Rroxscaffold_5G00369410

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003456
Physical Location & Seq
Forward (+)
51241172 .. 51242857
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369410.1

Sequence Viewer

Length: 1311 bp
ATGGCGTCGTTCCAGTCTGAGTTACTAAATGAGTTGAGGCAATTTCACTTGGAACTTATAGCTCAGGTGATGGAGCAGTCGGGGCCGACTTCGTCCCCGAACAGTTCCCCTGCATCATTTTGCTCGGTAAGTGGCGCTAACACAAGTGCTTCTAATCCGTTCAATTGTCACAAGGATGATTGTGGTGGTAATGGTATTGATGAGAAATTAGAGAATGGGAAGAGTGTCGAGAAGAGGGCTGAGGTGACTAGTGGAGATGGTAGAGAGTTCAGTGAGCTTGAGTGTATCATTGCTAAGCAAGACGAGGGTGGTCTAGTTGAAGATAAGGAGCAGCTTAGCAATGGAATGTCAGAATTTCATGCCCTCCAACTGTTTGGTAAAATGCCAAAGTTAGATTGGTTGTGTAATGTAAGAAGGAACATTGCTGCTGTTATGAGCATTCATGACAAAGATGATAGAGTTGATAAATCAAATGTGCTTCTTGGTGATGTTGAATTAAATTTCACTACCCACTTACTTGGTCCAGATGATAATGAAAAAGATGGAAACTGTGAGGGGAGTGAAGAGTTTGGAAACCACAGCAGAAAGGGACTCGTAGAAGAAAATCTTGTGGCAAATCCTTTTGAGCAGATTTCTGCTTGTGTGCCTGAAGTTAGTAACCAGGGACTCTCAATTAATAATTTCGACCTGAATGGAAAGTCGTATCCCCTAATATGGGGAGGAGATGTTGCTAATTTCTCTGCTGGAGCTAACTCGGAGATTGTAAGCTTTTGTCCAAATGGAGCCCTGAATTCACACAAAGTAAAGGGAAAGATTGTTTTCTGTGAAACCATAGGCTATGGTTCGGGTGTTTTACTAGCCAATGGTGTTGGTACAATTATGGCTTATTCATCTTCCTCTGATTTGGCACTTAATTACCCGTTAGCAGCAACTGTGGTTAAAATAGGAGATGGACAGAGAATTATGAACTATATCAGATCAACAGCGAATCCAAATGCAACAATTCTTGTTGGCGAGACGGACACGGATATCATGGCACGTGTTGTTGTGTCATTTTCTTCTAGAGGACCCAACCCCATAACCCCAGATATCCTCAAGCTTGATTTGACAGCCCCTGGTTTTGACATACTTGCTGCTTGGTGTCTAGTGGCACCGCCTTCCATTTACGAAGATGACACCAGGAGTGTCGAATTTAATGTTATCTTCGGGATATCCATGTCCTGCCCTCATGCTAGTGGTGCTGCAGCTTATGTCAAGGCTGCACATCCAAATTGGTCTCCTGCTGCAAAACAGTGCATTGGGAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

436

Amino Acids

46.72

Weight (kDa)

4.82

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 325 - 429 9.6e-11 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1150
AciI CCGC 1 cut(s) 1154
AcsI RAATTY 4 cut(s) 353, 499, 790, 1190
AcuI CTGAAG 1 cut(s) 669
AcvI CACGTG 1 cut(s) 1040
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 874
AfiI CCNNNNNNNGG 2 cut(s) 714, 715
AflIII ACRYGT 1 cut(s) 1039
AgsI TTSAA 3 cut(s) 163, 320, 494
AhlI ACTAGT 1 cut(s) 248
AjnI CCWGG 3 cut(s) 660, 1114, 1178
AluBI AGCT 7 cut(s) 62, 277, 334, 749, 768, 1099, 1247
AluI AGCT 7 cut(s) 62, 277, 334, 749, 768, 1099, 1247
Alw26I GTCTC 2 cut(s) 1010, 1281
AlwNI CAGNNNCTG 2 cut(s) 932, 1115
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 8 cut(s) 331, 425, 926, 1133, 1241, 1244, 1259, 1283
ApoI RAATTY 4 cut(s) 353, 499, 790, 1190
AseI ATTAAT 1 cut(s) 675
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 83, 521, 1067
AsuHPI GGTGA 3 cut(s) 79, 256, 497
AvaII GGWCC 2 cut(s) 521, 1067
BanI GGYRCC 1 cut(s) 1150
BanII GRGCYC 1 cut(s) 787
BbrPI CACGTG 1 cut(s) 1040
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 8 cut(s) 343, 412, 938, 1120, 1228, 1246, 1256, 1270
BccI CCATC 4 cut(s) 64, 251, 536, 944
BciT130I CCWGG 3 cut(s) 662, 1116, 1180
BciVI GTATCC 1 cut(s) 714
BcoDI GTCTC 2 cut(s) 1010, 1281
BcuI ACTAGT 1 cut(s) 248
BfaI CTAG 6 cut(s) 249, 314, 857, 1062, 1145, 1233
BfmI CTRYAG 1 cut(s) 1242
BfoI RGCGCY 1 cut(s) 138
BfuI GTATCC 1 cut(s) 714
BisI GCNGC 8 cut(s) 332, 426, 927, 1134, 1242, 1245, 1260, 1284
BlpI GCTNAGC 2 cut(s) 294, 335
BlsI GCNGC 8 cut(s) 333, 427, 928, 1135, 1243, 1246, 1261, 1285
Bme1390I CCNGG 3 cut(s) 662, 1116, 1180
Bme18I GGWCC 2 cut(s) 521, 1067
BmgT120I GGNCC 3 cut(s) 83, 521, 1067
BmiI GGNNCC 4 cut(s) 84, 784, 1069, 1152
BmrFI CCNGG 3 cut(s) 662, 1116, 1180
BmsI GCATC 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 765
Bpu10I CCTNAGC 2 cut(s) 63, 240
Bpu1102I GCTNAGC 2 cut(s) 294, 335
BpuEI CTTGAG 2 cut(s) 299, 1079
BsaAI YACGTR 1 cut(s) 1040
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1281
BsaJI CCNNGG 2 cut(s) 661, 1114
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 2 cut(s) 714, 715
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 3 cut(s) 288, 346, 420
BseBI CCWGG 3 cut(s) 662, 1116, 1180
BseDI CCNNGG 2 cut(s) 661, 1114
BseGI GGATG 2 cut(s) 181, 1264
BseLI CCNNNNNNNGG 2 cut(s) 714, 715
BseMI GCAATG 3 cut(s) 288, 346, 420
BseMII CTCAG 3 cut(s) 9, 77, 231
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 735
BseXI GCAGC 8 cut(s) 343, 412, 938, 1120, 1228, 1246, 1256, 1270
BsgI GTGCAG 1 cut(s) 1245
BshFI GGCC 1 cut(s) 85
BshNI GGYRCC 1 cut(s) 1150
BslFI GGGAC 3 cut(s) 79, 603, 678
BslI CCNNNNNNNGG 2 cut(s) 714, 715
BsmAI GTCTC 2 cut(s) 1010, 1281
BsmBI CGTCTC 1 cut(s) 1010
BsmFI GGGAC 3 cut(s) 79, 603, 678
BsmI GAATGC 1 cut(s) 438
BsnI GGCC 1 cut(s) 85
Bso31I GGTCTC 1 cut(s) 1281
Bsp1286I GDGCHC 1 cut(s) 787
Bsp143I GATC 1 cut(s) 977
Bsp1720I GCTNAGC 2 cut(s) 294, 335
BspACI CCGC 1 cut(s) 1154
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 3 cut(s) 10, 76, 232
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 4 cut(s) 84, 784, 1069, 1152
BspMAI CTGCAG 1 cut(s) 1246
BspT107I GGYRCC 1 cut(s) 1150
BspTNI GGTCTC 1 cut(s) 1281
BsrDI GCAATG 3 cut(s) 288, 346, 420
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 661, 1114
BssMI GATC 1 cut(s) 977
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 3 cut(s) 662, 1116, 1180
Bst4CI ACNGT 5 cut(s) 104, 372, 551, 934, 1293
Bst6I CTCTTC 3 cut(s) 215, 227, 558
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 1 cut(s) 1040
BstDEI CTNAG 5 cut(s) 18, 63, 240, 294, 335
BstF5I GGATG 2 cut(s) 181, 1264
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 1 cut(s) 980
BstMAI GTCTC 2 cut(s) 1010, 1281
BstMBI GATC 1 cut(s) 977
BstMWI GCNNNNNNNGC 2 cut(s) 82, 1238
BstNI CCWGG 3 cut(s) 662, 1116, 1180
BstSCI CCNGG 3 cut(s) 660, 1114, 1178
BstSFI CTRYAG 1 cut(s) 1242
BstV1I GCAGC 8 cut(s) 343, 412, 938, 1120, 1228, 1246, 1256, 1270
BstXI CCANNNNNNTGG 2 cut(s) 374, 518
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 1 cut(s) 85
BtsCI GGATG 2 cut(s) 181, 1264
BtsIMutI CAGTG 2 cut(s) 277, 1298
CaiI CAGNNNCTG 2 cut(s) 932, 1115
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 137
Cfr13I GGNCC 3 cut(s) 83, 521, 1067
Csp6I GTAC 1 cut(s) 873
CviAII CATG 5 cut(s) 359, 443, 1033, 1216, 1229
CviQI GTAC 1 cut(s) 873
DdeI CTNAG 5 cut(s) 18, 63, 240, 294, 335
DpnI GATC 1 cut(s) 979
DpnII GATC 1 cut(s) 977
Eam1104I CTCTTC 3 cut(s) 215, 227, 558
EarI CTCTTC 3 cut(s) 215, 227, 558
Eco24I GRGCYC 1 cut(s) 787
Eco31I GGTCTC 1 cut(s) 1281
Eco32I GATATC 3 cut(s) 1030, 1090, 1212
Eco47I GGWCC 2 cut(s) 521, 1067
Eco57I CTGAAG 1 cut(s) 669
Eco72I CACGTG 1 cut(s) 1040
EcoO109I RGGNCCY 1 cut(s) 1067
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 3 cut(s) 660, 1114, 1178
EcoRV GATATC 3 cut(s) 1030, 1090, 1212
EcoT38I GRGCYC 1 cut(s) 787
Esp3I CGTCTC 1 cut(s) 1010
FaeI CATG 5 cut(s) 362, 446, 1036, 1219, 1232
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 3 cut(s) 79, 603, 678
FatI CATG 5 cut(s) 358, 442, 1032, 1215, 1228
Fnu4HI GCNGC 8 cut(s) 332, 426, 927, 1134, 1242, 1245, 1260, 1284
FokI GGATG 2 cut(s) 188, 1251
FriOI GRGCYC 1 cut(s) 787
Fsp4HI GCNGC 8 cut(s) 332, 426, 927, 1134, 1242, 1245, 1260, 1284
FspBI CTAG 6 cut(s) 249, 314, 857, 1062, 1145, 1233
GlaI GCGC 1 cut(s) 136
GluI GCNGC 8 cut(s) 332, 426, 927, 1134, 1242, 1245, 1260, 1284
GsuI CTGGAG 1 cut(s) 765
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 1 cut(s) 85
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 362, 446, 1036, 1219, 1232
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HindIII AAGCTT 2 cut(s) 766, 1097
HinfI GANTC 3 cut(s) 591, 666, 988
HphI GGTGA 3 cut(s) 79, 256, 497
Hpy188I TCNGA 5 cut(s) 19, 352, 757, 901, 977
Hpy188III TCNNGA 5 cut(s) 229, 443, 524, 1062, 1207
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 408, 1167
HpyCH4III ACNGT 5 cut(s) 104, 372, 551, 934, 1293
HpyCH4IV ACGT 1 cut(s) 1039
HpyCH4V TGCA 6 cut(s) 113, 998, 1244, 1262, 1286, 1296
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 1238
HpyF3I CTNAG 5 cut(s) 18, 63, 240, 294, 335
HpySE526I ACGT 1 cut(s) 1039
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 362, 446, 1036, 1219, 1232
HspAI GCGC 1 cut(s) 135
Kzo9I GATC 1 cut(s) 977
LmnI GCTCC 4 cut(s) 73, 328, 746, 782
Lsp1109I GCAGC 8 cut(s) 343, 412, 938, 1120, 1228, 1246, 1256, 1270
LweI GCATC 1 cut(s) 122
MaeI CTAG 6 cut(s) 249, 314, 857, 1062, 1145, 1233
MaeII ACGT 1 cut(s) 1039
MaeIII GTNAC 4 cut(s) 21, 167, 244, 656
MalI GATC 1 cut(s) 979
MboI GATC 1 cut(s) 977
MboII GAAGA 9 cut(s) 232, 244, 332, 575, 611, 885, 1050, 1181, 1195
MfeI CAATTG 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 787
MlyI GAGTC 2 cut(s) 585, 660
MmeI TCCRAC 1 cut(s) 391
MseI TTAA 5 cut(s) 497, 675, 912, 939, 1194
MslI CAYNNNNRTG 2 cut(s) 174, 1233
MspR9I CCNGG 3 cut(s) 662, 1116, 1180
MunI CAATTG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 438
MvaI CCWGG 3 cut(s) 662, 1116, 1180
MwoI GCNNNNNNNGC 2 cut(s) 82, 1238
NdeII GATC 1 cut(s) 977
NlaIII CATG 5 cut(s) 362, 446, 1036, 1219, 1232
NlaIV GGNNCC 4 cut(s) 84, 784, 1069, 1152
NmuCI GTSAC 2 cut(s) 167, 244
PagI TCATGA 1 cut(s) 442
PctI GAATGC 1 cut(s) 438
PfeI GAWTC 1 cut(s) 988
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 8 cut(s) 333, 427, 928, 1135, 1243, 1246, 1261, 1285
PleI GAGTC 2 cut(s) 585, 660
PmaCI CACGTG 1 cut(s) 1040
PmlI CACGTG 1 cut(s) 1040
PpsI GAGTC 2 cut(s) 585, 660
Ppu21I YACGTR 1 cut(s) 1040
PpuMI RGGWCCY 1 cut(s) 1067
PshBI ATTAAT 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 1067
Psp6I CCWGG 3 cut(s) 660, 1114, 1178
PspCI CACGTG 1 cut(s) 1040
PspGI CCWGG 3 cut(s) 660, 1114, 1178
PspN4I GGNNCC 4 cut(s) 84, 784, 1069, 1152
PspPI GGNCC 3 cut(s) 83, 521, 1067
PspPPI RGGWCCY 1 cut(s) 1067
PstI CTGCAG 1 cut(s) 1246
PstNI CAGNNNCTG 2 cut(s) 932, 1115
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 1 cut(s) 874
RsaNI GTAC 1 cut(s) 873
RseI CAYNNNNRTG 2 cut(s) 174, 1233
SaqAI TTAA 5 cut(s) 497, 675, 912, 939, 1194
SatI GCNGC 8 cut(s) 332, 426, 927, 1134, 1242, 1245, 1260, 1284
Sau3AI GATC 1 cut(s) 977
Sau96I GGNCC 3 cut(s) 83, 521, 1067
SchI GAGTC 2 cut(s) 585, 660
ScrFI CCNGG 3 cut(s) 662, 1116, 1180
SduI GDGCHC 1 cut(s) 787
SfaNI GCATC 1 cut(s) 122
SfcI CTRYAG 1 cut(s) 1242
SinI GGWCC 2 cut(s) 521, 1067
SmiMI CAYNNNNRTG 2 cut(s) 174, 1233
SmlI CTYRAG 2 cut(s) 278, 1094
SmoI CTYRAG 2 cut(s) 278, 1094
SpeI ACTAGT 1 cut(s) 248
SsiI CCGC 1 cut(s) 1154
SspMI CTAG 6 cut(s) 249, 314, 857, 1062, 1145, 1233
StyD4I CCNGG 3 cut(s) 660, 1114, 1178
TaaI ACNGT 5 cut(s) 104, 372, 551, 934, 1293
TaiI ACGT 1 cut(s) 1042
TaqI TCGA 3 cut(s) 228, 684, 1188
TfiI GAWTC 1 cut(s) 988
Tru1I TTAA 5 cut(s) 497, 675, 912, 939, 1194
Tru9I TTAA 5 cut(s) 497, 675, 912, 939, 1194
TscAI CASTG 2 cut(s) 277, 1298
TseFI GTSAC 2 cut(s) 167, 244
TseI GCWGC 8 cut(s) 331, 425, 926, 1133, 1241, 1244, 1259, 1283
Tsp45I GTSAC 2 cut(s) 167, 244
TspDTI ATGAA 5 cut(s) 347, 431, 549, 879, 980
TspGWI ACGGA 3 cut(s) 147, 1034, 1040
TspRI CASTG 2 cut(s) 277, 1298
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 521, 1067
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 4 cut(s) 353, 499, 790, 1190
XbaI TCTAGA 1 cut(s) 1061
XcmI CCANNNNNNNNNTGG 1 cut(s) 393
XspI CTAG 6 cut(s) 249, 314, 857, 1062, 1145, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.