Rw3G025390 Rw3G026320 Rw4G003380 Rw4G016140 Rw4G018350 Rw4G027460 Rw5G004690 Rw5G011140 Rw5G011820 Rw5G016560 Rw5G023230 Rw5G027290 Rw5G028520 Rw5G040540 Rw5G046050 Rw6G013650 Rw6G018020 Rw6G019800 Rw6G023450 Rw7G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1598
Acc36I ACCTGC 1 cut(s) 1444
Acc65I GGTACC 2 cut(s) 485, 1657
AccB1I GGYRCC 3 cut(s) 371, 485, 1657
AccB7I CCANNNNNTGG 1 cut(s) 421
AccI GTMKAC 2 cut(s) 614, 1432
AclWI GGATC 4 cut(s) 300, 1028, 1387, 1699
AcoI YGGCCR 1 cut(s) 355
AcsI RAATTY 4 cut(s) 235, 1129, 1373, 1492
AcuI CTGAAG 5 cut(s) 101, 193, 639, 1440, 1518
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 9 cut(s) 74, 203, 272, 401, 487, 839, 1160, 1199, 1659
AfiI CCNNNNNNNGG 7 cut(s) 267, 421, 500, 501, 704, 1067, 1667
AgsI TTSAA 9 cut(s) 721, 776, 890, 931, 986, 1104, 1492, 1557, 1759
AjiI CACGTC 1 cut(s) 1369
AjnI CCWGG 6 cut(s) 163, 314, 374, 414, 565, 1653
AluBI AGCT 5 cut(s) 178, 431, 631, 1624, 1770
AluI AGCT 5 cut(s) 178, 431, 631, 1624, 1770
Alw21I GWGCWC 3 cut(s) 462, 520, 633
Alw26I GTCTC 4 cut(s) 731, 944, 1539, 1681
AlwI GGATC 4 cut(s) 300, 1028, 1387, 1699
AlwNI CAGNNNCTG 1 cut(s) 1679
Ama87I CYCGRG 1 cut(s) 32
AoxI GGCC 4 cut(s) 4, 355, 491, 608
ApaI GGGCCC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 782, 1624
ApoI RAATTY 4 cut(s) 235, 1129, 1373, 1492
ArsI GACNNNNNNTTYG 2 cut(s) 1358, 1390
Asp700I GAANNNNTTC 2 cut(s) 839, 926
Asp718I GGTACC 2 cut(s) 485, 1657
AspLEI GCGC 2 cut(s) 87, 803
AspS9I GGNCC 9 cut(s) 4, 157, 378, 491, 492, 563, 608, 707, 1735
AsuC2I CCSGG 3 cut(s) 33, 34, 490
AsuHPI GGTGA 3 cut(s) 581, 1082, 1292
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 5 cut(s) 157, 378, 563, 707, 1735
BaeGI GKGCMC 1 cut(s) 495
BanI GGYRCC 3 cut(s) 371, 485, 1657
BanII GRGCYC 3 cut(s) 364, 495, 633
BbsI GAAGAC 5 cut(s) 6, 206, 284, 337, 341
Bbv12I GWGCWC 3 cut(s) 462, 520, 633
BbvI GCAGC 2 cut(s) 769, 1611
BccI CCATC 6 cut(s) 128, 632, 638, 859, 1379, 1757
BceAI ACGGC 1 cut(s) 595
BciT130I CCWGG 6 cut(s) 165, 316, 376, 416, 567, 1655
BclI TGATCA 2 cut(s) 673, 1533
BcnI CCSGG 3 cut(s) 33, 34, 490
BcoDI GTCTC 4 cut(s) 731, 944, 1539, 1681
BfaI CTAG 1 cut(s) 1248
BfmI CTRYAG 2 cut(s) 463, 792
BfoI RGCGCY 1 cut(s) 804
BfuAI ACCTGC 1 cut(s) 1444
BisI GCNGC 4 cut(s) 783, 1625, 1628, 1669
BlpI GCTNAGC 1 cut(s) 632
BlsI GCNGC 4 cut(s) 784, 1626, 1629, 1670
BmcAI AGTACT 1 cut(s) 839
Bme1390I CCNGG 9 cut(s) 33, 34, 165, 316, 376, 416, 490, 567, 1655
Bme18I GGWCC 5 cut(s) 157, 378, 563, 707, 1735
BmeT110I CYCGRG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 1369
BmgT120I GGNCC 9 cut(s) 4, 157, 378, 491, 492, 563, 608, 707, 1735
BmiI GGNNCC 7 cut(s) 38, 158, 373, 487, 493, 1659, 1736
BmrFI CCNGG 9 cut(s) 33, 34, 165, 316, 376, 416, 490, 567, 1655
BmrI ACTGGG 1 cut(s) 294
BmsI GCATC 8 cut(s) 439, 769, 1165, 1321, 1330, 1352, 1465, 1514
BmuI ACTGGG 1 cut(s) 294
BoxI GACNNNNGTC 1 cut(s) 341
BpiI GAAGAC 5 cut(s) 6, 206, 284, 337, 341
Bpu1102I GCTNAGC 1 cut(s) 632
BpuEI CTTGAG 1 cut(s) 1495
BpuMI CCSGG 3 cut(s) 33, 34, 490
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 5 cut(s) 32, 152, 163, 164, 315
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 7 cut(s) 267, 421, 500, 501, 704, 1067, 1667
Bse118I RCCGGY 1 cut(s) 1136
Bse1I ACTGG 3 cut(s) 278, 289, 1118
BseBI CCWGG 6 cut(s) 165, 316, 376, 416, 567, 1655
BseDI CCNNGG 5 cut(s) 32, 152, 163, 164, 315
BseGI GGATG 6 cut(s) 333, 547, 649, 1018, 1186, 1505
BseLI CCNNNNNNNGG 7 cut(s) 267, 421, 500, 501, 704, 1067, 1667
BseMII CTCAG 4 cut(s) 646, 1208, 1301, 1686
BseNI ACTGG 3 cut(s) 278, 289, 1118
BseRI GAGGAG 2 cut(s) 545, 1431
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 2 cut(s) 769, 1611
BseYI CCCAGC 2 cut(s) 803, 1225
BshFI GGCC 4 cut(s) 6, 357, 493, 610
BshNI GGYRCC 3 cut(s) 371, 485, 1657
BsiHKAI GWGCWC 3 cut(s) 462, 520, 633
BsiHKCI CYCGRG 1 cut(s) 32
BsiSI CCGG 5 cut(s) 33, 96, 489, 1137, 1364
BslFI GGGAC 2 cut(s) 170, 203
BslI CCNNNNNNNGG 7 cut(s) 267, 421, 500, 501, 704, 1067, 1667
BsmAI GTCTC 4 cut(s) 731, 944, 1539, 1681
BsmFI GGGAC 2 cut(s) 170, 203
BsmI GAATGC 1 cut(s) 1227
BsnI GGCC 4 cut(s) 6, 357, 493, 610
BsoBI CYCGRG 1 cut(s) 32
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 5 cut(s) 364, 462, 495, 520, 633
Bsp143I GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
Bsp1720I GCTNAGC 1 cut(s) 632
BspANI GGCC 4 cut(s) 6, 357, 493, 610
BspCNI CTCAG 4 cut(s) 645, 1207, 1300, 1685
BspLI GGNNCC 7 cut(s) 38, 158, 373, 487, 493, 1659, 1736
BspMAI CTGCAG 2 cut(s) 467, 796
BspMI ACCTGC 1 cut(s) 1444
BspPI GGATC 4 cut(s) 300, 1028, 1387, 1699
BspQI GCTCTTC 1 cut(s) 822
BspT107I GGYRCC 3 cut(s) 371, 485, 1657
BsrFI RCCGGY 1 cut(s) 1136
BsrI ACTGG 3 cut(s) 278, 289, 1118
BssAI RCCGGY 1 cut(s) 1136
BssECI CCNNGG 5 cut(s) 32, 152, 163, 164, 315
BssMI GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
BssNAI GTATAC 2 cut(s) 615, 1433
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 152
Bst1107I GTATAC 2 cut(s) 615, 1433
Bst2UI CCWGG 6 cut(s) 165, 316, 376, 416, 567, 1655
Bst4CI ACNGT 2 cut(s) 353, 1243
Bst6I CTCTTC 3 cut(s) 473, 567, 822
BstACI GRCGYC 1 cut(s) 117
BstAPI GCANNNNNTGC 2 cut(s) 791, 1331
BstC8I GCNNGC 3 cut(s) 27, 1138, 1528
BstDEI CTNAG 5 cut(s) 632, 1084, 1194, 1287, 1672
BstF5I GGATG 6 cut(s) 333, 547, 649, 1018, 1186, 1505
BstH2I RGCGCY 1 cut(s) 804
BstHHI GCGC 2 cut(s) 87, 803
BstKTI GATC 8 cut(s) 147, 295, 676, 1036, 1099, 1382, 1536, 1694
BstMAI GTCTC 4 cut(s) 731, 944, 1539, 1681
BstMBI GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
BstMWI GCNNNNNNNGC 6 cut(s) 303, 791, 800, 1331, 1340, 1445
BstNI CCWGG 6 cut(s) 165, 316, 376, 416, 567, 1655
BstPAI GACNNNNGTC 1 cut(s) 341
BstSCI CCNGG 9 cut(s) 31, 32, 163, 314, 374, 414, 488, 565, 1653
BstSFI CTRYAG 2 cut(s) 463, 792
BstSLI GKGCMC 1 cut(s) 495
BstV1I GCAGC 2 cut(s) 769, 1611
BstV2I GAAGAC 5 cut(s) 6, 206, 284, 337, 341
BstX2I RGATCY 1 cut(s) 1691
BstXI CCANNNNNNTGG 2 cut(s) 322, 482
BstYI RGATCY 1 cut(s) 1691
BstZ17I GTATAC 2 cut(s) 615, 1433
BsuRI GGCC 4 cut(s) 6, 357, 493, 610
BtgZI GCGATG 1 cut(s) 651
BtrI CACGTC 1 cut(s) 1369
BtsCI GGATG 6 cut(s) 333, 547, 649, 1018, 1186, 1505
BtsIMutI CAGTG 1 cut(s) 358
BveI ACCTGC 1 cut(s) 1444
Cac8I GCNNGC 3 cut(s) 27, 1138, 1528
CaiI CAGNNNCTG 1 cut(s) 1679
CfoI GCGC 2 cut(s) 87, 803
Cfr10I RCCGGY 1 cut(s) 1136
Cfr13I GGNCC 9 cut(s) 4, 157, 378, 491, 492, 563, 608, 707, 1735
Cfr9I CCCGGG 1 cut(s) 32
CseI GACGC 1 cut(s) 125
CsiI ACCWGGT 2 cut(s) 565, 1653
Csp6I GTAC 9 cut(s) 73, 202, 271, 400, 486, 838, 1159, 1198, 1658
CviAII CATG 7 cut(s) 395, 731, 1328, 1523, 1531, 1702, 1723
CviQI GTAC 9 cut(s) 73, 202, 271, 400, 486, 838, 1159, 1198, 1658
DdeI CTNAG 5 cut(s) 632, 1084, 1194, 1287, 1672
DpnI GATC 8 cut(s) 146, 294, 675, 1035, 1098, 1381, 1535, 1693
DpnII GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
DraI TTTAAA 1 cut(s) 1044
DrdI GACNNNNNNGTC 1 cut(s) 1598
DseDI GACNNNNNNGTC 1 cut(s) 1598
EaeI YGGCCR 1 cut(s) 355
Eam1104I CTCTTC 3 cut(s) 473, 567, 822
EarI CTCTTC 3 cut(s) 473, 567, 822
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 152
Eco24I GRGCYC 3 cut(s) 364, 495, 633
Eco32I GATATC 2 cut(s) 136, 1639
Eco47I GGWCC 5 cut(s) 157, 378, 563, 707, 1735
Eco53kI GAGCTC 1 cut(s) 631
Eco57I CTGAAG 5 cut(s) 101, 193, 639, 1440, 1518
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 631
EcoO109I RGGNCCY 2 cut(s) 157, 1735
EcoRI GAATTC 2 cut(s) 235, 1492
EcoRII CCWGG 6 cut(s) 163, 314, 374, 414, 565, 1653
EcoRV GATATC 2 cut(s) 136, 1639
EcoT14I CCWWGG 1 cut(s) 152
EcoT22I ATGCAT 3 cut(s) 396, 454, 1345
EcoT38I GRGCYC 3 cut(s) 364, 495, 633
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 7 cut(s) 398, 734, 1331, 1526, 1534, 1705, 1726
FaqI GGGAC 2 cut(s) 170, 203
FatI CATG 7 cut(s) 394, 730, 1327, 1522, 1530, 1701, 1722
FauI CCCGC 2 cut(s) 1068, 1698
FauNDI CATATG 1 cut(s) 454
FbaI TGATCA 2 cut(s) 673, 1533
FblI GTMKAC 2 cut(s) 614, 1432
Fnu4HI GCNGC 4 cut(s) 783, 1625, 1628, 1669
FokI GGATG 6 cut(s) 340, 554, 656, 1025, 1193, 1492
FriOI GRGCYC 3 cut(s) 364, 495, 633
Fsp4HI GCNGC 4 cut(s) 783, 1625, 1628, 1669
FspBI CTAG 1 cut(s) 1248
GlaI GCGC 2 cut(s) 86, 802
GluI GCNGC 4 cut(s) 783, 1625, 1628, 1669
GsaI CCCAGC 2 cut(s) 807, 1229
HaeII RGCGCY 1 cut(s) 804
HaeIII GGCC 4 cut(s) 6, 357, 493, 610
HapII CCGG 5 cut(s) 33, 96, 489, 1137, 1364
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 87, 803
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 7 cut(s) 398, 734, 1331, 1526, 1534, 1705, 1726
Hin6I GCGC 2 cut(s) 85, 801
HinP1I GCGC 2 cut(s) 85, 801
HincII GTYRAC 2 cut(s) 1018, 1603
HindII GTYRAC 2 cut(s) 1018, 1603
HinfI GANTC 5 cut(s) 42, 250, 1003, 1211, 1414
HpaII CCGG 5 cut(s) 33, 96, 489, 1137, 1364
HphI GGTGA 3 cut(s) 581, 1082, 1292
Hpy166II GTNNAC 5 cut(s) 615, 1018, 1253, 1433, 1603
Hpy188I TCNGA 8 cut(s) 41, 678, 904, 1384, 1419, 1459, 1498, 1538
Hpy188III TCNNGA 5 cut(s) 218, 475, 698, 815, 1765
Hpy8I GTNNAC 5 cut(s) 615, 1018, 1253, 1433, 1603
Hpy99I CGWCG 4 cut(s) 119, 131, 134, 244
HpyAV CCTTC 2 cut(s) 283, 1493
HpyCH4III ACNGT 2 cut(s) 353, 1243
HpyCH4IV ACGT 1 cut(s) 1368
HpyF10VI GCNNNNNNNGC 6 cut(s) 303, 791, 800, 1331, 1340, 1445
HpyF3I CTNAG 5 cut(s) 632, 1084, 1194, 1287, 1672
HpySE526I ACGT 1 cut(s) 1368
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 7 cut(s) 398, 734, 1331, 1526, 1534, 1705, 1726
HspAI GCGC 2 cut(s) 85, 801
KpnI GGTACC 2 cut(s) 489, 1661
KroI GCCGGC 1 cut(s) 1136
KroNI GCCGGC 1 cut(s) 1138
Ksp22I TGATCA 2 cut(s) 673, 1533
Kzo9I GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
LguI GCTCTTC 1 cut(s) 822
LmnI GCTCC 5 cut(s) 34, 505, 523, 1444, 1767
Lsp1109I GCAGC 2 cut(s) 769, 1611
LweI GCATC 8 cut(s) 439, 769, 1165, 1321, 1330, 1352, 1465, 1514
MabI ACCWGGT 2 cut(s) 565, 1653
MaeI CTAG 1 cut(s) 1248
MaeII ACGT 1 cut(s) 1368
MaeIII GTNAC 1 cut(s) 1070
MalI GATC 8 cut(s) 146, 294, 675, 1035, 1098, 1381, 1535, 1693
MboI GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
MfeI CAATTG 2 cut(s) 382, 663
MflI RGATCY 1 cut(s) 1691
MhlI GDGCHC 5 cut(s) 364, 462, 495, 520, 633
MlyI GAGTC 2 cut(s) 1220, 1423
MmeI TCCRAC 1 cut(s) 1674
Mph1103I ATGCAT 3 cut(s) 396, 454, 1345
MroNI GCCGGC 1 cut(s) 1136
MroXI GAANNNNTTC 2 cut(s) 839, 926
MseI TTAA 2 cut(s) 896, 1043
MspA1I CMGCKG 2 cut(s) 603, 1624
MspI CCGG 5 cut(s) 33, 96, 489, 1137, 1364
MspR9I CCNGG 9 cut(s) 33, 34, 165, 316, 376, 416, 490, 567, 1655
MunI CAATTG 2 cut(s) 382, 663
Mva1269I GAATGC 1 cut(s) 1227
MvaI CCWGG 6 cut(s) 165, 316, 376, 416, 567, 1655
MwoI GCNNNNNNNGC 6 cut(s) 303, 791, 800, 1331, 1340, 1445
NaeI GCCGGC 1 cut(s) 1138
NciI CCSGG 3 cut(s) 33, 34, 490
NdeI CATATG 1 cut(s) 454
NdeII GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
NgoMIV GCCGGC 1 cut(s) 1136
NlaIII CATG 7 cut(s) 398, 734, 1331, 1526, 1534, 1705, 1726
NlaIV GGNNCC 7 cut(s) 38, 158, 373, 487, 493, 1659, 1736
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 1070
NsiI ATGCAT 3 cut(s) 396, 454, 1345
PasI CCCWGGG 1 cut(s) 164
PciSI GCTCTTC 1 cut(s) 822
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 245
PctI GAATGC 1 cut(s) 1227
PdiI GCCGGC 1 cut(s) 1138
PdmI GAANNNNTTC 2 cut(s) 839, 926
PfeI GAWTC 3 cut(s) 42, 250, 1003
PflMI CCANNNNNTGG 1 cut(s) 421
PkrI GCNGC 4 cut(s) 784, 1626, 1629, 1670
PleI GAGTC 2 cut(s) 1219, 1422
PpsI GAGTC 2 cut(s) 1219, 1422
PpuMI RGGWCCY 2 cut(s) 157, 1735
PshAI GACNNNNGTC 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 633
Psp5II RGGWCCY 2 cut(s) 157, 1735
Psp6I CCWGG 6 cut(s) 163, 314, 374, 414, 565, 1653
PspFI CCCAGC 2 cut(s) 803, 1225
PspGI CCWGG 6 cut(s) 163, 314, 374, 414, 565, 1653
PspN4I GGNNCC 7 cut(s) 38, 158, 373, 487, 493, 1659, 1736
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 9 cut(s) 4, 157, 378, 491, 492, 563, 608, 707, 1735
PspPPI RGGWCCY 2 cut(s) 157, 1735
PstI CTGCAG 2 cut(s) 467, 796
PstNI CAGNNNCTG 1 cut(s) 1679
PsuI RGATCY 1 cut(s) 1691
PvuII CAGCTG 1 cut(s) 1624
RsaI GTAC 9 cut(s) 74, 203, 272, 401, 487, 839, 1160, 1199, 1659
RsaNI GTAC 9 cut(s) 73, 202, 271, 400, 486, 838, 1159, 1198, 1658
SacI GAGCTC 1 cut(s) 633
SapI GCTCTTC 1 cut(s) 822
SaqAI TTAA 2 cut(s) 896, 1043
SatI GCNGC 4 cut(s) 783, 1625, 1628, 1669
Sau3AI GATC 8 cut(s) 144, 292, 673, 1033, 1096, 1379, 1533, 1691
Sau96I GGNCC 9 cut(s) 4, 157, 378, 491, 492, 563, 608, 707, 1735
ScaI AGTACT 1 cut(s) 839
SchI GAGTC 2 cut(s) 1220, 1423
ScrFI CCNGG 9 cut(s) 33, 34, 165, 316, 376, 416, 490, 567, 1655
SduI GDGCHC 5 cut(s) 364, 462, 495, 520, 633
SexAI ACCWGGT 2 cut(s) 565, 1653
SfaNI GCATC 8 cut(s) 439, 769, 1165, 1321, 1330, 1352, 1465, 1514
SfcI CTRYAG 2 cut(s) 463, 792
SinI GGWCC 5 cut(s) 157, 378, 563, 707, 1735
SmaI CCCGGG 1 cut(s) 34
SmiI ATTTAAAT 1 cut(s) 1044
SmlI CTYRAG 1 cut(s) 1510
SmoI CTYRAG 1 cut(s) 1510
SspI AATATT 2 cut(s) 682, 821
SspMI CTAG 1 cut(s) 1248
SstI GAGCTC 1 cut(s) 633
StyD4I CCNGG 9 cut(s) 31, 32, 163, 314, 374, 414, 488, 565, 1653
StyI CCWWGG 1 cut(s) 152
SwaI ATTTAAAT 1 cut(s) 1044
TaaI ACNGT 2 cut(s) 353, 1243
TaiI ACGT 1 cut(s) 1371
TaqI TCGA 8 cut(s) 114, 132, 147, 173, 242, 408, 657, 843
TatI WGTACW 2 cut(s) 837, 1197
TauI GCSGC 2 cut(s) 1630, 1671
TfiI GAWTC 3 cut(s) 42, 250, 1003
Tru1I TTAA 2 cut(s) 896, 1043
Tru9I TTAA 2 cut(s) 896, 1043
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 1 cut(s) 1070
TseI GCWGC 2 cut(s) 782, 1624
Tsp45I GTSAC 1 cut(s) 1070
TspGWI ACGGA 2 cut(s) 115, 209
TspMI CCCGGG 1 cut(s) 32
TspRI CASTG 1 cut(s) 358
Van91I CCANNNNNTGG 1 cut(s) 421
VpaK11BI GGWCC 5 cut(s) 157, 378, 563, 707, 1735
XapI RAATTY 4 cut(s) 235, 1129, 1373, 1492
XmaI CCCGGG 1 cut(s) 32
XmiI GTMKAC 2 cut(s) 614, 1432
XmnI GAANNNNTTC 2 cut(s) 839, 926
XspI CTAG 1 cut(s) 1248
ZrmI AGTACT 1 cut(s) 839
Zsp2I ATGCAT 3 cut(s) 396, 454, 1345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.