Rroxscaffold_5G00373360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001795
Physical Location & Seq
Reverse (-)
54613876 .. 54616969
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373360.1

Sequence Viewer

Length: 393 bp
ATGTCTCTCTTAGATGATGCTGGAGGTCCGTTAGTGTTTGCCCTCCTCGGGGTCGGCGCCAAGATCCGCCACCTCACCCGCCACGTCGGCGCCAAGGTCCGCAAGCTCGACCTCGCCCGGCCGCTCATCAACTCCACGCTCCTCCTTTTGGATGCGATTGTGGAGCTCGACCTCGCCCGCTCTGTTGATCCTCTTGATCTTGGCAATCTCGGCCACCGTGAGGCCTTGAAAGACCCTCTTCATCTCCTACTACTCCGTCTCCCTCAAGCGCCTCCCCTCCTCTTCCTCCTCCTCGGGATTCGTCACCGAGATCGGAGACCTCAAGCCCATCTGTTTCGACACGGCGTTTTGGTCCGGCCGGACGCCGCGGCCTCTGCCGATGAGGCCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.26

Weight (kDa)

10.32

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 56, 89
AccBSI CCGCTC 2 cut(s) 124, 180
AccII CGCG 1 cut(s) 368
AciI CCGC 8 cut(s) 67, 79, 100, 122, 178, 366, 368, 388
AclWI GGATC 2 cut(s) 58, 182
AcoI YGGCCR 3 cut(s) 119, 211, 356
AcyI GRCGYC 3 cut(s) 57, 90, 363
AfiI CCNNNNNNNGG 4 cut(s) 48, 49, 148, 220
AgsI TTSAA 1 cut(s) 229
AjiI CACGTC 1 cut(s) 85
AluBI AGCT 2 cut(s) 106, 166
AluI AGCT 2 cut(s) 106, 166
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 3 cut(s) 9, 263, 310
AlwI GGATC 2 cut(s) 58, 182
Ama87I CYCGRG 2 cut(s) 47, 293
AoxI GGCC 6 cut(s) 119, 211, 222, 356, 369, 384
AspLEI GCGC 3 cut(s) 59, 92, 271
AspS9I GGNCC 4 cut(s) 26, 97, 352, 385
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 67, 296
AvaI CYCGRG 2 cut(s) 47, 293
AvaII GGWCC 3 cut(s) 26, 97, 352
BanI GGYRCC 2 cut(s) 56, 89
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 168
BccI CCATC 1 cut(s) 336
BceAI ACGGC 1 cut(s) 358
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 3 cut(s) 9, 263, 310
BfaI CTAG 1 cut(s) 391
BfoI RGCGCY 3 cut(s) 60, 93, 272
BglI GCCNNNNNGGC 2 cut(s) 87, 383
BisI GCNGC 3 cut(s) 122, 366, 369
BlsI GCNGC 3 cut(s) 123, 367, 370
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 3 cut(s) 26, 97, 352
BmeT110I CYCGRG 2 cut(s) 47, 293
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 4 cut(s) 26, 97, 352, 385
BmiI GGNNCC 2 cut(s) 58, 91
BmrFI CCNGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 7, 142
BpmI CTGGAG 1 cut(s) 42
BpuEI CTTGAG 2 cut(s) 249, 306
BpuMI CCSGG 1 cut(s) 118
BsaHI GRCGYC 3 cut(s) 57, 90, 363
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 4 cut(s) 46, 93, 292, 366
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 49, 148, 220
BseDI CCNNGG 4 cut(s) 46, 93, 292, 366
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 4 cut(s) 48, 49, 148, 220
BseRI GAGGAG 5 cut(s) 35, 131, 269, 278, 281
BseX3I CGGCCG 2 cut(s) 119, 356
Bsh1236I CGCG 1 cut(s) 368
Bsh1285I CGRYCG 2 cut(s) 122, 359
BshFI GGCC 6 cut(s) 121, 213, 224, 358, 371, 386
BshNI GGYRCC 2 cut(s) 56, 89
BsiEI CGRYCG 2 cut(s) 122, 359
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 2 cut(s) 47, 293
BsiSI CCGG 3 cut(s) 118, 355, 359
BslI CCNNNNNNNGG 4 cut(s) 48, 49, 148, 220
BsmAI GTCTC 3 cut(s) 9, 263, 310
BsmBI CGTCTC 1 cut(s) 263
BsnI GGCC 6 cut(s) 121, 213, 224, 358, 371, 386
Bso31I GGTCTC 1 cut(s) 310
BsoBI CYCGRG 2 cut(s) 47, 293
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 4 cut(s) 63, 187, 196, 310
BspACI CCGC 8 cut(s) 67, 79, 100, 122, 178, 366, 368, 388
BspANI GGCC 6 cut(s) 121, 213, 224, 358, 371, 386
BspFNI CGCG 1 cut(s) 368
BspLI GGNNCC 2 cut(s) 58, 91
BspPI GGATC 2 cut(s) 58, 182
BspT107I GGYRCC 2 cut(s) 56, 89
BspTNI GGTCTC 1 cut(s) 310
BsrBI CCGCTC 2 cut(s) 124, 180
BssECI CCNNGG 4 cut(s) 46, 93, 292, 366
BssMI GATC 4 cut(s) 63, 187, 196, 310
BssNI GRCGYC 3 cut(s) 57, 90, 363
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 1 cut(s) 218
Bst6I CTCTTC 2 cut(s) 243, 287
BstACI GRCGYC 3 cut(s) 57, 90, 363
BstC8I GCNNGC 3 cut(s) 104, 178, 388
BstDEI CTNAG 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 157
BstFNI CGCG 1 cut(s) 368
BstH2I RGCGCY 3 cut(s) 60, 93, 272
BstHHI GCGC 3 cut(s) 59, 92, 271
BstKTI GATC 4 cut(s) 66, 190, 199, 313
BstMAI GTCTC 3 cut(s) 9, 263, 310
BstMBI GATC 4 cut(s) 63, 187, 196, 310
BstMCI CGRYCG 2 cut(s) 122, 359
BstMWI GCNNNNNNNGC 4 cut(s) 87, 210, 374, 383
BstSCI CCNGG 1 cut(s) 116
BstUI CGCG 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
BstZI CGGCCG 2 cut(s) 119, 356
BsuRI GGCC 6 cut(s) 121, 213, 224, 358, 371, 386
BtgI CCRYGG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 157
Cac8I GCNNGC 3 cut(s) 104, 178, 388
CfoI GCGC 3 cut(s) 59, 92, 271
Cfr13I GGNCC 4 cut(s) 26, 97, 352, 385
Cfr42I CCGCGG 1 cut(s) 369
CseI GACGC 1 cut(s) 371
CviJI RGCY 9 cut(s) 106, 121, 166, 213, 224, 326, 358, 371, 386
CviKI_1 RGCY 9 cut(s) 106, 121, 166, 213, 224, 326, 358, 371, 386
DdeI CTNAG 1 cut(s) 10
DinI GGCGCC 2 cut(s) 58, 91
DpnI GATC 4 cut(s) 65, 189, 198, 312
DpnII GATC 4 cut(s) 63, 187, 196, 310
EaeI YGGCCR 3 cut(s) 119, 211, 356
EagI CGGCCG 2 cut(s) 119, 356
Eam1104I CTCTTC 2 cut(s) 243, 287
EarI CTCTTC 2 cut(s) 243, 287
EciI GGCGGA 1 cut(s) 56
Ecl136II GAGCTC 1 cut(s) 166
EclXI CGGCCG 2 cut(s) 119, 356
Eco130I CCWWGG 1 cut(s) 93
Eco147I AGGCCT 1 cut(s) 224
Eco24I GRGCYC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 310
Eco47I GGWCC 3 cut(s) 26, 97, 352
Eco52I CGGCCG 2 cut(s) 119, 356
Eco53kI GAGCTC 1 cut(s) 166
Eco88I CYCGRG 2 cut(s) 47, 293
EcoICRI GAGCTC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 1 cut(s) 168
EgeI GGCGCC 2 cut(s) 58, 91
EheI GGCGCC 2 cut(s) 58, 91
ErhI CCWWGG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 263
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FauI CCCGC 2 cut(s) 86, 185
Fnu4HI GCNGC 3 cut(s) 122, 366, 369
FokI GGATG 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 122, 366, 369
FspBI CTAG 1 cut(s) 391
GlaI GCGC 3 cut(s) 58, 91, 270
GluI GCNGC 3 cut(s) 122, 366, 369
GsuI CTGGAG 1 cut(s) 42
HaeII RGCGCY 3 cut(s) 60, 93, 272
HaeIII GGCC 6 cut(s) 121, 213, 224, 358, 371, 386
HapII CCGG 3 cut(s) 118, 355, 359
HgaI GACGC 1 cut(s) 371
HhaI GCGC 3 cut(s) 59, 92, 271
Hin1I GRCGYC 3 cut(s) 57, 90, 363
Hin6I GCGC 3 cut(s) 57, 90, 269
HinP1I GCGC 3 cut(s) 57, 90, 269
HinfI GANTC 1 cut(s) 298
HpaII CCGG 3 cut(s) 118, 355, 359
HphI GGTGA 2 cut(s) 67, 296
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 2 cut(s) 194, 295
Hpy99I CGWCG 1 cut(s) 89
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 210, 374, 383
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 3 cut(s) 57, 90, 363
HspAI GCGC 3 cut(s) 57, 90, 269
KasI GGCGCC 2 cut(s) 56, 89
KspI CCGCGG 1 cut(s) 369
Kzo9I GATC 4 cut(s) 63, 187, 196, 310
LmnI GCTCC 2 cut(s) 144, 163
LpnPI CCDG 4 cut(s) 6, 131, 368, 372
LweI GCATC 2 cut(s) 7, 142
MaeI CTAG 1 cut(s) 391
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 302
MalI GATC 4 cut(s) 65, 189, 198, 312
MbiI CCGCTC 2 cut(s) 124, 180
MboI GATC 4 cut(s) 63, 187, 196, 310
MboII GAAGA 2 cut(s) 230, 274
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 168
Mly113I GGCGCC 2 cut(s) 57, 90
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 3 cut(s) 118, 355, 359
MspR9I CCNGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 4 cut(s) 87, 210, 374, 383
NarI GGCGCC 2 cut(s) 57, 90
NciI CCSGG 1 cut(s) 118
NdeII GATC 4 cut(s) 63, 187, 196, 310
NlaIV GGNNCC 2 cut(s) 58, 91
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 302
PceI AGGCCT 1 cut(s) 224
PfeI GAWTC 1 cut(s) 298
PkrI GCNGC 3 cut(s) 123, 367, 370
PluTI GGCGCC 2 cut(s) 60, 93
Psp124BI GAGCTC 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 58, 91
PspPI GGNCC 4 cut(s) 26, 97, 352, 385
PsuI RGATCY 1 cut(s) 63
SacI GAGCTC 1 cut(s) 168
SacII CCGCGG 1 cut(s) 369
SatI GCNGC 3 cut(s) 122, 366, 369
Sau3AI GATC 4 cut(s) 63, 187, 196, 310
Sau96I GGNCC 4 cut(s) 26, 97, 352, 385
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 1 cut(s) 168
SetI ASST 9 cut(s) 28, 75, 87, 99, 108, 114, 168, 174, 322
SfaNI GCATC 2 cut(s) 7, 142
SfoI GGCGCC 2 cut(s) 58, 91
Sfr303I CCGCGG 1 cut(s) 369
SgrBI CCGCGG 1 cut(s) 369
SinI GGWCC 3 cut(s) 26, 97, 352
SmlI CTYRAG 2 cut(s) 264, 321
SmoI CTYRAG 2 cut(s) 264, 321
SseBI AGGCCT 1 cut(s) 224
SsiI CCGC 8 cut(s) 67, 79, 100, 122, 178, 366, 368, 388
SspDI GGCGCC 2 cut(s) 56, 89
SspMI CTAG 1 cut(s) 391
SstI GAGCTC 1 cut(s) 168
StuI AGGCCT 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 218
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 108, 168, 337
TauI GCSGC 3 cut(s) 124, 368, 371
TfiI GAWTC 1 cut(s) 298
TseFI GTSAC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 230
TspGWI ACGGA 2 cut(s) 18, 245
VpaK11BI GGWCC 3 cut(s) 26, 97, 352
XspI CTAG 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.