DNA recombination
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 87 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 457 |
| AccI | GTMKAC | 2 cut(s) | 87, 219 |
| AccIII | TCCGGA | 1 cut(s) | 160 |
| AciI | CCGC | 2 cut(s) | 61, 93 |
| AclWI | GGATC | 3 cut(s) | 95, 152, 373 |
| AcoI | YGGCCR | 1 cut(s) | 232 |
| AcsI | RAATTY | 1 cut(s) | 149 |
| AcyI | GRCGYC | 2 cut(s) | 84, 90 |
| AfaI | GTAC | 1 cut(s) | 173 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| AgsI | TTSAA | 1 cut(s) | 403 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 9 |
| AjnI | CCWGG | 2 cut(s) | 361, 429 |
| AluBI | AGCT | 2 cut(s) | 187, 229 |
| AluI | AGCT | 2 cut(s) | 187, 229 |
| Alw21I | GWGCWC | 1 cut(s) | 39 |
| Alw44I | GTGCAC | 1 cut(s) | 35 |
| AlwI | GGATC | 3 cut(s) | 95, 152, 373 |
| Aor13HI | TCCGGA | 1 cut(s) | 160 |
| AoxI | GGCC | 1 cut(s) | 232 |
| ApaLI | GTGCAC | 1 cut(s) | 35 |
| ApeKI | GCWGC | 3 cut(s) | 184, 422, 425 |
| ApoI | RAATTY | 1 cut(s) | 149 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 72 |
| AsuC2I | CCSGG | 1 cut(s) | 29 |
| AsuHPI | GGTGA | 1 cut(s) | 205 |
| BaeGI | GKGCMC | 1 cut(s) | 39 |
| Bbv12I | GWGCWC | 1 cut(s) | 39 |
| BbvI | GCAGC | 3 cut(s) | 196, 409, 412 |
| BccI | CCATC | 1 cut(s) | 218 |
| BciT130I | CCWGG | 2 cut(s) | 363, 431 |
| BclI | TGATCA | 1 cut(s) | 15 |
| BcnI | CCSGG | 1 cut(s) | 29 |
| BglII | AGATCT | 1 cut(s) | 70 |
| BisI | GCNGC | 4 cut(s) | 93, 185, 423, 426 |
| BlpI | GCTNAGC | 1 cut(s) | 188 |
| BlsI | GCNGC | 4 cut(s) | 94, 186, 424, 427 |
| Bme1390I | CCNGG | 3 cut(s) | 29, 363, 431 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 9 |
| BmrFI | CCNGG | 3 cut(s) | 29, 363, 431 |
| BmsI | GCATC | 1 cut(s) | 175 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 297, 329 |
| BpmI | CTGGAG | 2 cut(s) | 345, 452 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 188 |
| BpuEI | CTTGAG | 1 cut(s) | 299 |
| BpuMI | CCSGG | 1 cut(s) | 29 |
| BsaHI | GRCGYC | 2 cut(s) | 84, 90 |
| BsaJI | CCNNGG | 2 cut(s) | 201, 429 |
| BsaWI | WCCGGW | 1 cut(s) | 160 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 155, 185 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| Bse1I | ACTGG | 1 cut(s) | 477 |
| Bse3DI | GCAATG | 2 cut(s) | 268, 326 |
| BseAI | TCCGGA | 1 cut(s) | 160 |
| BseBI | CCWGG | 2 cut(s) | 363, 431 |
| BseDI | CCNNGG | 2 cut(s) | 201, 429 |
| BseGI | GGATG | 2 cut(s) | 166, 466 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| BseMI | GCAATG | 2 cut(s) | 268, 326 |
| BseMII | CTCAG | 2 cut(s) | 202, 293 |
| BseNI | ACTGG | 1 cut(s) | 477 |
| BseRI | GAGGAG | 2 cut(s) | 218, 321 |
| BseSI | GKGCMC | 1 cut(s) | 39 |
| BseX3I | CGGCCG | 1 cut(s) | 232 |
| BseXI | GCAGC | 3 cut(s) | 196, 409, 412 |
| BseYI | CCCAGC | 1 cut(s) | 7 |
| Bsh1285I | CGRYCG | 1 cut(s) | 235 |
| BshFI | GGCC | 1 cut(s) | 234 |
| BsiEI | CGRYCG | 1 cut(s) | 235 |
| BsiHKAI | GWGCWC | 1 cut(s) | 39 |
| BsiSI | CCGG | 3 cut(s) | 29, 161, 235 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 160, 457 |
| BsnI | GGCC | 1 cut(s) | 234 |
| Bsp1286I | GDGCHC | 1 cut(s) | 39 |
| Bsp13I | TCCGGA | 1 cut(s) | 160 |
| Bsp143I | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 188 |
| BspACI | CCGC | 2 cut(s) | 61, 93 |
| BspANI | GGCC | 1 cut(s) | 234 |
| BspCNI | CTCAG | 2 cut(s) | 201, 294 |
| BspEI | TCCGGA | 1 cut(s) | 160 |
| BspPI | GGATC | 3 cut(s) | 95, 152, 373 |
| BsrDI | GCAATG | 2 cut(s) | 268, 326 |
| BsrI | ACTGG | 1 cut(s) | 477 |
| BssECI | CCNNGG | 2 cut(s) | 201, 429 |
| BssMI | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| BssNI | GRCGYC | 2 cut(s) | 84, 90 |
| Bst2UI | CCWGG | 2 cut(s) | 363, 431 |
| Bst4CI | ACNGT | 3 cut(s) | 217, 286, 486 |
| BstACI | GRCGYC | 2 cut(s) | 84, 90 |
| BstC8I | GCNNGC | 3 cut(s) | 39, 61, 194 |
| BstDEI | CTNAG | 3 cut(s) | 42, 188, 302 |
| BstF5I | GGATG | 2 cut(s) | 166, 466 |
| BstKTI | GATC | 6 cut(s) | 18, 73, 103, 137, 160, 368 |
| BstMBI | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| BstMCI | CGRYCG | 1 cut(s) | 235 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 193 |
| BstNI | CCWGG | 2 cut(s) | 363, 431 |
| BstSCI | CCNGG | 3 cut(s) | 27, 361, 429 |
| BstSLI | GKGCMC | 1 cut(s) | 39 |
| BstV1I | GCAGC | 3 cut(s) | 196, 409, 412 |
| BstX2I | RGATCY | 3 cut(s) | 70, 157, 365 |
| BstYI | RGATCY | 3 cut(s) | 70, 157, 365 |
| BstZI | CGGCCG | 1 cut(s) | 232 |
| BsuRI | GGCC | 1 cut(s) | 234 |
| BtsCI | GGATG | 2 cut(s) | 166, 466 |
| BtsIMutI | CAGTG | 2 cut(s) | 291, 484 |
| Cac8I | GCNNGC | 3 cut(s) | 39, 61, 194 |
| CseI | GACGC | 2 cut(s) | 13, 98 |
| Csp6I | GTAC | 1 cut(s) | 172 |
| CviAII | CATG | 1 cut(s) | 371 |
| CviJI | RGCY | 3 cut(s) | 187, 229, 234 |
| CviKI_1 | RGCY | 3 cut(s) | 187, 229, 234 |
| CviQI | GTAC | 1 cut(s) | 172 |
| DdeI | CTNAG | 3 cut(s) | 42, 188, 302 |
| DpnI | GATC | 6 cut(s) | 17, 72, 102, 136, 159, 367 |
| DpnII | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| DraI | TTTAAA | 1 cut(s) | 148 |
| DriI | GACNNNNNGTC | 1 cut(s) | 9 |
| EaeI | YGGCCR | 1 cut(s) | 232 |
| EagI | CGGCCG | 1 cut(s) | 232 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 9 |
| EclXI | CGGCCG | 1 cut(s) | 232 |
| Eco32I | GATATC | 1 cut(s) | 121 |
| Eco52I | CGGCCG | 1 cut(s) | 232 |
| EcoRII | CCWGG | 2 cut(s) | 361, 429 |
| EcoRV | GATATC | 1 cut(s) | 121 |
| FaeI | CATG | 1 cut(s) | 374 |
| FaiI | YATR | 4 cut(s) | 117, 278, 298, 372 |
| FatI | CATG | 1 cut(s) | 370 |
| FauI | CCCGC | 1 cut(s) | 68 |
| FbaI | TGATCA | 1 cut(s) | 15 |
| FblI | GTMKAC | 2 cut(s) | 87, 219 |
| Fnu4HI | GCNGC | 4 cut(s) | 93, 185, 423, 426 |
| FokI | GGATG | 2 cut(s) | 153, 473 |
| Fsp4HI | GCNGC | 4 cut(s) | 93, 185, 423, 426 |
| GluI | GCNGC | 4 cut(s) | 93, 185, 423, 426 |
| GsaI | CCCAGC | 1 cut(s) | 11 |
| GsuI | CTGGAG | 2 cut(s) | 345, 452 |
| HaeIII | GGCC | 1 cut(s) | 234 |
| HapII | CCGG | 3 cut(s) | 29, 161, 235 |
| HgaI | GACGC | 2 cut(s) | 13, 98 |
| Hin1I | GRCGYC | 2 cut(s) | 84, 90 |
| Hin1II | CATG | 1 cut(s) | 374 |
| HincII | GTYRAC | 3 cut(s) | 88, 220, 346 |
| HindII | GTYRAC | 3 cut(s) | 88, 220, 346 |
| HinfI | GANTC | 4 cut(s) | 206, 238, 383, 436 |
| HpaII | CCGG | 3 cut(s) | 29, 161, 235 |
| HphI | GGTGA | 1 cut(s) | 205 |
| Hpy166II | GTNNAC | 6 cut(s) | 37, 88, 220, 252, 346, 482 |
| Hpy188I | TCNGA | 5 cut(s) | 15, 82, 155, 202, 243 |
| Hpy188III | TCNNGA | 3 cut(s) | 107, 132, 161 |
| Hpy8I | GTNNAC | 6 cut(s) | 37, 88, 220, 252, 346, 482 |
| Hpy99I | CGWCG | 5 cut(s) | 86, 89, 92, 221, 224 |
| HpyAV | CCTTC | 2 cut(s) | 40, 455 |
| HpyCH4III | ACNGT | 3 cut(s) | 217, 286, 486 |
| HpyCH4IV | ACGT | 2 cut(s) | 84, 248 |
| HpyCH4V | TGCA | 3 cut(s) | 37, 273, 374 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 193 |
| HpyF3I | CTNAG | 3 cut(s) | 42, 188, 302 |
| HpySE526I | ACGT | 2 cut(s) | 84, 248 |
| Hsp92I | GRCGYC | 2 cut(s) | 84, 90 |
| Hsp92II | CATG | 1 cut(s) | 374 |
| Kpn2I | TCCGGA | 1 cut(s) | 160 |
| Ksp22I | TGATCA | 1 cut(s) | 15 |
| Kzo9I | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| LmnI | GCTCC | 2 cut(s) | 163, 181 |
| LpnPI | CCDG | 8 cut(s) | 42, 174, 248, 314, 348, 375, 416, 443 |
| Lsp1109I | GCAGC | 3 cut(s) | 196, 409, 412 |
| LweI | GCATC | 1 cut(s) | 175 |
| MaeII | ACGT | 2 cut(s) | 84, 248 |
| MalI | GATC | 6 cut(s) | 17, 72, 102, 136, 159, 367 |
| MboI | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| MboII | GAAGA | 3 cut(s) | 65, 80, 392 |
| MflI | RGATCY | 3 cut(s) | 70, 157, 365 |
| MhlI | GDGCHC | 1 cut(s) | 39 |
| MluCI | AATT | 1 cut(s) | 149 |
| MlyI | GAGTC | 1 cut(s) | 215 |
| MmeI | TCCRAC | 2 cut(s) | 105, 159 |
| MnlI | CCTC | 5 cut(s) | 114, 196, 339, 342, 479 |
| MroI | TCCGGA | 1 cut(s) | 160 |
| MroXI | GAANNNNTTC | 1 cut(s) | 72 |
| MseI | TTAA | 4 cut(s) | 147, 309, 351, 390 |
| MslI | CAYNNNNRTG | 1 cut(s) | 375 |
| MspA1I | CMGCKG | 1 cut(s) | 95 |
| MspI | CCGG | 3 cut(s) | 29, 161, 235 |
| MspR9I | CCNGG | 3 cut(s) | 29, 363, 431 |
| MvaI | CCWGG | 2 cut(s) | 363, 431 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 193 |
| NciI | CCSGG | 1 cut(s) | 29 |
| NdeII | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| NlaIII | CATG | 1 cut(s) | 374 |
| NmeAIII | GCCGAG | 1 cut(s) | 210 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 254 |
| PdmI | GAANNNNTTC | 1 cut(s) | 72 |
| PfeI | GAWTC | 3 cut(s) | 238, 383, 436 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 457 |
| PfoI | TCCNGGA | 1 cut(s) | 361 |
| PkrI | GCNGC | 4 cut(s) | 94, 186, 424, 427 |
| PleI | GAGTC | 1 cut(s) | 214 |
| PpsI | GAGTC | 1 cut(s) | 214 |
| Psp6I | CCWGG | 2 cut(s) | 361, 429 |
| PspFI | CCCAGC | 1 cut(s) | 7 |
| PspGI | CCWGG | 2 cut(s) | 361, 429 |
| PsuI | RGATCY | 3 cut(s) | 70, 157, 365 |
| RsaI | GTAC | 1 cut(s) | 173 |
| RsaNI | GTAC | 1 cut(s) | 172 |
| RseI | CAYNNNNRTG | 1 cut(s) | 375 |
| SalI | GTCGAC | 2 cut(s) | 86, 218 |
| SaqAI | TTAA | 4 cut(s) | 147, 309, 351, 390 |
| SatI | GCNGC | 4 cut(s) | 93, 185, 423, 426 |
| Sau3AI | GATC | 6 cut(s) | 15, 70, 100, 134, 157, 365 |
| SchI | GAGTC | 1 cut(s) | 215 |
| ScrFI | CCNGG | 3 cut(s) | 29, 363, 431 |
| SduI | GDGCHC | 1 cut(s) | 39 |
| SetI | ASST | 7 cut(s) | 87, 189, 231, 251, 345, 357, 447 |
| SfaNI | GCATC | 1 cut(s) | 175 |
| SgrDI | CGTCGACG | 2 cut(s) | 86, 218 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 375 |
| SmlI | CTYRAG | 1 cut(s) | 314 |
| SmoI | CTYRAG | 1 cut(s) | 314 |
| Sse9I | AATT | 1 cut(s) | 149 |
| SsiI | CCGC | 2 cut(s) | 61, 93 |
| StyD4I | CCNGG | 3 cut(s) | 27, 361, 429 |
| TaaI | ACNGT | 3 cut(s) | 217, 286, 486 |
| TaiI | ACGT | 2 cut(s) | 87, 251 |
| TaqI | TCGA | 4 cut(s) | 87, 108, 133, 219 |
| TasI | AATT | 1 cut(s) | 149 |
| TauI | GCSGC | 1 cut(s) | 95 |
| TfiI | GAWTC | 3 cut(s) | 238, 383, 436 |
| Tru1I | TTAA | 4 cut(s) | 147, 309, 351, 390 |
| Tru9I | TTAA | 4 cut(s) | 147, 309, 351, 390 |
| TscAI | CASTG | 2 cut(s) | 291, 484 |
| TseI | GCWGC | 3 cut(s) | 184, 422, 425 |
| TspDTI | ATGAA | 1 cut(s) | 393 |
| TspGWI | ACGGA | 1 cut(s) | 159 |
| TspRI | CASTG | 2 cut(s) | 291, 484 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 457 |
| VneI | GTGCAC | 1 cut(s) | 35 |
| XapI | RAATTY | 1 cut(s) | 149 |
| XmiI | GTMKAC | 2 cut(s) | 87, 219 |
| XmnI | GAANNNNTTC | 1 cut(s) | 72 |
| ZraI | GACGTC | 1 cut(s) | 85 |