Belongs to the helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1140 |
| AciI | CCGC | 2 cut(s) | 410, 939 |
| AclWI | GGATC | 3 cut(s) | 22, 845, 858 |
| AcsI | RAATTY | 7 cut(s) | 55, 320, 380, 523, 575, 1017, 1220 |
| AcuI | CTGAAG | 2 cut(s) | 1157, 1418 |
| AfaI | GTAC | 5 cut(s) | 462, 728, 929, 1080, 1170 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 957 |
| AjnI | CCWGG | 1 cut(s) | 748 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1095, 1127 |
| AleI | CACNNNNGTG | 1 cut(s) | 687 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 147, 179 |
| AluBI | AGCT | 8 cut(s) | 52, 391, 674, 734, 935, 1073, 1216, 1396 |
| AluI | AGCT | 8 cut(s) | 52, 391, 674, 734, 935, 1073, 1216, 1396 |
| Alw21I | GWGCWC | 2 cut(s) | 474, 1218 |
| Alw26I | GTCTC | 3 cut(s) | 85, 638, 1375 |
| AlwI | GGATC | 3 cut(s) | 22, 845, 858 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 833, 1385 |
| AoxI | GGCC | 2 cut(s) | 394, 746 |
| ApeKI | GCWGC | 1 cut(s) | 1359 |
| ApoI | RAATTY | 7 cut(s) | 55, 320, 380, 523, 575, 1017, 1220 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 591, 904 |
| AspS9I | GGNCC | 2 cut(s) | 394, 948 |
| AsuHPI | GGTGA | 4 cut(s) | 52, 600, 764, 1259 |
| AvaII | GGWCC | 1 cut(s) | 948 |
| BamHI | GGATCC | 1 cut(s) | 850 |
| BanII | GRGCYC | 2 cut(s) | 346, 1218 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 282, 314, 753, 785 |
| BbsI | GAAGAC | 1 cut(s) | 119 |
| Bbv12I | GWGCWC | 2 cut(s) | 474, 1218 |
| BbvI | GCAGC | 1 cut(s) | 1371 |
| BccI | CCATC | 4 cut(s) | 116, 205, 378, 487 |
| BciT130I | CCWGG | 1 cut(s) | 750 |
| BclI | TGATCA | 1 cut(s) | 1195 |
| BcoDI | GTCTC | 3 cut(s) | 85, 638, 1375 |
| BfaI | CTAG | 2 cut(s) | 653, 744 |
| BfmI | CTRYAG | 2 cut(s) | 621, 1074 |
| BfuAI | ACCTGC | 1 cut(s) | 1140 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1008 |
| BisI | GCNGC | 1 cut(s) | 1360 |
| BlsI | GCNGC | 1 cut(s) | 1361 |
| BmcAI | AGTACT | 2 cut(s) | 1080, 1170 |
| Bme1390I | CCNGG | 1 cut(s) | 750 |
| Bme18I | GGWCC | 1 cut(s) | 948 |
| BmgT120I | GGNCC | 2 cut(s) | 394, 948 |
| BmiI | GGNNCC | 4 cut(s) | 345, 498, 852, 949 |
| BmrFI | CCNGG | 1 cut(s) | 750 |
| BmrI | ACTGGG | 1 cut(s) | 452 |
| BmsI | GCATC | 1 cut(s) | 1289 |
| BmuI | ACTGGG | 1 cut(s) | 452 |
| BpiI | GAAGAC | 1 cut(s) | 119 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 535, 567 |
| Bsa29I | ATCGAT | 1 cut(s) | 1283 |
| BsaI | GGTCTC | 1 cut(s) | 85 |
| BsaJI | CCNNGG | 2 cut(s) | 1006, 1028 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 147, 177 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 957 |
| Bse1I | ACTGG | 4 cut(s) | 458, 611, 630, 852 |
| Bse3DI | GCAATG | 2 cut(s) | 885, 908 |
| BseBI | CCWGG | 1 cut(s) | 750 |
| BseCI | ATCGAT | 1 cut(s) | 1283 |
| BseDI | CCNNGG | 2 cut(s) | 1006, 1028 |
| BseGI | GGATG | 1 cut(s) | 251 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 957 |
| BseMI | GCAATG | 2 cut(s) | 885, 908 |
| BseMII | CTCAG | 3 cut(s) | 649, 726, 1482 |
| BseNI | ACTGG | 4 cut(s) | 458, 611, 630, 852 |
| BseRI | GAGGAG | 3 cut(s) | 168, 544, 765 |
| BseXI | GCAGC | 1 cut(s) | 1371 |
| BseYI | CCCAGC | 1 cut(s) | 391 |
| BsgI | GTGCAG | 1 cut(s) | 1146 |
| BshFI | GGCC | 2 cut(s) | 396, 748 |
| BshVI | ATCGAT | 1 cut(s) | 1283 |
| BsiHKAI | GWGCWC | 2 cut(s) | 474, 1218 |
| BslFI | GGGAC | 3 cut(s) | 955, 961, 1010 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 957 |
| BsmAI | GTCTC | 3 cut(s) | 85, 638, 1375 |
| BsmFI | GGGAC | 3 cut(s) | 955, 961, 1010 |
| BsnI | GGCC | 2 cut(s) | 396, 748 |
| Bso31I | GGTCTC | 1 cut(s) | 85 |
| Bsp1286I | GDGCHC | 3 cut(s) | 346, 474, 1218 |
| Bsp143I | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| Bsp19I | CCATGG | 2 cut(s) | 1006, 1028 |
| BspACI | CCGC | 2 cut(s) | 410, 939 |
| BspANI | GGCC | 2 cut(s) | 396, 748 |
| BspCNI | CTCAG | 3 cut(s) | 648, 727, 1483 |
| BspDI | ATCGAT | 1 cut(s) | 1283 |
| BspHI | TCATGA | 1 cut(s) | 286 |
| BspLI | GGNNCC | 4 cut(s) | 345, 498, 852, 949 |
| BspMI | ACCTGC | 1 cut(s) | 1140 |
| BspPI | GGATC | 3 cut(s) | 22, 845, 858 |
| BspQI | GCTCTTC | 1 cut(s) | 1401 |
| BspTNI | GGTCTC | 1 cut(s) | 85 |
| BsrDI | GCAATG | 2 cut(s) | 885, 908 |
| BsrI | ACTGG | 4 cut(s) | 458, 611, 630, 852 |
| BssECI | CCNNGG | 2 cut(s) | 1006, 1028 |
| BssMI | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| BssT1I | CCWWGG | 2 cut(s) | 1006, 1028 |
| Bst2UI | CCWGG | 1 cut(s) | 750 |
| Bst4CI | ACNGT | 6 cut(s) | 917, 1078, 1149, 1369, 1432, 1473 |
| Bst6I | CTCTTC | 3 cut(s) | 1334, 1394, 1401 |
| BstC8I | GCNNGC | 1 cut(s) | 937 |
| BstDEI | CTNAG | 6 cut(s) | 38, 171, 583, 635, 735, 1491 |
| BstDSI | CCRYGG | 2 cut(s) | 1006, 1028 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 955 |
| BstF5I | GGATG | 1 cut(s) | 251 |
| BstKTI | GATC | 7 cut(s) | 30, 838, 853, 1060, 1198, 1321, 1416 |
| BstMAI | GTCTC | 3 cut(s) | 85, 638, 1375 |
| BstMBI | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1008, 1417 |
| BstNI | CCWGG | 1 cut(s) | 750 |
| BstSCI | CCNGG | 1 cut(s) | 748 |
| BstSFI | CTRYAG | 2 cut(s) | 621, 1074 |
| BstV1I | GCAGC | 1 cut(s) | 1371 |
| BstV2I | GAAGAC | 1 cut(s) | 119 |
| BstX2I | RGATCY | 1 cut(s) | 850 |
| BstYI | RGATCY | 1 cut(s) | 850 |
| Bsu15I | ATCGAT | 1 cut(s) | 1283 |
| BsuRI | GGCC | 2 cut(s) | 396, 748 |
| BsuTUI | ATCGAT | 1 cut(s) | 1283 |
| BtgI | CCRYGG | 2 cut(s) | 1006, 1028 |
| BtgZI | GCGATG | 1 cut(s) | 576 |
| BtsCI | GGATG | 1 cut(s) | 251 |
| BtsIMutI | CAGTG | 4 cut(s) | 23, 834, 845, 922 |
| BveI | ACCTGC | 1 cut(s) | 1140 |
| Cac8I | GCNNGC | 1 cut(s) | 937 |
| CaiI | CAGNNNCTG | 2 cut(s) | 833, 1385 |
| CciI | TCATGA | 1 cut(s) | 286 |
| Cfr13I | GGNCC | 2 cut(s) | 394, 948 |
| ClaI | ATCGAT | 1 cut(s) | 1283 |
| CseI | GACGC | 1 cut(s) | 588 |
| Csp6I | GTAC | 5 cut(s) | 461, 727, 928, 1079, 1169 |
| CviAII | CATG | 9 cut(s) | 32, 64, 167, 197, 287, 338, 1007, 1029, 1045 |
| CviQI | GTAC | 5 cut(s) | 461, 727, 928, 1079, 1169 |
| DdeI | CTNAG | 6 cut(s) | 38, 171, 583, 635, 735, 1491 |
| DpnI | GATC | 7 cut(s) | 29, 837, 852, 1059, 1197, 1320, 1415 |
| DpnII | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| Eam1104I | CTCTTC | 3 cut(s) | 1334, 1394, 1401 |
| EarI | CTCTTC | 3 cut(s) | 1334, 1394, 1401 |
| Ecl136II | GAGCTC | 1 cut(s) | 1216 |
| Eco130I | CCWWGG | 2 cut(s) | 1006, 1028 |
| Eco24I | GRGCYC | 2 cut(s) | 346, 1218 |
| Eco31I | GGTCTC | 1 cut(s) | 85 |
| Eco47I | GGWCC | 1 cut(s) | 948 |
| Eco53kI | GAGCTC | 1 cut(s) | 1216 |
| Eco57I | CTGAAG | 2 cut(s) | 1157, 1418 |
| EcoICRI | GAGCTC | 1 cut(s) | 1216 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 955 |
| EcoO109I | RGGNCCY | 1 cut(s) | 948 |
| EcoRI | GAATTC | 1 cut(s) | 380 |
| EcoRII | CCWGG | 1 cut(s) | 748 |
| EcoT14I | CCWWGG | 2 cut(s) | 1006, 1028 |
| EcoT38I | GRGCYC | 2 cut(s) | 346, 1218 |
| ErhI | CCWWGG | 2 cut(s) | 1006, 1028 |
| FaeI | CATG | 9 cut(s) | 35, 67, 170, 200, 290, 341, 1010, 1032, 1048 |
| FaqI | GGGAC | 3 cut(s) | 955, 961, 1010 |
| FatI | CATG | 9 cut(s) | 31, 63, 166, 196, 286, 337, 1006, 1028, 1044 |
| FauI | CCCGC | 1 cut(s) | 932 |
| FauNDI | CATATG | 1 cut(s) | 118 |
| FbaI | TGATCA | 1 cut(s) | 1195 |
| Fnu4HI | GCNGC | 1 cut(s) | 1360 |
| FokI | GGATG | 1 cut(s) | 238 |
| FriOI | GRGCYC | 2 cut(s) | 346, 1218 |
| Fsp4HI | GCNGC | 1 cut(s) | 1360 |
| FspBI | CTAG | 2 cut(s) | 653, 744 |
| GluI | GCNGC | 1 cut(s) | 1360 |
| GsaI | CCCAGC | 1 cut(s) | 395 |
| HaeIII | GGCC | 2 cut(s) | 396, 748 |
| HgaI | GACGC | 1 cut(s) | 588 |
| Hin1II | CATG | 9 cut(s) | 35, 67, 170, 200, 290, 341, 1010, 1032, 1048 |
| HincII | GTYRAC | 1 cut(s) | 315 |
| HindII | GTYRAC | 1 cut(s) | 315 |
| HindIII | AAGCTT | 1 cut(s) | 933 |
| HinfI | GANTC | 8 cut(s) | 102, 182, 225, 259, 546, 709, 857, 1382 |
| HphI | GGTGA | 4 cut(s) | 52, 600, 764, 1259 |
| Hpy166II | GTNNAC | 2 cut(s) | 315, 1042 |
| Hpy188I | TCNGA | 6 cut(s) | 187, 638, 835, 899, 1381, 1387 |
| Hpy188III | TCNNGA | 4 cut(s) | 287, 356, 704, 854 |
| Hpy8I | GTNNAC | 2 cut(s) | 315, 1042 |
| Hpy99I | CGWCG | 1 cut(s) | 604 |
| HpyAV | CCTTC | 3 cut(s) | 376, 894, 1135 |
| HpyCH4III | ACNGT | 6 cut(s) | 917, 1078, 1149, 1369, 1432, 1473 |
| HpyCH4IV | ACGT | 1 cut(s) | 301 |
| HpyCH4V | TGCA | 7 cut(s) | 295, 841, 885, 913, 1127, 1183, 1280 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1008, 1417 |
| HpyF3I | CTNAG | 6 cut(s) | 38, 171, 583, 635, 735, 1491 |
| HpySE526I | ACGT | 1 cut(s) | 301 |
| Hsp92II | CATG | 9 cut(s) | 35, 67, 170, 200, 290, 341, 1010, 1032, 1048 |
| Ksp22I | TGATCA | 1 cut(s) | 1195 |
| Kzo9I | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| LguI | GCTCTTC | 1 cut(s) | 1401 |
| LmnI | GCTCC | 2 cut(s) | 349, 1356 |
| Lsp1109I | GCAGC | 1 cut(s) | 1371 |
| LweI | GCATC | 1 cut(s) | 1289 |
| MaeI | CTAG | 2 cut(s) | 653, 744 |
| MaeII | ACGT | 1 cut(s) | 301 |
| MaeIII | GTNAC | 1 cut(s) | 625 |
| MalI | GATC | 7 cut(s) | 29, 837, 852, 1059, 1197, 1320, 1415 |
| MboI | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| MflI | RGATCY | 1 cut(s) | 850 |
| MhlI | GDGCHC | 3 cut(s) | 346, 474, 1218 |
| MroXI | GAANNNNTTC | 2 cut(s) | 591, 904 |
| MseI | TTAA | 3 cut(s) | 140, 783, 993 |
| MslI | CAYNNNNRTG | 1 cut(s) | 687 |
| MspA1I | CMGCKG | 2 cut(s) | 52, 391 |
| MspR9I | CCNGG | 1 cut(s) | 750 |
| MvaI | CCWGG | 1 cut(s) | 750 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1008, 1417 |
| NcoI | CCATGG | 2 cut(s) | 1006, 1028 |
| NdeI | CATATG | 1 cut(s) | 118 |
| NdeII | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| NlaIII | CATG | 9 cut(s) | 35, 67, 170, 200, 290, 341, 1010, 1032, 1048 |
| NlaIV | GGNNCC | 4 cut(s) | 345, 498, 852, 949 |
| NmuCI | GTSAC | 1 cut(s) | 625 |
| OliI | CACNNNNGTG | 1 cut(s) | 687 |
| PagI | TCATGA | 1 cut(s) | 286 |
| PciSI | GCTCTTC | 1 cut(s) | 1401 |
| PdmI | GAANNNNTTC | 2 cut(s) | 591, 904 |
| PfeI | GAWTC | 8 cut(s) | 102, 182, 225, 259, 546, 709, 857, 1382 |
| PkrI | GCNGC | 1 cut(s) | 1361 |
| PpuMI | RGGWCCY | 1 cut(s) | 948 |
| Psp124BI | GAGCTC | 1 cut(s) | 1218 |
| Psp5II | RGGWCCY | 1 cut(s) | 948 |
| Psp6I | CCWGG | 1 cut(s) | 748 |
| PspFI | CCCAGC | 1 cut(s) | 391 |
| PspGI | CCWGG | 1 cut(s) | 748 |
| PspN4I | GGNNCC | 4 cut(s) | 345, 498, 852, 949 |
| PspPI | GGNCC | 2 cut(s) | 394, 948 |
| PspPPI | RGGWCCY | 1 cut(s) | 948 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1152, 1184 |
| PstNI | CAGNNNCTG | 2 cut(s) | 833, 1385 |
| PsuI | RGATCY | 1 cut(s) | 850 |
| PvuII | CAGCTG | 2 cut(s) | 52, 391 |
| RsaI | GTAC | 5 cut(s) | 462, 728, 929, 1080, 1170 |
| RsaNI | GTAC | 5 cut(s) | 461, 727, 928, 1079, 1169 |
| RseI | CAYNNNNRTG | 1 cut(s) | 687 |
| SacI | GAGCTC | 1 cut(s) | 1218 |
| SapI | GCTCTTC | 1 cut(s) | 1401 |
| SaqAI | TTAA | 3 cut(s) | 140, 783, 993 |
| SatI | GCNGC | 1 cut(s) | 1360 |
| Sau3AI | GATC | 7 cut(s) | 27, 835, 850, 1057, 1195, 1318, 1413 |
| Sau96I | GGNCC | 2 cut(s) | 394, 948 |
| ScaI | AGTACT | 2 cut(s) | 1080, 1170 |
| ScrFI | CCNGG | 1 cut(s) | 750 |
| SduI | GDGCHC | 3 cut(s) | 346, 474, 1218 |
| SfaNI | GCATC | 1 cut(s) | 1289 |
| SfcI | CTRYAG | 2 cut(s) | 621, 1074 |
| SinI | GGWCC | 1 cut(s) | 948 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 687 |
| SsiI | CCGC | 2 cut(s) | 410, 939 |
| SspI | AATATT | 1 cut(s) | 362 |
| SspMI | CTAG | 2 cut(s) | 653, 744 |
| SstI | GAGCTC | 1 cut(s) | 1218 |
| StyD4I | CCNGG | 1 cut(s) | 748 |
| StyI | CCWWGG | 2 cut(s) | 1006, 1028 |
| TaaI | ACNGT | 6 cut(s) | 917, 1078, 1149, 1369, 1432, 1473 |
| TaiI | ACGT | 1 cut(s) | 304 |
| TaqI | TCGA | 4 cut(s) | 579, 595, 1173, 1283 |
| TatI | WGTACW | 4 cut(s) | 460, 726, 1078, 1168 |
| TfiI | GAWTC | 8 cut(s) | 102, 182, 225, 259, 546, 709, 857, 1382 |
| Tru1I | TTAA | 3 cut(s) | 140, 783, 993 |
| Tru9I | TTAA | 3 cut(s) | 140, 783, 993 |
| TscAI | CASTG | 4 cut(s) | 30, 834, 852, 922 |
| TseFI | GTSAC | 1 cut(s) | 625 |
| TseI | GCWGC | 1 cut(s) | 1359 |
| Tsp45I | GTSAC | 1 cut(s) | 625 |
| TspDTI | ATGAA | 9 cut(s) | 48, 155, 162, 303, 685, 849, 897, 1244, 1491 |
| TspRI | CASTG | 4 cut(s) | 30, 834, 852, 922 |
| VpaK11BI | GGWCC | 1 cut(s) | 948 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 955 |
| XapI | RAATTY | 7 cut(s) | 55, 320, 380, 523, 575, 1017, 1220 |
| XmnI | GAANNNNTTC | 2 cut(s) | 591, 904 |
| XspI | CTAG | 2 cut(s) | 653, 744 |
| ZrmI | AGTACT | 2 cut(s) | 1080, 1170 |