FvH4_2g06412

G-quadruplex DNA unwinding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000050
Physical Location & Seq
Reverse (-)
5333093 .. 5335529
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06412.t1

Sequence Viewer

Length: 702 bp
ATGTGGTTTCATTTAAATGATGAACGGGAGTCGGCCAAATATTTATATCCAACTGATGCATTCACTTTTACAGGAAGGAAAGACGATGGACATAACACCGGTGAGAGCAATGGTACCCTTCAGGTGGAGACAAAATGTGAAATCACTGTTCAAAACCTGAGAAGGGAAACCCTCAACATCACTCTTTGGGGAGACATCGCCAGGTCATTTGACATTGGAACTGTGCAAAGATCATCTTCTCCTGTTCTTGCTGTGTTTACCAGCTTGCTTCTAGGAGAATATAATGAACATGTTGTGGCCTCAAACTCAAACCATACATGTGTTTTCTTCAATCCACCCATTCCCGCCAGAGGCGAGTACATGCTCGAATTCTCCAAAATAGGAGACAAGGTGCGCATAATCGGTGCCATCTCCAGCCATCAAACAACTAAATGTGTTGAACAACCAAACCAAGTGACTGTGTCGGAGTTGAATGCACTTAATCCAATAGATTACATGGAGAAGCTGGTATTCTGCAGTGCTTCGATTCGAAGATTCTCACCAAATGGATGGTGGTATAGAAGCTGCTCAACCACCGGCTGTTACAGGATGCTGCACAAAAATGAGGAGAAGGACAATAAATACTTTTGTCCGTTGCATAGTCTTCAGAACCCGCAACCATCCTATGTGGTCAGGATGACAATTGAGGACGAAGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Helitron_like_N Pkinase Pkinase_Tyr REPA_OB_2 WW

Protein Analysis

234

Amino Acids

26.64

Weight (kDa)

5.82

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000050)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_1g07041 FvH4_2g06412 FvH4_2g08146 FvH4_2g10321 FvH4_2g13511 FvH4_2g20780 FvH4_2g20781 FvH4_2g22171 FvH4_2g25221 FvH4_3g03500 FvH4_3g07130 FvH4_3g10140 FvH4_3g10141 FvH4_3g24350 FvH4_4g08242 FvH4_4g13410 FvH4_4g18140 FvH4_4g22470 FvH4_5g19920 FvH4_5g21501 FvH4_5g26612 FvH4_5g29621 FvH4_5g31990 FvH4_6g00280 FvH4_6g00280 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g07190 FvH4_6g08820 FvH4_6g29600 FvH4_6g30401 FvH4_6g33630 FvH4_7g08310 FvH4_7g08311 FvH4_7g10871 FvH4_7g18890 FvH4_7g18891
malus_domestica MD01G1202900.v1.1 MD02G1249900.v1.1 MD03G1022200.v1.1 MD03G1164900.v1.1 MD04G1002000.v1.1 MD05G1167800.v1.1 MD05G1167900.v1.1 MD05G1202200.v1.1 MD05G1215800.v1.1 MD05G1328800.v1.1 MD07G1020000.v1.1 MD07G1020100.v1.1 MD07G1084700.v1.1 MD08G1007900.v1.1 MD08G1229500.v1.1 MD09G1016000.v1.1 MD09G1184200.v1.1 MD10G1198800.v1.1 MD10G1198900.v1.1 MD11G1203900.v1.1 MD12G1081100.v1.1 MD12G1101700.v1.1 MD13G1126700.v1.1 MD13G1158300.v1.1 MD13G1161500.v1.1 MD14G1083800.v1.1 MD14G1125700.v1.1 MD14G1159700.v1.1 MD14G1159800.v1.1 MD17G1252600.v1.1 MD17G1261900.v1.1 MD17G1262000.v1.1
pyrus_communis pycom01g12270 pycom02g14690 pycom02g17560 pycom02g26880 pycom02g26890 pycom03g13090 pycom03g19990 pycom03g20000 pycom04g00130 pycom04g05050 pycom04g10460 pycom05g27190 pycom07g05970 pycom08g02820 pycom08g11570 pycom09g07420 pycom09g07430 pycom09g08580 pycom10g06760 pycom10g11210 pycom10g11220 pycom11g14380 pycom11g21990 pycom12419g00230 pycom12494g00020 pycom14g06150 pycom15g09750 pycom15g20130 pycom15g27020 pycom15g27230 pycom16g17550 pycom16g18200 pycom5153684g00260 pycom520g00040
rosa_chinensis RchiOBHm_Chr1g0320331 RchiOBHm_Chr1g0329871 RchiOBHm_Chr1g0339991 RchiOBHm_Chr1g0371171 RchiOBHm_Chr1g0378741 RchiOBHm_Chr1g0378751 RchiOBHm_Chr1g0379271 RchiOBHm_Chr2g0122381 RchiOBHm_Chr2g0133251 RchiOBHm_Chr2g0133271 RchiOBHm_Chr2g0133331 RchiOBHm_Chr2g0135141 RchiOBHm_Chr2g0141711 RchiOBHm_Chr2g0145531 RchiOBHm_Chr2g0149141 RchiOBHm_Chr2g0149151 RchiOBHm_Chr2g0150581 RchiOBHm_Chr2g0159361 RchiOBHm_Chr2g0162161 RchiOBHm_Chr2g0174211 RchiOBHm_Chr3g0484061 RchiOBHm_Chr3g0491361 RchiOBHm_Chr4g0413141 RchiOBHm_Chr4g0444601 RchiOBHm_Chr5g0004901 RchiOBHm_Chr5g0004911 RchiOBHm_Chr5g0010461 RchiOBHm_Chr5g0027941 RchiOBHm_Chr5g0028311 RchiOBHm_Chr5g0030141 RchiOBHm_Chr5g0035621 RchiOBHm_Chr5g0050871 RchiOBHm_Chr5g0053321 RchiOBHm_Chr5g0060091 RchiOBHm_Chr5g0063301 RchiOBHm_Chr5g0066571 RchiOBHm_Chr5g0067561 RchiOBHm_Chr6g0262741 RchiOBHm_Chr7g0184001 RchiOBHm_Chr7g0206181 RchiOBHm_Chr7g0212031 RchiOBHm_Chr7g0214521 RchiOBHm_Chr7g0230521 RchiOBHm_Chr7g0230621
rosa_laevigata RLG00000014424 RLG00000022069 RLG00000036206
rosa_multiflora Rmu_sc0000121.1_g000006 Rmu_sc0000271.1_g000001 Rmu_sc0000293.1_g000010 Rmu_sc0000309.1_g000052 Rmu_sc0000556.1_g000045 Rmu_sc0000837.1_g000079 Rmu_sc0001069.1_g000026 Rmu_sc0001198.1_g000001 Rmu_sc0001353.1_g000031 Rmu_sc0001461.1_g000107 Rmu_sc0002011.1_g000038 Rmu_sc0003270.1_g000034 Rmu_sc0003874.1_g000017 Rmu_sc0004729.1_g000006 Rmu_sc0007116.1_g000001 Rmu_sc0009087.1_g000019 Rmu_sc0009877.1_g000004 Rmu_sc0015060.1_g000008 Rmu_sc0016097.1_g000001
rosa_roxburghii Rroxscaffold_1G00024140 Rroxscaffold_1G00027240 Rroxscaffold_1G00030710 Rroxscaffold_1G00035010 Rroxscaffold_1G00050490 Rroxscaffold_1G00069340 Rroxscaffold_1G00072870 Rroxscaffold_2G00078360 Rroxscaffold_2G00098490 Rroxscaffold_2G00106110 Rroxscaffold_2G00109450 Rroxscaffold_2G00125260 Rroxscaffold_2G00154260 Rroxscaffold_3G00221290 Rroxscaffold_3G00221300 Rroxscaffold_3G00232770 Rroxscaffold_3G00249670 Rroxscaffold_3G00255640 Rroxscaffold_3G00255820 Rroxscaffold_3G00255870 Rroxscaffold_3G00258160 Rroxscaffold_3G00258170 Rroxscaffold_3G00265290 Rroxscaffold_4G00320550 Rroxscaffold_4G00322930 Rroxscaffold_4G00331930 Rroxscaffold_4G00332460 Rroxscaffold_5G00337730 Rroxscaffold_5G00364590 Rroxscaffold_5G00365660 Rroxscaffold_5G00373900 Rroxscaffold_6G00395750 Rroxscaffold_6G00397090 Rroxscaffold_6G00407660 Rroxscaffold_6G00421590 Rroxscaffold_7G00156820 Rroxscaffold_7G00174040 Rroxscaffold_7G00175610 Rroxscaffold_7G00182780 Rroxscaffold_7G00210390 Rroxscaffold_7G00210490 Rroxscaffold_7G00214770 Rroxscaffold_7G00215580 Rroxscaffold_7G00215690
rosa_rugosa Rorug01G0099600 Rorug01G0127300 Rorug02G0145300 Rorug02G0145400 Rorug02G0145500.1 Rorug02G0145600 Rorug02G0145700 Rorug02G0145700 Rorug02G0278400 Rorug02G0444700 Rorug02G0542800 Rorug02G0547200 Rorug04G0015900 Rorug04G0082400 Rorug04G0297900 Rorug05G0078200 Rorug05G0207900 Rorug05G0277000 Rorug05G0528600 Rorug05G0528700 Rorug05G0528800 Rorug06G0001000 Rorug06G0246500 Rorug06G0246600 Rorug06G0246700 Rorug07G0189200 Rorug07G0189300 Rorug07G0189400 Rorug07G0189500 Rorug07G0212200 Rorug07G0212200 Rorug07G0221500 Rorug07G0248800
rosa_samantha Rh1AG011600 Rh1AG052100 Rh1AG163900 Rh1BG006600 Rh1BG025100 Rh1BG025200 Rh1BG032500 Rh1BG374400 Rh1BG374500 Rh1CG153000 Rh1DG148800 Rh1DG160400 Rh1DG181900 Rh1DG405100 Rh1DG445600 Rh2AG005600 Rh2AG137100 Rh2AG150500 Rh2AG150600 Rh2AG243200 Rh2AG264300 Rh2AG349900 Rh2AG387700 Rh2AG485100 Rh2CG115600 Rh2CG269600 Rh2CG294000 Rh2CG294100 Rh2CG317700 Rh2CG375000 Rh2CG471200 Rh2CG611400 Rh2DG070000 Rh2DG073900 Rh2DG234600 Rh3AG258200 Rh3CG251500 Rh3CG283900 Rh3CG331200 Rh3CG371500 Rh3DG077300 Rh3DG185900 Rh3DG332700 Rh3DG360400 Rh4AG105100 Rh4BG191100 Rh4DG139800 Rh4DG186000 Rh4DG187900 Rh5AG302000 Rh5AG431600 Rh5CG090000 Rh5CG214700 Rh5CG299100 Rh5CG561200 Rh5DG205600 Rh5DG244500 Rh5DG257300 Rh5DG257400 Rh5DG325900 Rh5DG335500 Rh5DG359300 Rh5DG364600 Rh5DG388600 Rh6AG013900 Rh6BG045400 Rh6CG031300 Rh6CG069100 Rh6CG157500 Rh6DG029800 Rh6DG066100 Rh6DG260800 Rh6DG320100 Rh7AG007600 Rh7BG046100 Rh7BG053900 Rh7BG204900 Rh7BG322600 Rh7BG421200 Rh7DG063200 Rh7DG233200 Rh7DG287000 Rh7DG289600 Rh7DG323500 Rh7DG323800 Rh7DG323900
rosa_wichuraiana Rw0G001160 Rw0G003580 Rw0G003590 Rw0G005910 Rw0G006230 Rw0G010240 Rw0G011660 Rw0G015630 Rw0G021440 Rw0G023690 Rw1G000060 Rw1G006170 Rw1G007940 Rw1G008630 Rw1G009460 Rw1G013800 Rw1G015100 Rw1G015710 Rw1G016040 Rw1G024780 Rw2G004590 Rw2G026770 Rw2G030550 Rw2G036430 Rw3G011440 Rw3G020600 Rw3G020710 Rw3G025390 Rw3G026320 Rw4G003380 Rw4G016140 Rw4G018350 Rw4G027460 Rw5G004690 Rw5G011140 Rw5G011820 Rw5G016560 Rw5G023230 Rw5G027290 Rw5G028520 Rw5G040540 Rw5G046050 Rw6G013650 Rw6G018020 Rw6G019800 Rw6G023450 Rw7G027610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 395
Acc65I GGTACC 1 cut(s) 113
AccB1I GGYRCC 2 cut(s) 113, 404
AciI CCGC 2 cut(s) 345, 653
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 1 cut(s) 368
AcuI CTGAAG 2 cut(s) 104, 629
AfaI GTAC 2 cut(s) 115, 359
AfiI CCNNNNNNNGG 3 cut(s) 124, 163, 350
AflIII ACRYGT 2 cut(s) 289, 317
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 4 cut(s) 152, 331, 440, 472
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 4 cut(s) 264, 505, 564, 699
AluI AGCT 4 cut(s) 264, 505, 564, 699
Alw26I GTCTC 3 cut(s) 122, 186, 378
AoxI GGCC 2 cut(s) 33, 297
ApeKI GCWGC 2 cut(s) 564, 592
ApoI RAATTY 1 cut(s) 368
AsiGI ACCGGT 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 113
AspLEI GCGC 1 cut(s) 396
AsuHPI GGTGA 2 cut(s) 113, 531
AsuII TTCGAA 1 cut(s) 529
BanI GGYRCC 2 cut(s) 113, 404
BbsI GAAGAC 1 cut(s) 635
BbvI GCAGC 2 cut(s) 551, 579
BccI CCATC 5 cut(s) 80, 416, 426, 543, 667
BciT130I CCWGG 1 cut(s) 202
BcoDI GTCTC 3 cut(s) 122, 186, 378
BfaI CTAG 2 cut(s) 272, 700
BfmI CTRYAG 1 cut(s) 514
BisI GCNGC 2 cut(s) 565, 593
BlsI GCNGC 2 cut(s) 566, 594
Bme1390I CCNGG 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 115, 406
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 2 cut(s) 46, 579
BpiI GAAGAC 1 cut(s) 635
BpmI CTGGAG 1 cut(s) 397
Bpu14I TTCGAA 1 cut(s) 529
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 163, 350
Bse118I RCCGGY 2 cut(s) 98, 575
Bse3DI GCAATG 1 cut(s) 115
BseBI CCWGG 1 cut(s) 202
BseGI GGATG 4 cut(s) 554, 594, 659, 681
BseLI CCNNNNNNNGG 3 cut(s) 124, 163, 350
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 149
BseRI GAGGAG 1 cut(s) 620
BseXI GCAGC 2 cut(s) 551, 579
BsgI GTGCAG 1 cut(s) 578
BshFI GGCC 2 cut(s) 35, 299
BshNI GGYRCC 2 cut(s) 113, 404
BshTI ACCGGT 1 cut(s) 98
BsiSI CCGG 2 cut(s) 99, 576
BslI CCNNNNNNNGG 3 cut(s) 124, 163, 350
BsmAI GTCTC 3 cut(s) 122, 186, 378
BsmI GAATGC 2 cut(s) 59, 478
BsnI GGCC 2 cut(s) 35, 299
Bsp119I TTCGAA 1 cut(s) 529
Bsp143I GATC 1 cut(s) 230
BspACI CCGC 2 cut(s) 345, 653
BspANI GGCC 2 cut(s) 35, 299
BspCNI CTCAG 1 cut(s) 150
BspLI GGNNCC 2 cut(s) 115, 406
BspMAI CTGCAG 1 cut(s) 518
BspT104I TTCGAA 1 cut(s) 529
BspT107I GGYRCC 2 cut(s) 113, 404
BsrDI GCAATG 1 cut(s) 115
BsrFI RCCGGY 2 cut(s) 98, 575
BssAI RCCGGY 2 cut(s) 98, 575
BssMI GATC 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 3 cut(s) 148, 223, 460
Bst6I CTCTTC 1 cut(s) 687
BstBI TTCGAA 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 1 cut(s) 158
BstF5I GGATG 4 cut(s) 554, 594, 659, 681
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 1 cut(s) 233
BstMAI GTCTC 3 cut(s) 122, 186, 378
BstMBI GATC 1 cut(s) 230
BstNI CCWGG 1 cut(s) 202
BstNSI RCATGY 3 cut(s) 293, 321, 364
BstSCI CCNGG 1 cut(s) 200
BstSFI CTRYAG 1 cut(s) 514
BstV1I GCAGC 2 cut(s) 551, 579
BstV2I GAAGAC 1 cut(s) 635
BstXI CCANNNNNNTGG 1 cut(s) 549
BsuRI GGCC 2 cut(s) 35, 299
BtgZI GCGATG 1 cut(s) 181
BtsCI GGATG 4 cut(s) 554, 594, 659, 681
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 2 cut(s) 144, 523
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 1 cut(s) 396
Cfr10I RCCGGY 2 cut(s) 98, 575
Csp6I GTAC 2 cut(s) 114, 358
CspAI ACCGGT 1 cut(s) 98
CviAII CATG 4 cut(s) 290, 318, 361, 496
CviJI RGCY 8 cut(s) 35, 264, 299, 417, 505, 564, 579, 699
CviKI_1 RGCY 8 cut(s) 35, 264, 299, 417, 505, 564, 579, 699
CviQI GTAC 2 cut(s) 114, 358
DdeI CTNAG 1 cut(s) 158
DpnI GATC 1 cut(s) 232
DpnII GATC 1 cut(s) 230
DraI TTTAAA 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 687
EarI CTCTTC 1 cut(s) 687
Eco57I CTGAAG 2 cut(s) 104, 629
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 200
EcoT22I ATGCAT 1 cut(s) 61
FaeI CATG 4 cut(s) 293, 321, 364, 499
FalI AAGNNNNNCTT 2 cut(s) 220, 252
FatI CATG 4 cut(s) 289, 317, 360, 495
FauI CCCGC 2 cut(s) 352, 660
Fnu4HI GCNGC 2 cut(s) 565, 593
FokI GGATG 4 cut(s) 561, 601, 646, 688
Fsp4HI GCNGC 2 cut(s) 565, 593
FspAI RTGCGCAY 1 cut(s) 395
FspBI CTAG 2 cut(s) 272, 700
FspI TGCGCA 1 cut(s) 395
GlaI GCGC 1 cut(s) 395
GluI GCNGC 2 cut(s) 565, 593
GsuI CTGGAG 1 cut(s) 397
HaeIII GGCC 2 cut(s) 35, 299
HapII CCGG 2 cut(s) 99, 576
HhaI GCGC 1 cut(s) 396
Hin1II CATG 4 cut(s) 293, 321, 364, 499
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HinfI GANTC 3 cut(s) 29, 526, 534
HpaII CCGG 2 cut(s) 99, 576
HphI GGTGA 2 cut(s) 113, 531
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 2 cut(s) 466, 648
Hpy188III TCNNGA 1 cut(s) 673
Hpy8I GTNNAC 1 cut(s) 258
HpyAV CCTTC 4 cut(s) 69, 128, 156, 604
HpyCH4III ACNGT 3 cut(s) 148, 223, 460
HpyCH4V TGCA 6 cut(s) 59, 226, 476, 516, 595, 637
HpyF3I CTNAG 1 cut(s) 158
Hsp92II CATG 4 cut(s) 293, 321, 364, 499
HspAI GCGC 1 cut(s) 394
KpnI GGTACC 1 cut(s) 117
Kzo9I GATC 1 cut(s) 230
Lsp1109I GCAGC 2 cut(s) 551, 579
LweI GCATC 2 cut(s) 46, 579
MaeI CTAG 2 cut(s) 272, 700
MaeIII GTNAC 2 cut(s) 454, 581
MalI GATC 1 cut(s) 232
MboI GATC 1 cut(s) 230
MboII GAAGA 4 cut(s) 228, 319, 543, 635
MfeI CAATTG 1 cut(s) 681
MluCI AATT 2 cut(s) 368, 681
MlyI GAGTC 1 cut(s) 38
MmeI TCCRAC 2 cut(s) 74, 444
MnlI CCTC 5 cut(s) 182, 310, 344, 598, 679
Mph1103I ATGCAT 1 cut(s) 61
MseI TTAA 2 cut(s) 14, 480
MslI CAYNNNNRTG 3 cut(s) 15, 318, 600
MspI CCGG 2 cut(s) 99, 576
MspR9I CCNGG 1 cut(s) 202
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 2 cut(s) 59, 478
MvaI CCWGG 1 cut(s) 202
NdeII GATC 1 cut(s) 230
NlaIII CATG 4 cut(s) 293, 321, 364, 499
NlaIV GGNNCC 2 cut(s) 115, 406
NmuCI GTSAC 1 cut(s) 454
NsbI TGCGCA 1 cut(s) 395
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 3 cut(s) 293, 321, 364
NspV TTCGAA 1 cut(s) 529
PciI ACATGT 2 cut(s) 289, 317
PctI GAATGC 2 cut(s) 59, 478
PfeI GAWTC 2 cut(s) 526, 534
PflFI GACNNNGTC 1 cut(s) 460
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 2 cut(s) 566, 594
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
PscI ACATGT 2 cut(s) 289, 317
Psp6I CCWGG 1 cut(s) 200
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 115, 406
PstI CTGCAG 1 cut(s) 518
PsyI GACNNNGTC 1 cut(s) 460
RsaI GTAC 2 cut(s) 115, 359
RsaNI GTAC 2 cut(s) 114, 358
RseI CAYNNNNRTG 3 cut(s) 15, 318, 600
SaqAI TTAA 2 cut(s) 14, 480
SatI GCNGC 2 cut(s) 565, 593
Sau3AI GATC 1 cut(s) 230
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 1 cut(s) 202
SetI ASST 8 cut(s) 126, 159, 206, 266, 393, 507, 566, 701
SfaNI GCATC 2 cut(s) 46, 579
SfcI CTRYAG 1 cut(s) 514
SfuI TTCGAA 1 cut(s) 529
SgrAI CRCCGGYG 1 cut(s) 98
SmiI ATTTAAAT 1 cut(s) 15
SmiMI CAYNNNNRTG 3 cut(s) 15, 318, 600
Sse9I AATT 2 cut(s) 368, 681
SsiI CCGC 2 cut(s) 345, 653
SspI AATATT 1 cut(s) 41
SspMI CTAG 2 cut(s) 272, 700
StyD4I CCNGG 1 cut(s) 200
SwaI ATTTAAAT 1 cut(s) 15
TaaI ACNGT 3 cut(s) 148, 223, 460
TaqI TCGA 3 cut(s) 366, 524, 529
TasI AATT 2 cut(s) 368, 681
TatI WGTACW 1 cut(s) 357
TfiI GAWTC 2 cut(s) 526, 534
Tru1I TTAA 2 cut(s) 14, 480
Tru9I TTAA 2 cut(s) 14, 480
TscAI CASTG 2 cut(s) 151, 523
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 2 cut(s) 564, 592
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 2 cut(s) 36, 300
TspGWI ACGGA 1 cut(s) 621
TspRI CASTG 2 cut(s) 151, 523
Tth111I GACNNNGTC 1 cut(s) 460
XapI RAATTY 1 cut(s) 368
XceI RCATGY 3 cut(s) 293, 321, 364
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XspI CTAG 2 cut(s) 272, 700
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.