Belongs to the helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 683 |
| AccI | GTMKAC | 2 cut(s) | 687, 1023 |
| AciI | CCGC | 3 cut(s) | 625, 692, 937 |
| AcsI | RAATTY | 4 cut(s) | 635, 696, 725, 961 |
| AdeI | CACNNNGTG | 1 cut(s) | 543 |
| AfaI | GTAC | 2 cut(s) | 573, 851 |
| AjnI | CCWGG | 2 cut(s) | 453, 736 |
| AluBI | AGCT | 6 cut(s) | 59, 578, 621, 793, 909, 946 |
| AluI | AGCT | 6 cut(s) | 59, 578, 621, 793, 909, 946 |
| Alw21I | GWGCWC | 2 cut(s) | 61, 795 |
| Ama87I | CYCGRG | 1 cut(s) | 60 |
| AoxI | GGCC | 1 cut(s) | 80 |
| ApeKI | GCWGC | 3 cut(s) | 231, 870, 943 |
| ApoI | RAATTY | 4 cut(s) | 635, 696, 725, 961 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 33 |
| AspS9I | GGNCC | 1 cut(s) | 81 |
| AsuHPI | GGTGA | 2 cut(s) | 327, 752 |
| AvaI | CYCGRG | 1 cut(s) | 60 |
| BanII | GRGCYC | 2 cut(s) | 61, 795 |
| BbsI | GAAGAC | 1 cut(s) | 1044 |
| Bbv12I | GWGCWC | 2 cut(s) | 61, 795 |
| BbvI | GCAGC | 3 cut(s) | 218, 857, 955 |
| BccI | CCATC | 1 cut(s) | 104 |
| BciT130I | CCWGG | 2 cut(s) | 455, 738 |
| BclI | TGATCA | 1 cut(s) | 271 |
| BfaI | CTAG | 1 cut(s) | 888 |
| BfmI | CTRYAG | 1 cut(s) | 871 |
| BfuAI | ACCTGC | 1 cut(s) | 683 |
| BisI | GCNGC | 4 cut(s) | 232, 871, 937, 944 |
| BlsI | GCNGC | 4 cut(s) | 233, 872, 938, 945 |
| Bme1390I | CCNGG | 2 cut(s) | 455, 738 |
| BmeT110I | CYCGRG | 1 cut(s) | 60 |
| BmgT120I | GGNCC | 1 cut(s) | 81 |
| BmiI | GGNNCC | 1 cut(s) | 6 |
| BmrFI | CCNGG | 2 cut(s) | 455, 738 |
| BmsI | GCATC | 1 cut(s) | 136 |
| BpiI | GAAGAC | 1 cut(s) | 1044 |
| BpuEI | CTTGAG | 2 cut(s) | 386, 768 |
| Bsa29I | ATCGAT | 1 cut(s) | 132 |
| BsaAI | YACGTR | 1 cut(s) | 1027 |
| BsaJI | CCNNGG | 2 cut(s) | 453, 504 |
| BsaWI | WCCGGW | 1 cut(s) | 297 |
| Bse1I | ACTGG | 1 cut(s) | 182 |
| BseBI | CCWGG | 2 cut(s) | 455, 738 |
| BseCI | ATCGAT | 1 cut(s) | 132 |
| BseDI | CCNNGG | 2 cut(s) | 453, 504 |
| BseGI | GGATG | 2 cut(s) | 306, 532 |
| BseMII | CTCAG | 1 cut(s) | 529 |
| BseNI | ACTGG | 1 cut(s) | 182 |
| BseXI | GCAGC | 3 cut(s) | 218, 857, 955 |
| BshFI | GGCC | 1 cut(s) | 82 |
| BshVI | ATCGAT | 1 cut(s) | 132 |
| BsiHKAI | GWGCWC | 2 cut(s) | 61, 795 |
| BsiHKCI | CYCGRG | 1 cut(s) | 60 |
| BsiSI | CCGG | 1 cut(s) | 298 |
| BslFI | GGGAC | 1 cut(s) | 436 |
| BsmFI | GGGAC | 1 cut(s) | 436 |
| BsmI | GAATGC | 1 cut(s) | 353 |
| BsnI | GGCC | 1 cut(s) | 82 |
| BsoBI | CYCGRG | 1 cut(s) | 60 |
| Bsp1286I | GDGCHC | 2 cut(s) | 61, 795 |
| Bsp1407I | TGTACA | 2 cut(s) | 571, 849 |
| Bsp143I | GATC | 4 cut(s) | 271, 397, 600, 799 |
| BspACI | CCGC | 3 cut(s) | 625, 692, 937 |
| BspANI | GGCC | 1 cut(s) | 82 |
| BspCNI | CTCAG | 1 cut(s) | 528 |
| BspDI | ATCGAT | 1 cut(s) | 132 |
| BspHI | TCATGA | 1 cut(s) | 274 |
| BspLI | GGNNCC | 1 cut(s) | 6 |
| BspMAI | CTGCAG | 1 cut(s) | 875 |
| BspMI | ACCTGC | 1 cut(s) | 683 |
| BspQI | GCTCTTC | 1 cut(s) | 783 |
| BsrGI | TGTACA | 2 cut(s) | 571, 849 |
| BsrI | ACTGG | 1 cut(s) | 182 |
| BssECI | CCNNGG | 2 cut(s) | 453, 504 |
| BssMI | GATC | 4 cut(s) | 271, 397, 600, 799 |
| BssNAI | GTATAC | 1 cut(s) | 1024 |
| BssT1I | CCWWGG | 1 cut(s) | 504 |
| Bst1107I | GTATAC | 1 cut(s) | 1024 |
| Bst2UI | CCWGG | 2 cut(s) | 455, 738 |
| Bst4CI | ACNGT | 2 cut(s) | 817, 923 |
| Bst6I | CTCTTC | 2 cut(s) | 334, 783 |
| BstAUI | TGTACA | 2 cut(s) | 571, 849 |
| BstBAI | YACGTR | 1 cut(s) | 1027 |
| BstC8I | GCNNGC | 2 cut(s) | 623, 941 |
| BstDEI | CTNAG | 2 cut(s) | 515, 604 |
| BstF5I | GGATG | 2 cut(s) | 306, 532 |
| BstKTI | GATC | 4 cut(s) | 274, 400, 603, 802 |
| BstMBI | GATC | 4 cut(s) | 271, 397, 600, 799 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 65, 155, 884 |
| BstNI | CCWGG | 2 cut(s) | 455, 738 |
| BstNSI | RCATGY | 1 cut(s) | 835 |
| BstSCI | CCNGG | 2 cut(s) | 453, 736 |
| BstSFI | CTRYAG | 1 cut(s) | 871 |
| BstSNI | TACGTA | 1 cut(s) | 1027 |
| BstV1I | GCAGC | 3 cut(s) | 218, 857, 955 |
| BstV2I | GAAGAC | 1 cut(s) | 1044 |
| BstZ17I | GTATAC | 1 cut(s) | 1024 |
| Bsu15I | ATCGAT | 1 cut(s) | 132 |
| BsuRI | GGCC | 1 cut(s) | 82 |
| BsuTUI | ATCGAT | 1 cut(s) | 132 |
| BtgZI | GCGATG | 1 cut(s) | 159 |
| BtsCI | GGATG | 2 cut(s) | 306, 532 |
| BtsIMutI | CAGTG | 1 cut(s) | 379 |
| BveI | ACCTGC | 1 cut(s) | 683 |
| Cac8I | GCNNGC | 2 cut(s) | 623, 941 |
| CciI | TCATGA | 1 cut(s) | 274 |
| Cfr13I | GGNCC | 1 cut(s) | 81 |
| ClaI | ATCGAT | 1 cut(s) | 132 |
| Csp6I | GTAC | 2 cut(s) | 572, 850 |
| CviAII | CATG | 5 cut(s) | 155, 275, 442, 679, 832 |
| CviQI | GTAC | 2 cut(s) | 572, 850 |
| DdeI | CTNAG | 2 cut(s) | 515, 604 |
| DpnI | GATC | 4 cut(s) | 273, 399, 602, 801 |
| DpnII | GATC | 4 cut(s) | 271, 397, 600, 799 |
| DraI | TTTAAA | 1 cut(s) | 772 |
| DraIII | CACNNNGTG | 1 cut(s) | 543 |
| Eam1104I | CTCTTC | 2 cut(s) | 334, 783 |
| EarI | CTCTTC | 2 cut(s) | 334, 783 |
| Ecl136II | GAGCTC | 2 cut(s) | 59, 793 |
| Eco105I | TACGTA | 1 cut(s) | 1027 |
| Eco130I | CCWWGG | 1 cut(s) | 504 |
| Eco24I | GRGCYC | 2 cut(s) | 61, 795 |
| Eco32I | GATATC | 1 cut(s) | 103 |
| Eco53kI | GAGCTC | 2 cut(s) | 59, 793 |
| Eco88I | CYCGRG | 1 cut(s) | 60 |
| EcoICRI | GAGCTC | 2 cut(s) | 59, 793 |
| EcoRI | GAATTC | 2 cut(s) | 635, 725 |
| EcoRII | CCWGG | 2 cut(s) | 453, 736 |
| EcoRV | GATATC | 1 cut(s) | 103 |
| EcoT14I | CCWWGG | 1 cut(s) | 504 |
| EcoT22I | ATGCAT | 1 cut(s) | 355 |
| EcoT38I | GRGCYC | 2 cut(s) | 61, 795 |
| ErhI | CCWWGG | 1 cut(s) | 504 |
| FaeI | CATG | 5 cut(s) | 158, 278, 445, 682, 835 |
| FaqI | GGGAC | 1 cut(s) | 436 |
| FatI | CATG | 5 cut(s) | 154, 274, 441, 678, 831 |
| FauI | CCCGC | 1 cut(s) | 699 |
| FbaI | TGATCA | 1 cut(s) | 271 |
| FblI | GTMKAC | 2 cut(s) | 687, 1023 |
| Fnu4HI | GCNGC | 4 cut(s) | 232, 871, 937, 944 |
| FokI | GGATG | 2 cut(s) | 313, 539 |
| FriOI | GRGCYC | 2 cut(s) | 61, 795 |
| Fsp4HI | GCNGC | 4 cut(s) | 232, 871, 937, 944 |
| FspBI | CTAG | 1 cut(s) | 888 |
| GluI | GCNGC | 4 cut(s) | 232, 871, 937, 944 |
| HaeIII | GGCC | 1 cut(s) | 82 |
| HapII | CCGG | 1 cut(s) | 298 |
| Hin1II | CATG | 5 cut(s) | 158, 278, 445, 682, 835 |
| HindIII | AAGCTT | 1 cut(s) | 619 |
| HinfI | GANTC | 9 cut(s) | 170, 326, 340, 449, 485, 648, 667, 754, 891 |
| HpaII | CCGG | 1 cut(s) | 298 |
| HphI | GGTGA | 2 cut(s) | 327, 752 |
| Hpy166II | GTNNAC | 2 cut(s) | 688, 1024 |
| Hpy188I | TCNGA | 3 cut(s) | 402, 411, 823 |
| Hpy188III | TCNNGA | 6 cut(s) | 240, 275, 323, 517, 660, 785 |
| Hpy8I | GTNNAC | 2 cut(s) | 688, 1024 |
| HpyAV | CCTTC | 1 cut(s) | 200 |
| HpyCH4III | ACNGT | 2 cut(s) | 817, 923 |
| HpyCH4IV | ACGT | 1 cut(s) | 1026 |
| HpyCH4V | TGCA | 3 cut(s) | 353, 678, 873 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 65, 155, 884 |
| HpyF3I | CTNAG | 2 cut(s) | 515, 604 |
| HpySE526I | ACGT | 1 cut(s) | 1026 |
| Hsp92II | CATG | 5 cut(s) | 158, 278, 445, 682, 835 |
| Ksp22I | TGATCA | 1 cut(s) | 271 |
| Kzo9I | GATC | 4 cut(s) | 271, 397, 600, 799 |
| LguI | GCTCTTC | 1 cut(s) | 783 |
| LmnI | GCTCC | 1 cut(s) | 10 |
| Lsp1109I | GCAGC | 3 cut(s) | 218, 857, 955 |
| LweI | GCATC | 1 cut(s) | 136 |
| MaeI | CTAG | 1 cut(s) | 888 |
| MaeII | ACGT | 1 cut(s) | 1026 |
| MaeIII | GTNAC | 3 cut(s) | 187, 740, 1027 |
| MalI | GATC | 4 cut(s) | 273, 399, 602, 801 |
| MboI | GATC | 4 cut(s) | 271, 397, 600, 799 |
| MboII | GAAGA | 5 cut(s) | 59, 321, 407, 800, 1049 |
| MhlI | GDGCHC | 2 cut(s) | 61, 795 |
| MluCI | AATT | 7 cut(s) | 38, 162, 563, 635, 696, 725, 961 |
| MlyI | GAGTC | 2 cut(s) | 320, 458 |
| MmeI | TCCRAC | 1 cut(s) | 664 |
| MnlI | CCTC | 9 cut(s) | 72, 196, 271, 296, 343, 371, 459, 517, 711 |
| Mph1103I | ATGCAT | 1 cut(s) | 355 |
| MroXI | GAANNNNTTC | 1 cut(s) | 33 |
| MseI | TTAA | 5 cut(s) | 294, 531, 771, 837, 984 |
| MspI | CCGG | 1 cut(s) | 298 |
| MspR9I | CCNGG | 2 cut(s) | 455, 738 |
| Mva1269I | GAATGC | 1 cut(s) | 353 |
| MvaI | CCWGG | 2 cut(s) | 455, 738 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 65, 155, 884 |
| NdeII | GATC | 4 cut(s) | 271, 397, 600, 799 |
| NlaIII | CATG | 5 cut(s) | 158, 278, 445, 682, 835 |
| NlaIV | GGNNCC | 1 cut(s) | 6 |
| NmuCI | GTSAC | 1 cut(s) | 740 |
| NsiI | ATGCAT | 1 cut(s) | 355 |
| NspI | RCATGY | 1 cut(s) | 835 |
| PagI | TCATGA | 1 cut(s) | 274 |
| PciSI | GCTCTTC | 1 cut(s) | 783 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1032 |
| PctI | GAATGC | 1 cut(s) | 353 |
| PdmI | GAANNNNTTC | 1 cut(s) | 33 |
| PfeI | GAWTC | 7 cut(s) | 170, 340, 485, 648, 667, 754, 891 |
| PkrI | GCNGC | 4 cut(s) | 233, 872, 938, 945 |
| PleI | GAGTC | 2 cut(s) | 320, 457 |
| PpsI | GAGTC | 2 cut(s) | 320, 457 |
| Ppu21I | YACGTR | 1 cut(s) | 1027 |
| Psp124BI | GAGCTC | 2 cut(s) | 61, 795 |
| Psp6I | CCWGG | 2 cut(s) | 453, 736 |
| PspGI | CCWGG | 2 cut(s) | 453, 736 |
| PspN4I | GGNNCC | 1 cut(s) | 6 |
| PspPI | GGNCC | 1 cut(s) | 81 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 564, 596, 655, 687 |
| PstI | CTGCAG | 1 cut(s) | 875 |
| RsaI | GTAC | 2 cut(s) | 573, 851 |
| RsaNI | GTAC | 2 cut(s) | 572, 850 |
| SacI | GAGCTC | 2 cut(s) | 61, 795 |
| SapI | GCTCTTC | 1 cut(s) | 783 |
| SaqAI | TTAA | 5 cut(s) | 294, 531, 771, 837, 984 |
| SatI | GCNGC | 4 cut(s) | 232, 871, 937, 944 |
| Sau3AI | GATC | 4 cut(s) | 271, 397, 600, 799 |
| Sau96I | GGNCC | 1 cut(s) | 81 |
| SchI | GAGTC | 2 cut(s) | 320, 458 |
| ScrFI | CCNGG | 2 cut(s) | 455, 738 |
| SduI | GDGCHC | 2 cut(s) | 61, 795 |
| SfaNI | GCATC | 1 cut(s) | 136 |
| SfcI | CTRYAG | 1 cut(s) | 871 |
| SmlI | CTYRAG | 2 cut(s) | 365, 783 |
| SmoI | CTYRAG | 2 cut(s) | 365, 783 |
| SnaBI | TACGTA | 1 cut(s) | 1027 |
| Sse9I | AATT | 7 cut(s) | 38, 162, 563, 635, 696, 725, 961 |
| SsiI | CCGC | 3 cut(s) | 625, 692, 937 |
| SspMI | CTAG | 1 cut(s) | 888 |
| SstI | GAGCTC | 2 cut(s) | 61, 795 |
| StyD4I | CCNGG | 2 cut(s) | 453, 736 |
| StyI | CCWWGG | 1 cut(s) | 504 |
| TaaI | ACNGT | 2 cut(s) | 817, 923 |
| TaiI | ACGT | 1 cut(s) | 1029 |
| TaqI | TCGA | 3 cut(s) | 132, 802, 1035 |
| TasI | AATT | 7 cut(s) | 38, 162, 563, 635, 696, 725, 961 |
| TatI | WGTACW | 2 cut(s) | 571, 849 |
| TauI | GCSGC | 1 cut(s) | 939 |
| TfiI | GAWTC | 7 cut(s) | 170, 340, 485, 648, 667, 754, 891 |
| Tru1I | TTAA | 5 cut(s) | 294, 531, 771, 837, 984 |
| Tru9I | TTAA | 5 cut(s) | 294, 531, 771, 837, 984 |
| TscAI | CASTG | 1 cut(s) | 379 |
| TseFI | GTSAC | 1 cut(s) | 740 |
| TseI | GCWGC | 3 cut(s) | 231, 870, 943 |
| Tsp45I | GTSAC | 1 cut(s) | 740 |
| TspDTI | ATGAA | 6 cut(s) | 31, 107, 150, 183, 738, 984 |
| TspGWI | ACGGA | 1 cut(s) | 8 |
| TspRI | CASTG | 1 cut(s) | 379 |
| XapI | RAATTY | 4 cut(s) | 635, 696, 725, 961 |
| XceI | RCATGY | 1 cut(s) | 835 |
| XmiI | GTMKAC | 2 cut(s) | 687, 1023 |
| XmnI | GAANNNNTTC | 1 cut(s) | 33 |
| XspI | CTAG | 1 cut(s) | 888 |
| Zsp2I | ATGCAT | 1 cut(s) | 355 |