Rorug01G0183800

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002539
Physical Location & Seq
Forward (+)
25385353 .. 25504510
119158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0183800.1

Sequence Viewer

Length: 1029 bp
ATGTCTGATGATCAAAGATCCTCACTTTCCGGCAATGGCCAAACTGTGTGTGTTACTGGGGCCGGAGGCTACATCGCTTCTTGGATTGTCAAGCTCCTTCTGGAGAGAGGCTATCATGTGAGAGGAACTTCGAGAAACCCAGATGACCCAAAGAATGTTCATTTGAGAGAGCTGGAAGGAGCCAGAGAGAGGCTGATCAGCTTGAGAAAAGTCGATCTTCTCGATTTAGAGAGCCTTAAAGATGCCATTGCCGGCTGCGATGGCGTTTTCCACACAGCATCCCCTATAACTGATGATCCGGAAGAAATGGTGTCGCCGGCTGTGAATGGAACAAAGAATGTGATCGTTGCCGCTTCTGAAGCCAAGGTTAAACGCGTGGTCTTCACATCTTCAAACGGTGCCGTCTACATGAACCCCACCAGAGGTCCCGATGTGGTTGTCGATGAGTCCTGTTGGAGTGACCTGGAGTTTTGCAAGAACACCAAGAACTGGTACTGCTATGGGAAAACTGTGGCAGAGCTAGCAGCTTGGGATGAAGCCAAAGAGAGAGGGGTGGACTTGGTTGTGGTGAACCCAGTTTATGTGCTTGGACCACTGCTCCAACCCACCATCAACATTAGCACTGTCCACATCCTCAAGTACTTGACTGGCTCAGCTAAGACTTATGCCAATTCAATTCAGGCCTATGTGCATGTTAAGGATGTGGCATTAGCACACATACTAGTGTATGAAACTCCCTCTGCCTCCGGTCGTTATCTTTGCGGCGAGAGCGTCCTTCACCGCGGAGATGTGGTTGAAATCCTTGCCAAGTACTTCCCTGAATACCCAATACCCACCAAGTTGAAAGACGATGGGAAACCCAGAGCAAAACCATACAAGTTCTCAAACCAGAAGCTACAAGACTTGGGTTTGGAGTTCACCCCCGTGAAACAGAGCCTATATGACACTGTCAAGAGCTTGCAGGACAAGGGGCACCTTAAAGTTCCTACAAAACAAGAAGAAGACTCCATAATATTGGATCTTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

342

Amino Acids

37.71

Weight (kDa)

6.1

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 14 - 134 6.8e-13 NmrA-like family
Epimerase PF01370 16 - 249 1.3e-26 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 16 - 140 1.8e-07 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 18 - 242 4.5e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 18 - 266 1.3e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 20 - 136 3.8e-15 NAD(P)H-binding
NAD_binding_4 PF07993 77 - 201 8.8e-08 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 398, 972
AccB7I CCANNNNNTGG 1 cut(s) 489
AccI GTMKAC 1 cut(s) 405
AccII CGCG 2 cut(s) 375, 783
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 4 cut(s) 351, 762, 781, 783
AclWI GGATC 3 cut(s) 12, 290, 1026
AcoI YGGCCR 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 3 cut(s) 494, 641, 812
AfiI CCNNNNNNNGG 3 cut(s) 189, 422, 489
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 4 cut(s) 393, 675, 797, 844
AhlI ACTAGT 1 cut(s) 721
AjnI CCWGG 1 cut(s) 462
AleI CACNNNNGTG 1 cut(s) 923
AluBI AGCT 8 cut(s) 94, 172, 201, 520, 527, 656, 895, 957
AluI AGCT 8 cut(s) 94, 172, 201, 520, 527, 656, 895, 957
AlwI GGATC 3 cut(s) 12, 290, 1026
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 3 cut(s) 37, 60, 681
ApeKI GCWGC 2 cut(s) 255, 524
AspS9I GGNCC 3 cut(s) 60, 425, 590
AsuHPI GGTGA 3 cut(s) 580, 770, 910
AsuNHI GCTAGC 1 cut(s) 520
AvaII GGWCC 2 cut(s) 425, 590
BaeGI GKGCMC 1 cut(s) 975
BalI TGGCCA 1 cut(s) 39
BanI GGYRCC 2 cut(s) 398, 972
BbsI GAAGAC 2 cut(s) 373, 1008
BbvI GCAGC 2 cut(s) 242, 536
BccI CCATC 3 cut(s) 254, 617, 845
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 2 cut(s) 10, 195
BcuI ACTAGT 1 cut(s) 721
BfaI CTAG 2 cut(s) 521, 722
BisI GCNGC 4 cut(s) 256, 351, 525, 763
BlpI GCTNAGC 1 cut(s) 652
BlsI GCNGC 4 cut(s) 257, 352, 526, 764
BmcAI AGTACT 2 cut(s) 641, 812
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 2 cut(s) 425, 590
BmgT120I GGNCC 3 cut(s) 60, 425, 590
BmiI GGNNCC 5 cut(s) 61, 181, 400, 427, 974
BmrFI CCNGG 1 cut(s) 464
BmrI ACTGGG 2 cut(s) 66, 569
BmsI GCATC 2 cut(s) 232, 287
BmtI GCTAGC 1 cut(s) 524
BmuI ACTGGG 2 cut(s) 66, 569
BpiI GAAGAC 2 cut(s) 373, 1008
BpmI CTGGAG 2 cut(s) 122, 485
Bpu1102I GCTNAGC 1 cut(s) 652
BpuEI CTTGAG 2 cut(s) 223, 620
BsaJI CCNNGG 2 cut(s) 363, 781
BsaWI WCCGGW 2 cut(s) 298, 746
BsaXI ACNNNNNCTCC 4 cut(s) 582, 612, 777, 807
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 422, 489
Bse118I RCCGGY 2 cut(s) 251, 316
Bse1I ACTGG 4 cut(s) 61, 494, 575, 652
Bse3DI GCAATG 2 cut(s) 40, 246
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 2 cut(s) 363, 781
BseGI GGATG 4 cut(s) 278, 538, 630, 706
BseLI CCNNNNNNNGG 3 cut(s) 189, 422, 489
BseMI GCAATG 2 cut(s) 40, 246
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 4 cut(s) 61, 494, 575, 652
BseSI GKGCMC 1 cut(s) 975
BseXI GCAGC 2 cut(s) 242, 536
Bsh1236I CGCG 2 cut(s) 375, 783
Bsh1285I CGRYCG 1 cut(s) 751
BshFI GGCC 3 cut(s) 39, 62, 683
BshNI GGYRCC 2 cut(s) 398, 972
BsiEI CGRYCG 1 cut(s) 751
BsiSI CCGG 6 cut(s) 30, 63, 252, 299, 317, 747
BslFI GGGAC 1 cut(s) 411
BslI CCNNNNNNNGG 3 cut(s) 189, 422, 489
BsmFI GGGAC 1 cut(s) 411
BsnI GGCC 3 cut(s) 39, 62, 683
Bsp1286I GDGCHC 1 cut(s) 975
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
Bsp1720I GCTNAGC 1 cut(s) 652
BspACI CCGC 4 cut(s) 351, 762, 781, 783
BspANI GGCC 3 cut(s) 39, 62, 683
BspCNI CTCAG 1 cut(s) 665
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 2 cut(s) 375, 783
BspLI GGNNCC 5 cut(s) 61, 181, 400, 427, 974
BspOI GCTAGC 1 cut(s) 524
BspPI GGATC 3 cut(s) 12, 290, 1026
BspT107I GGYRCC 2 cut(s) 398, 972
BsrDI GCAATG 2 cut(s) 40, 246
BsrFI RCCGGY 2 cut(s) 251, 316
BsrI ACTGG 4 cut(s) 61, 494, 575, 652
BssAI RCCGGY 2 cut(s) 251, 316
BssECI CCNNGG 2 cut(s) 363, 781
BssMI GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 5 cut(s) 46, 398, 511, 625, 949
BstC8I GCNNGC 4 cut(s) 253, 318, 522, 959
BstDEI CTNAG 2 cut(s) 652, 657
BstDSI CCRYGG 1 cut(s) 781
BstF5I GGATG 4 cut(s) 278, 538, 630, 706
BstFNI CGCG 2 cut(s) 375, 783
BstKTI GATC 7 cut(s) 13, 20, 198, 217, 298, 345, 1021
BstMBI GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
BstMCI CGRYCG 1 cut(s) 751
BstMWI GCNNNNNNNGC 4 cut(s) 261, 359, 521, 768
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 1 cut(s) 462
BstSLI GKGCMC 1 cut(s) 975
BstUI CGCG 2 cut(s) 375, 783
BstV1I GCAGC 2 cut(s) 242, 536
BstV2I GAAGAC 2 cut(s) 373, 1008
BstX2I RGATCY 2 cut(s) 17, 1018
BstXI CCANNNNNNTGG 1 cut(s) 1015
BstYI RGATCY 2 cut(s) 17, 1018
BsuRI GGCC 3 cut(s) 39, 62, 683
BtgI CCRYGG 1 cut(s) 781
BtgZI GCGATG 2 cut(s) 58, 273
BtsCI GGATG 4 cut(s) 278, 538, 630, 706
BtsI GCAGTG 1 cut(s) 593
BtsIMutI CAGTG 3 cut(s) 593, 621, 945
Cac8I GCNNGC 4 cut(s) 253, 318, 522, 959
Cfr10I RCCGGY 2 cut(s) 251, 316
Cfr13I GGNCC 3 cut(s) 60, 425, 590
Cfr42I CCGCGG 1 cut(s) 784
CseI GACGC 1 cut(s) 760
Csp6I GTAC 3 cut(s) 493, 640, 811
CviAII CATG 3 cut(s) 116, 409, 692
CviQI GTAC 3 cut(s) 493, 640, 811
DdeI CTNAG 2 cut(s) 652, 657
DpnI GATC 7 cut(s) 12, 19, 197, 216, 297, 344, 1020
DpnII GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
EaeI YGGCCR 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 363
Eco147I AGGCCT 1 cut(s) 683
Eco47I GGWCC 2 cut(s) 425, 590
Eco57I CTGAAG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 3 cut(s) 119, 412, 695
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 1 cut(s) 411
FatI CATG 3 cut(s) 115, 408, 691
FbaI TGATCA 2 cut(s) 10, 195
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 4 cut(s) 256, 351, 525, 763
FokI GGATG 4 cut(s) 265, 545, 617, 713
Fsp4HI GCNGC 4 cut(s) 256, 351, 525, 763
FspBI CTAG 2 cut(s) 521, 722
GluI GCNGC 4 cut(s) 256, 351, 525, 763
GsuI CTGGAG 2 cut(s) 122, 485
HaeIII GGCC 3 cut(s) 39, 62, 683
HapII CCGG 6 cut(s) 30, 63, 252, 299, 317, 747
HgaI GACGC 1 cut(s) 760
Hin1II CATG 3 cut(s) 119, 412, 695
HinfI GANTC 2 cut(s) 446, 1004
HpaII CCGG 6 cut(s) 30, 63, 252, 299, 317, 747
HphI GGTGA 3 cut(s) 580, 770, 910
Hpy166II GTNNAC 5 cut(s) 406, 556, 571, 628, 918
Hpy188I TCNGA 2 cut(s) 7, 358
Hpy188III TCNNGA 6 cut(s) 101, 132, 221, 299, 428, 952
Hpy8I GTNNAC 5 cut(s) 406, 556, 571, 628, 918
HpyAV CCTTC 3 cut(s) 107, 170, 785
HpyCH4III ACNGT 5 cut(s) 46, 398, 511, 625, 949
HpyCH4V TGCA 3 cut(s) 474, 691, 961
HpyF10VI GCNNNNNNNGC 4 cut(s) 261, 359, 521, 768
HpyF3I CTNAG 2 cut(s) 652, 657
Hsp92II CATG 3 cut(s) 119, 412, 695
Kpn2I TCCGGA 1 cut(s) 298
KroI GCCGGC 2 cut(s) 251, 316
KroNI GCCGGC 2 cut(s) 253, 318
Ksp22I TGATCA 2 cut(s) 10, 195
KspI CCGCGG 1 cut(s) 784
Kzo9I GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
LmnI GCTCC 3 cut(s) 99, 179, 603
Lsp1109I GCAGC 2 cut(s) 242, 536
LweI GCATC 2 cut(s) 232, 287
MaeI CTAG 2 cut(s) 521, 722
MaeIII GTNAC 2 cut(s) 52, 458
MalI GATC 7 cut(s) 12, 19, 197, 216, 297, 344, 1020
MboI GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
MboII GAAGA 6 cut(s) 209, 314, 373, 381, 1010, 1013
MflI RGATCY 2 cut(s) 17, 1018
MhlI GDGCHC 1 cut(s) 975
MlsI TGGCCA 1 cut(s) 39
MluCI AATT 2 cut(s) 670, 675
MluI ACGCGT 1 cut(s) 373
MluNI TGGCCA 1 cut(s) 39
MlyI GAGTC 2 cut(s) 455, 998
MmeI TCCRAC 2 cut(s) 434, 625
Mox20I TGGCCA 1 cut(s) 39
MroI TCCGGA 1 cut(s) 298
MroNI GCCGGC 2 cut(s) 251, 316
MscI TGGCCA 1 cut(s) 39
MseI TTAA 5 cut(s) 237, 369, 696, 978, 1027
MslI CAYNNNNRTG 2 cut(s) 722, 923
Msp20I TGGCCA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 783
MspI CCGG 6 cut(s) 30, 63, 252, 299, 317, 747
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
MvnI CGCG 2 cut(s) 375, 783
MwoI GCNNNNNNNGC 4 cut(s) 261, 359, 521, 768
NaeI GCCGGC 2 cut(s) 253, 318
NdeII GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
NgoMIV GCCGGC 2 cut(s) 251, 316
NheI GCTAGC 1 cut(s) 520
NlaIII CATG 3 cut(s) 119, 412, 695
NlaIV GGNNCC 5 cut(s) 61, 181, 400, 427, 974
NmuCI GTSAC 1 cut(s) 458
NspI RCATGY 1 cut(s) 695
OliI CACNNNNGTG 1 cut(s) 923
PceI AGGCCT 1 cut(s) 683
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PdiI GCCGGC 2 cut(s) 253, 318
PflFI GACNNNGTC 1 cut(s) 947
PflMI CCANNNNNTGG 1 cut(s) 489
PkrI GCNGC 4 cut(s) 257, 352, 526, 764
PleI GAGTC 2 cut(s) 454, 998
PpsI GAGTC 2 cut(s) 454, 998
PpuMI RGGWCCY 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 5 cut(s) 61, 181, 400, 427, 974
PspPI GGNCC 3 cut(s) 60, 425, 590
PspPPI RGGWCCY 1 cut(s) 425
PsuI RGATCY 2 cut(s) 17, 1018
PsyI GACNNNGTC 1 cut(s) 947
RsaI GTAC 3 cut(s) 494, 641, 812
RsaNI GTAC 3 cut(s) 493, 640, 811
RseI CAYNNNNRTG 2 cut(s) 722, 923
SacII CCGCGG 1 cut(s) 784
SaqAI TTAA 5 cut(s) 237, 369, 696, 978, 1027
SatI GCNGC 4 cut(s) 256, 351, 525, 763
Sau3AI GATC 7 cut(s) 10, 17, 195, 214, 295, 342, 1018
Sau96I GGNCC 3 cut(s) 60, 425, 590
ScaI AGTACT 2 cut(s) 641, 812
SchI GAGTC 2 cut(s) 455, 998
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 1 cut(s) 975
SfaNI GCATC 2 cut(s) 232, 287
Sfr303I CCGCGG 1 cut(s) 784
SgrBI CCGCGG 1 cut(s) 784
SinI GGWCC 2 cut(s) 425, 590
SmiMI CAYNNNNRTG 2 cut(s) 722, 923
SmlI CTYRAG 2 cut(s) 202, 635
SmoI CTYRAG 2 cut(s) 202, 635
SpeI ACTAGT 1 cut(s) 721
Sse9I AATT 2 cut(s) 670, 675
SseBI AGGCCT 1 cut(s) 683
SsiI CCGC 4 cut(s) 351, 762, 781, 783
SspI AATATT 1 cut(s) 1014
SspMI CTAG 2 cut(s) 521, 722
StuI AGGCCT 1 cut(s) 683
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 5 cut(s) 46, 398, 511, 625, 949
TaqI TCGA 4 cut(s) 131, 213, 222, 441
TasI AATT 2 cut(s) 670, 675
TatI WGTACW 2 cut(s) 639, 810
TauI GCSGC 2 cut(s) 353, 765
Tru1I TTAA 5 cut(s) 237, 369, 696, 978, 1027
Tru9I TTAA 5 cut(s) 237, 369, 696, 978, 1027
TscAI CASTG 3 cut(s) 600, 628, 952
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 2 cut(s) 255, 524
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 4 cut(s) 149, 425, 549, 744
TspRI CASTG 3 cut(s) 600, 628, 952
Tth111I GACNNNGTC 1 cut(s) 947
Van91I CCANNNNNTGG 1 cut(s) 489
VpaK11BI GGWCC 2 cut(s) 425, 590
XceI RCATGY 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 405
XspI CTAG 2 cut(s) 521, 722
ZrmI AGTACT 2 cut(s) 641, 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.