Rorug05G0078200

Belongs to the helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000050
Physical Location & Seq
Forward (+)
6770560 .. 6775146
4587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0078200.1

Sequence Viewer

Length: 2142 bp
ATGGAGGCGAAGAATAGCGGCAAACCCATACTTCAATTCGCACCGTTTCAGAGCTCCGTCGACGAGGGTTTCTGGCACAGATTGTCTTCTTTGAAGCTCAACAAGTTTGGCATCGACGACTCTCCAATTCCCATCACTGGCTTCTATGCGCCGTGCTCACATTCTCAGGTGTCAAATCATTTGACTCTTCTAGCTGAGTCTTTGCCTTCGGATTCGAATGAAGAATCGAGTGAACCAGGAGTGATTCGGGGAAATAGGAATAGATGCTCCGTTCCCGGTGTTCTTTACAACACCAATACGGTAGAAGGCTTTCGTGCACTTGACAAACAGGAGTTGATTAAAGCAGAAGCGAAAAAGATTTGGGAGGACATTCAGAATGGAAAAGCTTTGGAGGATAGTTCTGTACTTTCCAGGTTCCTCCTTATCTCTTTTGCAGACCTTAAAAAATGGAGCTTTCATTACTGGTTTGCCTTCCCTGCTCTGGTGCTTGATCCTCCAGCAACATTGCTTGATTTGAAACCAGCTTCACAATTTTTTAGTGTGAAGGAGGCAGAATCTGTCTCAGCAGTATTAAATGACTGGCGCGACTCAAGCTTAACTACAGATGTGCCGTTCTTTTTGGTTGTAATTGATCCAAATTCGCGTGCTACCATTAAGCATCTAAAGGATTGGGAAACTTGTCAAAGAGCTACTGATCACAAGTTGCTATTCGGTTTTTACGACCCATGCCATCTTCCAAAGAATCCTGGTTGGCCGCTTCGGAACTTGCTTGCACTTATTTGCTCAAGATGGGATCTCAAGTCTGTTCGCTTTCTATGCTATAGAGAGACTCGTGGTTTCGCAGATCTAGGATTGTCCCTTGTTGGTGAAGCCCAGGTTACAGTTCCCCAAGGATGGGGAGATCTTCATAATACACCCAATGCAGTGGGGTGGGAACTTAACAAAGGGAAAAAAATACCCAGGTGTATTAGCCTTGCTAAATCTATGGATCCAACTAGGTTGGCCGTATCTGCTGCAGATTTGAATTTGAAACTAATGAGATGGCGTGCTTTACCTTCTCTCAAGTTAAATACCTTGTCCTCTCTCAAGTGTCTCCTCCTTGGAGCAGGTACACTTGGATGCCAGGTTGCTCGCATGCTTATGGCTTGGGGCGTTCGGAAAATTACGTTGGTTGACAATGGCAGAGTGGCTATGTCTAATCCCTTGAGGCAATCCCTGTATACATTAGATGACTGTCTAAATGGTGGTGAATTTAAAGCAGTAGCAGCAGTTAACAGTCTTAAGAAAATATTTCCCGCTGTGGAAGCAGAGGGTGTTGTCATGGCTATACCAATGCCTGGACATCCCGTTCCTAGTCAAGAAGAGCAGAGTGTGCTTGATGATTGTAGGCGCCTACATGATTTAATTGATTCTCATGATGCAGTTTTCTTGTTAACTGATACAAGAGAAAGTCGGTGGCTTCCGTCTCTTCTTTGTGCCAATACTAACAAGATTACTATAACTGCAGCTCTTGGGTTTGATAGCTTTTTAGTTATGCGCCATGGAGCAGGTCCATGCAGCTCTAGCCATGACTCAAAATCTGAAGCTGCAAATGCTTTATCCACTGATCTGAACAGCCTTGCACTGACTGATAGAAATGGAGGGCACAGATTGGGTTGCTACTTCTGCAATGATGTGGTTGCACCTATTGATTCAACTGCCAACCGCACCTTGGACCAGCAATGCACTGTTACACGCCCAGGCCTCGCTCCTATTGCCTCAGCTCTTGCAGTTGAGCTCTTAGTTGGGATCCTACATCACCCTGATGGGATATTTGCCGAAGGTGAAATTGCAAACTCTAGTAATAATGGAAGCAGTGAGCAACCTCTTGGTATTTTACCCCATCAAATCCGGGGTTCCCTTGGACAGTTTTCGCAGATGACACTTGTGGGCCACTCCTCAGACAGTTGCACGGCTTGCTGCAGCATTGTGGTGTCTGAATATCGAAAAAGGGGAACGGAATTTATACTACAAGCAATCAACCATCCCACCTATCTGGAGGACGTGACTGGACTAACAGAGTTGATGAAAACAGCTCATGCATTTCAAATGGATTGGGATGATGGGACTGATAATGATGATGATGATTGTGTTGAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Helitron_like_N Herpes_Helicase PIF1 REPA_OB_2

Protein Analysis

713

Amino Acids

78.3

Weight (kDa)

5.75

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG7_N PF16420 11 - 329 3.3e-103 Ubiquitin-like modifier-activating enzyme ATG7 N-terminus
ThiF PF00899 346 - 602 8.9e-38 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000050)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_2g06412 FvH4_2g08146 FvH4_2g10321 FvH4_2g13511 FvH4_2g20780 FvH4_2g20781 FvH4_2g22171 FvH4_2g25221 FvH4_3g03500 FvH4_3g07130 FvH4_3g10140 FvH4_3g10141 FvH4_3g24350 FvH4_4g08242 FvH4_4g13410 FvH4_4g18140 FvH4_4g22470 FvH4_5g19920 FvH4_5g21501 FvH4_5g26612 FvH4_5g29621 FvH4_5g31990 FvH4_6g00280 FvH4_6g00280 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g08820 FvH4_6g29600 FvH4_6g30401 FvH4_6g33630 FvH4_7g08310 FvH4_7g08311 FvH4_7g10871 FvH4_7g18890 FvH4_7g18891
malus_domestica MD01G1202900.v1.1 MD02G1249900.v1.1 MD03G1022200.v1.1 MD03G1164900.v1.1 MD04G1002000.v1.1 MD05G1167800.v1.1 MD05G1167900.v1.1 MD05G1202200.v1.1 MD05G1215800.v1.1 MD05G1328800.v1.1 MD07G1020000.v1.1 MD07G1020100.v1.1 MD07G1084700.v1.1 MD08G1007900.v1.1 MD08G1229500.v1.1 MD09G1016000.v1.1 MD09G1184200.v1.1 MD10G1198800.v1.1 MD10G1198900.v1.1 MD11G1203900.v1.1 MD12G1081100.v1.1 MD12G1101700.v1.1 MD13G1126700.v1.1 MD13G1158300.v1.1 MD13G1161500.v1.1 MD14G1083800.v1.1 MD14G1125700.v1.1 MD14G1159700.v1.1 MD14G1159800.v1.1 MD17G1252600.v1.1 MD17G1261900.v1.1 MD17G1262000.v1.1
pyrus_communis pycom01g12270 pycom02g14690 pycom02g17560 pycom02g26880 pycom02g26890 pycom03g13090 pycom03g19990 pycom03g20000 pycom04g00130 pycom04g05050 pycom04g10460 pycom05g27190 pycom07g05970 pycom08g02820 pycom08g11570 pycom09g07420 pycom09g07430 pycom09g08580 pycom10g06760 pycom10g11210 pycom10g11220 pycom11g14380 pycom11g21990 pycom12419g00230 pycom12494g00020 pycom14g06150 pycom15g09750 pycom15g20130 pycom15g27020 pycom15g27230 pycom16g17550 pycom16g18200 pycom5153684g00260 pycom520g00040
rosa_chinensis RchiOBHm_Chr1g0320331 RchiOBHm_Chr1g0329871 RchiOBHm_Chr1g0339991 RchiOBHm_Chr1g0371171 RchiOBHm_Chr1g0378741 RchiOBHm_Chr1g0378751 RchiOBHm_Chr1g0379271 RchiOBHm_Chr2g0122381 RchiOBHm_Chr2g0133251 RchiOBHm_Chr2g0133271 RchiOBHm_Chr2g0133331 RchiOBHm_Chr2g0135141 RchiOBHm_Chr2g0141711 RchiOBHm_Chr2g0145531 RchiOBHm_Chr2g0149141 RchiOBHm_Chr2g0149151 RchiOBHm_Chr2g0150581 RchiOBHm_Chr2g0159361 RchiOBHm_Chr2g0162161 RchiOBHm_Chr2g0174211 RchiOBHm_Chr3g0484061 RchiOBHm_Chr3g0491361 RchiOBHm_Chr4g0413141 RchiOBHm_Chr4g0444601 RchiOBHm_Chr5g0004901 RchiOBHm_Chr5g0004911 RchiOBHm_Chr5g0010461 RchiOBHm_Chr5g0027941 RchiOBHm_Chr5g0028311 RchiOBHm_Chr5g0030141 RchiOBHm_Chr5g0035621 RchiOBHm_Chr5g0050871 RchiOBHm_Chr5g0053321 RchiOBHm_Chr5g0060091 RchiOBHm_Chr5g0063301 RchiOBHm_Chr5g0066571 RchiOBHm_Chr5g0067561 RchiOBHm_Chr6g0262741 RchiOBHm_Chr7g0184001 RchiOBHm_Chr7g0206181 RchiOBHm_Chr7g0212031 RchiOBHm_Chr7g0214521 RchiOBHm_Chr7g0230521 RchiOBHm_Chr7g0230621
rosa_laevigata RLG00000014424 RLG00000022069 RLG00000036206
rosa_multiflora Rmu_sc0000121.1_g000006 Rmu_sc0000271.1_g000001 Rmu_sc0000293.1_g000010 Rmu_sc0000309.1_g000052 Rmu_sc0000556.1_g000045 Rmu_sc0000837.1_g000079 Rmu_sc0001069.1_g000026 Rmu_sc0001198.1_g000001 Rmu_sc0001353.1_g000031 Rmu_sc0001461.1_g000107 Rmu_sc0002011.1_g000038 Rmu_sc0003270.1_g000034 Rmu_sc0003874.1_g000017 Rmu_sc0004729.1_g000006 Rmu_sc0007116.1_g000001 Rmu_sc0009087.1_g000019 Rmu_sc0009877.1_g000004 Rmu_sc0015060.1_g000008 Rmu_sc0016097.1_g000001
rosa_roxburghii Rroxscaffold_1G00024140 Rroxscaffold_1G00027240 Rroxscaffold_1G00030710 Rroxscaffold_1G00035010 Rroxscaffold_1G00050490 Rroxscaffold_1G00069340 Rroxscaffold_1G00072870 Rroxscaffold_2G00078360 Rroxscaffold_2G00098490 Rroxscaffold_2G00106110 Rroxscaffold_2G00109450 Rroxscaffold_2G00125260 Rroxscaffold_2G00154260 Rroxscaffold_3G00221290 Rroxscaffold_3G00221300 Rroxscaffold_3G00232770 Rroxscaffold_3G00249670 Rroxscaffold_3G00255640 Rroxscaffold_3G00255820 Rroxscaffold_3G00255870 Rroxscaffold_3G00258160 Rroxscaffold_3G00258170 Rroxscaffold_3G00265290 Rroxscaffold_4G00320550 Rroxscaffold_4G00322930 Rroxscaffold_4G00331930 Rroxscaffold_4G00332460 Rroxscaffold_5G00337730 Rroxscaffold_5G00364590 Rroxscaffold_5G00365660 Rroxscaffold_5G00373900 Rroxscaffold_6G00395750 Rroxscaffold_6G00397090 Rroxscaffold_6G00407660 Rroxscaffold_6G00421590 Rroxscaffold_7G00156820 Rroxscaffold_7G00174040 Rroxscaffold_7G00175610 Rroxscaffold_7G00182780 Rroxscaffold_7G00210390 Rroxscaffold_7G00210490 Rroxscaffold_7G00214770 Rroxscaffold_7G00215580 Rroxscaffold_7G00215690
rosa_rugosa Rorug01G0099600 Rorug01G0127300 Rorug02G0145300 Rorug02G0145400 Rorug02G0145500.1 Rorug02G0145600 Rorug02G0145700 Rorug02G0145700 Rorug02G0278400 Rorug02G0444700 Rorug02G0542800 Rorug02G0547200 Rorug04G0015900 Rorug04G0082400 Rorug04G0297900 Rorug05G0078200 Rorug05G0207900 Rorug05G0277000 Rorug05G0528600 Rorug05G0528700 Rorug05G0528800 Rorug06G0001000 Rorug06G0246500 Rorug06G0246600 Rorug06G0246700 Rorug07G0189200 Rorug07G0189300 Rorug07G0189400 Rorug07G0189500 Rorug07G0212200 Rorug07G0212200 Rorug07G0221500 Rorug07G0248800
rosa_samantha Rh1AG011600 Rh1AG052100 Rh1AG163900 Rh1BG006600 Rh1BG025100 Rh1BG025200 Rh1BG032500 Rh1BG374400 Rh1BG374500 Rh1CG153000 Rh1DG148800 Rh1DG160400 Rh1DG181900 Rh1DG405100 Rh1DG445600 Rh2AG005600 Rh2AG137100 Rh2AG150500 Rh2AG150600 Rh2AG243200 Rh2AG264300 Rh2AG349900 Rh2AG387700 Rh2AG485100 Rh2CG115600 Rh2CG269600 Rh2CG294000 Rh2CG294100 Rh2CG317700 Rh2CG375000 Rh2CG471200 Rh2CG611400 Rh2DG070000 Rh2DG073900 Rh2DG234600 Rh3AG258200 Rh3CG251500 Rh3CG283900 Rh3CG331200 Rh3CG371500 Rh3DG077300 Rh3DG185900 Rh3DG332700 Rh3DG360400 Rh4AG105100 Rh4BG191100 Rh4DG139800 Rh4DG186000 Rh4DG187900 Rh5AG302000 Rh5AG431600 Rh5CG090000 Rh5CG214700 Rh5CG299100 Rh5CG561200 Rh5DG205600 Rh5DG244500 Rh5DG257300 Rh5DG257400 Rh5DG325900 Rh5DG335500 Rh5DG359300 Rh5DG364600 Rh5DG388600 Rh6AG013900 Rh6BG045400 Rh6CG031300 Rh6CG069100 Rh6CG157500 Rh6DG029800 Rh6DG066100 Rh6DG260800 Rh6DG320100 Rh7AG007600 Rh7BG046100 Rh7BG053900 Rh7BG204900 Rh7BG322600 Rh7BG421200 Rh7DG063200 Rh7DG233200 Rh7DG287000 Rh7DG289600 Rh7DG323500 Rh7DG323800 Rh7DG323900
rosa_wichuraiana Rw0G001160 Rw0G003580 Rw0G003590 Rw0G005910 Rw0G006230 Rw0G010240 Rw0G011660 Rw0G015630 Rw0G021440 Rw0G023690 Rw1G000060 Rw1G006170 Rw1G007940 Rw1G008630 Rw1G009460 Rw1G013800 Rw1G015100 Rw1G015710 Rw1G016040 Rw1G024780 Rw2G004590 Rw2G026770 Rw2G030550 Rw2G036430 Rw3G011440 Rw3G020600 Rw3G020710 Rw3G025390 Rw3G026320 Rw4G003380 Rw4G016140 Rw4G018350 Rw4G027460 Rw5G004690 Rw5G011140 Rw5G011820 Rw5G016560 Rw5G023230 Rw5G027290 Rw5G028520 Rw5G040540 Rw5G046050 Rw6G013650 Rw6G018020 Rw6G019800 Rw6G023450 Rw7G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1097, 1538
AccB1I GGYRCC 1 cut(s) 1389
AccB7I CCANNNNNTGG 1 cut(s) 1337
AccI GTMKAC 2 cut(s) 60, 1220
AccII CGCG 2 cut(s) 585, 643
AciI CCGC 4 cut(s) 18, 755, 1296, 1705
AclWI GGATC 7 cut(s) 485, 626, 801, 983, 996, 1783, 1796
AcoI YGGCCR 2 cut(s) 752, 1002
AcsI RAATTY 5 cut(s) 637, 1024, 1250, 2000, 2136
AcuI CTGAAG 1 cut(s) 1602
AcyI GRCGYC 1 cut(s) 1390
AfaI GTAC 2 cut(s) 405, 1111
AfiI CCNNNNNNNGG 6 cut(s) 137, 481, 894, 895, 1337, 1711
AflII CTTAAG 1 cut(s) 1280
AgsI TTSAA 8 cut(s) 35, 94, 517, 1024, 1030, 1695, 2087, 2135
AjiI CACGTC 1 cut(s) 2044
AjnI CCWGG 8 cut(s) 235, 410, 745, 873, 959, 1122, 1336, 1738
AjuI GAANNNNNNNTTGG 2 cut(s) 1151, 1183
AloI GAACNNNNNNTCC 2 cut(s) 1332, 1364
Alw21I GWGCWC 4 cut(s) 56, 158, 319, 1779
Alw26I GTCTC 4 cut(s) 565, 821, 1097, 1470
Alw44I GTGCAC 1 cut(s) 315
AlwI GGATC 7 cut(s) 485, 626, 801, 983, 996, 1783, 1796
AlwNI CAGNNNCTG 1 cut(s) 557
AoxI GGCC 4 cut(s) 752, 1002, 1741, 1930
ApaLI GTGCAC 1 cut(s) 315
ApeKI GCWGC 7 cut(s) 1013, 1265, 1505, 1557, 1586, 1959, 1962
ApoI RAATTY 5 cut(s) 637, 1024, 1250, 2000, 2136
Asp700I GAANNNNTTC 1 cut(s) 309
AspLEI GCGC 4 cut(s) 151, 585, 1392, 1539
AspS9I GGNCC 3 cut(s) 1550, 1714, 1930
AsuC2I CCSGG 2 cut(s) 276, 1892
AsuHPI GGTGA 4 cut(s) 878, 1259, 1790, 1835
AsuII TTCGAA 1 cut(s) 215
AvaII GGWCC 2 cut(s) 1550, 1714
BaeGI GKGCMC 2 cut(s) 319, 1647
BamHI GGATCC 2 cut(s) 988, 1788
BanI GGYRCC 1 cut(s) 1389
BanII GRGCYC 2 cut(s) 56, 1779
BauI CACGAG 1 cut(s) 831
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 4 cut(s) 56, 158, 319, 1779
BbvCI CCTCAGC 1 cut(s) 1759
BbvI GCAGC 7 cut(s) 1000, 1277, 1517, 1569, 1573, 1946, 1974
BccI CCATC 9 cut(s) 140, 738, 783, 888, 1035, 1799, 1890, 2031, 2096
BceAI ACGGC 4 cut(s) 136, 595, 989, 1968
BciT130I CCWGG 8 cut(s) 237, 412, 747, 875, 961, 1124, 1338, 1740
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 2 cut(s) 276, 1892
BcoDI GTCTC 4 cut(s) 565, 821, 1097, 1470
BfaI CTAG 6 cut(s) 191, 848, 996, 1353, 1563, 1839
BfmI CTRYAG 5 cut(s) 600, 820, 1014, 1503, 1960
BfoI RGCGCY 1 cut(s) 1393
BfrI CTTAAG 1 cut(s) 1280
BfuAI ACCTGC 2 cut(s) 1097, 1538
BglII AGATCT 2 cut(s) 844, 901
BisI GCNGC 9 cut(s) 19, 755, 1014, 1266, 1506, 1558, 1587, 1960, 1963
BlsI GCNGC 9 cut(s) 20, 756, 1015, 1267, 1507, 1559, 1588, 1961, 1964
Bme18I GGWCC 2 cut(s) 1550, 1714
BmgBI CACGTC 1 cut(s) 2044
BmgT120I GGNCC 3 cut(s) 1550, 1714, 1930
BmiI GGNNCC 5 cut(s) 416, 990, 1391, 1790, 1897
BmsI GCATC 5 cut(s) 120, 254, 667, 1109, 1408
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 2 cut(s) 480, 2057
Bpu10I CCTNAGC 1 cut(s) 1759
Bpu14I TTCGAA 1 cut(s) 215
BpuEI CTTGAG 6 cut(s) 574, 769, 782, 1046, 1070, 1225
BpuMI CCSGG 2 cut(s) 276, 1892
BsaHI GRCGYC 1 cut(s) 1390
BsaJI CCNNGG 9 cut(s) 873, 889, 959, 1099, 1540, 1710, 1738, 1891, 1900
Bsc4I CCNNNNNNNGG 6 cut(s) 137, 481, 894, 895, 1337, 1711
Bse1I ACTGG 4 cut(s) 142, 467, 584, 2053
Bse3DI GCAATG 3 cut(s) 503, 1675, 1727
BseBI CCWGG 8 cut(s) 237, 412, 747, 875, 961, 1124, 1338, 1740
BseDI CCNNGG 9 cut(s) 873, 889, 959, 1099, 1540, 1710, 1738, 1891, 1900
BseGI GGATG 5 cut(s) 899, 1124, 1342, 2023, 2104
BseLI CCNNNNNNNGG 6 cut(s) 137, 481, 894, 895, 1337, 1711
BseMI GCAATG 3 cut(s) 503, 1675, 1727
BseMII CTCAG 5 cut(s) 179, 186, 576, 1773, 1953
BseNI ACTGG 4 cut(s) 142, 467, 584, 2053
BseRI GAGGAG 2 cut(s) 1085, 1927
BseSI GKGCMC 2 cut(s) 319, 1647
BseXI GCAGC 7 cut(s) 1000, 1277, 1517, 1569, 1573, 1946, 1974
Bsh1236I CGCG 2 cut(s) 585, 643
BshFI GGCC 4 cut(s) 754, 1004, 1743, 1932
BshNI GGYRCC 1 cut(s) 1389
BsiHKAI GWGCWC 4 cut(s) 56, 158, 319, 1779
BsiSI CCGG 2 cut(s) 276, 1891
BslFI GGGAC 2 cut(s) 841, 2119
BslI CCNNNNNNNGG 6 cut(s) 137, 481, 894, 895, 1337, 1711
BsmAI GTCTC 4 cut(s) 565, 821, 1097, 1470
BsmBI CGTCTC 1 cut(s) 1470
BsmFI GGGAC 2 cut(s) 841, 2119
BsnI GGCC 4 cut(s) 754, 1004, 1743, 1932
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 5 cut(s) 56, 158, 319, 1647, 1779
Bsp143I GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
Bsp19I CCATGG 1 cut(s) 1540
BspACI CCGC 4 cut(s) 18, 755, 1296, 1705
BspANI GGCC 4 cut(s) 754, 1004, 1743, 1932
BspCNI CTCAG 5 cut(s) 178, 187, 575, 1772, 1952
BspFNI CGCG 2 cut(s) 585, 643
BspHI TCATGA 1 cut(s) 1414
BspLI GGNNCC 5 cut(s) 416, 990, 1391, 1790, 1897
BspMAI CTGCAG 3 cut(s) 1018, 1507, 1964
BspMI ACCTGC 2 cut(s) 1097, 1538
BspPI GGATC 7 cut(s) 485, 626, 801, 983, 996, 1783, 1796
BspQI GCTCTTC 1 cut(s) 1356
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 1389
BspTI CTTAAG 1 cut(s) 1280
BsrDI GCAATG 3 cut(s) 503, 1675, 1727
BsrI ACTGG 4 cut(s) 142, 467, 584, 2053
BssECI CCNNGG 9 cut(s) 873, 889, 959, 1099, 1540, 1710, 1738, 1891, 1900
BssMI GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
BssNAI GTATAC 1 cut(s) 1221
BssNI GRCGYC 1 cut(s) 1390
BssSI CACGAG 1 cut(s) 831
BssT1I CCWWGG 5 cut(s) 889, 1099, 1540, 1710, 1900
Bst1107I GTATAC 1 cut(s) 1221
Bst2BI CACGAG 1 cut(s) 831
Bst2UI CCWGG 8 cut(s) 237, 412, 747, 875, 961, 1124, 1338, 1740
Bst4CI ACNGT 8 cut(s) 45, 301, 883, 1235, 1277, 1729, 1908, 1946
Bst6I CTCTTC 3 cut(s) 192, 1356, 1473
BstACI GRCGYC 1 cut(s) 1390
BstAFI CTTAAG 1 cut(s) 1280
BstAPI GCANNNNNTGC 2 cut(s) 1372, 1956
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 6 cut(s) 645, 771, 1047, 1132, 1136, 1957
BstDEI CTNAG 6 cut(s) 165, 195, 562, 1759, 1780, 1939
BstDSI CCRYGG 1 cut(s) 1540
BstF5I GGATG 5 cut(s) 899, 1124, 1342, 2023, 2104
BstFNI CGCG 2 cut(s) 585, 643
BstH2I RGCGCY 1 cut(s) 1393
BstHHI GCGC 4 cut(s) 151, 585, 1392, 1539
BstKTI GATC 9 cut(s) 493, 634, 697, 796, 847, 904, 991, 1609, 1791
BstMAI GTCTC 4 cut(s) 565, 821, 1097, 1470
BstMBI GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
BstNI CCWGG 8 cut(s) 237, 412, 747, 875, 961, 1124, 1338, 1740
BstNSI RCATGY 1 cut(s) 1138
BstSFI CTRYAG 5 cut(s) 600, 820, 1014, 1503, 1960
BstSLI GKGCMC 2 cut(s) 319, 1647
BstUI CGCG 2 cut(s) 585, 643
BstV1I GCAGC 7 cut(s) 1000, 1277, 1517, 1569, 1573, 1946, 1974
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 5 cut(s) 793, 844, 901, 988, 1788
BstXI CCANNNNNNTGG 2 cut(s) 925, 2035
BstYI RGATCY 5 cut(s) 793, 844, 901, 988, 1788
BstZ17I GTATAC 1 cut(s) 1221
BsuRI GGCC 4 cut(s) 754, 1004, 1743, 1932
BtgI CCRYGG 1 cut(s) 1540
BtrI CACGTC 1 cut(s) 2044
BtsCI GGATG 5 cut(s) 899, 1124, 1342, 2023, 2104
BtsI GCAGTG 2 cut(s) 930, 1861
BtsIMutI CAGTG 6 cut(s) 135, 930, 1602, 1622, 1725, 1861
BveI ACCTGC 2 cut(s) 1097, 1538
Cac8I GCNNGC 6 cut(s) 645, 771, 1047, 1132, 1136, 1957
CaiI CAGNNNCTG 1 cut(s) 557
CciI TCATGA 1 cut(s) 1414
CfoI GCGC 4 cut(s) 151, 585, 1392, 1539
Cfr13I GGNCC 3 cut(s) 1550, 1714, 1930
Csp6I GTAC 2 cut(s) 404, 1110
CviAII CATG 9 cut(s) 726, 1135, 1321, 1397, 1415, 1541, 1554, 1568, 2078
CviQI GTAC 2 cut(s) 404, 1110
DdeI CTNAG 6 cut(s) 165, 195, 562, 1759, 1780, 1939
DinI GGCGCC 1 cut(s) 1391
DpnI GATC 9 cut(s) 492, 633, 696, 795, 846, 903, 990, 1608, 1790
DpnII GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
DraI TTTAAA 1 cut(s) 1255
EaeI YGGCCR 2 cut(s) 752, 1002
Eam1104I CTCTTC 3 cut(s) 192, 1356, 1473
EarI CTCTTC 3 cut(s) 192, 1356, 1473
Ecl136II GAGCTC 2 cut(s) 54, 1777
Eco130I CCWWGG 5 cut(s) 889, 1099, 1540, 1710, 1900
Eco147I AGGCCT 1 cut(s) 1743
Eco24I GRGCYC 2 cut(s) 56, 1779
Eco47I GGWCC 2 cut(s) 1550, 1714
Eco53kI GAGCTC 2 cut(s) 54, 1777
Eco57I CTGAAG 1 cut(s) 1602
EcoICRI GAGCTC 2 cut(s) 54, 1777
EcoRII CCWGG 8 cut(s) 235, 410, 745, 873, 959, 1122, 1336, 1738
EcoT14I CCWWGG 5 cut(s) 889, 1099, 1540, 1710, 1900
EcoT22I ATGCAT 1 cut(s) 2083
EcoT38I GRGCYC 2 cut(s) 56, 1779
EgeI GGCGCC 1 cut(s) 1391
EheI GGCGCC 1 cut(s) 1391
ErhI CCWWGG 5 cut(s) 889, 1099, 1540, 1710, 1900
Esp3I CGTCTC 1 cut(s) 1470
FaeI CATG 9 cut(s) 729, 1138, 1324, 1400, 1418, 1544, 1557, 1571, 2081
FaqI GGGAC 2 cut(s) 841, 2119
FatI CATG 9 cut(s) 725, 1134, 1320, 1396, 1414, 1540, 1553, 1567, 2077
FauI CCCGC 1 cut(s) 1303
FbaI TGATCA 1 cut(s) 694
FblI GTMKAC 2 cut(s) 60, 1220
Fnu4HI GCNGC 9 cut(s) 19, 755, 1014, 1266, 1506, 1558, 1587, 1960, 1963
FokI GGATG 5 cut(s) 906, 1131, 1329, 2010, 2111
FriOI GRGCYC 2 cut(s) 56, 1779
Fsp4HI GCNGC 9 cut(s) 19, 755, 1014, 1266, 1506, 1558, 1587, 1960, 1963
FspBI CTAG 6 cut(s) 191, 848, 996, 1353, 1563, 1839
GlaI GCGC 4 cut(s) 150, 584, 1391, 1538
GluI GCNGC 9 cut(s) 19, 755, 1014, 1266, 1506, 1558, 1587, 1960, 1963
GsuI CTGGAG 2 cut(s) 480, 2057
HaeII RGCGCY 1 cut(s) 1393
HaeIII GGCC 4 cut(s) 754, 1004, 1743, 1932
HapII CCGG 2 cut(s) 276, 1891
HhaI GCGC 4 cut(s) 151, 585, 1392, 1539
Hin1I GRCGYC 1 cut(s) 1390
Hin1II CATG 9 cut(s) 729, 1138, 1324, 1400, 1418, 1544, 1557, 1571, 2081
Hin6I GCGC 4 cut(s) 149, 583, 1390, 1537
HinP1I GCGC 4 cut(s) 149, 583, 1390, 1537
HincII GTYRAC 4 cut(s) 61, 1174, 1273, 1434
HindII GTYRAC 4 cut(s) 61, 1174, 1273, 1434
HindIII AAGCTT 2 cut(s) 384, 592
HpaI GTTAAC 2 cut(s) 1273, 1434
HpaII CCGG 2 cut(s) 276, 1891
HphI GGTGA 4 cut(s) 878, 1259, 1790, 1835
Hpy166II GTNNAC 8 cut(s) 61, 233, 317, 1112, 1174, 1221, 1273, 1434
Hpy188I TCNGA 9 cut(s) 51, 211, 375, 762, 1158, 1582, 1611, 1942, 1978
Hpy188III TCNNGA 4 cut(s) 786, 1358, 1415, 2036
Hpy8I GTNNAC 8 cut(s) 61, 233, 317, 1112, 1174, 1221, 1273, 1434
Hpy99I CGWCG 3 cut(s) 62, 65, 119
HpyAV CCTTC 6 cut(s) 216, 299, 481, 538, 1065, 1814
HpyCH4III ACNGT 8 cut(s) 45, 301, 883, 1235, 1277, 1729, 1908, 1946
HpyCH4IV ACGT 2 cut(s) 1166, 2043
HpyF3I CTNAG 6 cut(s) 165, 195, 562, 1759, 1780, 1939
HpySE526I ACGT 2 cut(s) 1166, 2043
Hsp92I GRCGYC 1 cut(s) 1390
Hsp92II CATG 9 cut(s) 729, 1138, 1324, 1400, 1418, 1544, 1557, 1571, 2081
HspAI GCGC 4 cut(s) 149, 583, 1390, 1537
KasI GGCGCC 1 cut(s) 1389
Ksp22I TGATCA 1 cut(s) 694
KspAI GTTAAC 2 cut(s) 1273, 1434
Kzo9I GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
LguI GCTCTTC 1 cut(s) 1356
LmnI GCTCC 6 cut(s) 59, 272, 450, 1103, 1544, 1753
Lsp1109I GCAGC 7 cut(s) 1000, 1277, 1517, 1569, 1573, 1946, 1974
LweI GCATC 5 cut(s) 120, 254, 667, 1109, 1408
MaeI CTAG 6 cut(s) 191, 848, 996, 1353, 1563, 1839
MaeII ACGT 2 cut(s) 1166, 2043
MaeIII GTNAC 3 cut(s) 877, 1729, 2044
MalI GATC 9 cut(s) 492, 633, 696, 795, 846, 903, 990, 1608, 1790
MboI GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
MboII GAAGA 8 cut(s) 22, 78, 179, 233, 725, 896, 1373, 1460
MflI RGATCY 5 cut(s) 793, 844, 901, 988, 1788
MhlI GDGCHC 5 cut(s) 56, 158, 319, 1647, 1779
Mly113I GGCGCC 1 cut(s) 1390
MlyI GAGTC 6 cut(s) 113, 178, 206, 581, 823, 1565
MmeI TCCRAC 1 cut(s) 1016
Mph1103I ATGCAT 1 cut(s) 2083
MroXI GAANNNNTTC 1 cut(s) 309
MslI CAYNNNNRTG 4 cut(s) 1117, 1139, 1803, 1970
MspA1I CMGCKG 1 cut(s) 1298
MspCI CTTAAG 1 cut(s) 1280
MspI CCGG 2 cut(s) 276, 1891
MvaI CCWGG 8 cut(s) 237, 412, 747, 875, 961, 1124, 1338, 1740
MvnI CGCG 2 cut(s) 585, 643
NarI GGCGCC 1 cut(s) 1390
NciI CCSGG 2 cut(s) 276, 1892
NcoI CCATGG 1 cut(s) 1540
NdeII GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
NlaIII CATG 9 cut(s) 729, 1138, 1324, 1400, 1418, 1544, 1557, 1571, 2081
NlaIV GGNNCC 5 cut(s) 416, 990, 1391, 1790, 1897
NmuCI GTSAC 1 cut(s) 2044
NsiI ATGCAT 1 cut(s) 2083
NspI RCATGY 1 cut(s) 1138
NspV TTCGAA 1 cut(s) 215
PaeI GCATGC 1 cut(s) 1138
PagI TCATGA 1 cut(s) 1414
PceI AGGCCT 1 cut(s) 1743
PciSI GCTCTTC 1 cut(s) 1356
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 7 cut(s) 212, 224, 244, 554, 742, 1409, 1691
PflMI CCANNNNNTGG 1 cut(s) 1337
PkrI GCNGC 9 cut(s) 20, 756, 1015, 1267, 1507, 1559, 1588, 1961, 1964
PleI GAGTC 6 cut(s) 113, 178, 205, 581, 823, 1565
PluTI GGCGCC 1 cut(s) 1393
PpsI GAGTC 6 cut(s) 113, 178, 205, 581, 823, 1565
Psp124BI GAGCTC 2 cut(s) 56, 1779
Psp6I CCWGG 8 cut(s) 235, 410, 745, 873, 959, 1122, 1336, 1738
PspGI CCWGG 8 cut(s) 235, 410, 745, 873, 959, 1122, 1336, 1738
PspN4I GGNNCC 5 cut(s) 416, 990, 1391, 1790, 1897
PspPI GGNCC 3 cut(s) 1550, 1714, 1930
PstI CTGCAG 3 cut(s) 1018, 1507, 1964
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 5 cut(s) 793, 844, 901, 988, 1788
RsaI GTAC 2 cut(s) 405, 1111
RsaNI GTAC 2 cut(s) 404, 1110
RseI CAYNNNNRTG 4 cut(s) 1117, 1139, 1803, 1970
SacI GAGCTC 2 cut(s) 56, 1779
SalI GTCGAC 1 cut(s) 59
SapI GCTCTTC 1 cut(s) 1356
SatI GCNGC 9 cut(s) 19, 755, 1014, 1266, 1506, 1558, 1587, 1960, 1963
Sau3AI GATC 9 cut(s) 490, 631, 694, 793, 844, 901, 988, 1606, 1788
Sau96I GGNCC 3 cut(s) 1550, 1714, 1930
SchI GAGTC 6 cut(s) 113, 178, 206, 581, 823, 1565
SduI GDGCHC 5 cut(s) 56, 158, 319, 1647, 1779
SfaNI GCATC 5 cut(s) 120, 254, 667, 1109, 1408
SfcI CTRYAG 5 cut(s) 600, 820, 1014, 1503, 1960
SfoI GGCGCC 1 cut(s) 1391
SfuI TTCGAA 1 cut(s) 215
SgrDI CGTCGACG 1 cut(s) 59
SinI GGWCC 2 cut(s) 1550, 1714
SmiMI CAYNNNNRTG 4 cut(s) 1117, 1139, 1803, 1970
SmlI CTYRAG 7 cut(s) 589, 784, 797, 1061, 1085, 1204, 1280
SmoI CTYRAG 7 cut(s) 589, 784, 797, 1061, 1085, 1204, 1280
SphI GCATGC 1 cut(s) 1138
SseBI AGGCCT 1 cut(s) 1743
SsiI CCGC 4 cut(s) 18, 755, 1296, 1705
SspDI GGCGCC 1 cut(s) 1389
SspI AATATT 1 cut(s) 1290
SspMI CTAG 6 cut(s) 191, 848, 996, 1353, 1563, 1839
SstI GAGCTC 2 cut(s) 56, 1779
StuI AGGCCT 1 cut(s) 1743
StyI CCWWGG 5 cut(s) 889, 1099, 1540, 1710, 1900
TaaI ACNGT 8 cut(s) 45, 301, 883, 1235, 1277, 1729, 1908, 1946
TaiI ACGT 2 cut(s) 1169, 2046
TaqI TCGA 5 cut(s) 60, 114, 215, 227, 1984
TatI WGTACW 1 cut(s) 403
TauI GCSGC 2 cut(s) 21, 757
TfiI GAWTC 7 cut(s) 212, 224, 244, 554, 742, 1409, 1691
TscAI CASTG 6 cut(s) 142, 930, 1609, 1629, 1732, 1861
TseFI GTSAC 1 cut(s) 2044
TseI GCWGC 7 cut(s) 1013, 1265, 1505, 1557, 1586, 1959, 1962
Tsp45I GTSAC 1 cut(s) 2044
TspDTI ATGAA 4 cut(s) 234, 446, 896, 2081
TspGWI ACGGA 4 cut(s) 46, 259, 1452, 2012
TspRI CASTG 6 cut(s) 142, 930, 1609, 1629, 1732, 1861
Van91I CCANNNNNTGG 1 cut(s) 1337
Vha464I CTTAAG 1 cut(s) 1280
VneI GTGCAC 1 cut(s) 315
VpaK11BI GGWCC 2 cut(s) 1550, 1714
XapI RAATTY 5 cut(s) 637, 1024, 1250, 2000, 2136
XceI RCATGY 1 cut(s) 1138
XcmI CCANNNNNNNNNTGG 1 cut(s) 1708
XmiI GTMKAC 2 cut(s) 60, 1220
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 6 cut(s) 191, 848, 996, 1353, 1563, 1839
Zsp2I ATGCAT 1 cut(s) 2083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.