Rorug03G0283000

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002539
Physical Location & Seq
Reverse (-)
28425909 .. 28428995
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0283000.1

Sequence Viewer

Length: 843 bp
ATGTCCTTGAAATCAATCTGCTTCTCTGTTTGGCTCCCTAGCCCTTCTCCGAGTACCGCTGCTCCTGCTTCAATCTCTTCCCCCTATTCCTTTCCTTTGAGAAATCCAAACCACTCCGTCTCCAATTCCGGTTCTTCAAGGCCTCACCTGCTGCATTTCTCGAACAAGCTCGACTCCGCGTTCTTGTCTGAGGATGACGACGATTCCGATTCCGATTCCGATTCAGAAGCAGAGAAATCGGAAGTTGATATAGATGTAGGTGTAGAGTTAGAGATAGAGAAGACGGGGTCCAACTGCCGGAGAATCCGATCGGAAATCGGAATAGAGGCGCCGCTCGCCACCGTCTGGAATTTGTTGACGGATTACGAGAGACTGGCGGACTTCATTCCCGGACTCGCCGTCTGCCAGTTGCTCCAGAAGTCGGAGAACTACGCTCGGCTTTTTCAGATTGGAGAGCAGGACTTGGCGTTTGGGCTGAAATTCAACGCGAAAGGAGTGGTGGATTGTTATGAGAATCCTGTAGAGAGCATTGCTAATGATTTGGGGGGTCTCACTCTGAAGCGTGATATTGAGTTTAACATGGTTGAAGGTGACTTTGAAATCTTTCGAGGCAAGTGGTCTCTCCAACAGCTTAACACTAGTGGGGAAATAGCATGTGATCCTTTGATAGACCAGAAAATGCAAACAACAACTACTCTTTCCTACTTGGTTGATGTAAAGCCGAAGATGTTGTTGCCGGTTCAGCTGGTTGAGGGTAGACTCTGCAAGGAGATCAAAATAAACCTAGCTTGCATCCGGGAAGAAGCACTGAAAATGACTCGCGAAACTCTTCTTACTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

280

Amino Acids

31.14

Weight (kDa)

4.69

Isoelectric Point (pI)

55.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc PF03364 108 - 256 3.6e-19 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 156
Acc36I ACCTGC 1 cut(s) 156
AccB1I GGYRCC 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 345
AccBSI CCGCTC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 757
AccII CGCG 3 cut(s) 179, 488, 822
AciI CCGC 4 cut(s) 57, 177, 332, 377
AclWI GGATC 1 cut(s) 653
AcsI RAATTY 2 cut(s) 349, 479
AcuI CTGAAG 1 cut(s) 578
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 3 cut(s) 297, 345, 421
AgsI TTSAA 6 cut(s) 10, 72, 138, 484, 587, 599
AhdI GACNNNNNGTC 1 cut(s) 398
AhlI ACTAGT 1 cut(s) 638
AluBI AGCT 4 cut(s) 169, 631, 745, 788
AluI AGCT 4 cut(s) 169, 631, 745, 788
Alw26I GTCTC 4 cut(s) 124, 364, 554, 624
AlwI GGATC 1 cut(s) 653
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 2 cut(s) 59, 151
ApoI RAATTY 2 cut(s) 349, 479
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
Asp700I GAANNNNTTC 2 cut(s) 603, 828
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 288
AsuC2I CCSGG 2 cut(s) 390, 797
AsuHPI GGTGA 2 cut(s) 137, 602
AvaII GGWCC 1 cut(s) 288
BanI GGYRCC 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 46, 138
BceAI ACGGC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 219, 253
BcnI CCSGG 2 cut(s) 390, 797
BcoDI GTCTC 4 cut(s) 124, 364, 554, 624
BcuI ACTAGT 1 cut(s) 638
BfaI CTAG 3 cut(s) 39, 639, 785
BfmI CTRYAG 1 cut(s) 519
BfoI RGCGCY 1 cut(s) 332
BfuAI ACCTGC 1 cut(s) 156
BisI GCNGC 3 cut(s) 60, 152, 332
BlsI GCNGC 3 cut(s) 61, 153, 333
Bme1390I CCNGG 2 cut(s) 390, 797
Bme18I GGWCC 1 cut(s) 288
BmeRI GACNNNNNGTC 1 cut(s) 398
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 3 cut(s) 35, 289, 330
BmrFI CCNGG 2 cut(s) 390, 797
BmsI GCATC 1 cut(s) 801
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 398
BpuMI CCSGG 2 cut(s) 390, 797
BsaHI GRCGYC 1 cut(s) 329
BsaI GGTCTC 2 cut(s) 554, 624
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 297, 345, 421
Bse118I RCCGGY 1 cut(s) 736
Bse1I ACTGG 2 cut(s) 378, 406
Bse3DI GCAATG 1 cut(s) 528
BseGI GGATG 2 cut(s) 199, 792
BseLI CCNNNNNNNGG 3 cut(s) 297, 345, 421
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 378, 406
BseXI GCAGC 2 cut(s) 46, 138
Bsh1236I CGCG 3 cut(s) 179, 488, 822
Bsh1285I CGRYCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 328
BsiEI CGRYCG 1 cut(s) 311
BsiSI CCGG 5 cut(s) 129, 298, 390, 737, 796
BslI CCNNNNNNNGG 3 cut(s) 297, 345, 421
BsmAI GTCTC 4 cut(s) 124, 364, 554, 624
BsmBI CGTCTC 1 cut(s) 124
BsnI GGCC 1 cut(s) 142
Bso31I GGTCTC 2 cut(s) 554, 624
Bsp143I GATC 3 cut(s) 308, 658, 771
Bsp68I TCGCGA 1 cut(s) 822
BspACI CCGC 4 cut(s) 57, 177, 332, 377
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 3 cut(s) 179, 488, 822
BspLI GGNNCC 3 cut(s) 35, 289, 330
BspMI ACCTGC 1 cut(s) 156
BspPI GGATC 1 cut(s) 653
BspT107I GGYRCC 1 cut(s) 328
BspTNI GGTCTC 2 cut(s) 554, 624
BsrBI CCGCTC 1 cut(s) 334
BsrDI GCAATG 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 736
BsrI ACTGG 2 cut(s) 378, 406
BssAI RCCGGY 1 cut(s) 736
BssMI GATC 3 cut(s) 308, 658, 771
BssNI GRCGYC 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 343
Bst6I CTCTTC 2 cut(s) 82, 834
BstACI GRCGYC 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 336, 790
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 2 cut(s) 199, 792
BstFNI CGCG 3 cut(s) 179, 488, 822
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 3 cut(s) 311, 661, 774
BstMAI GTCTC 4 cut(s) 124, 364, 554, 624
BstMBI GATC 3 cut(s) 308, 658, 771
BstMCI CGRYCG 1 cut(s) 311
BstMWI GCNNNNNNNGC 4 cut(s) 65, 148, 335, 742
BstNSI RCATGY 1 cut(s) 657
BstSCI CCNGG 2 cut(s) 388, 795
BstSFI CTRYAG 1 cut(s) 519
BstUI CGCG 3 cut(s) 179, 488, 822
BstV1I GCAGC 2 cut(s) 46, 138
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 142
BtsCI GGATG 2 cut(s) 199, 792
BtsIMutI CAGTG 1 cut(s) 806
BtuMI TCGCGA 1 cut(s) 822
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 336, 790
CfoI GCGC 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 736
Cfr13I GGNCC 1 cut(s) 288
Csp6I GTAC 1 cut(s) 54
CviAII CATG 2 cut(s) 580, 654
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 1 cut(s) 189
DinI GGCGCC 1 cut(s) 330
DpnI GATC 3 cut(s) 310, 660, 773
DpnII GATC 3 cut(s) 308, 658, 771
DriI GACNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 2 cut(s) 82, 834
Eam1105I GACNNNNNGTC 1 cut(s) 398
EarI CTCTTC 2 cut(s) 82, 834
EciI GGCGGA 1 cut(s) 392
Eco147I AGGCCT 1 cut(s) 142
Eco31I GGTCTC 2 cut(s) 554, 624
Eco47I GGWCC 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 578
EgeI GGCGCC 1 cut(s) 330
EheI GGCGCC 1 cut(s) 330
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 2 cut(s) 583, 657
FaiI YATR 4 cut(s) 251, 510, 581, 655
FatI CATG 2 cut(s) 579, 653
FblI GTMKAC 1 cut(s) 757
Fnu4HI GCNGC 3 cut(s) 60, 152, 332
FokI GGATG 2 cut(s) 206, 779
Fsp4HI GCNGC 3 cut(s) 60, 152, 332
FspBI CTAG 3 cut(s) 39, 639, 785
GlaI GCGC 1 cut(s) 330
GluI GCNGC 3 cut(s) 60, 152, 332
GsuI CTGGAG 1 cut(s) 398
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 1 cut(s) 142
HapII CCGG 5 cut(s) 129, 298, 390, 737, 796
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 2 cut(s) 583, 657
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HpaII CCGG 5 cut(s) 129, 298, 390, 737, 796
HphI GGTGA 2 cut(s) 137, 602
Hpy166II GTNNAC 2 cut(s) 357, 758
Hpy188III TCNNGA 4 cut(s) 160, 346, 415, 821
Hpy8I GTNNAC 2 cut(s) 357, 758
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 2 cut(s) 54, 581
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4V TGCA 4 cut(s) 154, 682, 765, 792
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 148, 335, 742
HpyF3I CTNAG 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 2 cut(s) 583, 657
HspAI GCGC 1 cut(s) 329
KasI GGCGCC 1 cut(s) 328
Kzo9I GATC 3 cut(s) 308, 658, 771
LmnI GCTCC 3 cut(s) 39, 67, 417
Lsp1109I GCAGC 2 cut(s) 46, 138
LweI GCATC 1 cut(s) 801
MaeI CTAG 3 cut(s) 39, 639, 785
MaeIII GTNAC 1 cut(s) 590
MalI GATC 3 cut(s) 310, 660, 773
MbiI CCGCTC 1 cut(s) 334
MboI GATC 3 cut(s) 308, 658, 771
MboII GAAGA 6 cut(s) 69, 126, 292, 736, 812, 821
MluCI AATT 3 cut(s) 124, 349, 479
Mly113I GGCGCC 1 cut(s) 329
MlyI GAGTC 4 cut(s) 167, 387, 753, 811
MmeI TCCRAC 3 cut(s) 315, 402, 649
MnlI CCTC 5 cut(s) 153, 184, 319, 602, 745
MroXI GAANNNNTTC 2 cut(s) 603, 828
MseI TTAA 2 cut(s) 576, 633
MspA1I CMGCKG 2 cut(s) 59, 745
MspI CCGG 5 cut(s) 129, 298, 390, 737, 796
MspR9I CCNGG 2 cut(s) 390, 797
MvnI CGCG 3 cut(s) 179, 488, 822
MwoI GCNNNNNNNGC 4 cut(s) 65, 148, 335, 742
NarI GGCGCC 1 cut(s) 329
NciI CCSGG 2 cut(s) 390, 797
NdeII GATC 3 cut(s) 308, 658, 771
NlaIII CATG 2 cut(s) 583, 657
NlaIV GGNNCC 3 cut(s) 35, 289, 330
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 1 cut(s) 590
NruI TCGCGA 1 cut(s) 822
NspI RCATGY 1 cut(s) 657
PaqCI CACCTGC 1 cut(s) 156
PceI AGGCCT 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PdmI GAANNNNTTC 2 cut(s) 603, 828
PfeI GAWTC 6 cut(s) 203, 209, 215, 221, 303, 514
PflFI GACNNNGTC 1 cut(s) 286
PflMI CCANNNNNTGG 1 cut(s) 345
PfoI TCCNGGA 2 cut(s) 388, 795
PkrI GCNGC 3 cut(s) 61, 153, 333
Ple19I CGATCG 1 cut(s) 311
PleI GAGTC 4 cut(s) 167, 387, 753, 811
PluTI GGCGCC 1 cut(s) 332
PpsI GAGTC 4 cut(s) 167, 387, 753, 811
PspN4I GGNNCC 3 cut(s) 35, 289, 330
PspPI GGNCC 1 cut(s) 288
PsyI GACNNNGTC 1 cut(s) 286
PvuI CGATCG 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 745
RruI TCGCGA 1 cut(s) 822
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SaqAI TTAA 2 cut(s) 576, 633
SatI GCNGC 3 cut(s) 60, 152, 332
Sau3AI GATC 3 cut(s) 308, 658, 771
Sau96I GGNCC 1 cut(s) 288
SchI GAGTC 4 cut(s) 167, 387, 753, 811
ScrFI CCNGG 2 cut(s) 390, 797
SetI ASST 8 cut(s) 150, 171, 262, 592, 633, 747, 786, 790
SfaNI GCATC 1 cut(s) 801
SfcI CTRYAG 1 cut(s) 519
SfoI GGCGCC 1 cut(s) 330
SinI GGWCC 1 cut(s) 288
SpeI ACTAGT 1 cut(s) 638
Sse9I AATT 3 cut(s) 124, 349, 479
SseBI AGGCCT 1 cut(s) 142
SsiI CCGC 4 cut(s) 57, 177, 332, 377
SspDI GGCGCC 1 cut(s) 328
SspMI CTAG 3 cut(s) 39, 639, 785
StuI AGGCCT 1 cut(s) 142
StyD4I CCNGG 2 cut(s) 388, 795
TaaI ACNGT 1 cut(s) 343
TaqI TCGA 3 cut(s) 161, 171, 607
TasI AATT 3 cut(s) 124, 349, 479
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 6 cut(s) 203, 209, 215, 221, 303, 514
Tru1I TTAA 2 cut(s) 576, 633
Tru9I TTAA 2 cut(s) 576, 633
TscAI CASTG 1 cut(s) 813
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 2 cut(s) 59, 151
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 1 cut(s) 373
TspGWI ACGGA 2 cut(s) 106, 374
TspRI CASTG 1 cut(s) 813
Tth111I GACNNNGTC 1 cut(s) 286
Van91I CCANNNNNTGG 1 cut(s) 345
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 2 cut(s) 349, 479
XceI RCATGY 1 cut(s) 657
XmiI GTMKAC 1 cut(s) 757
XmnI GAANNNNTTC 2 cut(s) 603, 828
XspI CTAG 3 cut(s) 39, 639, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.