Rorug04G0105000

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002539
Physical Location & Seq
Reverse (-)
16290451 .. 16291758
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0105000.1

Sequence Viewer

Length: 1065 bp
ATGGCATTGCCATTGAACCCTTGGTTGTCACTTGTGAAATCTTCATTTGTTCAAAACCTTCTCTCCCGCTCCATGTGCCTTGCTTCAATTTCAATAAATTCCAGGATGGTTACCAATTTCCCAGCTTTCCTTGTGCTACTTTTCGTGATCGGTGTTGCTCAAGGACTGCAGGTTGGCTTTTACAGCCAGTCCTGCCCGGCAGCTGAGTCCATCGTAACCGCGGTGGTCAGAGAGGCAGTCCGGACCAATCCTCGGACCGCGCCGATTCTCCTCCGACTGCATTTTCATGACTGCTTTGTGGATGGCTGCGACGGTTCTATTCTACTGGATTCCGGCAACAATCCCGAGAAGATAGCATTCGCACATGCAGGAGTAATGGGATTTGATGTGATCGAGTCGGCGAAATCAAGACTGGAGGCGGTCTGCCAAGGAGTCGTTTCCTGTTCTGATATAGTTGCTCTCGCTGCGCGCGATGCTATTTCATTGGCAAACGGCCCCTTCTATCAGGTCCCGACCGGCCGAAAGGATGGTCGGGTGTCAAACGCCGATCACGCCTCAAGAATGCCGGACGTCGATGAGCCGATCAATGTGCTCAAATCTAAATTTAGCGAAAAGGGACTTGCTGAGAAAGAGCTGGTACTGCTCACAGCTGGAGCTCACACCATGGGAACCACAGCCTGTTTCTTCATGCAAAACAGGCTGTACAACTTCAATTCAACTGCAAAATCGGATCCCTCAATCAACCCGAAGTTTCTGCCGAAACTCAAAAGCATTTGCCCGCTCGGAGGGGACGTGAATGTGAGAACTTCACTTGATCCAATGACGAATACTACATTTGATGACCAGCCGTTCTCTAACCTGAAGGGGGGTTTCGCCGTGCTGGCTTCCGATGCCAGACTGATGGAGGACCCGGTGACCCGAGGGCTGGTCGAGTCGTACCTCAATCTCAAACAAGAAGCCTCCTTTGAAAAAGACTTCGTAGACTCTATTCTGAAGCTGGGGGCCATTGGTGTCAAGTCCGGGGCACAAGGGGAGATTAGGAGGAAATGCAGCGACATGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

354

Amino Acids

38.2

Weight (kDa)

6.94

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 72 - 314 2.1e-75 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 573
Acc36I ACCTGC 1 cut(s) 160
AccBSI CCGCTC 2 cut(s) 69, 781
AccI GTMKAC 1 cut(s) 981
AccII CGCG 4 cut(s) 221, 260, 469, 471
AccIII TCCGGA 1 cut(s) 240
AciI CCGC 6 cut(s) 67, 219, 221, 258, 419, 779
AclWI GGATC 3 cut(s) 725, 738, 809
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 2 cut(s) 97, 602
AcuI CTGAAG 2 cut(s) 881, 1013
AcyI GRCGYC 1 cut(s) 570
AfaI GTAC 3 cut(s) 639, 704, 938
AfiI CCNNNNNNNGG 4 cut(s) 252, 785, 865, 925
AgsI TTSAA 7 cut(s) 16, 53, 87, 93, 712, 717, 968
AjiI CACGTC 1 cut(s) 793
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 6 cut(s) 125, 203, 634, 650, 656, 997
AluI AGCT 6 cut(s) 125, 203, 634, 650, 656, 997
Alw21I GWGCWC 2 cut(s) 594, 658
AlwI GGATC 3 cut(s) 725, 738, 809
Ama87I CYCGRG 2 cut(s) 344, 918
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 3 cut(s) 493, 517, 1002
ApeKI GCWGC 4 cut(s) 200, 306, 464, 1050
ApoI RAATTY 2 cut(s) 97, 602
ArsI GACNNNNNNTTYG 2 cut(s) 514, 546
AspLEI GCGC 3 cut(s) 262, 469, 471
AspS9I GGNCC 6 cut(s) 243, 255, 494, 508, 907, 1002
AsuC2I CCSGG 3 cut(s) 197, 911, 1021
AsuHPI GGTGA 1 cut(s) 925
AvaI CYCGRG 2 cut(s) 344, 918
AvaII GGWCC 4 cut(s) 243, 255, 508, 907
BaeGI GKGCMC 1 cut(s) 1027
BamHI GGATCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 658
Bbv12I GWGCWC 2 cut(s) 594, 658
BbvI GCAGC 3 cut(s) 212, 293, 451
BccI CCATC 5 cut(s) 100, 218, 296, 521, 895
BceAI ACGGC 3 cut(s) 508, 832, 860
BcgI CGANNNNNNTGC 4 cut(s) 571, 605, 736, 770
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 3 cut(s) 197, 911, 1021
BfmI CTRYAG 1 cut(s) 167
BfuAI ACCTGC 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 881
BisI GCNGC 4 cut(s) 201, 307, 465, 1051
BlsI GCNGC 4 cut(s) 202, 308, 466, 1052
Bme1390I CCNGG 4 cut(s) 103, 197, 911, 1021
Bme18I GGWCC 4 cut(s) 243, 255, 508, 907
BmeT110I CYCGRG 2 cut(s) 344, 918
BmgBI CACGTC 1 cut(s) 793
BmgT120I GGNCC 6 cut(s) 243, 255, 494, 508, 907, 1002
BmiI GGNNCC 6 cut(s) 496, 510, 670, 732, 909, 1003
BmrFI CCNGG 4 cut(s) 103, 197, 911, 1021
BmsI GCATC 2 cut(s) 463, 880
BpmI CTGGAG 2 cut(s) 434, 672
BpuEI CTTGAG 2 cut(s) 144, 541
BpuMI CCSGG 3 cut(s) 197, 911, 1021
BsaHI GRCGYC 1 cut(s) 570
BsaJI CCNNGG 7 cut(s) 20, 219, 251, 427, 663, 919, 1020
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 777, 807
Bsc4I CCNNNNNNNGG 4 cut(s) 252, 785, 865, 925
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 3 cut(s) 187, 330, 417
Bse3DI GCAATG 1 cut(s) 5
BseAI TCCGGA 1 cut(s) 240
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 7 cut(s) 20, 219, 251, 427, 663, 919, 1020
BseGI GGATG 3 cut(s) 111, 307, 532
BseLI CCNNNNNNNGG 4 cut(s) 252, 785, 865, 925
BseMI GCAATG 1 cut(s) 5
BseMII CTCAG 2 cut(s) 195, 615
BseNI ACTGG 3 cut(s) 187, 330, 417
BsePI GCGCGC 1 cut(s) 467
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 1027
BseX3I CGGCCG 1 cut(s) 517
BseXI GCAGC 3 cut(s) 212, 293, 451
BseYI CCCAGC 2 cut(s) 121, 997
Bsh1236I CGCG 4 cut(s) 221, 260, 469, 471
Bsh1285I CGRYCG 2 cut(s) 516, 520
BshFI GGCC 3 cut(s) 495, 519, 1004
BsiEI CGRYCG 2 cut(s) 516, 520
BsiHKAI GWGCWC 2 cut(s) 594, 658
BsiHKCI CYCGRG 2 cut(s) 344, 918
BsiSI CCGG 7 cut(s) 197, 241, 333, 516, 566, 911, 1020
BslFI GGGAC 3 cut(s) 494, 630, 803
BslI CCNNNNNNNGG 4 cut(s) 252, 785, 865, 925
BsmFI GGGAC 3 cut(s) 494, 630, 803
BsmI GAATGC 2 cut(s) 356, 567
BsnI GGCC 3 cut(s) 495, 519, 1004
BsoBI CYCGRG 2 cut(s) 344, 918
Bsp1286I GDGCHC 3 cut(s) 594, 658, 1027
Bsp13I TCCGGA 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 6 cut(s) 147, 390, 547, 582, 730, 814
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 6 cut(s) 67, 219, 221, 258, 419, 779
BspANI GGCC 3 cut(s) 495, 519, 1004
BspCNI CTCAG 2 cut(s) 196, 616
BspEI TCCGGA 1 cut(s) 240
BspFNI CGCG 4 cut(s) 221, 260, 469, 471
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 6 cut(s) 496, 510, 670, 732, 909, 1003
BspMAI CTGCAG 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 3 cut(s) 725, 738, 809
BsrBI CCGCTC 2 cut(s) 69, 781
BsrDI GCAATG 1 cut(s) 5
BsrFI RCCGGY 1 cut(s) 515
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 3 cut(s) 187, 330, 417
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 7 cut(s) 20, 219, 251, 427, 663, 919, 1020
BssHII GCGCGC 1 cut(s) 467
BssMI GATC 6 cut(s) 147, 390, 547, 582, 730, 814
BssNI GRCGYC 1 cut(s) 570
BssT1I CCWWGG 3 cut(s) 20, 427, 663
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 314
BstACI GRCGYC 1 cut(s) 570
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 3 cut(s) 469, 779, 882
BstDEI CTNAG 2 cut(s) 204, 624
BstDSI CCRYGG 2 cut(s) 219, 663
BstEII GGTNACC 2 cut(s) 109, 913
BstF5I GGATG 3 cut(s) 111, 307, 532
BstFNI CGCG 4 cut(s) 221, 260, 469, 471
BstHHI GCGC 3 cut(s) 262, 469, 471
BstKTI GATC 6 cut(s) 150, 393, 550, 585, 733, 817
BstMBI GATC 6 cut(s) 147, 390, 547, 582, 730, 814
BstMCI CGRYCG 2 cut(s) 516, 520
BstNI CCWGG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 368
BstPI GGTNACC 2 cut(s) 109, 913
BstSCI CCNGG 4 cut(s) 101, 195, 909, 1019
BstSFI CTRYAG 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 1027
BstUI CGCG 4 cut(s) 221, 260, 469, 471
BstV1I GCAGC 3 cut(s) 212, 293, 451
BstX2I RGATCY 1 cut(s) 730
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 1 cut(s) 730
BstZI CGGCCG 1 cut(s) 517
BsuRI GGCC 3 cut(s) 495, 519, 1004
BtgI CCRYGG 2 cut(s) 219, 663
BtgZI GCGATG 1 cut(s) 486
BtrI CACGTC 1 cut(s) 793
BtsCI GGATG 3 cut(s) 111, 307, 532
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 3 cut(s) 469, 779, 882
CciI TCATGA 1 cut(s) 286
CfoI GCGC 3 cut(s) 262, 469, 471
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 6 cut(s) 243, 255, 494, 508, 907, 1002
Cfr42I CCGCGG 1 cut(s) 222
CpoI CGGWCCG 1 cut(s) 255
Csp6I GTAC 3 cut(s) 638, 703, 937
CspI CGGWCCG 1 cut(s) 255
CviAII CATG 6 cut(s) 73, 287, 365, 664, 688, 1057
CviQI GTAC 3 cut(s) 638, 703, 937
DdeI CTNAG 2 cut(s) 204, 624
DpnI GATC 6 cut(s) 149, 392, 549, 584, 732, 816
DpnII GATC 6 cut(s) 147, 390, 547, 582, 730, 814
EaeI YGGCCR 1 cut(s) 517
EagI CGGCCG 1 cut(s) 517
Ecl136II GAGCTC 1 cut(s) 656
EclXI CGGCCG 1 cut(s) 517
Eco130I CCWWGG 3 cut(s) 20, 427, 663
Eco24I GRGCYC 1 cut(s) 658
Eco47I GGWCC 4 cut(s) 243, 255, 508, 907
Eco52I CGGCCG 1 cut(s) 517
Eco53kI GAGCTC 1 cut(s) 656
Eco57I CTGAAG 2 cut(s) 881, 1013
Eco88I CYCGRG 2 cut(s) 344, 918
Eco91I GGTNACC 2 cut(s) 109, 913
EcoICRI GAGCTC 1 cut(s) 656
EcoO109I RGGNCCY 2 cut(s) 508, 907
EcoO65I GGTNACC 2 cut(s) 109, 913
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 3 cut(s) 20, 427, 663
EcoT38I GRGCYC 1 cut(s) 658
ErhI CCWWGG 3 cut(s) 20, 427, 663
FaeI CATG 6 cut(s) 76, 290, 368, 667, 691, 1060
FaiI YATR 7 cut(s) 74, 288, 366, 452, 665, 689, 1058
FaqI GGGAC 3 cut(s) 494, 630, 803
FatI CATG 6 cut(s) 72, 286, 364, 663, 687, 1056
FauI CCCGC 2 cut(s) 74, 786
FblI GTMKAC 1 cut(s) 981
Fnu4HI GCNGC 4 cut(s) 201, 307, 465, 1051
FokI GGATG 3 cut(s) 118, 314, 539
FriOI GRGCYC 1 cut(s) 658
Fsp4HI GCNGC 4 cut(s) 201, 307, 465, 1051
GlaI GCGC 3 cut(s) 261, 468, 470
GluI GCNGC 4 cut(s) 201, 307, 465, 1051
GsaI CCCAGC 2 cut(s) 125, 1001
GsuI CTGGAG 2 cut(s) 434, 672
HaeIII GGCC 3 cut(s) 495, 519, 1004
HapII CCGG 7 cut(s) 197, 241, 333, 516, 566, 911, 1020
HhaI GCGC 3 cut(s) 262, 469, 471
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 6 cut(s) 76, 290, 368, 667, 691, 1060
Hin6I GCGC 3 cut(s) 260, 467, 469
HinP1I GCGC 3 cut(s) 260, 467, 469
HinfI GANTC 7 cut(s) 206, 265, 329, 395, 432, 932, 983
HpaII CCGG 7 cut(s) 197, 241, 333, 516, 566, 911, 1020
HphI GGTGA 1 cut(s) 925
Hpy166II GTNNAC 1 cut(s) 982
Hpy188I TCNGA 8 cut(s) 230, 255, 275, 448, 730, 785, 889, 993
Hpy188III TCNNGA 7 cut(s) 145, 241, 287, 344, 408, 511, 558
Hpy8I GTNNAC 1 cut(s) 982
Hpy99I CGWCG 2 cut(s) 314, 575
HpyAV CCTTC 3 cut(s) 68, 508, 856
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 2 cut(s) 570, 792
HpyCH4V TGCA 6 cut(s) 169, 280, 368, 691, 722, 1050
HpyF3I CTNAG 2 cut(s) 204, 624
HpySE526I ACGT 2 cut(s) 570, 792
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 6 cut(s) 76, 290, 368, 667, 691, 1060
HspAI GCGC 3 cut(s) 260, 467, 469
Kpn2I TCCGGA 1 cut(s) 240
KspI CCGCGG 1 cut(s) 222
Kzo9I GATC 6 cut(s) 147, 390, 547, 582, 730, 814
LmnI GCTCC 2 cut(s) 74, 653
Lsp1109I GCAGC 3 cut(s) 212, 293, 451
LweI GCATC 2 cut(s) 463, 880
MaeII ACGT 2 cut(s) 570, 792
MaeIII GTNAC 4 cut(s) 27, 109, 214, 913
MalI GATC 6 cut(s) 149, 392, 549, 584, 732, 816
MbiI CCGCTC 2 cut(s) 69, 781
MboI GATC 6 cut(s) 147, 390, 547, 582, 730, 814
MboII GAAGA 3 cut(s) 33, 361, 676
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 3 cut(s) 594, 658, 1027
MluCI AATT 5 cut(s) 87, 97, 115, 602, 712
MlyI GAGTC 5 cut(s) 215, 404, 441, 941, 977
MmeI TCCRAC 1 cut(s) 298
MroI TCCGGA 1 cut(s) 240
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 3 cut(s) 203, 221, 650
MspI CCGG 7 cut(s) 197, 241, 333, 516, 566, 911, 1020
MspR9I CCNGG 4 cut(s) 103, 197, 911, 1021
Mva1269I GAATGC 2 cut(s) 356, 567
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 4 cut(s) 221, 260, 469, 471
NciI CCSGG 3 cut(s) 197, 911, 1021
NcoI CCATGG 1 cut(s) 663
NdeII GATC 6 cut(s) 147, 390, 547, 582, 730, 814
NlaIII CATG 6 cut(s) 76, 290, 368, 667, 691, 1060
NlaIV GGNNCC 6 cut(s) 496, 510, 670, 732, 909, 1003
NmuCI GTSAC 2 cut(s) 27, 913
NspI RCATGY 1 cut(s) 368
PagI TCATGA 1 cut(s) 286
PauI GCGCGC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 2 cut(s) 468, 789
PctI GAATGC 2 cut(s) 356, 567
PfeI GAWTC 2 cut(s) 265, 329
PfoI TCCNGGA 1 cut(s) 101
PkrI GCNGC 4 cut(s) 202, 308, 466, 1052
PleI GAGTC 5 cut(s) 214, 403, 440, 940, 977
PpsI GAGTC 5 cut(s) 214, 403, 440, 940, 977
PpuMI RGGWCCY 2 cut(s) 508, 907
Psp124BI GAGCTC 1 cut(s) 658
Psp5II RGGWCCY 2 cut(s) 508, 907
Psp6I CCWGG 1 cut(s) 101
PspEI GGTNACC 2 cut(s) 109, 913
PspFI CCCAGC 2 cut(s) 121, 997
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 6 cut(s) 496, 510, 670, 732, 909, 1003
PspPI GGNCC 6 cut(s) 243, 255, 494, 508, 907, 1002
PspPPI RGGWCCY 2 cut(s) 508, 907
PstI CTGCAG 1 cut(s) 171
PsuI RGATCY 1 cut(s) 730
PteI GCGCGC 1 cut(s) 467
PvuII CAGCTG 2 cut(s) 203, 650
RsaI GTAC 3 cut(s) 639, 704, 938
RsaNI GTAC 3 cut(s) 638, 703, 937
RseI CAYNNNNRTG 1 cut(s) 285
Rsr2I CGGWCCG 1 cut(s) 255
RsrII CGGWCCG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 658
SacII CCGCGG 1 cut(s) 222
SatI GCNGC 4 cut(s) 201, 307, 465, 1051
Sau3AI GATC 6 cut(s) 147, 390, 547, 582, 730, 814
Sau96I GGNCC 6 cut(s) 243, 255, 494, 508, 907, 1002
SchI GAGTC 5 cut(s) 215, 404, 441, 941, 977
ScrFI CCNGG 4 cut(s) 103, 197, 911, 1021
SduI GDGCHC 3 cut(s) 594, 658, 1027
SfaNI GCATC 2 cut(s) 463, 880
SfcI CTRYAG 1 cut(s) 167
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 4 cut(s) 243, 255, 508, 907
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 2 cut(s) 159, 556
SmoI CTYRAG 2 cut(s) 159, 556
Sse9I AATT 5 cut(s) 87, 97, 115, 602, 712
SsiI CCGC 6 cut(s) 67, 219, 221, 258, 419, 779
SstI GAGCTC 1 cut(s) 658
StyD4I CCNGG 4 cut(s) 101, 195, 909, 1019
StyI CCWWGG 3 cut(s) 20, 427, 663
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 2 cut(s) 573, 795
TaqI TCGA 3 cut(s) 393, 573, 930
TasI AATT 5 cut(s) 87, 97, 115, 602, 712
TatI WGTACW 1 cut(s) 702
TfiI GAWTC 2 cut(s) 265, 329
TseFI GTSAC 2 cut(s) 27, 913
TseI GCWGC 4 cut(s) 200, 306, 464, 1050
Tsp45I GTSAC 2 cut(s) 27, 913
TspDTI ATGAA 4 cut(s) 33, 275, 471, 676
VpaK11BI GGWCC 4 cut(s) 243, 255, 508, 907
XapI RAATTY 2 cut(s) 97, 602
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 18
XmiI GTMKAC 1 cut(s) 981
ZraI GACGTC 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.